Protein Overview: TRM12

General Information:
Name(s) found:	TRM12 / YML005W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|854485\|emb\|CAA89947.1\| unknown [Saccharomyces cerevisiae] [NCBI NR] gi\|6323638\|ref\|NP_013709.1\| S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; required for wybutosine formation in phenylalanine-accepting tRNA; Trm12p [Saccharomyces cerevisiae] [NCBI NR] gi\|2497109\|sp\|Q04235.1\|TYW2_YEAST RecName: Full=tRNA wybutosine-synthesizing protein 2; AltName: Full=tRNA-yW-synthesizing protein 2; AltName: Full=tRNA methyltransferase 12 [NCBI NR] pir\|\|S55114 hypothetical protein YML005w - yeast (Saccharomyces cerevisiae) [NCBI NR] Uncharacterized protein YML005W - Saccharomyces cerevisiae (Baker's yeast) [Swiss-Prot] tRNA wybutosine-synthesizing protein 2 OS=Saccharomyces cerevisiae GN=TRM12 PE=1 SV=1 [Swiss-Prot] TRM12 tRNA methyltransferase, required for the formation of the hypermodified nucleoside wybutosine in the phenylalanine-accepting tRNA [mips] S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; required for wybutosine formation in phenylalanine-accepting tRNA [SGD] YML005W, Chr XIII from 260221-261609 [yeast_orfs2] Chr XIII from 260221-261609 [yeastorf3.fasta] (Q04235) Hypothetical 52.7 kDa protein in PDR4-GLO1 intergenic region [4932.FASTACCERV.txt] TRM12 SGDID:S0004464, Chr XIII from 260221-261609, Uncharacterized ORF [yeastflysequest.fasta] Trm12p [Saccharomyces cerevisiae]gi\|854485\|emb\|CAA89947.1\| unknown [Saccharomyces cerevisiae]gi\|2497109\|sp\|Q04235\|YMA5_YEAST Hypothetical 52.7 kDa protein in PDR4-GLO1 intergenic regiongi\|1084923\|pir\|\|S55114 hypothetical protein YML005w - yeast (Saccha [nrdb_11152004_Chlamydomonas] TRM12 SGDID:S000004464, Chr XIII from 260221-261609, Verified ORF, "S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; required for wybutosine formation in phenylalanine-accepting tRNA" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; required for wybutosine formation in phenylalanine-accepting tRNA; Trm12p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Hypothetical 52.7 kDa protein in PDR4-GLO1 intergenic region [nr-271106-contam.fasta] unknown [Saccharomyces cerevisiae] [nr-271106-contam.fasta] [chemostatdb.fasta] tRNA wybutosine-synthesizing protein 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=TRM12 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] TRM12 SGDID:S000004464, Chr XIII from 260221-261609, Genome Release 64-1-1, Verified ORF, "S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; required for wybutosine formation in phenylalanine-accepting tRNA" [yeast-030211-contam.fasta] TRM12 SGDID:S000004464, Chr XIII from 260221-261609, Genome Release 64-2-1, Verified ORF, "S-adenosylmethionine-dependent methyltransferase; required for wybutosine formation in phenylalanine-accepting tRNA; member of the seven beta-strand family" [20141208_orf_trans.fasta] YML005W SGDID:S000004464, Chr XIII from 260221-261609, Genome Release 64-1-1, Verified ORF, "S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; required for wybutosine formation in phenylalanine-accepting tRNA" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	462 amino acids
Gene Ontology:
Cellular Component:	cytoplasm [IDA]
Biological Process:	tRNA methylation [IDA]
Molecular Function:	S-adenosylmethionine-dependent methyltransferase activity [IDA][ISS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSDETMPVEF LVSDKRLLKT IKVKLETNGL FVTPIYSDND NKVIKSSIED LNHPLAVEIN  60
   61 NIAGVKARFH ESGNLERSEG HLKHQSNSIT EFTKSFLKDH GLANDKIFLS HLLDHLPLKY 120
  121 TIYPPVVLFN NSTVRSFNHP IWQKAFQLKL FDPNEYYREL LCFLSPGKPS KGTSLHPNNR 180
  181 LLTHLAINNP ITEADVLRRP FNIQPLYGKL IDDSILDDND NTLWENPSQE QLNSSIWCKV 240
  241 IQNGVTQIWS PVFTMFSRGN IKEKKRVLTT FPDICNNDVV DLYAGIGYFT FSYLTKGART 300
  301 LFAFELNPWS VEGLKRGLKA NGFNKSGNCH VFQESNEMCV QRLTEFLSQN PGFRLRIRHI 360
  361 NLGLLPSSKQ GWPLAIKLIY LQGASLEKVT MHIHENVHID AIEDGSFEKN VIVELDAINE 420
  421 SIALIRNRGI KLQFVRSKLE RIKTFAPDIW HVCVDVDVIV ST

