Protein Overview: ROX3

General Information:
Name(s) found:	ROX3 / YBL093C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6319377\|ref\|NP_009459.1\| Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; Rox3p [Saccharomyces cerevisiae] [NCBI NR] gi\|45270474\|gb\|AAS56618.1\| YBL093C [Saccharomyces cerevisiae] [NCBI NR] gi\|4364\|emb\|CAA41233.1\| ROX3 nuclear protein [Saccharomyces cerevisiae] [NCBI NR] gi\|2507116\|sp\|P25046.3\|MED19_YEAST RecName: Full=Mediator of RNA polymerase II transcription subunit 19; AltName: Full=Mediator complex subunit 19; AltName: Full=Hypoxic gene repressor protein 3; AltName: Full=Negative regulator of URS2 protein 3; AltName [NCBI NR] gi\|1870089\|emb\|CAA84915.1\| ROX3 [Saccharomyces cerevisiae] [NCBI NR] (P25046) ROX3 nuclear protein [Swiss-Prot] Mediator of RNA polymerase II transcription subunit 19 OS=Saccharomyces cerevisiae GN=ROX3 PE=1 SV=3 [Swiss-Prot] ROX3 Transcription factor [mips] Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme [SGD] ROX3, Chr II from 44250-44912, reverse complement [yeast_orfs2] Chr II from 44250-44912, reverse complement [yeastorf3.fasta] Chr II from 44912-44250, reverse complement [yeastorf5.fasta] ROX3 SGDID:S0000189, Chr II from 44915-44253, reverse complement, Verified ORF [yeastflysequest.fasta] RNA polymerase II holoenzyme component [Saccharomyces cerevisiae]gi\|1870089\|emb\|CAA84915.1\| ROX3 [Saccharomyces cerevisiae]gi\|4364\|emb\|CAA41233.1\| ROX3 nuclear protein [Saccharomyces cerevisiae]gi\|45270474\|gb\|AAS56618.1\| YBL093C [Saccharomyces cerevisi [nrdb_11152004_Chlamydomonas] ROX3 SGDID:S000000189, Chr II from 44915-44253, reverse complement, Verified ORF, "RNA polymerase II holoenzyme component" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] ROX3 SGDID:S000000189, Chr II from 44915-44253, reverse complement, Verified ORF, "Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme" [SGD_S-cerevisiae_na_08-19-2008_con_reversed.fasta] YBL093C [Saccharomyces cerevisiae] [nr-271106-contam.fasta] RNA polymerase II mediator complex subunit 19 (Hypoxic gene repressor protein 3) (Negative regulator of URS2 protein 3) (SNF1 suppressor protein 7) [nr-271106-contam.fasta] RNA polymerase II holoenzyme component; Rox3p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] ROX3 nuclear protein [Saccharomyces cerevisiae] [nr-271106-contam.fasta] ROX3 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] [chemostatdb.fasta] Mediator of RNA polymerase II transcription subunit 19 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=ROX3 PE=1 SV=3 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] ROX3 SGDID:S000000189, Chr II from 44918-44256, Genome Release 64-1-1, reverse complement, Verified ORF, "Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme" [yeast-030211-contam.fasta] ROX3 SGDID:S000000189, Chr II from 44918-44256, Genome Release 64-2-1, reverse complement, Verified ORF, "Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme" [20141208_orf_trans.fasta] TR4346\|c0_g1_i1\|g.3055 ORF TR4346\|c0_g1_i1\|g.3055 TR4346\|c0_g1_i1\|m.3055 type:complete len:221 (+) TR4346\|c0_g1_i1:152-814(+) [Y12_S_cerevisiae-Sep2015-contam.fasta] TR2463\|c0_g1_i1\|g.1726 ORF TR2463\|c0_g1_i1\|g.1726 TR2463\|c0_g1_i1\|m.1726 type:complete len:221 (+) TR2463\|c0_g1_i1:148-810(+) [DBVPG6044_S_cerevisiae-Sep2015-contam.fasta] YBL093C SGDID:S000000189, Chr II from 44918-44256, Genome Release 64-1-1, reverse complement, Verified ORF, "Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta] asmbl_4875\|g.3594 ORF asmbl_4875\|g.3594 asmbl_4875\|m.3594 type:complete len:221 (+) asmbl_4875:174-836(+) [DBVPG6044_Trinity_v2_2_0_PASA_6May2016-contam.fasta] asmbl_4873\|g.3685 ORF asmbl_4873\|g.3685 asmbl_4873\|m.3685 type:complete len:221 (+) asmbl_4873:154-816(+) [Y12_Trinity_v2_2_0_PASA_6May2016-contam.fasta] ID=asmbl_4544\|g.2826 ORF ID=asmbl_4544\|g.2826 ID=asmbl_4544\|m.2826 type:complete len:221 (+) ID=asmbl_4544:174-836(+) [DBVPG6044_quiverGenomes_Trinity_v2_2_0_PASA_2June2016-contam.fasta] ID=asmbl_4877\|g.2768 ORF ID=asmbl_4877\|g.2768 ID=asmbl_4877\|m.2768 type:complete len:221 (+) ID=asmbl_4877:154-816(+) [Y12_quiver_Trinity_v2_2_0_PASA_2June2016-contam.fasta]
Organism:	Saccharomyces cerevisiae
Length:	220 amino acids
Gene Ontology:
Cellular Component:	nucleus [IDA]
Biological Process:	transcription from RNA polymerase II promoter [IDA]
Molecular Function:	RNA polymerase II transcription mediator activity [IDA]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MASRVDETTV PSYYYYVDPE TTYTYQQPNP LQDLISVYGL DDISRQVART NLDGTKAVKL  60
   61 RKSYKNQIAD LSGKFSTIPT RENGKGGQIA HILFQNNPDM MIQPPQQGQN MSEQQWREQL 120
  121 RNRDIALFQP PNFDWDLCSS VLSQFERSYP SEFANQNQGG AQAPFDIDDL AFDLDGTGKS 180
  181 QSGSNSGNNS KKRKNKSSGS SMATPTHSDS HEDMKRRRLE

