Protein Overview: HSP78

General Information:
Name(s) found:	HSP78 / YDR258C [SGD]
Description(s) found: Found 30 descriptions. SHOW ALL	Oligomeric mitochondrial matrix chaperone that cooperates with Ssc1p in mitochondrial thermotolerance after heat shock; able to prevent the aggregation of misfolded proteins as well as resolubilize protein aggregates [SGD]
Organism:	Saccharomyces cerevisiae
Length:	811 amino acids
Gene Ontology:
Cellular Component:	mitochondrial matrix [IDA] mitochondrion [IDA]
Biological Process:	protein import into mitochondrial matrix [IDA] protein folding [IDA] response to stress [IDA] mitochondrial genome maintenance [IDA]
Molecular Function:	ATPase activity [ISS] unfolded protein binding [IDA]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MLRQATKAPI QKYLQRTQLL RRSTPRIYTI VQCKRSICSF NARPRVANKL LSDIKTNALN  60
   61 EVAISTCALK SSYGLPNFKR TYVQMRMDPN QQPEKPALEQ FGTNLTKLAR DGKLDPVIGR 120
  121 DEEIARAIQI LSRRTKNNPC LIGRAGVGKT ALIDGLAQRI VAGEVPDSLK DKDLVALDLG 180
  181 SLIAGAKYRG EFEERLKKVL EEIDKANGKV IVFIDEVHML LGLGKTDGSM DASNILKPKL 240
  241 ARGLRCISAT TLDEFKIIEK DPALSRRFQP ILLNEPSVSD TISILRGLKE RYEVHHGVRI 300
  301 TDTALVSAAV LSNRYITDRF LPDKAIDLVD EACAVLRLQH ESKPDEIQKL DRAIMKIQIE 360
  361 LESLKKETDP VSVERREALE KDLEMKNDEL NRLTKIWDAE RAEIESIKNA KANLEQARIE 420
  421 LEKCQREGDY TKASELRYSR IPDLEKKVAL SEKSKDGDKV NLLHDSVTSD DISKVVAKMT 480
  481 GIPTETVMKG DKDRLLYMEN SLKERVVGQD EAIAAISDAV RLQRAGLTSE KRPIASFMFL 540
  541 GPTGTGKTEL TKALAEFLFD DESNVIRFDM SEFQEKHTVS RLIGAPPGYV LSESGGQLTE 600
  601 AVRRKPYAVV LFDEFEKAHP DVSKLLLQVL DEGKLTDSLG HHVDFRNTII VMTSNIGQDI 660
  661 LLNDTKLGDD GKIDTATKNK VIEAMKRSYP PEFINRIDDI LVFNRLSKKV LRSIVDIRIA 720
  721 EIQDRLAEKR MKIDLTDEAK DWLTDKGYDQ LYGARPLNRL IHRQILNSMA TFLLKGQIRN 780
  781 GETVRVVVKD TKLVVLPNHE EGEVVEEEAE K

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Qiu et al. (2008)		HSP60 HSP78 MDJ1 MSP1 YME2 ZIM17

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	MLP2	#29 Asynchronous Prep5-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#12 Mitotic Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#02 Alpha Factor Prep1-TiO2 enriched, new search criteria	Keck JM, et al. (2011)
View Run	MLP2	#11 Mitotic Prep1-TiO2 enriched, new search criteria	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	BUR2	HSP78	2	Unpublished Fields Lab Data
View Screen	YHR122W	HSP78	1	Hazbun TR, et al. (2003)
View Screen	RRB1	HSP78	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	HSP78	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..91]	deduced	N/A	Confident ab initio structure predictions are available.
2	View Details	[92..286]	PSI-BLAST	688.124002	ClpB
3	View Details	[287..425]	ORFEUS	8.9	N-terminal, ClpS-binding domain of ClpA, an Hsp100 chaperone; ClpA, an Hsp100 chaperone, AAA+ modules
4	View Details	[426..688]	PSI-BLAST	7.522879	Replication factor C
5	View Details	[689..811]	ORFEUS	12.61	HslU

