Protein Overview: BEM4

General Information:
Name(s) found:	BEM4 / YPL161C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|728960\|sp\|P39011.1\|BEM4_YEAST RecName: Full=Bud emergence protein 4 [NCBI NR] gi\|6325096\|ref\|NP_015164.1\| Protein involved in establishment of cell polarity and bud emergence; interacts with the Rho1p small GTP-binding protein and with the Rho-type GTPase Cdc42p; involved in maintenance of proper telomere length; Bem4p [Saccharomyc [NCBI NR] pir\|\|S45177 BEM4 protein - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|450309\|gb\|AAB47774.1\| Bem4p [Saccharomyces cerevisiae] [NCBI NR] gi\|1403548\|emb\|CAA65560.1\| P2561 protein [Saccharomyces cerevisiae] [NCBI NR] gi\|1370342\|emb\|CAA97866.1\| BEM4 [Saccharomyces cerevisiae] [NCBI NR] Bud emergence protein 4 OS=Saccharomyces cerevisiae GN=BEM4 PE=1 SV=1 [Swiss-Prot] BEM4 Bud emergence protein [mips] Protein involved in establishment of cell polarity and bud emergence; interacts with the Rho1p small GTP-binding protein and with the Rho-type GTPase Cdc42p; involved in maintenance of proper telomere length [SGD] (P39011) BEM4 protein [4932.FASTACCERV.txt] Chr XVI from 246219-244318, reverse complement [hmr_yeast5_DM3-1_pombe.fasta] Chr XVI from 244318-246219, reverse complement [yeastorf3.fasta] BEM4, Chr XVI from 244318-246219, reverse complement [yeast_orfs2] BEM4 SGDID:S0006082, Chr XVI from 246219-244318, reverse complement, Verified ORF [yeastflysequest.fasta] Bem4p [Saccharomyces cerevisiae]gi\|1403548\|emb\|CAA65560.1\| P2561 protein [Saccharomyces cerevisiae]gi\|1370342\|emb\|CAA97866.1\| BEM4 [Saccharomyces cerevisiae]gi\|728960\|sp\|P39011\|BEM4_YEAST BEM4 proteingi\|626106\|pir\|\|S45177 BEM4 protein - yeast (Sacchar [nrdb_11152004_Chlamydomonas] BEM4 SGDID:S000006082, Chr XVI from 246219-244318, reverse complement, Verified ORF, "Protein involved in establishment of cell polarity and bud emergence; interacts with the Rho1p small GTP-binding protein and with the Rho-type GTPase Cdc42p" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] BEM4 SGDID:S000006082, Chr XVI from 246219-244318, reverse complement, Verified ORF, "Protein involved in establishment of cell polarity and bud emergence; interacts with the Rho1p small GTP-binding protein and with the Rho-type GTPase Cdc42p; involved in [yeast-contam.fasta] Protein involved in establishment of cell polarity and bud emergence; interacts with the Rho1p small GTP-binding protein and with the Rho-type GTPase Cdc42p; involved in maintenance of proper telomere length; Bem4p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] P2561 protein [Saccharomyces cerevisiae] [nr-271106-contam.fasta] BEM4 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Bud emergence protein 4 [nr-271106-contam.fasta] Bem4p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] BEM4 SGDID:S000006082, Chr XVI from 246219-244318, reverse complement, Verified ORF, "Protein involved in establishment of cell polarity and bud emergence; interacts with the Rho1p small GTP-binding protein and with the Rho-type GTPase Cdc42p; involved in maintenance of proper telomere length" [yeast-bovrabprots.fasta] [chemostatdb.fasta] Bud emergence protein 4 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=BEM4 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] BEM4 SGDID:S000006082, Chr XVI from 246220-244319, Genome Release 64-1-1, reverse complement, Verified ORF, "Protein involved in establishment of cell polarity and bud emergence; interacts with the Rho1p small GTP-binding protein and with the Rho-type GTPase Cdc42p; involved in maintenance of proper telomere length" [yeast-030211-contam.fasta] BEM4 SGDID:S000006082, Chr XVI from 246220-244319, Genome Release 64-2-1, reverse complement, Verified ORF, "Protein involved in establishment of cell polarity and bud emergence; interacts with the Rho1p small GTP-binding protein and with the Rho-type GTPase Cdc42p; involved in maintenance of proper telomere length" [20141208_orf_trans.fasta] YPL161C SGDID:S000006082, Chr XVI from 246220-244319, Genome Release 64-1-1, reverse complement, Verified ORF, "Protein involved in establishment of cell polarity and bud emergence; interacts with the Rho1p small GTP-binding protein and with the Rho-type GTPase Cdc42p; involved in maintenance of proper telomere length" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	633 amino acids
Gene Ontology:
Cellular Component:	nucleus [IDA] cytoplasm [IDA]
Biological Process:	telomere maintenance [IMP] Rho protein signal transduction [IGI] actin cytoskeleton organization [TAS]
Molecular Function:	molecular_function [ND]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MDYEEILFGL QPILNASSIK DVPMNDVYLG SYLAVMDQLA VSLREPSNRD IVGKTGLLLN  60
   61 LVRVLEQALD ICFHDTSISI NDKIAFYEIS SEVIRCIANA IIDNDDNREI LLDSGGKKLL 120
  121 NYYIGGVLQL DEISSDKSED SLVDKLQMRS VVLLRNFCIG NLKYTENLAP FIRGPLFVLL 180
  181 KTTQYSYLSS PEKVVLGSDL LNDILKVNYS NVQISDLFFL SQYIKKISSN VQNKELQAME 240
  241 DGAVEAYSNT ETQKFAGQGN QEYIEKEEED DEEDVNCELL LNLSTCLETI VAKDETINFT 300
  301 NEEQLVLSMQ KNLILSLVCL ESKTFNNKLI VMRRLISCAG NISANLTNSN KREQSLCIET 360
  361 IKSSASSYAL AAALMILCNS VASKSDAVAL LKLISLSELI QVGSLLQDPL QYQGFLDLLR 420
  421 KLLNLENTMW LDIKDLFTLF QIMRRCHEQT KYYNNLRSLL TNLLNKTLTV LPSSKIHNSI 480
  481 SSDPTIISFI AEHGTLTSCI AMDKLLVSKK ALPKEAITSL WDSIFKFQNL GQAEQLSISD 540
  541 LFHITKTVGI YLKDSSVTAD VNPIENILFK DYIQKLTLIL ETILSFKENK DKGSESCFNN 600
  601 GKFIAGIILN IVKNTKCLTP EEQNLEALAK SFF

