YRC Public Data Repository - Protein Structure Prediction

Protein:	NFS1
Organism:	Saccharomyces cerevisiae
Length:	497 amino acids
Reference:	Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for NFS1.

Description	E-value	Query Range	Subject Range
NFS1 - Cysteine desulfurase involved in iron-sulfur cluster (Fe/S) biogenesis; required for the post-transc... NFS1_YEAST - Cysteine desulfurase, mitochondrial OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=NFS1... gi\|151943808 - gi\|151943808\|gb\|EDN62108.1\| nifs-like protein [Saccharomyces cerevisiae YJM789]	0.0	[1..497]	[1..497]
NFS1_ASHGO - Cysteine desulfurase, mitochondrial OS=Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 /... NFS1_ASHGO - Cysteine desulfurase, mitochondrial OS=Ashbya gossypii GN=NFS1 PE=3 SV=1	0.0	[1..497]	[1..490]
gi\|12516934, gi\|... - gi\|15803057\|ref\|NP_289087.1\| cysteine desulfurase [Escherichia coli O157:H7 EDL933], gi\|12516934\|gb\|... gi\|13362867 - gi\|13362867\|dbj\|BAB36819.1\| cysteine desulfurase [Escherichia coli O157:H7 str. Sakai] gi\|73856533, gi\|... - gi\|74313056\|ref\|YP_311475.1\| putative aminotransferase [Shigella sonnei Ss046], gi\|73856533\|gb\|AAZ89... gi\|91211857, gi\|... - gi\|91211857\|ref\|YP_541843.1\| cysteine desulfurase [Escherichia coli UTI89], gi\|91073431\|gb\|ABE08312.... gi\|115513882, gi... - gi\|117624758\|ref\|YP_853671.1\| cysteine desulfurase [Escherichia coli APEC O1], gi\|115513882\|gb\|ABJ01... gi\|237705041, gi... - gi\|237705041\|ref\|ZP_04535522.1\| cysteine desulfurase [Escherichia sp. 3_2_53FAA], gi\|226901407\|gb\|EE... gi\|7447233, gi\|2... - gi\|7447233\|pir\|\|A65030 probable iron-sulfur cofactor synthesis protein b2530 - Escherichia coli (str... gi\|26248898, gi\|... - gi\|26248898\|ref\|NP_754938.1\| cysteine desulfurase [Escherichia coli CFT073], gi\|26109304\|gb\|AAN81506... gi\|82777913, gi\|... - gi\|82777913\|ref\|YP_404262.1\| putative aminotransferase [Shigella dysenteriae Sd197], gi\|81242061\|gb\|... gi\|13362867 - gi\|13362867\|dbj\|BAB36819.1\| cysteine desulfurase [Escherichia coli O157:H7] gi\|82544979, gi\|... - gi\|82544979\|ref\|YP_408926.1\| putative aminotransferase [Shigella boydii Sb227], gi\|81246390\|gb\|ABB67...	0.0	[93..497]	[6..412]
ISCS_PASMU - Cysteine desulfurase OS=Pasteurella multocida GN=iscS PE=3 SV=1 ISCS_PASMU - Cysteine desulfurase IscS OS=Pasteurella multocida (strain Pm70) GN=iscS PE=3 SV=1	0.0	[99..497]	[4..404]
ISCS_SALPB - Cysteine desulfurase IscS OS=Salmonella paratyphi B (strain ATCC BAA-1250 / SPB7) GN=iscS PE=3 SV=1 ISCS_SALEP - Cysteine desulfurase IscS OS=Salmonella enteritidis PT4 (strain P125109) GN=iscS PE=3 SV=1 ISCS_SALG2 - Cysteine desulfurase IscS OS=Salmonella gallinarum (strain 287/91 / NCTC 13346) GN=iscS PE=3 SV=1 gi\|204929449, gi... - gi\|204929449\|ref\|ZP_03220523.1\| cysteine desulfurase IscS [Salmonella enterica subsp. enterica serov... gi\|200389006, gi... - gi\|200389006\|ref\|ZP_03215618.1\| cysteine desulfurase IscS [Salmonella enterica subsp. enterica serov... ISCS_SALTY - Cysteine desulfurase OS=Salmonella typhimurium GN=iscS PE=3 SV=1 ISCS_SALCH - Cysteine desulfurase OS=Salmonella choleraesuis GN=iscS PE=3 SV=1 gi\|238912664 - gi\|238912664\|ref\|ZP_04656501.1\| cysteine desulfurase [Salmonella enterica subsp. enterica serovar Te... ISCS_SALTY - Cysteine desulfurase OS=Salmonella typhimurium GN=iscS PE=3 SV=1 ISCS_SALPA - Cysteine desulfurase IscS OS=Salmonella paratyphi A (strain ATCC 9150 / SARB42) GN=iscS PE=3 SV=1 lcl\|NC_016856.1_... - [gene=nifS] [protein=cysteine desulfurase] [protein_id=YP_005238645.1] [location=complement(2733089.... ISCS_SALPA - Cysteine desulfurase OS=Salmonella paratyphi A GN=iscS PE=3 SV=1 ISCS_SALCH - Cysteine desulfurase OS=Salmonella choleraesuis GN=iscS PE=3 SV=1 ISCS_SALCH - Cysteine desulfurase IscS OS=Salmonella choleraesuis (strain SC-B67) GN=iscS PE=3 SV=1 gi\|194471395, gi... - gi\|194471395\|ref\|ZP_03077379.1\| cysteine desulfurase IscS [Salmonella enterica subsp. enterica serov... ISCS_SALHS - Cysteine desulfurase IscS OS=Salmonella heidelberg (strain SL476) GN=iscS PE=3 SV=1 ISCS_SALNS - Cysteine desulfurase IscS OS=Salmonella newport (strain SL254) GN=iscS PE=3 SV=1 ISCS_SALTY - Cysteine desulfurase IscS OS=Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) GN=iscS PE... gi\|168821477, gi... - gi\|205341918\|gb\|EDZ28682.1\| cysteine desulfurase IscS [Salmonella enterica subsp. enterica serovar W... ISCS_SALDC - Cysteine desulfurase IscS OS=Salmonella dublin (strain CT_02021853) GN=iscS PE=3 SV=1 ISCS_SALPK - Cysteine desulfurase IscS OS=Salmonella paratyphi A (strain AKU_12601) GN=iscS PE=3 SV=1 gi\|197263596, gi... - gi\|197263596\|ref\|ZP_03163670.1\| cysteine desulfurase IscS [Salmonella enterica subsp. enterica serov... ISCS_SALPB - Cysteine desulfurase OS=Salmonella paratyphi B (strain ATCC BAA-1250 / SPB7) GN=iscS PE=3 SV=1 ISCS_SALA4 - Cysteine desulfurase IscS OS=Salmonella agona (strain SL483) GN=iscS PE=3 SV=1 gi\|167992668, gi... - gi\|205329088\|gb\|EDZ15852.1\| cysteine desulfurase IscS [Salmonella enterica subsp. enterica serovar 4... gi\|205325222, gi... - gi\|205325222\|gb\|EDZ13061.1\| cysteine desulfurase IscS [Salmonella enterica subsp. enterica serovar S... gi\|168466701, gi... - gi\|195630769\|gb\|EDX49361.1\| cysteine desulfurase IscS [Salmonella enterica subsp. enterica serovar N... gi\|168261415, gi... - gi\|205349590\|gb\|EDZ36221.1\| cysteine desulfurase IscS [Salmonella enterica subsp. enterica serovar H... gi\|168243327, gi... - gi\|205337629\|gb\|EDZ24393.1\| cysteine desulfurase IscS [Salmonella enterica subsp. enterica serovar H... gi\|205333600, gi... - gi\|205333600\|gb\|EDZ20364.1\| cysteine desulfurase IscS [Salmonella enterica subsp. enterica serovar K...	0.0	[99..497]	[4..404]
gi\|213580753 - gi\|213580753\|ref\|ZP_03362579.1\| cysteine desulfurase [Salmonella enterica subsp. enterica serovar Ty... ISCS_SALTI - Cysteine desulfurase IscS OS=Salmonella typhi GN=iscS PE=3 SV=1 gi\|29136471, gi\|... - gi\|29140846\|ref\|NP_804188.1\| cysteine desulfurase [Salmonella enterica subsp. enterica serovar Typhi... gi\|214000603 - gi\|214000603\|ref\|ZP_03411338.1\| cysteine desulfurase [Salmonella enterica subsp. enterica serovar Ty... gi\|213646425 - gi\|213646425\|ref\|ZP_03376478.1\| cysteine desulfurase [Salmonella enterica subsp. enterica serovar Ty... ISCS_SALTI - Cysteine desulfurase OS=Salmonella typhi GN=iscS PE=3 SV=1 gi\|16761457 - gi\|16761457\|ref\|NP_457074.1\| cysteine desulfurase [Salmonella enterica subsp. enterica serovar Typhi...	0.0	[99..497]	[4..404]
NFS1_CANAL - Cysteine desulfurase, mitochondrial OS=Candida albicans GN=NFS1 PE=3 SV=1 NFS1_CANAL - Cysteine desulfurase, mitochondrial OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=NFS1 PE=2...	0.0	[42..497]	[24..488]
NFS1_CANMA - Cysteine desulfurase, mitochondrial OS=Candida maltosa GN=SPL1 PE=3 SV=1	0.0	[46..497]	[30..484]
NFS1 - NFS1 nitrogen fixation 1 homolog (S. cerevisiae)	0.0	[41..497]	[8..457]

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Predicted Domain #1

Region A:
Residues: [1-70]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MLKSTATRSI TRLSQVYNVP AATYRACLVS RRFYSPPAAG VKLDDNFSLE THTDIQAAAK  60
   61 AQASARASAS

[Run NCBI BLAST on this sequence.]

Detection Method:

deduced

Shown below is our most confident de novo (Rosetta) prediction for this domain.
Click here to view all matches.

Found no confident structure predictions for this domain.

Predicted Domain #2

Region A:
Residues: [71-104]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 GTTPDAVVAS GSTAMSHAYQ ENTGFGTRPI YLDM

[Run NCBI BLAST on this sequence.]

Region B:
Residues: [358-497]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 NDQAHIKRLS DKLVKGLLSA EHTTLNGSPD HRYPGCVNVS FAYVEGESLL MALRDIALSS  60
   61 GSACTSASLE PSYVLHALGK DDALAHSSIR FGIGRFSTEE EVDYVVKAVS DRVKFLRELS 120
  121 PLWEMVQEGI DLNSIKWSGH

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	860.9897
Match:	1ecxA_
Description:	NifS-like protein/selenocysteine lyase
Matching Structure (courtesy of the PDB):

Predicted functions:

Term	Confidence	Notes
oxidoreductase activity, acting on the CH-NH2 group of donors, disulfide as acceptor	3.40354781002256	bayes_pls_golite062009
glycine dehydrogenase (decarboxylating) activity	3.40354781002256	bayes_pls_golite062009
transferase activity	2.59075614411581	bayes_pls_golite062009
L-aspartate:2-oxoglutarate aminotransferase activity	2.30133284041702	bayes_pls_golite062009
catalytic activity	2.29878871754893	bayes_pls_golite062009
transcription regulator activity	1.67701710033155	bayes_pls_golite062009
DNA binding	1.4774443071095	bayes_pls_golite062009
nucleic acid binding	1.39741122443159	bayes_pls_golite062009
cysteine desulfurase activity	1.30923778688541	bayes_pls_golite062009
transferase activity, transferring nitrogenous groups	1.28316822767388	bayes_pls_golite062009
transaminase activity	1.24990480711889	bayes_pls_golite062009
transcription factor activity	1.15775909410582	bayes_pls_golite062009
binding	1.09053123792737	bayes_pls_golite062009
L-alanine:2-oxoglutarate aminotransferase activity	1.06988190956423	bayes_pls_golite062009
alanine-oxo-acid transaminase activity	1.06988190956423	bayes_pls_golite062009
ligase activity	0.827063849687833	bayes_pls_golite062009
structural molecule activity	0.754675308623467	bayes_pls_golite062009
carbon-sulfur lyase activity	0.698312655247044	bayes_pls_golite062009
5-aminolevulinate synthase activity	0.54326874956263	bayes_pls_golite062009
cystathionine gamma-lyase activity	0.505859361763689	bayes_pls_golite062009
transferase activity, transferring acyl groups	0.50269055590758	bayes_pls_golite062009
cystathionine beta-lyase activity	0.27933426619091	bayes_pls_golite062009
glycine hydroxymethyltransferase activity	0.271623203801431	bayes_pls_golite062009
O-phospho-L-serine:2-oxoglutarate aminotransferase activity	0.234399054481664	bayes_pls_golite062009
RNA binding	0.222777224430863	bayes_pls_golite062009
vitamin B6 binding	0.16957228630576	bayes_pls_golite062009
pyridoxal phosphate binding	0.16957228630576	bayes_pls_golite062009
methyltransferase activity	0.103986924428507	bayes_pls_golite062009
sulfurtransferase activity	0.0450490929307473	bayes_pls_golite062009
transferase activity, transferring one-carbon groups	0.0254276656964899	bayes_pls_golite062009

Predicted Domain #3

Region A:
Residues: [105-357]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 QATTPTDPRV LDTMLKFYTG LYGNPHSNTH SYGWETNTAV ENARAHVAKM INADPKEIIF  60
   61 TSGATESNNM VLKGVPRFYK KTKKHIITTR TEHKCVLEAA RAMMKEGFEV TFLNVDDQGL 120
  121 IDLKELEDAI RPDTCLVSVM AVNNEIGVIQ PIKEIGAICR KNKIYFHTDA AQAYGKIHID 180
  181 VNEMNIDLLS ISSHKIYGPK GIGAIYVRRR PRVRLEPLLS GGGQERGLRS GTLAPPLVAG 240
  241 FGEAARLMKK EFD

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	860.9897
Match:	1ecxA_
Description:	NifS-like protein/selenocysteine lyase
Matching Structure (courtesy of the PDB):

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

View Structure Prediction Details

Top Sequence Alignment Hits

Predicted functions: