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Protein Overview: ASN2

General Information:
Name(s) found: ASN2 / YGR124W [SGD]
Description(s) found:
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  • gi|642347|emb|CAA58159.1| glutamic-dependent asparagine synthase [Saccharomyces cerevisiae] [NCBI NR]
  • gi|6321563|ref|NP_011640.1| Asparagine synthetase, isozyme of Asn1p; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway; Asn2p [Saccharomyces cerevisiae] [NCBI NR]
  • gi|1351986|sp|P49090.2|ASNS2_YEAST RecName: Full=Asparagine synthetase [glutamine-hydrolyzing] 2; AltName: Full=Glutamine-dependent asparagine synthetase 2 [NCBI NR]
  • gi|1323203|emb|CAA97135.1| ASN2 [Saccharomyces cerevisiae] [NCBI NR]
  • pir||S55982 asparagine synthase (glutamine-hydrolysing) (EC 6.3.5.4) - yeast (Saccharomyces cerevisiae) [NCBI NR]
  • Asparagine synthetase [glutamine-hydrolyzing] 2 OS=Saccharomyces cerevisiae GN=ASN2 PE=1 SV=2 [Swiss-Prot]
  • ASN2 Asparagine synthetase [mips]
  • Asparagine synthetase, isozyme of Asn1p; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway [SGD]
  • ASN2, Chr VII from 739939-741657 [yeast_orfs2]
  • Chr VII from 739939-741657 [yeastorf3.fasta]
  • Chr VII from 739946-741664 [yeastorf4.fasta]
  • ASN2 SGDID:S0003356, Chr VII from 739947-741665, Verified ORF [yeastflysequest.fasta]
  • ASN2 SGDID:S0003356, Chr VII from 739949-741667, Verified ORF [10112005cervSGD.fasta]
  • Asn2p [Saccharomyces cerevisiae]gi|1323203|emb|CAA97135.1| ASN2 [Saccharomyces cerevisiae]gi|642347|emb|CAA58159.1| glutamic-dependent asparagine synthase [Saccharomyces cerevisiae]gi|1351986|sp|P49090|ASN2_YEAST Asparagine synthetase [glutamine-hydrol [nrdb_11152004_Chlamydomonas]
  • ASN2 SGDID:S000003356, Chr VII from 739949-741667, Verified ORF, "Asparagine synthetase, isozyme of Asn1p; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta]
  • Asparagine synthetase [glutamine-hydrolyzing] 2 (Glutamine-dependent asparagine synthetase 2) [nr-271106-contam.fasta]
  • ASN2 [Saccharomyces cerevisiae] [nr-271106-contam.fasta]
  • Asparagine synthetase, isozyme of Asn1p; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway; Asn2p [Saccharomyces cerevisiae] [nr-271106-contam.fasta]
  • glutamic-dependent asparagine synthase [Saccharomyces cerevisiae] [nr-271106-contam.fasta]
  • [chemostatdb.fasta]
  • Asparagine synthetase [glutamine-hydrolyzing] 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=ASN2 PE=1 SV=2 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta]
  • ASN2 SGDID:S000003356, Chr VII from 739944-741662, Genome Release 64-1-1, Verified ORF, "Asparagine synthetase, isozyme of Asn1p; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway" [yeast-030211-contam.fasta]
  • ASN2 SGDID:S000003356, Chr VII from 739944-741662, Genome Release 64-2-1, Verified ORF, "Asparagine synthetase; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway; ASN2 has a paralog, ASN1, that arose from the whole genome duplication" [20141208_orf_trans.fasta]
  • YGR124W SGDID:S000003356, Chr VII from 739944-741662, Genome Release 64-1-1, Verified ORF, "Asparagine synthetase, isozyme of Asn1p; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
    • Organism: Saccharomyces cerevisiae
    Length: 572 amino acids

    Gene Ontology:
    Cellular Component: cytoplasm [IDALink to PubMed article]
    Biological Process: asparagine biosynthetic process [TASLink to PubMed article]
    Molecular Function: asparagine synthase (glutamine-hydrolyzing) activity [IMPLink to PubMed article][ISSLink to PubMed article]


