Protein Overview: RIM20

General Information:
Name(s) found:	RIM20 / YOR275C [SGD]
Description(s) found: Found 30 descriptions. SHOW ALL	Protein involved in proteolytic activation of Rim101p in response to alkaline pH; PalA/AIP1/Alix family member; interaction with the ESCRT-III subunit Snf7p suggests a relationship between pH response and multivesicular body formation [SGD]
Organism:	Saccharomyces cerevisiae
Length:	661 amino acids
Gene Ontology:
Cellular Component:	nucleus [IDA] cytoplasm [IDA]
Biological Process:	proteolysis [IMP] protein processing [IDA][IGI][IMP] invasive growth in response to glucose limitation [IMP] sporulation resulting in formation of a cellular spore [IMP]
Molecular Function:	molecular_function [ND]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSELLAIPLK RTLEVDFATE LSKLIDTTSF QTASFFQSDI LKVVDARNNA IAPDISIDGL  60
   61 SALKEYYVIL LQLEKKFPNN QIEFTWFQTL SQKSRGTSQY SLQWEKLTII YNIGCMYSLL 120
  121 ALNSNNDAAE SLKTSCLYFQ NAAGCFKHVL DHQKNLETIP VVDDATLNAL TSLMLAQAQE 180
  181 CFWFKAVQDK HKDSLIAKLS QQIVDFYCEA INDAQRGKLI RSDWINHLKA KKAYFSAVTY 240
  241 YRIALSFNEK KQFGNVVKAL QMGLQFINES TLSSQAKFKT VVESSLKEAQ RDNEFIYLQE 300
  301 VPSELPSIKP ALMVKPSSSA TLLPSIKKDE TLFKDLIPIE VMEYCTAYNE RQDEYVEQRV 360
  361 TNPLASLNKL LKESLTTFQI PQGLTKVSEA ELSHYQASLN NLLINNKNVQ VQLDNIEQIL 420
  421 NEEAFTDNQL RLKHGTLNWT LPESSTTNTA YYEKLKKLRG YLDEGSAIDK QTNELFQSID 480
  481 KNLIGSEIRL PESNDPLTNK IKMIIQERND YIDRTRRKSS EYRILPKIIT SYKKNGTVDF 540
  541 EPIFIGHLKY FDEDLRYVNS TKEENIKLIE EVNLSKKNNP GRSGIEPKKM VRIDPRELYI 600
  601 EDLRYSFKLL DEVKENLSAG TAFYENLITS TSNLYNEVQE YDTARRAEKA RLDKSLTFED 660
  661 Q

Protein Complex Data

No complex found for this protein.

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)
View Run	RLF2	identification data - first search in cascade	Green EM, et al (2005)
View Run	MLP2	#03 Alpha Factor Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	CNS1	RIM20	1	Unpublished Fields Lab Data
View Screen	RAD51	RIM20	1	Unpublished Fields Lab Data
View Screen	SNF4	RIM20	1	Unpublished Fields Lab Data
View Screen	LSM8	RIM20	1	Unpublished Fields Lab Data
View Screen	STE3	RIM20	1	Unpublished Fields Lab Data
View Screen	HMG1	RIM20	1	Unpublished Fields Lab Data
View Screen	SPO1	RIM20	1	Unpublished Fields Lab Data
View Screen	SAS3	RIM20	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	RIM20	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..148]	Pfam	64.853872	BRO1-like domain No confident structure predictions are available.
2	View Details	[149..468]	PSI-BLAST	25.221849	Heavy meromyosin subfragment
3	View Details	[469..661]	PSI-BLAST	9.154902	Tropomyosin

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.98

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2