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Protein Overview: SAP1

General Information:
Name(s) found: SAP1 / YER047C [SGD]
Description(s) found:
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  • gi|731461|sp|P39955.1|SAP1_YEAST RecName: Full=Protein SAP1; AltName: Full=SIN1-associated protein [NCBI NR]
  • gi|6320887|ref|NP_010966.1| Putative ATPase of the AAA family, interacts with the Sin1p transcriptional repressor in the two-hybrid system; Sap1p [Saccharomyces cerevisiae] [NCBI NR]
  • gi|603280|gb|AAB64582.1| Yer047cp [Saccharomyces cerevisiae] [NCBI NR]
  • pir||S50550 SIN1-associated protein SAP1 - yeast (Saccharomyces cerevisiae) [NCBI NR]
  • Protein SAP1 OS=Saccharomyces cerevisiae GN=SAP1 PE=1 SV=1 [Swiss-Prot]
  • SAP1 Member of the AAA-protein family [mips]
  • Putative ATPase of the AAA family, interacts with the Sin1p transcriptional repressor in the two-hybrid system [SGD]
  • SAP1, Chr V from 243809-246502, reverse complement [yeast_orfs2]
  • Chr V from 243809-246502, reverse complement [yeastorf3.fasta]
  • Chr V from 246502-243809, reverse complement [yeastorf5.fasta]
  • (P39955) SAP1 protein [4932.FASTACCERV.txt]
  • SAP1 SGDID:S0000849, Chr V from 246502-243809, reverse complement, Verified ORF [yeastflysequest.fasta]
  • Putative ATPase of the AAA family, interacts with the Sin1p transcriptional repressor in the two-hybrid system [Saccharomyces cerevisiae]gi|731461|sp|P39955|SAP1_YEAST SAP1 proteingi|1077655|pir||S50550 SIN1-associated protein SAP1 - yeast (Saccharomyce [nrdb_11152004_Chlamydomonas]
  • SAP1 SGDID:S000000849, Chr V from 246502-243809, reverse complement, Verified ORF, "Putative ATPase of the AAA family, interacts with the Sin1p transcriptional repressor in the two-hybrid system" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta]
  • Yer047cp [Saccharomyces cerevisiae] [nr-271106-contam.fasta]
  • Putative ATPase of the AAA family, interacts with the Sin1p transcriptional repressor in the two-hybrid system; Sap1p [Saccharomyces cerevisiae] [nr-271106-contam.fasta]
  • Protein SAP1 [nr-271106-contam.fasta]
  • [chemostatdb.fasta]
  • Protein SAP1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=SAP1 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta]
  • SAP1 SGDID:S000000849, Chr V from 246503-243810, Genome Release 64-1-1, reverse complement, Verified ORF, "Putative ATPase of the AAA family, interacts with the Sin1p transcriptional repressor in the two-hybrid system" [yeast-030211-contam.fasta]
  • SAP1 SGDID:S000000849, Chr V from 246503-243810, Genome Release 64-2-1, reverse complement, Verified ORF, "Putative ATPase of the AAA family; interacts with the Sin1p transcriptional repressor in the two-hybrid system" [20141208_orf_trans.fasta]
  • YER047C SGDID:S000000849, Chr V from 246503-243810, Genome Release 64-1-1, reverse complement, Verified ORF, "Putative ATPase of the AAA family, interacts with the Sin1p transcriptional repressor in the two-hybrid system" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
    • Organism: Saccharomyces cerevisiae
    Length: 897 amino acids

    Gene Ontology:
    Cellular Component: cytoplasm [IDALink to PubMed article]
    Biological Process: biological_process [ND]
    Molecular Function: ATPase activity [ISSLink to PubMed article]


    Sequence:
    Sequence:
    [PDR BLAST]
    [ProtParam] 
          1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MDSQRSHHIL TRLTKIRRRP QQPLTDFTEL YSRIANETIY YLNLEEKKRY KEALQGWKAL  60
   61 TTDVLFKQTL IEHNYPNTQS YTKDEVSLQN GIRELYHKSV MHLKRVKKLV REEPAPRNDM 120
  121 PSSKTYTNHS SSFTRSTEPP PVFQMVPGRM MKTLRNRNAC GYKTAYSNPS LSSYGNSTSI 180
  181 KRGEDAENIR VNFVPSKPLS NNASRQHKNP IEHNDPPLKK ETELYSDKYI SEPILIDLTN 240
  241 DEDDHDVGIL KGHNVFDEEE SDGFEFDVSD YYDNFSEVDV EEEEEEKEER RRIKTLEAIQ 300
  301 QQMSDLSVTS STSSNKSVSS SENVPGSCIQ SLPTTAPALP SLPPPPLLNV DRASSTGALK 360
  361 PHSLETSTTM DSSKIRNPQI SKLMKNNHVP YLKGTKSTPT LITKSTPTFI TRSKSNTKPI 420
  421 IKSNASSPTS SLTVPNSVIQ KPKTAAMAAK RVLNSKKVAS NPALNTTKKS HPILKSKTAK 480
  481 VPNSSSKKTS SHPSRPVSNS KPYSHGASQN KKPSKNQTTS MSKTNRKIPA QKKIGSPKIE 540
  541 DVGTEDATEH ATSLNEQREE PEIDKKVLRE ILEDEIIDSL QGVDRQAAKQ IFAEIVVHGD 600
  601 EVHWDDIAGL ESAKYSLKEA VVYPFLRPDL FRGLREPVRG MLLFGPPGTG KTMLARAVAT 660
  661 ESHSTFFSIS ASSLTSKYLG ESEKLVRALF AIAKKLSPSI IFVDEIDSIM GSRNNENENE 720
  721 SSRRIKNEFL VQWSSLSSAA AGSNKSNTNN SDTNGDEDDT RVLVLAATNL PWSIDEAARR 780
  781 RFVRRQYIPL PEDQTRHVQF KKLLSHQKHT LTESDFDELV KITEGYSGSD ITSLAKDAAM 840
  841 GPLRDLGDKL LETEREMIRP IGLVDFKNSL VYIKPSVSQD GLVKYEKWAS QFGSSGS

    Protein Complex Data

      PUBLICATION TOPOLOGY COCOMPLEXED PROTEINS
    View Details Krogan NJ, et al. (2006) APC11 SAP1
    View Details Ho Y, et al. (2002) GND1 GND2 NAN1 SAP1

    Mass Spectrometry Data

    The following runs contain data for this protein:

      BAIT COMMENTS PUBLICATION
    View Run CEP3 sample cbf3 from feb 2005 Sandall S, et all (2006)
    View Run RPA135 No Comments Schneider, DA, et al. (2006)
    View Run MLP2 #03 Alpha Factor Prep1-TiO2 Flowthrough Keck JM, et al. (2011)
    View Run MLP2 #12 Mitotic Prep1-TiO2 Flowthrough Keck JM, et al. (2011)
    View Run MLP2 #30 Asynchronous Prep6-TiO2 Phosphopeptide enrichment Keck JM, et al. (2011)

    Yeast Two-Hybrid Data

    The following interactions contain this protein:

    NOT SHOWING SINGLE HITS. [ Show Single Hits ]

    [View our yeast two-hybrid interpretation guidelines.]

      BAIT PREY HITS PUBLICATION
    View Screen PCF11 SAP1 2 Unpublished Fields Lab Data
    View Screen RAD51 SAP1 2 Uetz P, et al. (2000)
    View Screen GTS1 SAP1 2 Unpublished Fields Lab Data
    View Screen SMC5 SAP1 2 Hazbun TR, et al. (2003)
    View Screen BEM4 SAP1 2 Unpublished Fields Lab Data
    View Screen STE50 SAP1 2 Unpublished Fields Lab Data

    Microscopy / Localization Data

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      PROTEIN(S) PUBLICATION
    View Data SAP1 Huh WK, et al. (2003)

    Protein Structure Data


    [What does the above image mean?]
    [View Top Sequence Alignments] [Show Ginzu Version Information]

    Domains predicted:

    #   Region(s) Method Confidence Match Description
    1 View Details [1..157] deduced N/A No confident structure predictions are available.
    2 View Details [158..323] MSA 1.034991 View MSA. No confident structure predictions are available.
    3 View Details [324..404] deduced N/A No confident structure predictions are available.
    4 View Details [405..526] PSI-BLAST 438.616428 Membrane fusion atpase p97 N-terminal domain , P97-Nn; Membrane fusion atpase p97, D1 domain; Membrane fusion atpase p97 domain 2, P97-Nc
    5 View Details [527..601] PSI-BLAST 438.616428 Membrane fusion atpase p97 N-terminal domain , P97-Nn; Membrane fusion atpase p97, D1 domain; Membrane fusion atpase p97 domain 2, P97-Nc
    6 View Details [602..790] PSI-BLAST 438.616428 Membrane fusion atpase p97 N-terminal domain , P97-Nn; Membrane fusion atpase p97, D1 domain; Membrane fusion atpase p97 domain 2, P97-Nc
    7 View Details [791..897] PSI-BLAST 438.616428 Membrane fusion atpase p97 N-terminal domain , P97-Nn; Membrane fusion atpase p97, D1 domain; Membrane fusion atpase p97 domain 2, P97-Nc


    Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076


    Philius Transmembrane Prediction:

    Protein predicted to be: GLOBULAR (No transmembrane regions or signal peptide)
    Confidence of classification: 0.99

    Source: Reynolds et al. (2008)

    YRC Informatics Platform - Version 3.0
    Created and Maintained by: Michael Riffle