Protein Overview: DNApol-alpha180-PA

General Information:
Name(s) found:	DNApol-alpha180-PA / FBpp0083514 [FlyBase]
Description(s) found: Found 20 descriptions. SHOW ALL	The gene DNA polymerase Ă?Ă?Âą 180kD is referred to in FlyBase by the symbol Dmel\DNApol-Ă?Ă?Âą180 (CG6349, FBgn0259113). It is a protein_coding_gene from Drosophila melanogaster. Its sequence location is 3R:17492275..17497472. It has the cytological map location 93F2. There is experimental evidence that it has the molecular function</span>: DNA-directed DNA polymerase activity. There is experimental evidence that it is involved in the biological process</span>: DNA replication. 6 alleles are reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide. [FlyBase]
Organism:	Drosophila melanogaster
Length:	1488 amino acids
Gene Ontology:
Cellular Component:	nucleus [IDA] alpha DNA polymerase:primase complex [IDA][ISS][NAS]
Biological Process:	DNA replication [IDA][IEA] DNA strand elongation involved in DNA replication [NAS] nucleobase, nucleoside, nucleotide and nucleic acid metabolic process [IEA]
Molecular Function:	nucleotide binding [IEA] DNA binding [IEA] DNA-directed DNA polymerase activity [IDA][ISS][NAS] 3'-5'-exodeoxyribonuclease activity [NAS] nucleoside binding [IEA]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSESPSEPRA KRQRVDKNGR FAAMERLRQL KGTKNKCKVE DQVDDVYDVV DEREYAKRAQ  60
   61 EKYGDDWIEE DGTGYAEDLR DFFEDEDEYS DGEEDRKDSK KKKGVAPNSK KRPRENEKPV 120
  121 TGKASIKNLF SNAVPKKMDV KTSVKDDDIL ADILGEIKEE PAATSEKAEK VIAPAKISVT 180
  181 SRKFDAAAAK EYMNSFLNNI KVQEQERKKA EASSDNEMLE RILKPKAAVP NTKVAFFSSP 240
  241 TIKKEPMPEK TPAKKATEDP FSDNEMDFSC LDDDENQFDV EKTQQTEKVS QTKTAAEKTS 300
  301 QSKVAEKSAP KKETTGSPKE SESEDISRLL NNWESICQMD DDFEKSVLTT EQDSTISSDQ 360
  361 QLRFWYWEAY EDPVKMPGEV FLFGRTADGK SVCLRVQNIN RVLYLLPRQF LLDPISKEPT 420
  421 KQKVTVADIY KEFDSEVANQ LKLEFFRSRK VTKSFAHHAI GIEVPQSCDY LEVHYDGKKP 480
  481 LPNLSADKKY NSIAHIFGAT TNALERFLLD RKIKGPCWLQ VTGFKVSPTP MSWCNTEVTL 540
  541 TEPKNVELVQ DKGKPAPPPP LTLLSLNVRT SMNPKTSRNE ICMISMLTHN RFHIDRPAPQ 600
  601 PAFNRHMCAL TRPAVVSWPL DLNFEMAKYK STTVHKHDSE RALLSWFLAQ YQKIDADLIV 660
  661 TFDSMDCQLN VITDQIVALK IPQWSRMGRL RLSQSFGKRL LEHFVGRMVC DVKRSAEECI 720
  721 RARSYDLQTL CKQVLKLKES ERMEVNADDL LEMYEKGESI TKLISLTMQD NSYLLRLMCE 780
  781 LNIMPLALQI TNICGNTMTR TLQGGRSERN EFLLLHAFHE KNYIVPDKKP VSKRSGAGDT 840
  841 DATLSGADAT MQTKKKAAYA GGLVLEPMRG LYEKYVLLMD FNSLYPSIIQ EYNICFTTVQ 900
  901 QPVDADELPT LPDSKTEPGI LPLQLKRLVE SRKEVKKLMA APDLSPELQM QYHIRQMALK 960
  961 LTANSMYGCL GFAHSRFFAQ HLAALVTHKG REILTNTQQL VQKMNYDVVY GDTDSLMINT1020
 1021 NITDYDQVYK IGHNIKQSVN KLYKQLELDI DGVFGCLLLL KKKKYAAIKL SKDSKGNLRR1080
 1081 EQEHKGLDIV RRDWSQLAVM VGKAVLDEVL SEKPLEEKLD AVHAQLEKIK TQIAEGVVPL1140
 1141 PLFVITKQLT RTPQEYANSA SLPHVQVALR MNRERNRRYK KGDMVDYVIC LDGTTNAAMQ1200
 1201 RAYHLDELKT SEDKKLQLDT NYYLGHQIHP VVTRMVEVLE GTDASRIAEC LGMDPTKFRQ1260
 1261 NAQRTQRENT EQSEGESLLK TTLQLYRLCE PFRFQCVTCK TEQLMASAYR PGPSNSHIAV1320
 1321 LQQCAKSECQ TAPIQYLASV RNQLQLSMRQ YVQRFYKNWL VCDHPDCNFN TRTHSLRKKS1380
 1381 HRPLCQKCRS GSLLRQYTER DLYNQLCYLR FMFDLGKQTL QQKPTLTPEL EQAYQLLYET1440
 1441 VDQQLQSSSY VIISLSKLFA RSLAQMSLQP SVAQPQIEAI PSALADVV

