Protein Overview: SPP1

General Information:
Name(s) found:	SPP1 / YPL138C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6325119\|ref\|NP_015187.1\| Subunit of COMPASS (Set1C), a complex which methylates histone H3 on lysine 4 and is required in telomeric transcriptional silencing; PHD finger domain protein similar to human CGBP, an unmethylated CpG binding protein; Spp1p [ [NCBI NR] gi\|61216382\|sp\|Q03012.1\|SPP1_YEAST RecName: Full=COMPASS component SPP1; AltName: Full=Complex proteins associated with SET1 protein SPP1; AltName: Full=Set1C component SPP1; AltName: Full=Suppressor of PRP protein 1 [NCBI NR] pir\|\|S69047 hypothetical protein YPL138c - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|1244777\|gb\|AAB68222.1\| Lpi8p [NCBI NR] COMPASS component SPP1 OS=Saccharomyces cerevisiae GN=SPP1 PE=1 SV=1 [Swiss-Prot] SPP1 Subunit of the COMPASS complex, which methylates histone H3 on lysine 4 [mips] Subunit of COMPASS (Set1C), a complex which methylates histone H3 on lysine 4 and is required in telomeric transcriptional silencing; interacts with Orc2p; PHD finger domain protein similar to human CGBP, an unmethylated CpG binding protein [SGD] >Q03012 (Q03012) LPI8P [4932.FASTACCERV.txt] Chr XVI from 292426-291365, reverse complement [hmr_yeast5_DM3-1_pombe.fasta] Chr XVI from 291365-292426, reverse complement [yeastorf3.fasta] YPL138C, Chr XVI from 291365-292426, reverse complement [yeast_orfs2] SPP1 SGDID:S0006059, Chr XVI from 292426-291365, reverse complement, Verified ORF [yeastflysequest.fasta] Spp1p [Saccharomyces cerevisiae]gi\|2132201\|pir\|\|S69047 hypothetical protein YPL138c - yeast (Saccharomyces cerevisiae)gi\|1244777\|gb\|AAB68222.1\| Lpi8p [nrdb_11152004_Chlamydomonas] (Q03012) Ypl138cp [cerv4932.FASTAC] SPP1 SGDID:S000006059, Chr XVI from 292426-291365, reverse complement, Verified ORF, "Subunit of COMPASS (Set1C), a complex which methylates Rad6p ubiquitinated histone H3 on lysine 4 and is required in telomeric transcriptional silencing; PHD finger doma [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] SPP1 SGDID:S000006059, Chr XVI from 292426-291365, reverse complement, Verified ORF, "Subunit of COMPASS (Set1C), a complex which methylates histone H3 on lysine 4 and is required in telomeric transcriptional silencing; PHD finger domain protein similar t [yeast-contam.fasta] SPP1 SGDID:S000006059, Chr XVI from 292426-291365, reverse complement, Verified ORF, "Subunit of COMPASS (Set1C), a complex which methylates histone H3 on lysine 4 and is required in telomeric transcriptional silencing; interacts with Orc2p; PHD finger do [yeast-200209-contam.fasta] Subunit of COMPASS (Set1C), a complex which methylates histone H3 on lysine 4 and is required in telomeric transcriptional silencing; PHD finger domain protein similar to human CGBP, an unmethylated CpG binding protein; Spp1p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] COMPASS component SPP1 (Complex proteins associated with SET1 protein SPP1) (Set1C component SPP1) (Suppressor of PRP protein 1) [nr-271106-contam.fasta] Lpi8p [nr-271106-contam.fasta] SPP1 SGDID:S000006059, Chr XVI from 292426-291365, reverse complement, Verified ORF, "Subunit of COMPASS (Set1C), a complex which methylates histone H3 on lysine 4 and is required in telomeric transcriptional silencing; interacts with Orc2p; PHD finger domain protein similar to human CGBP, an unmethylated CpG binding protein" [yeast-bovrabprots.fasta] SPP1 