Abstract
A novel Bacillus gene was isolated and characterized. It encodes a homolog of Saccharomyces cerevisiae Pet112p, a protein that has no characterized relative and is dispensable for cell viability but required for mitochondrial translation. Expression of the Bacillus protein in yeast, modified to ensure mitochondrial targeting, partially complemented the phenotype of the pet112-1 mutation, demonstrating a high degree of evolutionary conservation for this as yet unidentified component of translation.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Bacillus subtilis / genetics*
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DNA, Fungal / genetics*
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DNA, Mitochondrial / genetics*
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Fungal Proteins / genetics
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Genes, Bacterial / genetics
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Mitochondrial Proteins
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Molecular Sequence Data
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Protein Biosynthesis / genetics*
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Recombinant Fusion Proteins
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Saccharomyces cerevisiae / genetics*
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Saccharomyces cerevisiae / growth & development
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Saccharomyces cerevisiae Proteins*
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Sequence Analysis, DNA
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Sequence Homology, Amino Acid
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Transaminases*
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Transcription Factors / genetics*
Substances
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DNA, Fungal
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DNA, Mitochondrial
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Fungal Proteins
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Mitochondrial Proteins
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Recombinant Fusion Proteins
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Saccharomyces cerevisiae Proteins
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Transcription Factors
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Transaminases
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PET112 protein, S cerevisiae
Associated data
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GENBANK/AE000058
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GENBANK/D90913
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GENBANK/L22072
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GENBANK/U00096
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GENBANK/U39689
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GENBANK/U49269
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GENBANK/U49790
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GENBANK/U62332
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GENBANK/U67473