Protein Overview: NFS1

General Information:
Name(s) found:	NFS1 / YCL017C [SGD]
Description(s) found: Found 26 descriptions. SHOW ALL	Cysteine desulfurase involved in iron-sulfur cluster (Fe/S) biogenesis; required for the post-transcriptional thio-modification of mitochondrial and cytoplasmic tRNAs; essential protein located predominantly in mitochondria [SGD]
Organism:	Saccharomyces cerevisiae
Length:	497 amino acids
Gene Ontology:
Cellular Component:	nucleus [IMP][ISS] mitochondrion [IDA ][TAS]
Biological Process:	cellular iron ion homeostasis [IMP] iron-sulfur cluster assembly [IDA][ISS][TAS]
Molecular Function:	tRNA sulfurtransferase activity [IMP] cysteine desulfurase activity [IDA] cystathionine gamma-lyase activity [ISS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MLKSTATRSI TRLSQVYNVP AATYRACLVS RRFYSPPAAG VKLDDNFSLE THTDIQAAAK  60
   61 AQASARASAS GTTPDAVVAS GSTAMSHAYQ ENTGFGTRPI YLDMQATTPT DPRVLDTMLK 120
  121 FYTGLYGNPH SNTHSYGWET NTAVENARAH VAKMINADPK EIIFTSGATE SNNMVLKGVP 180
  181 RFYKKTKKHI ITTRTEHKCV LEAARAMMKE GFEVTFLNVD DQGLIDLKEL EDAIRPDTCL 240
  241 VSVMAVNNEI GVIQPIKEIG AICRKNKIYF HTDAAQAYGK IHIDVNEMNI DLLSISSHKI 300
  301 YGPKGIGAIY VRRRPRVRLE PLLSGGGQER GLRSGTLAPP LVAGFGEAAR LMKKEFDNDQ 360
  361 AHIKRLSDKL VKGLLSAEHT TLNGSPDHRY PGCVNVSFAY VEGESLLMAL RDIALSSGSA 420
  421 CTSASLEPSY VLHALGKDDA LAHSSIRFGI GRFSTEEEVD YVVKAVSDRV KFLRELSPLW 480
  481 EMVQEGIDLN SIKWSGH

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Krogan NJ, et al. (2006)		ACP1 FMC1 ISD11 NFS1
View Details	Gavin AC, et al. (2006)		ISD11 NFS1 TDH3
View Details	Ho Y, et al. (2002)		ISU1 NFS1

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	ASF1	3906: TAP-tagged	Green EM, et al (2005)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	SRO77	NFS1	2	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	NFS1	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..70]	deduced	N/A	No confident structure predictions are available.
2	View Details	[71..104] [358..497]	PSI-BLAST	860.9897	NifS-like protein/selenocysteine lyase
3	View Details	[105..357]	PSI-BLAST	860.9897	NifS-like protein/selenocysteine lyase

Functions predicted (by domain):

Gene Ontology predictions

No functions predicted.

Term	Confidence	Notes
oxidoreductase activity, acting on the CH-NH2 group of donors, disulfide as acceptor	3.40354781002256	bayes_pls_golite062009
glycine dehydrogenase (decarboxylating) activity	3.40354781002256	bayes_pls_golite062009
transferase activity	2.59075614411581	bayes_pls_golite062009
L-aspartate:2-oxoglutarate aminotransferase activity	2.30133284041702	bayes_pls_golite062009
catalytic activity	2.29878871754893	bayes_pls_golite062009
transcription regulator activity	1.67701710033155	bayes_pls_golite062009
DNA binding	1.4774443071095	bayes_pls_golite062009
nucleic acid binding	1.39741122443159	bayes_pls_golite062009
cysteine desulfurase activity	1.30923778688541	bayes_pls_golite062009
transferase activity, transferring nitrogenous groups	1.28316822767388	bayes_pls_golite062009
transaminase activity	1.24990480711889	bayes_pls_golite062009
transcription factor activity	1.15775909410582	bayes_pls_golite062009
binding	1.09053123792737	bayes_pls_golite062009
L-alanine:2-oxoglutarate aminotransferase activity	1.06988190956423	bayes_pls_golite062009
alanine-oxo-acid transaminase activity	1.06988190956423	bayes_pls_golite062009
ligase activity	0.827063849687833	bayes_pls_golite062009
structural molecule activity	0.754675308623467	bayes_pls_golite062009
carbon-sulfur lyase activity	0.698312655247044	bayes_pls_golite062009
5-aminolevulinate synthase activity	0.54326874956263	bayes_pls_golite062009
cystathionine gamma-lyase activity	0.505859361763689	bayes_pls_golite062009
transferase activity, transferring acyl groups	0.50269055590758	bayes_pls_golite062009
cystathionine beta-lyase activity	0.27933426619091	bayes_pls_golite062009
glycine hydroxymethyltransferase activity	0.271623203801431	bayes_pls_golite062009
O-phospho-L-serine:2-oxoglutarate aminotransferase activity	0.234399054481664	bayes_pls_golite062009
RNA binding	0.222777224430863	bayes_pls_golite062009
vitamin B6 binding	0.16957228630576	bayes_pls_golite062009
pyridoxal phosphate binding	0.16957228630576	bayes_pls_golite062009
methyltransferase activity	0.103986924428507	bayes_pls_golite062009
sulfurtransferase activity	0.0450490929307473	bayes_pls_golite062009
transferase activity, transferring one-carbon groups	0.0254276656964899	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.97

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2