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Krogan NJ, et al. (2006)		BEM4 SGE1
View Details	Ho Y, et al. (2002)		BEM4 CDC42 SAN1

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)
View Run	MLP2	#03 Alpha Factor Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	CDC42	BEM4	2	Unpublished Fields Lab Data
View Screen	RHO1	BEM4	2	Unpublished Fields Lab Data
View Screen	RHO2	BEM4	2	Drees BL, et al. (2001)
View Screen	BEM4	UBC9	2	Unpublished Fields Lab Data
View Screen	BEM4	SPR28	2	Drees BL, et al. (2001)
View Screen	BEM4	SAP1	2	Unpublished Fields Lab Data
View Screen	BEM4	YER158C	2	Unpublished Fields Lab Data
View Screen	GIC2	BEM4	2	Unpublished Fields Lab Data
View Screen	RSR1	BEM4	2	Drees BL, et al. (2001)
View Screen	BEM4	CDC12	2	Drees BL, et al. (2001)
View Screen	GIC1	BEM4	2	Drees BL, et al. (2001)
View Screen	BEM4	MCM10	2	Unpublished Fields Lab Data
View Screen	RHO4	BEM4	2	Uetz P, et al. (2000)
View Screen	BEM4	CDC11	2	Drees BL, et al. (2001)
View Screen	RHO4	BEM4	2	Unpublished Fields Lab Data
View Screen	BEM4	ENT4	2	Unpublished Fields Lab Data
View Screen	CDC42	BEM4	2	Uetz P, et al. (2000)
View Screen	BEM4	NFI1	2	Drees BL, et al. (2001)
View Screen	CDC42	BEM4	2	Unpublished Fields Lab Data
View Screen	RHO1	BEM4	2	Uetz P, et al. (2000)

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	BEM4	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..633]	deduced	N/A	No confident structure predictions are available.
2	View Details	[183..264]	deduced	N/A	No confident structure predictions are available.
3	View Details	[265..477]	deduced	N/A	No confident structure predictions are available.
4	View Details	[478..633]	deduced	N/A	No confident structure predictions are available.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.95

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2