Protein Overview: ALT2

General Information:
Name(s) found:	ALT2 / YDR111C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|747886\|emb\|CAA88665.1\| unknown [Saccharomyces cerevisiae] [NCBI NR] gi\|6320317\|ref\|NP_010396.1\| Putative alanine transaminase (glutamic pyruvic transaminase); Alt2p [Saccharomyces cerevisiae] [NCBI NR] gi\|1703231\|sp\|P52892.1\|ALAT_YEAST RecName: Full=Probable alanine aminotransferase; AltName: Full=Glutamic--pyruvic transaminase; AltName: Full=Glutamate pyruvate transaminase; Short=GPT; AltName: Full=Glutamic--alanine transaminase [NCBI NR] pir\|\|S52677 probable membrane protein YDR111c - yeast (Saccharomyces cerevisiae) [NCBI NR] Probable alanine aminotransferase OS=Saccharomyces cerevisiae GN=ALT2 PE=1 SV=1 [Swiss-Prot] ALT2 Putative alanine transaminase (glutamyc pyruvic transaminase) [mips] Putative alanine transaminase (glutamic pyruvic transaminase) [SGD] YDR111C, Chr IV from 678234-679757, reverse complement [yeast_orfs2] Chr IV from 678234-679757, reverse complement [yeastorf3.fasta] Chr IV from 679757-678234, reverse complement [yeastorf5.fasta] (P52892) Putative alanine aminotransferase (EC 2.6.1.2) (Glutamic--pyruvic transaminase) (GPT) (Glutamic--alanine transaminase) [4932.FASTACCERV.txt] ALT2 SGDID:S0002518, Chr IV from 679758-678235, reverse complement, Uncharacterized ORF [yeastflysequest.fasta] ALT2 SGDID:S0002518, Chr IV from 679759-678236, reverse complement, Uncharacterized ORF [10112005cervSGD.fasta] Alt2p [Saccharomyces cerevisiae]gi\|747886\|emb\|CAA88665.1\| unknown [Saccharomyces cerevisiae]gi\|1077571\|pir\|\|S52677 probable membrane protein YDR111c - yeast (Saccharomyces cerevisiae)gi\|1703231\|sp\|P52892\|ALAT_YEAST Putative alanine aminotransferase (Gl [nrdb_11152004_Chlamydomonas] YDR111C SGDID:S000002518, Chr IV from 679759-678236, reverse complement, Uncharacterized ORF, "Putative alanine transaminase (glutamic pyruvic transaminase)" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] YDR111C SGDID:S000002518, Chr IV from 679761-678238, reverse complement, Uncharacterized ORF, "Putative alanine transaminase (glutamic pyruvic transaminase)" [yeast-contam.fasta] ALT2 SGDID:S000002518, Chr IV from 679762-678239, reverse complement, Uncharacterized ORF, "Putative alanine transaminase (glutamic pyruvic transaminase)" [SGD_S-cerevisiae_na_08-19-2008_con_reversed.fasta] unknown [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Putative alanine aminotransferase (Glutamic--pyruvic transaminase) (GPT) (Glutamic--alanine transaminase) [nr-271106-contam.fasta] Putative alanine transaminase (glutamic pyruvic transaminase); Alt2p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] [chemostatdb.fasta] Probable alanine aminotransferase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=ALT2 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] ALT2 SGDID:S000002518, Chr IV from 679764-678241, Genome Release 64-1-1, reverse complement, Uncharacterized ORF, "Putative alanine transaminase (glutamic pyruvic transaminase)" [yeast-030211-contam.fasta] ALT2 SGDID:S000002518, Chr IV from 679764-678241, Genome Release 64-2-1, reverse complement, Uncharacterized ORF, "Catalytically inactive alanine transaminase; expression is repressed in the presence of alanine and repression is mediated by Nrg1p; ALT2 has a paralog, ALT1, that arose from the whole genome duplication" [20141208_orf_trans.fasta] YDR111C SGDID:S000002518, Chr IV from 679764-678241, Genome Release 64-1-1, reverse complement, Uncharacterized ORF, "Putative alanine transaminase (glutamic pyruvic transaminase)" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	507 amino acids
Gene Ontology:
Cellular Component:	nucleus [IDA] cytoplasm [IDA]
Biological Process:	biological_process [ND]
Molecular Function:	transaminase activity [ISS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MTMTHQQDLK GVFTAKDLDF KPAGKITKKD LNTGVTKAEY AVRGAIPTRA DELKEELKKN  60
   61 PEVLPFDDII NANIGNPQQL DQKPLTFTRQ VLAILEYPEI LRVGHNELAS LNLFSRDALE 120
  121 RAERLLNDIG GSIGAYSHSQ GVPGIRQTVA DFITRRDGGE PATPEDIYLT TGASSAATSL 180
  181 LSLLCKDSQT GLLIPIPQYP LYTASASLFN AQVLPYYLDE ESNWSTNSDE IEKVVQDALK 240
  241 KQIRPSVLIV INPGNPTGAV LSEETIARIC LIAAKYGITI ISDEVYQENI FNDVKFHSMK 300
  301 KVLRKLQHLY PGKFDNVQLA SLHSISKGFM DECGQRGGYM EIIGFSQEIR DALFKLMSIS 360
  361 ICSVVTGQAV VDLMVKPPQP GDESYEQDHD ERLKIFHEMR TRANLLYETF KELEGIECQK 420
  421 PQGAMYLFPR LVLPKKALCE SERLGIEPDE FYCTSLLEST GICTVPGSGF GQRPGTYHVR 480
  481 TTFLAPGTKW IQDWKEFHQD FFSKYRN