SGDID:S000006059, Chr XVI from 292426-291365, reverse complement, Verified ORF, "Subunit of COMPASS (Set1C), a complex which methylates histone H3 on lysine 4 and is required in telomeric transcriptional silencing; PHD finger domain protein similar to human CGBP, an unmethylated CpG binding protein" [yeast-contam_custom.fasta] [chemostatdb.fasta] COMPASS component SPP1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=SPP1 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] SPP1 SGDID:S000006059, Chr XVI from 292427-291366, Genome Release 64-1-1, reverse complement, Verified ORF, "Subunit of COMPASS (Set1C), a complex which methylates histone H3 on lysine 4 and is required in telomeric transcriptional silencing; interacts with Orc2p; PHD finger domain protein similar to human CGBP, an unmethylated CpG binding protein" [yeast-030211-contam.fasta] SPP1 SGDID:S000006059, Chr XVI from 292427-291366, Genome Release 64-2-1, reverse complement, Verified ORF, "Subunit of COMPASS (Set1C); a complex which methylates histone H3 on lysine 4 and is required in telomeric transcriptional silencing; interacts with Orc2p; PHD finger domain protein similar to human CGBP, an unmethylated CpG binding protein; relocalizes to the cytosol in response to hypoxia" [20141208_orf_trans.fasta] YPL138C SGDID:S000006059, Chr XVI from 292427-291366, Genome Release 64-1-1, reverse complement, Verified ORF, "Subunit of COMPASS (Set1C), a complex which methylates histone H3 on lysine 4 and is required in telomeric transcriptional silencing; interacts with Orc2p; PHD finger domain protein similar to human CGBP, an unmethylated CpG binding protein" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	353 amino acids
Gene Ontology:
Cellular Component:	Set1C/COMPASS complex [IPI ]
Biological Process:	histone methylation [IDA ][IMP] regulation of transcription, DNA-dependent [IMP] chromatin silencing at telomere [IMP ]
Molecular Function:	histone methyltransferase activity (H3-K4 specific) [IDA ][IMP]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSLPQWCPPH STLKRNPTTG EDVYCICKRP DYGELMVGCD GCDDWFHFTC LHIPEQFKDL  60
   61 VFSFYCPYCQ AGITGKNKDA IINGEGSLPK TLWKRKCRIS DCYKPCLQDS KYCSEEHGRE 120
  121 FVNDIWSRLK TDEDRAVVKK MVEQTGHIDK FKKFGQLDFI DNNIVVKTDD EKEIFDQIVV 180
  181 RDMTLKTLED DLQEVQEISL PLFKKKLELL EVYLGWLDNV YTEMRKLDDD AASHVECGKE 240
  241 DSKGTKRKKK KNSSRSRARK NICGYCSTYE RIPCSVEEFV RDFGSNEEAT KIHEVCTKWK 300
  301 CNRHLDWVST NQEQYLQQID SLESMQERLQ HLIQARKKQL NIQYYEEILR RGL

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		BRE2 SDC1 SET1 SHG1 SPP1 SWD1 SWD2 SWD3
View Details	Krogan NJ, et al. (2006)		BRE2 ERF2 SDC1 SET1 SHG1 SPP1 SWD1 SWD3 YHI9 YLL023C
View Details	Gavin AC, et al. (2006)		BRE2 SDC1 SET1 SPP1 SWD1 SWD3
View Details	Gavin AC, et al. (2002)		BRE2 DOA4 RSM7 SDC1 SET1 SPP1 SSZ1 SWD1 SWD3
View Details	Qiu et al. (2008)		BRE2 SDC1 SET1 SHG1 SPP1 SWD1 SWD3

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	CEP3	sample cbf3 from feb 2005	Sandall S, et all (2006)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	SPT21	SPP1	2	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	SPP1	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..109]	PSI-BLAST	3.0	Nuclear corepressor KAP-1 (TIF-1beta)
2	View Details	[110..301]	PSI-BLAST	16.221849	GCN5
3	View Details	[302..353]	deduced	N/A	No confident structure predictions are available.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2