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		CSE2 GAL11 MED1 MED11 MED2 MED4 MED6 MED7 MED8 NUT2 PGD1 ROX3 SIN4 SOH1 SRB2 SRB4 SRB5 SRB6 SRB7
View Details	(MIPS) Mewes HW, et al. (2004)		CSE2 DMC1 GAL11 MED11 MED2 MED4 MED6 MED7 MED8 NUT1 NUT2 PGD1 RGR1 ROX3 SIN4 SRB2 SRB4 SRB5 SRB6 SRB7 TAF14
View Details	Krogan NJ, et al. (2006)		CSE2 DSS1 GAL11 GPT2 IRC11 MED1 MED11 MED2 MED4 MED6 MED7 MED8 NUT1 NUT2 PGD1 RGR1 ROX3 SIN4 SOH1 SRB2 SRB4 SRB5 SRB6 SRB7 SSN3 SSN8 THI72
View Details	Gavin AC, et al. (2002)		ADA2 ADH1 COG3 CSE2 ENO2 FAB1 FBA1 GAL11 GCN5 MED1 MED11 MED2 MED4 MED6 MED7 MED8 MLP1 MRPL6 NUT1 NUT2 PGD1 PGK1 RGR1 ROX3 RPG1 RPO21 SIN4 SPT7 SRB2 SRB4 SRB5 SRB6 SRB7 SRB8 SRP1 SSE2 SSN2 SSN3 SSN8 TAF6 TIF1, TIF2 YIL077C
View Details	Qiu et al. (2008)		ADA2 CSE2 GAL11 MED1 MED11 MED2 MED4 MED6 MED7 MED8 NUT2 PGD1 RGR1 ROX3 SIN4 SOH1 SPT15 SRB2 SRB4 SRB5 SRB6 SRB7 SSL2 TAF10 TAF12 TAF13 TAF14 TFB5

Mass Spectrometry Data

No mass spectrometry results found for this protein.

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	FAR10	ROX3	2	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	ROX3	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..83]	MSA	1.002997	View MSA. Confident ab initio structure predictions are available.
2	View Details	[84..220]	deduced	N/A	No confident structure predictions are available.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2