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
transporter activity	3.04833973586251	bayes_pls_golite062009
hydrogen ion transmembrane transporter activity	2.97325763395103	bayes_pls_golite062009
transmembrane transporter activity	2.88860382281825	bayes_pls_golite062009
monovalent inorganic cation transmembrane transporter activity	2.82839518806942	bayes_pls_golite062009
nucleoside-triphosphatase activity	2.62748401933955	bayes_pls_golite062009
pyrophosphatase activity	2.55282098898104	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides	2.5316131833172	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides	2.53035702378505	bayes_pls_golite062009
binding	2.21604035604783	bayes_pls_golite062009
hydrolase activity	2.18454736627863	bayes_pls_golite062009
hydrogen ion transporting ATP synthase activity, rotational mechanism	2.11085913752215	bayes_pls_golite062009
ATPase activity	2.04651885449197	bayes_pls_golite062009
ATPase activity, coupled	1.87142752786895	bayes_pls_golite062009
substrate-specific transporter activity	1.61003954220885	bayes_pls_golite062009
nucleic acid binding	1.52921795164482	bayes_pls_golite062009
substrate-specific transmembrane transporter activity	1.35976511031033	bayes_pls_golite062009
protein binding	1.22129154759482	bayes_pls_golite062009
DNA binding	1.19966224604123	bayes_pls_golite062009
transcription regulator activity	1.09075553688438	bayes_pls_golite062009
catalytic activity	1.00104173493322	bayes_pls_golite062009
purine nucleotide binding	0.929901285538132	bayes_pls_golite062009
nucleotide binding	0.922442819194247	bayes_pls_golite062009
purine ribonucleotide binding	0.920987930180051	bayes_pls_golite062009
ribonucleotide binding	0.920926158369519	bayes_pls_golite062009
structural constituent of ribosome	0.738234102557832	bayes_pls_golite062009
motor activity	0.70874549510465	bayes_pls_golite062009
guanyl nucleotide binding	0.640772201693186	bayes_pls_golite062009
guanyl ribonucleotide binding	0.63468044350616	bayes_pls_golite062009
DNA-dependent ATPase activity	0.62832939629645	bayes_pls_golite062009
GTP binding	0.621689261700018	bayes_pls_golite062009
ion transmembrane transporter activity	0.613335848236039	bayes_pls_golite062009
GTPase activity	0.437665353870877	bayes_pls_golite062009
active transmembrane transporter activity	0.434775387083003	bayes_pls_golite062009
ATP binding	0.41092997612902	bayes_pls_golite062009
adenyl ribonucleotide binding	0.385813421701492	bayes_pls_golite062009
adenyl nucleotide binding	0.377870145186619	bayes_pls_golite062009
inorganic cation transmembrane transporter activity	0.314819889840159	bayes_pls_golite062009
primary active transmembrane transporter activity	0.294474570607914	bayes_pls_golite062009
translation regulator activity	0.29096092258568	bayes_pls_golite062009
P-P-bond-hydrolysis-driven transmembrane transporter activity	0.282352478532746	bayes_pls_golite062009
translation factor activity, nucleic acid binding	0.274994961137851	bayes_pls_golite062009
cation transmembrane transporter activity	0.226483101618176	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides, catalyzing transmembrane movement of substances	0.220608508617331	bayes_pls_golite062009
ATPase activity, coupled to transmembrane movement of substances	0.219533217661421	bayes_pls_golite062009
ATPase activity, coupled to movement of substances	0.219157471322311	bayes_pls_golite062009
transcription activator activity	0.129948034606198	bayes_pls_golite062009
transcription factor activity	0.0612932659603008	bayes_pls_golite062009
structural molecule activity	0.0558641287316701	bayes_pls_golite062009
structure-specific DNA binding	0.050232896280066	bayes_pls_golite062009
helicase activity	0.0450969969658499	bayes_pls_golite062009
protein transporter activity	0.0071389922757723	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2