Protein Overview: ECM14

General Information:
Name(s) found:	ECM14 / YHR132C [SGD]
Description(s) found: Found 25 descriptions. SHOW ALL	Putative metalloprotease with similarity to the zinc carboxypeptidase family, required for normal cell wall assembly [SGD]
Organism:	Saccharomyces cerevisiae
Length:	430 amino acids
Gene Ontology:
Cellular Component:	fungal-type vacuole [IDA]
Biological Process:	proteolysis [ISS] cellular cell wall organization [IMP]
Molecular Function:	metalloendopeptidase activity [ISS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MLHMNSLWGC FLFVLLAVTG AVQGLQEDYS EYAVYRFTSD NYSTLVRDVI APLTDDYDVW  60
   61 TRSNNFIDIK LPKEIGEQIN DGQVIIDNMN ELIQNTLPTS QMMAREQAVF ENDYDFFFNE 120
  121 YRDLDTIYMW LDLLERSFPS LVAVEHLGRT FEGRELKALH ISGNKPESNP EKKTIVITGG 180
  181 IHAREWISVS TVCWALYQLL NRYGSSKKET KYLDDLDFLV IPVFNPDGYA YTWSHDRLWR 240
  241 KNRQRTHVPQ CLGIDIDHSF GFQWEKAHTH ACSEEYSGET PFEAWEASAW YKYINETKGD 300
  301 YKIYGYIDMH SYSQEILYPY AYSCDALPRD LENLLELSYG LSKAIRSKSG RNYDVISACK 360
  361 DRGSDIFPGL GAGSALDFMY HHRAHWAFQL KLRDTGNHGF LLPPENIKPV GKETYAALKY 420
  421 FCDFLLDPEI

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Qiu et al. (2008)		ECM14 PRC1 VMA9

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	VAM3	Sample: HX12 - trans-SNARE complex formation and fusion are inhibited (control).	Hao Xu, et al. (2010)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

No yeast two-hybrid interactions found for this protein.

	PROTEIN(S)	PUBLICATION
View Data	ECM14	Huh WK, et al. (2003)

Searching the YRC Public Image Repository, found results:

Currently showing to of results.

[View Search in the YRC Public Image Repository]

Searching the YRC Public Image Repository, found no results.

Ginzu Version Information

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

Gene Ontology predictions

Term	Confidence	Notes
peptidase activity	4.786910294594	bayes_pls_golite062009
peptidase activity, acting on L-amino acid peptides	4.40766568731738	bayes_pls_golite062009
hydrolase activity	4.23257784904124	bayes_pls_golite062009
exopeptidase activity	3.28577934113028	bayes_pls_golite062009
metallopeptidase activity	2.76103081380105	bayes_pls_golite062009
carboxypeptidase activity	2.63658263814587	bayes_pls_golite062009
metalloexopeptidase activity	1.76058821499096	bayes_pls_golite062009
metallocarboxypeptidase activity	1.56827526553175	bayes_pls_golite062009
catalytic activity	1.3989281287879	bayes_pls_golite062009
binding	1.18091528890016	bayes_pls_golite062009
endopeptidase activity	0.910234727191613	bayes_pls_golite062009
serine hydrolase activity	0.596149133958797	bayes_pls_golite062009
serine-type peptidase activity	0.578572340123331	bayes_pls_golite062009
nucleic acid binding	0.328807457631995	bayes_pls_golite062009
protein binding	0.215930537243465	bayes_pls_golite062009
aminopeptidase activity	0.0966237570696103	bayes_pls_golite062009

No functions predicted.

Source: Reynolds et al. 2008. Manuscript submitted

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..89]	PSI-BLAST	583.0103	Carboxypeptidase A; Procarboxypeptidase A
2	View Details	[90..430]	PSI-BLAST	583.0103	Carboxypeptidase A; Procarboxypeptidase A

Protein Overview: ECM14

Protein Complex Data

Mass Spectrometry Data

Yeast Two-Hybrid Data

Microscopy / Localization Data

Protein Structure Data

Philius Transmembrane Prediction: