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Protein Overview: NFS1

General Information:
Name(s) found: NFS1 / YCL017C [SGD]
Description(s) found:
Found 26 descriptions.
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  • Cysteine desulfurase involved in iron-sulfur cluster (Fe/S) biogenesis; required for the post-transcriptional thio-modification of mitochondrial and cytoplasmic tRNAs; essential protein located predominantly in mitochondria [SGD]
    • Organism: Saccharomyces cerevisiae
    Length: 497 amino acids

    Gene Ontology:
    Cellular Component: nucleus [IMPLink to PubMed article][ISSLink to PubMed article]
    mitochondrion [IDALink to PubMed articleLink to PubMed article][TASLink to PubMed article]
    Biological Process: cellular iron ion homeostasis [IMPLink to PubMed article]
    iron-sulfur cluster assembly [IDALink to PubMed article][ISSLink to PubMed article][TASLink to PubMed article]
    Molecular Function: tRNA sulfurtransferase activity [IMPLink to PubMed article]
    cysteine desulfurase activity [IDALink to PubMed article]
    cystathionine gamma-lyase activity [ISSLink to PubMed article]


    Sequence:
    Sequence:
    [PDR BLAST]
    [ProtParam] 
          1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MLKSTATRSI TRLSQVYNVP AATYRACLVS RRFYSPPAAG VKLDDNFSLE THTDIQAAAK  60
   61 AQASARASAS GTTPDAVVAS GSTAMSHAYQ ENTGFGTRPI YLDMQATTPT DPRVLDTMLK 120
  121 FYTGLYGNPH SNTHSYGWET NTAVENARAH VAKMINADPK EIIFTSGATE SNNMVLKGVP 180
  181 RFYKKTKKHI ITTRTEHKCV LEAARAMMKE GFEVTFLNVD DQGLIDLKEL EDAIRPDTCL 240
  241 VSVMAVNNEI GVIQPIKEIG AICRKNKIYF HTDAAQAYGK IHIDVNEMNI DLLSISSHKI 300
  301 YGPKGIGAIY VRRRPRVRLE PLLSGGGQER GLRSGTLAPP LVAGFGEAAR LMKKEFDNDQ 360
  361 AHIKRLSDKL VKGLLSAEHT TLNGSPDHRY PGCVNVSFAY VEGESLLMAL RDIALSSGSA 420
  421 CTSASLEPSY VLHALGKDDA LAHSSIRFGI GRFSTEEEVD YVVKAVSDRV KFLRELSPLW 480
  481 EMVQEGIDLN SIKWSGH

    Protein Complex Data

      PUBLICATION TOPOLOGY COCOMPLEXED PROTEINS
    View Details Krogan NJ, et al. (2006) ACP1 FMC1 ISD11 NFS1
    View Details Gavin AC, et al. (2006) ISD11 NFS1 TDH3
    View Details Ho Y, et al. (2002) ISU1 NFS1

    Mass Spectrometry Data

    The following runs contain data for this protein:

      BAIT COMMENTS PUBLICATION
    View Run ASF1 3906: TAP-tagged Green EM, et al (2005)

    Yeast Two-Hybrid Data

    The following interactions contain this protein:

    SHOWING SINGLE HITS. [ Hide Single Hits ]

    [View our yeast two-hybrid interpretation guidelines.]

      BAIT PREY HITS PUBLICATION
    View Screen SRO77 NFS1 2 Unpublished Fields Lab Data
    View Screen SWI5 NFS1 1 Unpublished Fields Lab Data
    View Screen PRP42 NFS1 1 Unpublished Fields Lab Data
    View Screen PRE1 NFS1 1 Unpublished Fields Lab Data
    View Screen HSP12 NFS1 1 Unpublished Fields Lab Data
    View Screen YHR022C NFS1 1 Unpublished Fields Lab Data
    View Screen NAF1 NFS1 1 Hazbun TR, et al. (2003)
    View Screen APC5 NFS1 1 Unpublished Fields Lab Data

