Protein Overview: GRH1

General Information:
Name(s) found:	GRH1 / YDR517W [SGD]
Description(s) found: Found 27 descriptions. SHOW ALL	Acetylated, cis-golgi localized protein involved in ER to Golgi transport; homolog of human GRASP65; forms a complex with the coiled-coil protein Bug1p; mutants are compromised for the fusion of ER-derived vesicles with Golgi membranes [SGD]
Organism:	Saccharomyces cerevisiae
Length:	372 amino acids
Gene Ontology:
Cellular Component:	cytoplasm [IDA ]
Biological Process:	mitotic cell cycle spindle assembly checkpoint [IMP] Golgi organization [ISS]
Molecular Function:	molecular_function [ND]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MFRIAKNLVR TFEQSVQDTL ALSQDSSNLD AFFQSIPPNL LSAQLESPVD AVSEGVKHTN  60
   61 VNETLSGLRI VWVDEMQFQL QSFFDYIVGF NDDPVPVVSN QHGFSYPDYR RITSIFNEHC 120
  121 GRTLKVNIWS AKGGTFRDEY ISIISKESDD LDDVSLNHDE RRPSSGEAHQ FQALGFKVQW 180
  181 TPLIASTFTY HILNVNIPDG PAQSAGLIPD EDYIIGCQDG LLATGGETLL QDIVRSRANY 240
  241 DLVLYVYNKV SDCVRPITVH IGPDGRLGCN VGYGFLHRIP TVKHCPQQAQ QQGQDDNPVP 300
  301 VPVPVESETA FVPSAFTAPP VPTKKKSKNK KGTQPLAMDD YFNEGRDKSS TAAKSAESDI 360
  361 LAPPPQKQSS SD

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Krogan NJ, et al. (2006)		BUG1 GRH1 SEC23 SEC24 SFB2
View Details	Ho Y, et al. (2002)		AAC3 ARP4 ASA1 ATG17 ATG29 ATP3 CDC33 CIC1 CIS1 CYS4 FOL2 GRH1 HAP2 HAP5 IPP1 LOC1 MAM33 MTQ1 NAP1 NMD5 NOP16 NSA2 POL5 PSE1 RHR2 SAH1 SAP190 SPE3 SSK2 TIF1, TIF2 TIF6 YRA1 YRA2 YTM1

Mass Spectrometry Data

No mass spectrometry results found for this protein.

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	UBC6	GRH1	2	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	GRH1	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..284]	MSA	3.016953	View MSA. No confident structure predictions are available.
2	View Details	[285..372]	MSA	4.014979	View MSA. No confident structure predictions are available.

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
binding	2.70495852228344	bayes_pls_golite062009
protein binding	2.17099732113956	bayes_pls_golite062009
transporter activity	1.20918305129892	bayes_pls_golite062009
transmembrane transporter activity	1.08101408526167	bayes_pls_golite062009
substrate-specific transporter activity	0.768438717123608	bayes_pls_golite062009
cytoskeletal protein binding	0.717837655351774	bayes_pls_golite062009
substrate-specific transmembrane transporter activity	0.557123581800701	bayes_pls_golite062009
active transmembrane transporter activity	0.260876923856991	bayes_pls_golite062009
protein transporter activity	0.184650447502837	bayes_pls_golite062009
signal transducer activity	0.0148882826722625	bayes_pls_golite062009
molecular transducer activity	0.0148882826722625	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2