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Krogan NJ, et al. (2006)		PLB3 RPS10B SCM3 SUP35 SUP45 TRM12 VPS3

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	MLP2	#32 Asynchronous Prep-No Phosphopeptide enrichment	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	SCL1	TRM12	1	Unpublished Fields Lab Data
View Screen	SPT23	TRM12	1	Unpublished Fields Lab Data

Microscopy / Localization Data

New Feature: Upload Your Own Microscopy Data

	PROTEIN(S)	PUBLICATION
View Data	TRM12	Huh WK, et al. (2003)

Protein Structure Data

[What does the above image mean?]
[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..226]	deduced	N/A	No confident structure predictions are available.
2	View Details	[227..462]	PSI-BLAST	26.221849	Arginine methyltransferase, HMT1

Functions predicted (by domain):

Gene Ontology predictions

No functions predicted.

Term	Confidence	Notes
methyltransferase activity	4.15445027778357	bayes_pls_golite062009
N-methyltransferase activity	4.07806017452628	bayes_pls_golite062009
transferase activity, transferring one-carbon groups	4.07153733729662	bayes_pls_golite062009
protein methyltransferase activity	3.92746815303714	bayes_pls_golite062009
S-adenosylmethionine-dependent methyltransferase activity	3.39429041855776	bayes_pls_golite062009
RNA methyltransferase activity	3.07428840534971	bayes_pls_golite062009
histone methyltransferase activity	2.99461585647365	bayes_pls_golite062009
transferase activity	2.926540904444	bayes_pls_golite062009
tRNA methyltransferase activity	2.5931020126592	bayes_pls_golite062009
arginine N-methyltransferase activity	2.41876112831625	bayes_pls_golite062009
protein-arginine N-methyltransferase activity	2.41876112831625	bayes_pls_golite062009
rRNA methyltransferase activity	2.29640050660273	bayes_pls_golite062009
DNA-methyltransferase activity	2.18991764495147	bayes_pls_golite062009
histone-arginine N-methyltransferase activity	2.04296107639729	bayes_pls_golite062009
tRNA (cytosine-5-)-methyltransferase activity	1.88889414729709	bayes_pls_golite062009
tRNA (cytosine)-methyltransferase activity	1.88889414729709	bayes_pls_golite062009
protein-arginine omega-N monomethyltransferase activity	1.66871163498737	bayes_pls_golite062009
tRNA (adenine-N1-)-methyltransferase activity	1.29759910099835	bayes_pls_golite062009
tRNA (adenine)-methyltransferase activity	1.29759910099835	bayes_pls_golite062009
catalytic activity	1.25333584501255	bayes_pls_golite062009
histone methyltransferase activity (H4-R3 specific)	1.1103522853299	bayes_pls_golite062009
transcription regulator activity	0.914549843668268	bayes_pls_golite062009
protein-arginine omega-N asymmetric methyltransferase activity	0.89325812193225	bayes_pls_golite062009
DNA binding	0.86027591375666	bayes_pls_golite062009
site-specific DNA-methyltransferase (adenine-specific) activity	0.703178429468566	bayes_pls_golite062009
nucleic acid binding	0.616949327277483	bayes_pls_golite062009
rRNA (adenine) methyltransferase activity	0.533090627724779	bayes_pls_golite062009
rRNA (adenine-N6,N6-)-dimethyltransferase activity	0.455449410561251	bayes_pls_golite062009
RNA binding	0.417524996559564	bayes_pls_golite062009
binding	0.383237930789959	bayes_pls_golite062009
protein-arginine omega-N symmetric methyltransferase activity	0.364369105571521	bayes_pls_golite062009
hydrolase activity	0.33020971648583	bayes_pls_golite062009
tRNA (guanine) methyltransferase activity	0.203168546434167	bayes_pls_golite062009

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2