    Microscopy / Localization Data

    New Feature: Upload Your Own Microscopy Data

      PROTEIN(S) PUBLICATION
    View Data NFS1 Huh WK, et al. (2003)

    Protein Structure Data


    [What does the above image mean?]
    [View Top Sequence Alignments] [Show Ginzu Version Information]

    Domains predicted:

    #   Region(s) Method Confidence Match Description
    1 View Details [1..70] deduced N/A No confident structure predictions are available.
    2 View Details [71..104]
    [358..497]    		 PSI-BLAST 860.9897 NifS-like protein/selenocysteine lyase
    3 View Details [105..357] PSI-BLAST 860.9897 NifS-like protein/selenocysteine lyase

    Functions predicted (by domain):

    # Gene Ontology predictions
    1 No functions predicted.
    2
    Term Confidence Notes
  • oxidoreductase activity, acting on the CH-NH2 group of donors, disulfide as acceptor
    		•  3.40354781002256 bayes_pls_golite062009
  • glycine dehydrogenase (decarboxylating) activity
    		•  3.40354781002256 bayes_pls_golite062009
  • transferase activity
    		•  2.59075614411581 bayes_pls_golite062009
  • L-aspartate:2-oxoglutarate aminotransferase activity
    		•  2.30133284041702 bayes_pls_golite062009
  • catalytic activity
    		•  2.29878871754893 bayes_pls_golite062009
  • transcription regulator activity
    		•  1.67701710033155 bayes_pls_golite062009
  • DNA binding
    		•  1.4774443071095 bayes_pls_golite062009
  • nucleic acid binding
    		•  1.39741122443159 bayes_pls_golite062009
  • cysteine desulfurase activity
    		•  1.30923778688541 bayes_pls_golite062009
  • transferase activity, transferring nitrogenous groups
    		•  1.28316822767388 bayes_pls_golite062009
  • transaminase activity
    		•  1.24990480711889 bayes_pls_golite062009
  • transcription factor activity
    		•  1.15775909410582 bayes_pls_golite062009
  • binding
    		•  1.09053123792737 bayes_pls_golite062009
  • L-alanine:2-oxoglutarate aminotransferase activity
    		•  1.06988190956423 bayes_pls_golite062009
  • alanine-oxo-acid transaminase activity
    		•  1.06988190956423 bayes_pls_golite062009
  • ligase activity
    		•  0.827063849687833 bayes_pls_golite062009
  • structural molecule activity
    		•  0.754675308623467 bayes_pls_golite062009
  • carbon-sulfur lyase activity
    		•  0.698312655247044 bayes_pls_golite062009
  • 5-aminolevulinate synthase activity
    		•  0.54326874956263 bayes_pls_golite062009
  • cystathionine gamma-lyase activity
    		•  0.505859361763689 bayes_pls_golite062009
  • transferase activity, transferring acyl groups
    		•  0.50269055590758 bayes_pls_golite062009
  • cystathionine beta-lyase activity
    		•  0.27933426619091 bayes_pls_golite062009
  • glycine hydroxymethyltransferase activity
    		•  0.271623203801431 bayes_pls_golite062009
  • O-phospho-L-serine:2-oxoglutarate aminotransferase activity
    		•  0.234399054481664 bayes_pls_golite062009
  • RNA binding
    		•  0.222777224430863 bayes_pls_golite062009
  • vitamin B6 binding
    		•  0.16957228630576 bayes_pls_golite062009
  • pyridoxal phosphate binding
    		•  0.16957228630576 bayes_pls_golite062009
  • methyltransferase activity
    		•  0.103986924428507 bayes_pls_golite062009
  • sulfurtransferase activity
    		•  0.0450490929307473 bayes_pls_golite062009
  • transferase activity, transferring one-carbon groups
    		•  0.0254276656964899 bayes_pls_golite062009
    3 No functions predicted.


    Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076


    Philius Transmembrane Prediction:

    Protein predicted to be: GLOBULAR (No transmembrane regions or signal peptide)
    Confidence of classification: 0.97

    Source: Reynolds et al. (2008)

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