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Krogan NJ, et al. (2006)		ALT2 RAD5 RRP1
View Details	Gavin AC, et al. (2002)		ACE2 ALT2 BCK1 CDC11 DRE2 ECM30 ENP2 IML1 MNL1 MTR4 MYO2 RGC1 SEC3 UBP15 YBL044W YJR098C YNL313C

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	SOG2	ALT2	2	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	ALT2	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..81] [402..507]	PSI-BLAST	321.9691	1-aminocyclopropane-1-carboxylate synthase (ACC synthase)
2	View Details	[82..401]	PSI-BLAST	321.9691	1-aminocyclopropane-1-carboxylate synthase (ACC synthase)

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
L-aspartate:2-oxoglutarate aminotransferase activity	5.51961002550688	bayes_pls_golite062009
catalytic activity	2.29878871754893	bayes_pls_golite062009
4-aminobutyrate transaminase activity	1.82501790383768	bayes_pls_golite062009
glycine dehydrogenase (decarboxylating) activity	1.63055151623667	bayes_pls_golite062009
oxidoreductase activity, acting on the CH-NH2 group of donors, disulfide as acceptor	1.63055151623667	bayes_pls_golite062009
transferase activity	1.44093490824786	bayes_pls_golite062009
transferase activity, transferring nitrogenous groups	1.28316822767388	bayes_pls_golite062009
transaminase activity	1.24990480711889	bayes_pls_golite062009
L-alanine:2-oxoglutarate aminotransferase activity	1.06988190956423	bayes_pls_golite062009
alanine-oxo-acid transaminase activity	1.06988190956423	bayes_pls_golite062009
hydrolase activity, acting on acid carbon-carbon bonds, in ketonic substances	0.942545094747784	bayes_pls_golite062009
hydrolase activity, acting on acid carbon-carbon bonds	0.845816961387768	bayes_pls_golite062009
N2-acetyl-L-ornithine:2-oxoglutarate 5-aminotransferase activity	0.753443687983955	bayes_pls_golite062009
binding	0.536634592992536	bayes_pls_golite062009
transferase activity, transferring acyl groups	0.319452731970428	bayes_pls_golite062009
pyridoxal phosphate binding	0.16957228630576	bayes_pls_golite062009
vitamin B6 binding	0.16957228630576	bayes_pls_golite062009
aromatic-amino-acid:2-oxoglutarate aminotransferase activity	0.0916183433120769	bayes_pls_golite062009
kynureninase activity	0.0900983660305177	bayes_pls_golite062009
cystathionine beta-lyase activity	0.0833010353195099	bayes_pls_golite062009
nucleic acid binding	0.013097682076602	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.97

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2