Protein Overview: PET112

General Information:
Name(s) found:	PET112 / YBL080C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6319391\|ref\|NP_009473.1\| Protein required for mitochondrial translation; mutation is functionally complemented by a Bacillus subtilis ortholog; Pet112p [Saccharomyces cerevisiae] [NCBI NR] pir\|\|S45428 PET112 protein - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|585751\|sp\|P33893.2\|GATB_YEAST RecName: Full=Probable glutamyl-tRNA(Gln) amidotransferase subunit B, mitochondrial; Short=GLU-ADT subunit B; AltName: Full=Cytochrome oxidase assembly factor PET112; Flags: Precursor [NCBI NR] gi\|536129\|emb\|CAA84901.1\| PET112 [Saccharomyces cerevisiae] [NCBI NR] gi\|496701\|emb\|CAA56028.1\| pet112 protein [Saccharomyces cerevisiae] [NCBI NR] Glutamyl-tRNA(Gln) amidotransferase subunit B, mitochondrial OS=Saccharomyces cerevisiae GN=PET112 PE=1 SV=2 [Swiss-Prot] PET112 Protein required to maintain rho+ mitochondrial DNA [mips] Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; mutation is functionally complemented by the bacterial GatB ortholog [SGD] PET112, Chr II from 73064-74689, reverse complement [yeast_orfs2] Chr II from 73064-74689, reverse complement [yeastorf3.fasta] Chr II from 74689-73064, reverse complement [yeastorf5.fasta] (P33893) Probable glutamyl-tRNA(Gln) amidotransferase subunit B, mitochondrial precursor (EC 6.3.5.-) (GLU-ADT subunit B) (Cytochrome oxidase assembly factor PET112) [4932.FASTACCERV.txt] PET112 SGDID:S0000176, Chr II from 74692-73067, reverse complement, Verified ORF [yeastflysequest.fasta] Pet112p [Saccharomyces cerevisiae]gi\|496701\|emb\|CAA56028.1\| pet112 protein [Saccharomyces cerevisiae]gi\|536129\|emb\|CAA84901.1\| PET112 [Saccharomyces cerevisiae]gi\|585751\|sp\|P33893\|GATB_YEAST Probable glutamyl-tRNA(Gln) amidotransferase subunit B, mitoc [nrdb_11152004_Chlamydomonas] PET112 SGDID:S000000176, Chr II from 74692-73067, reverse complement, Verified ORF, "Protein required for mitochondrial translation; mutation is functionally complemented by a Bacillus subtilis ortholog" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] PET112 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Probable glutamyl-tRNA(Gln) amidotransferase subunit B, mitochondrial precursor (GLU-ADT subunit B) (Cytochrome oxidase assembly factor PET112) [nr-271106-contam.fasta] pet112 protein [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Protein required for mitochondrial translation; mutation is functionally complemented by a Bacillus subtilis ortholog; Pet112p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] PET112 SGDID:S000000176, Chr II from 74692-73067, reverse complement, Verified ORF, "Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; mutation is functionally complemented by the bacterial GatB ortholog" [SGD_S-cerevisiae_na_01-05-2010_reversed.fasta] [chemostatdb.fasta]
Organism:	Saccharomyces cerevisiae
Length:	541 amino acids
Gene Ontology:
Cellular Component:	mitochondrion [IDA ][IGI]
Biological Process:	aerobic respiration [IMP] mitochondrion organization [IMP] translation [IMP]
Molecular Function:	molecular_function [ND]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MLRLARFYSL ARTKAIHSHG APFRPEYALK CGLEIHTQLN TKNKLFSQST NSATSLVDAP  60
   61 NHHTSYYDIA LPGTQPVLNL EAILFAMKLS LALGSQVNSI SQFDRKHYFY GDQPQGYQLT 120
  121 QHYRPFARGG KINLSKELDD IDESAKEIGI LQLQIEQDTG KSHYTETDKD VITLVDLNRS 180
  181 NVPLIELVTK PDFSDIKQVR AFIKKYQNLV RHLHISSGDL ETGAMRVDVN LSINEYARVE 240
  241 LKNLPNTSSI INAIKYEYQR QVELISVGDT SSLMEPETRG WTGSSTVKLR SKETTIDYRY 300
  301 MPDPELPYIN LAPDVISGVR GLMPQLPDDI MRILMKKPYQ LSLKDAKILT YNSNQNDMYN 360
  361 HEALRSYYLD TFREFSKLAG ERSNAKLPTN WIIHEFLGDL NKLQIPLAKA KEILAPPVFA 420
  421 QFLKLLHEEV ISATSGKMLL FHILENFEQS NCQDLSIPDF SKLIEKFELH AINQVDPQEL 480
  481 MDLCNDVIAQ HTDDTFIRNL VTGKKKSSLK FLIGQGMRRS QGRIKANEFE KKFKEILNIQ 540
  541 W

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	(MIPS) Mewes HW, et al. (2004)		AEP1 AEP2 CBP6 CBS1 CBS2 MSS51 PET111 PET112 PET122 PET309 PET494 PET54 SUV3
View Details	Ho Y, et al. (2002)		CDC33 PET112 PRB1 SNF1 TFP1 YEL023C YGR016W YPK2
View Details	Qiu et al. (2008)		PET112 PET309

Mass Spectrometry Data

No mass spectrometry results found for this protein.

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	RAD51	PET112	1	Unpublished Fields Lab Data
View Screen	UBP6	PET112	1	Unpublished Fields Lab Data
View Screen	MIG1	PET112	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	PET112	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..271]	Pfam	135.229148	PET112 family, N terminal region No confident structure predictions are available.
2	View Details	[272..363]	ORFEUS	7.06	Annexin VI
3	View Details	[364..541]	ORFEUS	7.06	Annexin VI

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity	6.94703732461209	bayes_pls_golite062009
ligase activity	5.28014676041088	bayes_pls_golite062009
catalytic activity	1.08973967683802	bayes_pls_golite062009
binding	1.0468360119595	bayes_pls_golite062009
ammonia ligase activity	0.978763138251862	bayes_pls_golite062009
ligase activity, forming carbon-nitrogen bonds	0.789800177023595	bayes_pls_golite062009
acid-ammonia (or amide) ligase activity	0.632807059205458	bayes_pls_golite062009
nucleic acid binding	0.545649763280687	bayes_pls_golite062009
protein binding	0.440356087696859	bayes_pls_golite062009
kinase activity	0.21984983699175	bayes_pls_golite062009
transferase activity, transferring phosphorus-containing groups	0.132389278472779	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.95

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2