Protein Overview: SRB5

General Information:
Name(s) found:	SRB5 / YGR104C [SGD]
Description(s) found: Found 27 descriptions. SHOW ALL	Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; involved in telomere maintenance [SGD]
Organism:	Saccharomyces cerevisiae
Length:	307 amino acids
Gene Ontology:
Cellular Component:	mediator complex [IDA]
Biological Process:	telomere maintenance [IMP ] transcription from RNA polymerase II promoter [IDA]
Molecular Function:	RNA polymerase II transcription mediator activity [IDA]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MVQQLSLFGS IGDDGYDLLI STLTTISGNP PLLYNSLCTV WKPNPSYDVE NVNSRNQLVE  60
   61 PNRIKLSKEV PFSYLIDETM MDKPLNFRIL KSFTNDKIPL NYAMTRNILH NTVPQVTNFN 120
  121 STNEDQNNSK HTEDTVNESR NSDDIIDVDM DASPAPSNES CSPWSLQISD IPAAGNNRSV 180
  181 SMQTIAETII LSSAGKNSSV SSLMNGLGYV FEFQYLTIGV KFFMKHGLIL ELQKIWQIEE 240
  241 AGNSQITSGG FLLKAYINVS RGTDIDRINY TETALMNLKK ELQGYIELSV PDRQSMDSRV 300
  301 AHGNILI

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		CSE2 GAL11 MED1 MED11 MED2 MED4 MED6 MED7 MED8 NUT1 NUT2 PGD1 RGR1 ROX3 SIN4 SOH1 SRB2 SRB4 SRB5 SRB6 SRB7 SRB8 SSN2 SSN3 YAP1
View Details	(MIPS) Mewes HW, et al. (2004)		CSE2 DMC1 GAL11 MED11 MED2 MED4 MED6 MED7 MED8 NUT1 NUT2 PGD1 RGR1 ROX3 SIN4 SRB2 SRB4 SRB5 SRB6 SRB7 TAF14
View Details	Krogan NJ, et al. (2006)		CSE2 DSS1 GAL11 GPT2 IRC11 MED1 MED11 MED2 MED4 MED6 MED7 MED8 NUT1 NUT2 PGD1 RGR1 ROX3 SIN4 SOH1 SRB2 SRB4 SRB5 SRB6 SRB7 SSN3 SSN8 THI72
View Details	Gavin AC, et al. (2006)		CSE2 GAL11 MED1 MED11 MED6 MED7 MED8 NUT1 NUT2 PGD1 RGR1 SIN4 SRB2 SRB4 SRB5 SRB6 SRB7 SRB8 SSN2
View Details	Gavin AC, et al. (2002)		ADA2 ADH1 COG3 CSE2 ENO2 FAB1 FBA1 GAL11 GCN5 MED1 MED11 MED2 MED4 MED6 MED7 MED8 MLP1 MRPL6 NUT1 NUT2 PGD1 PGK1 RGR1 ROX3 RPG1 RPO21 SIN4 SPT7 SRB2 SRB4 SRB5 SRB6 SRB7 SRB8 SRP1 SSE2 SSN2 SSN3 SSN8 TAF6 TIF1, TIF2 YIL077C
View Details	Qiu et al. (2008)		ADA2 CSE2 DMC1 GAL11 HFI1 MED1 MED11 MED2 MED4 MED6 MED7 MED8 NUT1 NUT2 PGD1 RGR1 ROX3 RPB4 RPB9 SGF29 SGF73 SIN4 SOH1 SPT20 SRB2 SRB4 SRB5 SRB6 SRB7 TAF10 TAF14 TAF5 TAF9 TFB5

Mass Spectrometry Data

No mass spectrometry results found for this protein.

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	KAP104	SRB5	1	Unpublished Fields Lab Data
View Screen	YJR056C	SRB5	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	SRB5	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..307]	deduced	N/A	No confident structure predictions are available.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.96

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2