Protein Complex Data

No complex found for this protein.

Mass Spectrometry Data

No mass spectrometry results found for this protein.

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

No yeast two-hybrid interactions found for this protein.

Searching the YRC Public Image Repository, found results:

Currently showing to of results.

[View Search in the YRC Public Image Repository]

Searching the YRC Public Image Repository, found no results.

Ginzu Version Information

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

Gene Ontology predictions

No functions predicted.

Term	Confidence	Notes
DNA-directed DNA polymerase activity	11.2781960093187	bayes_pls_golite062009
DNA polymerase activity	11.2285838712952	bayes_pls_golite062009
nucleotidyltransferase activity	8.85826259809475	bayes_pls_golite062009
DNA primase activity	8.57378684936335	bayes_pls_golite062009
transferase activity, transferring phosphorus-containing groups	4.79232184185512	bayes_pls_golite062009
transferase activity	4.02382113115178	bayes_pls_golite062009
RNA polymerase activity	2.29514625858236	bayes_pls_golite062009
DNA-directed RNA polymerase activity	2.29514625858236	bayes_pls_golite062009
ribonuclease activity	2.10426652514018	bayes_pls_golite062009
exonuclease activity	1.68989335095363	bayes_pls_golite062009
nucleic acid binding	1.62213678119562	bayes_pls_golite062009
DNA binding	1.55767764828811	bayes_pls_golite062009
binding	1.23825244840872	bayes_pls_golite062009
damaged DNA binding	1.08077297289305	bayes_pls_golite062009
catalytic activity	1.04899053677564	bayes_pls_golite062009
endonuclease activity	0.991569665925888	bayes_pls_golite062009
transcription regulator activity	0.863544378011356	bayes_pls_golite062009
exoribonuclease activity	0.85780161294346	bayes_pls_golite062009
3'-5' exonuclease activity	0.78558451404229	bayes_pls_golite062009
exoribonuclease activity, producing 5'-phosphomonoesters	0.78013028675591	bayes_pls_golite062009
endodeoxyribonuclease activity	0.48776342704945	bayes_pls_golite062009
structure-specific DNA binding	0.433956339585542	bayes_pls_golite062009
3'-5'-exoribonuclease activity	0.43313823287883	bayes_pls_golite062009
chromatin binding	0.382725239240319	bayes_pls_golite062009
protein binding	0.377121823388117	bayes_pls_golite062009
nuclease activity	0.332024848299731	bayes_pls_golite062009
DNA helicase activity	0.283883977748512	bayes_pls_golite062009
endonuclease activity, active with either ribo- or deoxyribonucleic acids and producing 5'-phosphomonoesters	0.262305398570872	bayes_pls_golite062009
single-stranded DNA binding	0.206750180223289	bayes_pls_golite062009
single-stranded DNA specific exodeoxyribonuclease activity	0.19596408995795	bayes_pls_golite062009
endoribonuclease activity, producing 5'-phosphomonoesters	0.193854882336037	bayes_pls_golite062009
5'-3' exonuclease activity	0.154583332107704	bayes_pls_golite062009
transcription factor activity	0.093362945686819	bayes_pls_golite062009
nucleoside-triphosphatase activity	0.0873722112846369	bayes_pls_golite062009
pyrophosphatase activity	0.0504884604991491	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides	0.0416785534748089	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides	0.039082818442797	bayes_pls_golite062009

No functions predicted.

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..344]	PSI-BLAST	8.154902	Heavy meromyosin subfragment
2	View Details	[345..1253]	PSI-BLAST	153.0	Exonuclease domain of family B (archaeal and phage) DNA polymerases; T4-like DNA polymerase
3	View Details	[1254..1488]	Pfam	25.346787	No description for PF08996.1 was found. No confident structure predictions are available.

Protein Overview: DNApol-alpha180-PA

Protein Complex Data

Mass Spectrometry Data

Yeast Two-Hybrid Data

Microscopy / Localization Data

Protein Structure Data

Philius Transmembrane Prediction: