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Protein Overview: CBF2

General Information:
Name(s) found: CBF2 / YGR140W [SGD]
Description(s) found:
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  • gi|6321579|ref|NP_011656.1| Essential kinetochore protein, component of the CBF3 multisubunit complex that binds to the CDEIII region of the centromere; Cbf2p also binds to the CDEII region possibly forming a different multimeric complex, ubiquitinated in [NCBI NR]
  • gi|416757|sp|P32504.1|CBF3A_YEAST RecName: Full=Centromere DNA-binding protein complex CBF3 subunit A; AltName: Full=Chromosome transmission fidelity protein 14; AltName: Full=Kinetochore protein CTF14; AltName: Full=Centromere-binding factor 2 [NCBI NR]
  • gi|297174|emb|CAA49158.1| NDC10 [Saccharomyces cerevisiae] [NCBI NR]
  • pir||S64449 centromere-binding protein complex 110K chain - yeast (Saccharomyces cerevisiae) [NCBI NR]
  • gi|1323234|emb|CAA97153.1| CBF2 [Saccharomyces cerevisiae] [NCBI NR]
  • Centromere DNA-binding protein complex CBF3 subunit A OS=Saccharomyces cerevisiae GN=CBF2 PE=1 SV=1 [Swiss-Prot]
  • (P32504) Centromere DNA-binding protein complex CBF3 subunit A (Kinetochore protein CTF14) (Centromere-binding factor 2) [Swiss-Prot]
  • CBF2 Kinetochore protein complex CBF3, 110 KD subunit [mips]
  • Essential kinetochore protein, component of the CBF3 multisubunit complex that binds to the CDEIII region of the centromere; Cbf2p also binds to the CDEII region possibly forming a different multimeric complex, ubiquitinated in vivo [SGD]
  • CBF2, Chr VII from 767424-770294 [yeast_orfs2]
  • Chr VII from 767424-770294 [yeastorf3.fasta]
  • Chr VII from 767431-770301 [yeastorf4.fasta]
  • CBF2 SGDID:S0003372, Chr VII from 767432-770302, Verified ORF [yeastflysequest.fasta]
  • CBF2 SGDID:S0003372, Chr VII from 767434-770304, Verified ORF [10112005cervSGD.fasta]
  • Cbf2p [Saccharomyces cerevisiae]gi|1323234|emb|CAA97153.1| CBF2 [Saccharomyces cerevisiae]gi|297174|emb|CAA49158.1| NDC10 [Saccharomyces cerevisiae]gi|2131209|pir||S64449 centromere-binding protein complex 110K chain - yeast (Saccharomyces cerevisiae) [nrdb_11152004_Chlamydomonas]
  • CBF2 SGDID:S000003372, Chr VII from 767434-770304, Verified ORF, "Essential kinetochore protein, component of the CBF3 multisubunit complex that binds to the CDEIII region of the centromere; Cbf2p also binds to the CDEII region possibly forming a differen [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta]
  • Essential kinetochore protein, component of the CBF3 multisubunit complex that binds to the CDEIII region of the centromere; Cbf2p also binds to the CDEII region possibly forming a different multimeric complex, ubiquitinated in vivo; Cbf2p [Saccharomyces [nr-271106-contam.fasta]
  • NDC10 [Saccharomyces cerevisiae] [nr-271106-contam.fasta]
  • CBF2 [Saccharomyces cerevisiae] [nr-271106-contam.fasta]
  • Centromere DNA-binding protein complex CBF3 subunit A (Kinetochore protein CTF14) (Centromere-binding factor 2) [nr-271106-contam.fasta]
  • CBF2 SGDID:S000003372, Chr VII from 767434-770304, Verified ORF, "Essential kinetochore protein, component of the CBF3 multisubunit complex that binds to the CDEIII region of the centromere; Cbf2p also binds to the CDEII region possibly forming a different multimeric complex, ubiquitinated in vivo" [yeast-bovrabprots.fasta]
  • [chemostatdb.fasta]
  • Centromere DNA-binding protein complex CBF3 subunit A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=CBF2 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta]
  • CBF2 SGDID:S000003372, Chr VII from 767429-770299, Genome Release 64-1-1, Verified ORF, "Essential kinetochore protein, component of the CBF3 multisubunit complex that binds to the CDEIII region of the centromere; Cbf2p also binds to the CDEII region possibly forming a different multimeric complex, ubiquitinated in vivo" [yeast-030211-contam.fasta]
  • CBF2 SGDID:S000003372, Chr VII from 767429-770299, Genome Release 64-2-1, Verified ORF, "Essential kinetochore protein; component of the CBF3 multisubunit complex that binds to the CDEIII region of the centromere; Cbf2p also binds to the CDEII region possibly forming a different multimeric complex, ubiquitinated in vivo; sumoylated in an Mms21p-dependent manner; relative distribution to the spindle pole body decreases upon DNA replication stress" [20141208_orf_trans.fasta]
  • YGR140W SGDID:S000003372, Chr VII from 767429-770299, Genome Release 64-1-1, Verified ORF, "Essential kinetochore protein, component of the CBF3 multisubunit complex that binds to the CDEIII region of the centromere; Cbf2p also binds to the CDEII region possibly forming a different multimeric complex, ubiquitinated in vivo" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
    • Organism: Saccharomyces cerevisiae
    Length: 956 amino acids

