Protein Overview: CAK1

General Information:
Name(s) found:	CAK1 / YFL029C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|836725\|dbj\|BAA09209.1\| unnamed protein product [Saccharomyces cerevisiae] [NCBI NR] gi\|1480664\|gb\|AAB17052.1\| Cdk-activating kinase [NCBI NR] gi\|14318490\|ref\|NP_116624.1\| Cyclin-dependent kinase-activating kinase required for passage through the cell cycle, phosphorylates and activates Cdc28p; nucleotide-binding pocket differs significantly from those of most other protein kinases; Cak1p [Sacch [NCBI NR] gi\|1170645\|sp\|P43568.1\|CAK1_YEAST RecName: Full=Serine/threonine-protein kinase CAK1; AltName: Full=CDK-activating kinase [NCBI NR] pir\|\|S56225 hypothetical protein YFL029c - yeast (Saccharomyces cerevisiae) [NCBI NR] Serine/threonine-protein kinase CAK1 OS=Saccharomyces cerevisiae GN=CAK1 PE=1 SV=1 [Swiss-Prot] CAK1 Cdk-activating protein kinase [mips] Cyclin-dependent kinase-activating kinase required for passage through the cell cycle, phosphorylates and activates Cdc28p; nucleotide-binding pocket differs significantly from those of most other protein kinases [SGD] CAK1, Chr VI from 78053-79159, reverse complement [yeast_orfs2] Chr VI from 78053-79159, reverse complement [yeastorf3.fasta] Chr VI from 79159-78053, reverse complement [yeastorf5.fasta] (P43568) Serine/threonine-protein kinase CAK1 (EC 2.7.1.-) (CDK-activating kinase) [4932.FASTACCERV.txt] CAK1 SGDID:S0001865, Chr VI from 79159-78053, reverse complement, Verified ORF [yeastflysequest.fasta] Cak1p [Saccharomyces cerevisiae]gi\|1170645\|sp\|P43568\|CAK1_YEAST Serine/threonine-protein kinase CAK1 (CDK-activating kinase)gi\|1084693\|pir\|\|S56225 hypothetical protein YFL029c - yeast (Saccharomyces cerevisiae)gi\|1480664\|gb\|AAB17052.1\| Cdk-activating k [nrdb_11152004_Chlamydomonas] (P43568) Serine/threonine-protein kinase CAK1 (EC 2.7.1.37) (CDK-activating kinase) [cerv4932.FASTAC] CAK1 SGDID:S000001865, Chr VI from 79159-78053, reverse complement, Verified ORF, "Cyclin-dependent kinase-activating kinase required for passage through the cell cycle, phosphorylates and activates Cdc28p; nucleotide-binding pocket differs significantly [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] Cdk-activating kinase [nr-271106-contam.fasta] Cyclin-dependent kinase-activating kinase required for passage through the cell cycle, phosphorylates and activates Cdc28p; nucleotide-binding pocket differs significantly from those of most other protein kinases; Cak1p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] unnamed protein product [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Serine/threonine-protein kinase CAK1 (CDK-activating kinase) [nr-271106-contam.fasta] CAK1 SGDID:S000001865, Chr VI from 79159-78053, reverse complement, Verified ORF, "Cyclin-dependent kinase-activating kinase required for passage through the cell cycle, phosphorylates and activates Cdc28p; nucleotide-binding pocket differs significantly from those of most other protein kinases" [yeast-bovrabprots.fasta] [chemostatdb.fasta] Serine/threonine-protein kinase CAK1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=CAK1 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] CAK1 SGDID:S000001865, Chr VI from 79161-78055, Genome Release 64-1-1, reverse complement, Verified ORF, "Cyclin-dependent kinase-activating kinase required for passage through the cell cycle, phosphorylates and activates Cdc28p; nucleotide-binding pocket differs significantly from those of most other protein kinases" [yeast-030211-contam.fasta] CAK1 SGDID:S000001865, Chr VI from 79161-78055, Genome Release 64-2-1, reverse complement, Verified ORF, "Cyclin-dependent kinase-activating kinase; required for passage through the cell cycle; phosphorylates and activates Cdc28p; nucleotide-binding pocket differs significantly from those of most other protein kinases" [20141208_orf_trans.fasta] YFL029C SGDID:S000001865, Chr VI from 79161-78055, Genome Release 64-1-1, reverse complement, Verified ORF, "Cyclin-dependent kinase-activating kinase required for passage through the cell cycle, phosphorylates and activates Cdc28p; nucleotide-binding pocket differs significantly from those of most other protein kinases" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	368 amino acids
Gene Ontology:
Cellular Component:	cytoplasm [IDA]
Biological Process:	G2/M transition of mitotic cell cycle [IMP ] G1 phase of mitotic cell cycle [IMP ] protein amino acid phosphorylation [IDA ][IMP ] meiosis [IGI][IMP]
Molecular Function:	cyclin-dependent protein kinase activating kinase activity [IDA ][IMP ][IPI]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MKLDSIDITH CQLVKSTRTA RIYRSDTYAI KCLALDFDIP PHNAKFEVSI LNKLGNKCKH  60
   61 ILPLLESKAT DNNDLLLLFP FEEMNLYEFM QMHYKRDRRK KNPYYDLLNP SIPIVADPPV 120
  121 QKYTNQLDVN RYSLSFFRQM VEGIAFLHEN KIIHRDIKPQ NIMLTNNTST VSPKLYIIDF 180
  181 GISYDMANNS QTSAEPMDSK VTDISTGIYK APEVLFGVKC YDGGVDVWSL LIIISQWFQR 240
  241 ETSRMGHVPA MIDDGSDDMN SDGSDFRLIC SIFEKLGIPS IQKWEEVAQH GSVDAFVGMF 300
  301 GADGDGKYVL DQEKDVQISI VERNMPRLDE IADVKVKQKF INCILGMVSF SPNERWSCQR 360
  361 ILQELEKP

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Krogan NJ, et al. (2006)		CAK1 CDC34 CDC53 CDC9 CRT10 GRR1 MEP3 MET30 MMS1 POR1 RTT101 SAF1

Mass Spectrometry Data

No mass spectrometry results found for this protein.

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	CDC28	CAK1	2	Unpublished Fields Lab Data
View Screen	CDC28	CAK1	2	Unpublished Fields Lab Data
View Screen	VPS5	CAK1	2	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	CAK1	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..81]	PSI-BLAST	243.9794	Cyclin-dependent PK, CDK2
2	View Details	[82..204]	PSI-BLAST	243.9794	Cyclin-dependent PK, CDK2
3	View Details	[205..271]	PSI-BLAST	243.9794	Cyclin-dependent PK, CDK2
4	View Details	[272..368]	PSI-BLAST	243.9794	Cyclin-dependent PK, CDK2

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2