Protein Overview: PIN3

General Information:
Name(s) found:	PIN3 / YPR154W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|849162\|gb\|AAB68051.1\| Ypr154wp [Saccharomyces cerevisiae] [NCBI NR] gi\|74676395\|sp\|Q06449.1\|PIN3_YEAST RecName: Full=[PSI+] inducibility protein 3; AltName: Full=LAS seventeen-binding protein 2; Short=LAS17-binding protein 2 [NCBI NR] gi\|6325412\|ref\|NP_015480.1\| Protein that induces appearance of [PIN+] prion when overproduced; Pin3p [Saccharomyces cerevisiae] [NCBI NR] gi\|51013429\|gb\|AAT93008.1\| YPR154W [Saccharomyces cerevisiae] [NCBI NR] pir\|\|S61138 hypothetical protein YPR154w - yeast (Saccharomyces cerevisiae) [NCBI NR] [PSI+] inducibility protein 3 OS=Saccharomyces cerevisiae GN=PIN3 PE=1 SV=1 [Swiss-Prot] PIN3 Psi+ INducibility [mips] Protein that induces appearance of [PIN+] prion when overproduced [SGD] >Q06449 (Q06449) Similar to SEVERAL SH3 domain-containing proteins [4932.FASTACCERV.txt] Chr XVI from 834559-835206 [hmr_yeast5_DM3-1_pombe.fasta] Chr XVI from 834560-835207 [yeastorf3.fasta] YPR154W, Chr XVI from 834560-835207 [yeast_orfs2] PIN3 SGDID:S0006358, Chr XVI from 834559-835206, Verified ORF [yeastflysequest.fasta] PIN3 SGDID:S0006358, Chr XVI from 834561-835208, Verified ORF [10112005cervSGD.fasta] Pin3p [Saccharomyces cerevisiae]gi\|849162\|gb\|AAB68051.1\| Ypr154wp [Saccharomyces cerevisiae]gi\|51013429\|gb\|AAT93008.1\| YPR154W [Saccharomyces cerevisiae]gi\|2132308\|pir\|\|S61138 hypothetical protein YPR154w - yeast (Saccharomyces cerevisiae) [nrdb_11152004_Chlamydomonas] (Q06449) Ypr154wp (YPR154W) [cerv4932.FASTAC] PIN3 SGDID:S000006358, Chr XVI from 834561-835208, Verified ORF, "Protein that induces appearance of [PIN+] prion when overproduced" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] Protein that induces appearance of [PIN+] prion when overproduced; Pin3p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Ypr154wp [Saccharomyces cerevisiae] [nr-271106-contam.fasta] YPR154W [Saccharomyces cerevisiae] [nr-271106-contam.fasta] [chemostatdb.fasta] [PSI+] inducibility protein 3 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=PIN3 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] PIN3 SGDID:S000006358, Chr XVI from 834565-835212, Genome Release 64-1-1, Verified ORF, "Protein that induces appearance of [PIN+] prion when overproduced" [yeast-030211-contam.fasta] PIN3 SGDID:S000006358, Chr XVI from 834565-835212, Genome Release 64-2-1, Verified ORF, "Negative regulator of actin nucleation-promoting factor activity; interacts with Las17p, a homolog of human Wiskott-Aldrich Syndrome protein (WASP), via an N-terminal SH3 domain, and along with LSB1 cooperatively inhibits the nucleation of actin filaments; short-lived protein whose levels increase in response to thermal stress; induces the formation of the [PIN+] and [RNQ+] prions when overproduced; PIN3 has a paralog, LSB1, that arose from the whole genome duplication" [20141208_orf_trans.fasta] YPR154W SGDID:S000006358, Chr XVI from 834565-835212, Genome Release 64-1-1, Verified ORF, "Protein that induces appearance of [PIN+] prion when overproduced" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	215 amino acids
Gene Ontology:
Cellular Component:	nucleus [IDA] cytoplasm [IDA]
Biological Process:	actin cytoskeleton organization [IPI]
Molecular Function:	molecular_function [ND]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSASLINRSL TNIRTELDFL KGSNVISNDV YDQINKSLPA KWDPANAPRN ASPASLEYVE  60
   61 ALYQFDPQQD GDLGLKPGDK VQLLEKLSPE WYKGSCNGRT GIFPANYVKP AFSGSNGPSN 120
  121 LPPPPQYKAQ ELQQIPTQNS AASSYQQQPF PPPSTNYYQQ PQQQPQQAPP PQQQQQQQQH 180
  181 QSSHSHLKSF GSKLGNAAIF GAGASIGSDI VNNIF

Protein Complex Data

No complex found for this protein.

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	TOR2	No Comments	Reinke A, et al. (2004)
View Run	RPA135	his-HA tag on RPA135	Schneider, DA, et al. (2006)
View Run	RPA135	his-HA tag on RPA135	Schneider, DA, et al. (2006)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	CDC40	PIN3	1	Unpublished Fields Lab Data
View Screen	YDR413C	PIN3	1	Unpublished Fields Lab Data
View Screen	CDC14	PIN3	1	Unpublished Fields Lab Data
View Screen	IRE1	PIN3	1	Unpublished Fields Lab Data
View Screen	SDP1	PIN3	1	Unpublished Fields Lab Data
View Screen	FAR10	PIN3	1	Unpublished Fields Lab Data
View Screen	MPD2	PIN3	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	PIN3	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..122]	PSI-BLAST	161.503332	Growth factor receptor-bound protein 2 (GRB2), N- and C-terminal domains; Growth factor receptor-bound protein 2 (GRB2)
2	View Details	[123..215]	MSA	2.484997	View MSA. No confident structure predictions are available.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.77

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2