    Sequence:
    Sequence:
    [PDR BLAST]
    [ProtParam] 
          1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MCGIFAAFKH EDIHNFKPKA LQLSKKIRHR GPDWSGNAVM NSTIFVHERL AIVGLDSGAQ  60
   61 PITSADGEYM LGVNGEIYNH IQLREMCSDY KFQTFSDCEP IIPLYLEHDI DAPKYLDGMF 120
  121 AFCLYDSKKD RIVAARDPIG VVTLYMGRSS QSPETVYFAS ELKCLTDVCD SIISFPPGHV 180
  181 YDSETDKITR YFTPDWLDEK RIPSTPVDYH AIRHSLEKAV RKRLMAEVPY GVLLSGGLDS 240
  241 SLIAAIAARE TEKANADANE DNNVDEKQLA GIDDQGHLHT SGWSRLHSFA IGLPNAPDLQ 300
  301 AARKVAKFIG SIHHEHTFTL QEGLDALDDV IYHLETYDVT TIRASTPMFL LSRKIKAQGV 360
  361 KMVLSGEGSD EIFGGYLYFA QAPSAAEFHT ESVQRVKNLH LADCLRANKS TMAWGLEARV 420
  421 PFLDKDFLQL CMNIDPNEKM IKPKEGRIEK YILRKAFDTT DEPDVKPYLP EEILWRQKEQ 480
  481 FSDGVGYSWI DGLRDTAERA ISDAMFANPK ADWGDDIPTT KEAYWYRLKF DAWFPQKTAA 540
  541 DTVMRWIPKA DWGCAEDPSG RYAKIHEKHV SA

    Protein Complex Data

      PUBLICATION TOPOLOGY COCOMPLEXED PROTEINS
    View Details Krogan NJ, et al. (2006) ASN2 STT3 URA7
    View Details Qiu et al. (2008) ASN1 ASN2

    Mass Spectrometry Data

    The following runs contain data for this protein:

      BAIT COMMENTS PUBLICATION
    View Run RPA135 No Comments Schneider, DA, et al. (2006)
    View Run MLP2 #25 Asynchronous Prep3-TiO2 Flowthrough Keck JM, et al. (2011)
    View Run MLP2 #24 Asynchronous Prep3-TiO2 Phosphopeptide enrichment Keck JM, et al. (2011)
    View Run MLP2 #32 Asynchronous Prep-No Phosphopeptide enrichment Keck JM, et al. (2011)
    View Run MLP2 #04 Alpha Factor Prep2 Keck JM, et al. (2011)

    Yeast Two-Hybrid Data

    The following interactions contain this protein:

    SHOWING SINGLE HITS. [ Hide Single Hits ]

    [View our yeast two-hybrid interpretation guidelines.]

      BAIT PREY HITS PUBLICATION
    View Screen HBT1 ASN2 1 Unpublished Fields Lab Data
    View Screen RAD9 ASN2 1 Unpublished Fields Lab Data
    View Screen RGD2 ASN2 1 Unpublished Fields Lab Data

    Microscopy / Localization Data

    New Feature: Upload Your Own Microscopy Data

      PROTEIN(S) PUBLICATION
    View Data ASN2 Huh WK, et al. (2003)

    Protein Structure Data


    [What does the above image mean?]
    [View Top Sequence Alignments] [Show Ginzu Version Information]

    Domains predicted:

    #   Region(s) Method Confidence Match Description
    1 View Details [1..192] PSI-BLAST 1646.0 Asparagine synthetase B, C-terminal domain; Asparagine synthetase B, N-terminal domain
    2 View Details [193..572] PSI-BLAST 1646.0 Asparagine synthetase B, C-terminal domain; Asparagine synthetase B, N-terminal domain

    Functions predicted (by domain):

    # Gene Ontology predictions
    1
    Term Confidence Notes
  • asparagine synthase (glutamine-hydrolyzing) activity
    		•  3.86770527955716 bayes_pls_golite062009
  • asparaginase activity
    		•  2.94567124791739 bayes_pls_golite062009
  • peptidase activity
    		•  1.76298553502805 bayes_pls_golite062009
  • DNA binding
    		•  1.66643360311025 bayes_pls_golite062009
  • nucleic acid binding
    		•  1.65897778831304 bayes_pls_golite062009
  • endopeptidase activity
    		•  1.58381740825719 bayes_pls_golite062009
  • peptidase activity, acting on L-amino acid peptides
    		•  1.56290563467621 bayes_pls_golite062009
  • acid-ammonia (or amide) ligase activity
    		•  1.41109692769794 bayes_pls_golite062009
  • N4-(beta-N-acetylglucosaminyl)-L-asparaginase activity
    		•  1.2528157874993 bayes_pls_golite062009
  • catalytic activity
    		•  1.19663085909195 bayes_pls_golite062009
  • binding
    		•  1.18139971903068 bayes_pls_golite062009
  • carbon-nitrogen ligase activity, with glutamine as amido-N-donor
    		•  1.17174228126736 bayes_pls_golite062009
  • hydrolase activity
    		•  1.05106912722792 bayes_pls_golite062009
  • protein binding
    		•  1.03373728327879 bayes_pls_golite062009
    2 No functions predicted.


    Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076


    Philius Transmembrane Prediction:

    Protein predicted to be: GLOBULAR (No transmembrane regions or signal peptide)
    Confidence of classification: 0.97

    Source: Reynolds et al. (2008)

    YRC Informatics Platform - Version 3.0
    Created and Maintained by: Michael Riffle