Protein Overview: DAS1

General Information:
Name(s) found:	DAS1 / YJL149W [SGD]
Description(s) found: Found 26 descriptions. SHOW ALL	Putative SCF ubiquitin ligase F-box protein; interacts physically with both Cdc53p and Skp1 and genetically with CDC34; similar to putative F-box protein YDR131C [SGD]
Organism:	Saccharomyces cerevisiae
Length:	663 amino acids
Gene Ontology:
Cellular Component:	SCF ubiquitin ligase complex [IPI]
Biological Process:	biological_process [ND]
Molecular Function:	molecular_function [ND]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MPFQDYFQKK KAAFINRNNK SNADASALRD INDININFAA KSKNYVFPLT KLPDELMQEV  60
   61 FSHLPQPDRL QLCLVNKRLN KIATKLLYRR IYLNDSNVVK SDFMHLAINW TLLNLPSSLK 120
  121 EEESRDIANC KLKKLIETLQ NNIHITEVIQ WIRINWDLDS TLQRSILSIL CNQGKSLQRL 180
  181 ENVTDPACND IISNGHFSRS NVSSFDMAPP NSLPEMVVPE NYIPNLTKYL SQRISSRLSH 240
  241 MTLFIDPLKL FNYLYPLDIK LQIIDLKLHW RREFYNNDYF VKKIRPGNPL TKLSEVFDKR 300
  301 TLKILTIISW NDTLLKRETE MLKDFKEFEN LEDLSLISIK QDVHILVDLF SSLTNLKRLK 360
  361 MDFLEEYVPE PTNPHIFLSI LLACSKLQFI DLRYDGLIPQ IINIQENKFQ LNQQCNCTNC 420
  421 QIVFSDILKG KIFMFPEDYY IHDLQDIAAK DIFKMMKYLS LLPYSKACDA YPSVRTQPMN 480
  481 LTNFVTKMNR NLLEYRNSKS QLVPKIVNNP HQHSTVTSTS TAHMSEPEMI IIDDDDDDDE 540
  541 INAAIPPSSD DTAATISTDL ELPHESLTKR DIIMCYHALI HHFKSIYVTF LKSFPHLRFL 600
  601 MLNDIPTIVM EENNERIFEP VFYHYDYKSN LYGWSKESNK NLENDSNNNN NNSDTIARIA 660
  661 TVM

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Ho Y, et al. (2002)		CDC53 DAS1 SKP1

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	MLP2	#29 Asynchronous Prep5-TiO2 Flowthrough	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	DAS1	AST2	1	Unpublished Fields Lab Data
View Screen	DAS1	IMD2	1	Unpublished Fields Lab Data
View Screen	DAS1	MPS3	1	Unpublished Fields Lab Data
View Screen	DAS1	GEF1	1	Unpublished Fields Lab Data
View Screen	DAS1	DID4	1	Unpublished Fields Lab Data
View Screen	DAS1	MBR1	1	Unpublished Fields Lab Data
View Screen	DAS1	YLR152C	1	Unpublished Fields Lab Data
View Screen	DAS1	LCB1	1	Unpublished Fields Lab Data
View Screen	DAS1	YNL208W	1	Unpublished Fields Lab Data
View Screen	DAS1	ZIM17	1	Unpublished Fields Lab Data
View Screen	DAS1	MED7	1	Unpublished Fields Lab Data
View Screen	GIR2	DAS1	1	Unpublished Fields Lab Data
View Screen	DAS1	MLC2	1	Unpublished Fields Lab Data
View Screen	DAS1	LUC7	1	Unpublished Fields Lab Data
View Screen	DAS1	NRG1	1	Unpublished Fields Lab Data
View Screen	DAS1	YDR357C	1	Unpublished Fields Lab Data
View Screen	DAS1	UTR4	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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No microscopy data found in the PDR for this protein.

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..103]	Pfam	6.387216	F-box domain No confident structure predictions are available.
2	View Details	[104..663]	deduced	N/A	No confident structure predictions are available.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2