Protein Overview: ASN1

General Information:
Name(s) found:	ASN1 / YPR145W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|747902\|emb\|CAA88594.1\| asparagine synthetase [Saccharomyces cerevisiae] [NCBI NR] gi\|6325403\|ref\|NP_015471.1\| Asparagine synthetase, isozyme of Asn2p; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway; Asn1p [Saccharomyces cerevisiae] [NCBI NR] gi\|1351984\|sp\|P49089.2\|ASNS1_YEAST RecName: Full=Asparagine synthetase [glutamine-hydrolyzing] 1; AltName: Full=Glutamine-dependent asparagine synthetase 1 [NCBI NR] pir\|\|S52694 asparagine synthase (glutamine-hydrolysing) (EC 6.3.5.4) - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|1066479\|gb\|AAB68284.1\| Asn1p: Asparagine synthetase [Saccharomyces cerevisiae] [NCBI NR] Asparagine synthetase [glutamine-hydrolyzing] 1 OS=Saccharomyces cerevisiae GN=ASN1 PE=1 SV=2 [Swiss-Prot] ASN1 Asparagine synthetase [mips] Asparagine synthetase, isozyme of Asn2p; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway [SGD] Chr XVI from 822614-824332 [hmr_yeast5_DM3-1_pombe.fasta] Chr XVI from 822615-824333 [yeastorf3.fasta] ASN1, Chr XVI from 822615-824333 [yeast_orfs2] ASN1 SGDID:S0006349, Chr XVI from 822614-824332, Verified ORF [yeastflysequest.fasta] ASN1 SGDID:S0006349, Chr XVI from 822616-824334, Verified ORF [10112005cervSGD.fasta] Asn1p [Saccharomyces cerevisiae]gi\|1066479\|gb\|AAB68284.1\| Asn1p: Asparagine synthetase [Saccharomyces cerevisiae]gi\|747902\|emb\|CAA88594.1\| asparagine synthetase [Saccharomyces cerevisiae]gi\|1351984\|sp\|P49089\|ASN1_YEAST Asparagine synthetase [glutamine- [nrdb_11152004_Chlamydomonas] ASN1 SGDID:S000006349, Chr XVI from 822616-824334, Verified ORF, "Asparagine synthetase, isozyme of Asn2p; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] Asparagine synthetase, isozyme of Asn2p; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway; Asn1p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Asn1p: Asparagine synthetase [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Asparagine synthetase [glutamine-hydrolyzing] 1 (Glutamine-dependent asparagine synthetase 1) [nr-271106-contam.fasta] asparagine synthetase [Saccharomyces cerevisiae] [nr-271106-contam.fasta] [chemostatdb.fasta] Asparagine synthetase [glutamine-hydrolyzing] 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=ASN1 PE=1 SV=2 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] ASN1 SGDID:S000006349, Chr XVI from 822620-824338, Genome Release 64-1-1, Verified ORF, "Asparagine synthetase, isozyme of Asn2p; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway" [yeast-030211-contam.fasta] ASN1 SGDID:S000006349, Chr XVI from 822620-824338, Genome Release 64-2-1, Verified ORF, "Asparagine synthetase; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway; ASN1 has a paralog, ASN2, that arose from the whole genome duplication" [20141208_orf_trans.fasta] TR5410\|c0_g1_i2\|g.3742 ORF TR5410\|c0_g1_i2\|g.3742 TR5410\|c0_g1_i2\|m.3742 type:complete len:573 (+) TR5410\|c0_g1_i2:203-1921(+) [DBVPG6044_S_cerevisiae-Sep2015-contam.fasta] YPR145W SGDID:S000006349, Chr XVI from 822620-824338, Genome Release 64-1-1, Verified ORF, "Asparagine synthetase, isozyme of Asn2p; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta] asmbl_3717\|g.2351 ORF asmbl_3717\|g.2351 asmbl_3717\|m.2351 type:complete len:573 (+) asmbl_3717:202-1920(+) [DBVPG6044_Trinity_v2_2_0_PASA_6May2016-contam.fasta] ID=asmbl_3317\|g.2272 ORF ID=asmbl_3317\|g.2272 ID=asmbl_3317\|m.2272 type:complete len:573 (+) ID=asmbl_3317:202-1920(+) [DBVPG6044_quiverGenomes_Trinity_v2_2_0_PASA_2June2016-contam.fasta] ID=asmbl_3108\|g.1898 ORF ID=asmbl_3108\|g.1898 ID=asmbl_3108\|m.1898 type:complete len:573 (+) ID=asmbl_3108:199-1917(+) [Y12_quiver_Trinity_v2_2_0_PASA_2June2016-contam.fasta]
Organism:	Saccharomyces cerevisiae
Length:	572 amino acids
Gene Ontology:
Cellular Component:	cytoplasm [IDA]
Biological Process:	asparagine biosynthetic process [TAS]
Molecular Function:	asparagine synthase (glutamine-hydrolyzing) activity [IMP][ISS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MCGIFAAFRH EDVHRYKPKA LQLSKRIRHR GPDWSGNAIK NSTIFVHERL AIVGVESGAQ  60
   61 PITSSDGEYM LCVNGEIYNH IQLREECADY EFGTLSDCEP IIPMYLKHDI DAPKYLDGMF 120
  121 AWTLYDAKQD RIVAARDPIG ITTLYMGRSS ASPKTVYFAS ELKCLTDDCD TITAFPPGHV 180
  181 YDSKTDKITR YFTPDWLDEK RIPSTPIDYM AIRHSLEKAV RKRLMAEVPY GVLLSGGLDS 240
  241 SLIASIAARE TAKATNDVEP STYDSKARHL AGIDDDGKLH TAGWTSLHSF AIGLPNAPDL 300
  301 QAARKVAKFI GSIHHEHTFT LQEGLDALDD VIYHLETYDV TTIRASTPMF LLSRKIKAQG 360
  361 VKMVLSGEGS DEIFGGYLYF AQAPSAAEFH TESVQRVKNL HLADCLRANK STMAWGLEAR 420
  421 VPFLDREFLQ LCMNIDPNEK MIKPKEGRIE KYILRKAFDT TGEPDAKPYL PEEILWRQKE 480
  481 QFSDGVGYSW IDGLKDTAEA VISDEMFASP KAEWGSDIPT TKEAFWYRLK FDALFPQKTV 540
  541 ADTVMRWIPK ADWGCAEDPS GRYAQIHEKH IE

