Protein Overview: MED6

General Information:
Name(s) found:	MED6 / YHR058C [SGD]
Description(s) found: Found 25 descriptions. SHOW ALL	Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation [SGD]
Organism:	Saccharomyces cerevisiae
Length:	295 amino acids
Gene Ontology:
Cellular Component:	mediator complex [IDA]
Biological Process:	transcription from RNA polymerase II promoter [IDA][TAS]
Molecular Function:	RNA polymerase II transcription mediator activity [IDA][TAS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MNVTPLDELQ WKSPEWIQVF GLRTENVLDY FAESPFFDKT SNNQVIKMQR QFSQLNDPNA  60
   61 AVNMTQNIMT LPDGKNGNLE EEFAYVDPAR RQILFKYPMY MQLEEELMKL DGTEYVLSSV 120
  121 REPDFWVIRK QRRTNNSGVG SAKGPEIIPL QDYYIIGANI YQSPTIFKIV QSRLMSTSYH 180
  181 LNSTLESLYD LIEFQPSQGV HYKVPTDTST TATAATNGNN AGGGSNKSSV RPTGGANMAT 240
  241 VPSTTNVNMT VNTMGTGGQT IDNGTGRTGN GNMGITTEML DKLMVTSIRS TPNYI

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		CSE2 GAL11 MED1 MED11 MED2 MED4 MED6 MED7 MED8 NUT1 NUT2 PGD1 RGR1 ROX3 SIN4 SOH1 SRB2 SRB4 SRB5 SRB6 SRB7 SRB8 SSN2 SSN3 YAP1
View Details	(MIPS) Mewes HW, et al. (2004)		CSE2 DMC1 GAL11 MED11 MED2 MED4 MED6 MED7 MED8 NUT1 NUT2 PGD1 RGR1 ROX3 SIN4 SRB2 SRB4 SRB5 SRB6 SRB7 TAF14
View Details	Krogan NJ, et al. (2006)		CSE2 DSS1 GAL11 GPT2 IRC11 MED1 MED11 MED2 MED4 MED6 MED7 MED8 NUT1 NUT2 PGD1 RGR1 ROX3 SIN4 SOH1 SRB2 SRB4 SRB5 SRB6 SRB7 SSN3 SSN8 THI72
View Details	Gavin AC, et al. (2006)		CSE2 GAL11 MED1 MED11 MED6 MED7 MED8 NUT1 NUT2 PGD1 RGR1 SIN4 SRB2 SRB4 SRB5 SRB6 SRB7 SRB8 SSN2
View Details	Gavin AC, et al. (2002)		ADA2 ADH1 COG3 CSE2 ENO2 FAB1 FBA1 GAL11 GCN5 MED1 MED11 MED2 MED4 MED6 MED7 MED8 MLP1 MRPL6 NUT1 NUT2 PGD1 PGK1 RGR1 ROX3 RPG1 RPO21 SIN4 SPT7 SRB2 SRB4 SRB5 SRB6 SRB7 SRB8 SRP1 SSE2 SSN2 SSN3 SSN8 TAF6 TIF1, TIF2 YIL077C
View Details	Qiu et al. (2008)		CSE2 DMC1 GAL11 MED1 MED11 MED2 MED4 MED6 MED7 MED8 NUT1 NUT2 PGD1 RGR1 ROX3 SGF29 SGF73 SIN4 SOH1 SPT20 SPT3 SRB2 SRB4 SRB5 SRB6 SRB7 TAF10 TAF14 TAF3 TAF8 TFB5

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	BOI1	Sample boi1 with gst from october 2005	McCusker D, et al (2007)
View Run	BOI1	Sample bob1 (2nd set) from october 2005	McCusker D, et al (2007)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	TAM41	MED6	1	Hazbun TR, et al. (2003)
View Screen	NPA3	MED6	1	Unpublished Fields Lab Data
View Screen	MDM1	MED6	1	Unpublished Fields Lab Data
View Screen	TIM50	MED6	1	Hazbun TR, et al. (2003)
View Screen	LSM4	MED6	1	Unpublished Fields Lab Data

Microscopy / Localization Data

New Feature: Upload Your Own Microscopy Data

	PROTEIN(S)	PUBLICATION
View Data	MED6	Huh WK, et al. (2003)

Protein Structure Data

[What does the above image mean?]
[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..232]	MSA	5.009964	View MSA. No confident structure predictions are available.
2	View Details	[233..295]	deduced	N/A	No confident structure predictions are available.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2