Protein Overview: CDC8

General Information:
Name(s) found:	CDC8 / YJR057W [SGD]
Description(s) found: Found 58 descriptions. SHOW ALL	Thymidylate and uridylate kinase, functions in de novo biosynthesis of pyrimidine deoxyribonucleotides; converts dTMP to dTDP and dUMP to dUTP; essential for mitotic and meiotic DNA replication; homologous to S. pombe Tmp1p [SGD]
Organism:	Saccharomyces cerevisiae
Length:	216 amino acids
Gene Ontology:
Cellular Component:	nucleus [IDA] cytoplasm [IDA]
Biological Process:	mutagenesis [IMP ] DNA repair [IMP] DNA-dependent DNA replication [IDA ][IMP] dTDP biosynthetic process [IDA] plasmid maintenance [IMP] dUDP biosynthetic process [IDA] dTTP biosynthetic process [IDA]
Molecular Function:	thymidylate kinase activity [IDA ] uridylate kinase activity [IDA]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MMGRGKLILI EGLDRTGKTT QCNILYKKLQ PNCKLLKFPE RSTRIGGLIN EYLTDDSFQL  60
   61 SDQAIHLLFS ANRWEIVDKI KKDLLEGKNI VMDRYVYSGV AYSAAKGTNG MDLDWCLQPD 120
  121 VGLLKPDLTL FLSTQDVDNN AEKSGFGDER YETVKFQEKV KQTFMKLLDK EIRKGDESIT 180
  181 IVDVTNKGIQ EVEALIWQIV EPVLSTHIDH DKFSFF

Protein Complex Data

No complex found for this protein.

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	RPA135	his-HA tag on RPA135	Schneider, DA, et al. (2006)
View Run	RPA135	his-HA tag on RPA135	Schneider, DA, et al. (2006)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	SEC17	CDC8	1	Unpublished Fields Lab Data
View Screen	YJR141W	CDC8	1	Unpublished Fields Lab Data
View Screen	PRR1	CDC8	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	CDC8	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..216]	PSI-BLAST	1257.0	Thymidylate kinase

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
nucleobase, nucleoside, nucleotide kinase activity	3.66392587433723	bayes_pls_golite062009
hydrogen ion transporting ATP synthase activity, rotational mechanism	3.01286198424677	bayes_pls_golite062009
nucleotide kinase activity	2.24094813695083	bayes_pls_golite062009
kinase activity	2.0779386406753	bayes_pls_golite062009
hydrogen ion transmembrane transporter activity	2.06460241490722	bayes_pls_golite062009
binding	2.05253348982328	bayes_pls_golite062009
substrate-specific transporter activity	2.0114051513686	bayes_pls_golite062009
phosphotransferase activity, phosphate group as acceptor	1.96697952975573	bayes_pls_golite062009
substrate-specific transmembrane transporter activity	1.92626167278464	bayes_pls_golite062009
monovalent inorganic cation transmembrane transporter activity	1.9136419736217	bayes_pls_golite062009
ion transmembrane transporter activity	1.6854386921174	bayes_pls_golite062009
inorganic cation transmembrane transporter activity	1.64467072658718	bayes_pls_golite062009
cation transmembrane transporter activity	1.5008821138859	bayes_pls_golite062009
nucleic acid binding	1.49633029954726	bayes_pls_golite062009
pyrophosphatase activity	1.49611313354225	bayes_pls_golite062009
DNA-dependent ATPase activity	1.2155186842031	bayes_pls_golite062009
helicase activity	1.16085745407703	bayes_pls_golite062009
catalytic activity	1.00104173493322	bayes_pls_golite062009
DNA binding	0.967303417236485	bayes_pls_golite062009
transcription regulator activity	0.748437190903548	bayes_pls_golite062009
DNA helicase activity	0.72881469450553	bayes_pls_golite062009
structure-specific DNA binding	0.720649395550746	bayes_pls_golite062009
ATP-dependent helicase activity	0.59513742813869	bayes_pls_golite062009
purine NTP-dependent helicase activity	0.59513742813869	bayes_pls_golite062009
double-stranded DNA binding	0.58862275163252	bayes_pls_golite062009
adenylate kinase activity	0.447752711852745	bayes_pls_golite062009
CTP synthase activity	0.435702031552231	bayes_pls_golite062009
protein-DNA loading ATPase activity	0.3625050107782	bayes_pls_golite062009
DNA clamp loader activity	0.27925703205288	bayes_pls_golite062009
ATP-dependent DNA helicase activity	0.22150712609541	bayes_pls_golite062009
nucleotide binding	0.114848452385726	bayes_pls_golite062009
purine nucleotide binding	0.10713474095071	bayes_pls_golite062009
purine ribonucleotide binding	0.0916519414118148	bayes_pls_golite062009
ribonucleotide binding	0.0915913646217636	bayes_pls_golite062009
DNA polymerase activity	4.95561462330096E-4	bayes_pls_golite062009

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2