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Qiu et al. (2008)		ASN1 ASN2

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)
View Run	MLP2	#25 Asynchronous Prep3-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#32 Asynchronous Prep-No Phosphopeptide enrichment	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	YRB2	ASN1	1	Unpublished Fields Lab Data
View Screen	SPT21	ASN1	1	Unpublished Fields Lab Data

Microscopy / Localization Data

New Feature: Upload Your Own Microscopy Data

	PROTEIN(S)	PUBLICATION
View Data	ASN1	Huh WK, et al. (2003)

Protein Structure Data

[What does the above image mean?]
[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..192]	PSI-BLAST	1614.0	Asparagine synthetase B, C-terminal domain; Asparagine synthetase B, N-terminal domain
2	View Details	[193..572]	PSI-BLAST	1614.0	Asparagine synthetase B, C-terminal domain; Asparagine synthetase B, N-terminal domain

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
asparagine synthase (glutamine-hydrolyzing) activity	3.86770527955716	bayes_pls_golite062009
asparaginase activity	2.94567124791739	bayes_pls_golite062009
peptidase activity	1.76298553502805	bayes_pls_golite062009
DNA binding	1.66643360311025	bayes_pls_golite062009
nucleic acid binding	1.65897778831304	bayes_pls_golite062009
endopeptidase activity	1.58381740825719	bayes_pls_golite062009
peptidase activity, acting on L-amino acid peptides	1.56290563467621	bayes_pls_golite062009
acid-ammonia (or amide) ligase activity	1.41109692769794	bayes_pls_golite062009
N4-(beta-N-acetylglucosaminyl)-L-asparaginase activity	1.2528157874993	bayes_pls_golite062009
catalytic activity	1.19663085909195	bayes_pls_golite062009
binding	1.18139971903068	bayes_pls_golite062009
carbon-nitrogen ligase activity, with glutamine as amido-N-donor	1.17174228126736	bayes_pls_golite062009
hydrolase activity	1.05106912722792	bayes_pls_golite062009
protein binding	1.03373728327879	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.98

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2