    Gene Ontology:
    Cellular Component: spindle midzone [IDALink to PubMed article]
    CBF3 complex [IDALink to PubMed articleLink to PubMed articleLink to PubMed article]
    spindle [IDALink to PubMed article]
    condensed nuclear chromosome kinetochore [IDALink to PubMed article][TASLink to PubMed article]
    Biological Process: mitotic spindle elongation [IMPLink to PubMed article]
    chromosome segregation [IMPLink to PubMed article]
    Molecular Function: DNA bending activity [IDALink to PubMed article]
    centromeric DNA binding [IDALink to PubMed article]


    Sequence:
    Sequence:
    [PDR BLAST]
    [ProtParam] 
          1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MRSSILFLLK LMKIMDVQQQ QEAMSSEDRF QELVDSLKPR TAHQYKTYYT KYIQWCQLNQ  60
   61 IIPTPEDNSV NSVPYKDLPI SAELIHWFLL DTLITDDKPG EKREETEDLD EEEENSFKIA 120
  121 TLKKIIGSLN FLSKLCKVHE NPNANIDTKY LESVTKLHTH WIDSQKAITT NETNNTNTQV 180
  181 LCPPLLKVSL NLWNPETNHL SEKFFKTCSE KLRFLVDFQL RSYLNLSFEE RSKIRFGSLK 240
  241 LGKRDRDAII YHKVTHSAEK KDTPGHHQLL ALLPQDCPFI CPQTTLAAYL YLRFYGIPSV 300
  301 SKGDGFPNLN ADENGSLLQD IPILRGKSLT TYPREETFSN YYTTVFRYCH LPYKRREYFN 360
  361 KCNLVYPTWD EDTFRTFFNE ENHGNWLEQP EAFAFPDKIP FDFKKIMNFK SPYTSYSTNA 420
  421 KKDPFPPPKD LLVQIFPEID EYKRHDYEGL SQNSRDFLDL MEVLRERFLS NLPWIYKFFP 480
  481 NHDIFQDPIF GNSDFQSYFN DKTIHSKGSP ILSFDILPGF NKIYKNKTNF YSLLIERPSQ 540
  541 LTFASSHNPD THPTQKQESE GPLQMSQLDT TQLNELLKQQ SFEYVQFQTL SNFQILLSVF 600
  601 NKIFEKLEMK KSSRGYILHQ LNLFKITLDE RIKKSKIDDA DKFIRDNQPI KKEENIVNED 660
  661 GPNTSRRTKR PKQIRLLSIA DSSDESSTED SNVFKKDGES IEDGAYGENE DENDSEMQEQ 720
  721 LKSMINELIN SKISTFLRDQ MDQFELKINA LLDKILEEKV TRIIEQKLGS HTGKFSTLKR 780
  781 PQLYMTEEHN VGFDMEVPKK LRTSGKYAET VKDNDDHQAM STTASPSPEQ DQEAKSYTDE 840
  841 QEFMLDKSID SIEGIILEWF TPNAKYANQC VHSMNKSGNK SWRANCEALY KERKSIVEFY 900
  901 IYLVNHESLD RYKAVDICEK LRDQNEGSFS RLAKFLRKWR HDHQNSFDGL LVYLSN

    Protein Complex Data

      PUBLICATION TOPOLOGY COCOMPLEXED PROTEINS
    View Details (MIPS) Mewes HW, et al. (2004) CBF2 CEP3 CTF13 SKP1
    View Details Krogan NJ, et al. (2006) ALG3 BUD27 CBF2 DST1 ESS1 IWR1 RPB10 RPB11 RPB2 RPB3 RPB4 RPB5 RPB7 RPB8 RPB9 RPO21 RPO26 SPT4 TAF14 TFG1 TFG2 YBL059W YBR259W YPL225W
    View Details Qiu et al. (2008) CBF2 CEP3 SKP1

    Mass Spectrometry Data

    The following runs contain data for this protein:

      BAIT COMMENTS PUBLICATION
    View Run NSL1 No Comments Hazbun TR, et al. (2003)
    View Run BIR1 No Comments Widlund PO, et al. (2005)
    View Run CEP3 sample cbf3 from feb 2005 Sandall S, et all (2006)

    Yeast Two-Hybrid Data

    The following interactions contain this protein:

    NOT SHOWING SINGLE HITS. [ Show Single Hits ]

    [View our yeast two-hybrid interpretation guidelines.]

    No yeast two-hybrid interactions found for this protein.

    Microscopy / Localization Data

    New Feature: Upload Your Own Microscopy Data

      PROTEIN(S) PUBLICATION
    View Data CBF2 and SPC42 Huh WK, et al. (2003)
    View Data CBF2 Huh WK, et al. (2003)

    Protein Structure Data


    [What does the above image mean?]
    [View Top Sequence Alignments] [Show Ginzu Version Information]

    Domains predicted:

    #   Region(s) Method Confidence Match Description
    1 View Details [1..956] deduced N/A No confident structure predictions are available.
    2 View Details [488..670] deduced N/A No confident structure predictions are available.
    3 View Details [671..825] deduced N/A No confident structure predictions are available.
    4 View Details [826..956] deduced N/A No confident structure predictions are available.

    Functions predicted (by domain):

    # Gene Ontology predictions
    1
    Term Confidence Notes
  • binding
    		•  1.73696253244735 bayes_pls_golite062009
  • catalytic activity
    		•  1.16839016519994 bayes_pls_golite062009
  • double-stranded DNA binding
    		•  0.897470387532799 bayes_pls_golite062009
  • structure-specific DNA binding
    		•  0.893619260648623 bayes_pls_golite062009
  • protein binding
    		•  0.593345606025678 bayes_pls_golite062009
  • nucleic acid binding
    		•  0.446493119279535 bayes_pls_golite062009
  • DNA binding
    		•  0.398540590517261 bayes_pls_golite062009
    2 No functions predicted.
    3 No functions predicted.
    4 No functions predicted.


    Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076


    Philius Transmembrane Prediction:

    Protein predicted to be: GLOBULAR (No transmembrane regions or signal peptide)
    Confidence of classification: 0.92

    Source: Reynolds et al. (2008)

    YRC Informatics Platform - Version 3.0
    Created and Maintained by: Michael Riffle