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Protein Overview: BNI4

General Information:
Name(s) found: BNI4 / YNL233W [SGD]
Description(s) found:
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  • gi|6324096|ref|NP_014166.1| Targeting subunit for Glc7p protein phosphatase, localized to the bud neck, required for localization of chitin synthase III to the bud neck via interaction with the chitin synthase III regulatory subunit Skt5p; Bni4p [Saccharo [NCBI NR]
  • pir||S63199 BNI4 protein - yeast (Saccharomyces cerevisiae) [NCBI NR]
  • gi|1730833|sp|P53858.1|BNI4_YEAST RecName: Full=Protein BNI4 [NCBI NR]
  • gi|1302261|emb|CAA96138.1| BNI4 [Saccharomyces cerevisiae] [NCBI NR]
  • gi|1183981|emb|CAA93365.1| N1146 [Saccharomyces cerevisiae] [NCBI NR]
  • Protein BNI4 OS=Saccharomyces cerevisiae GN=BNI4 PE=1 SV=1 [Swiss-Prot]
  • BNI4 Bud neck involved [mips]
  • Targeting subunit for Glc7p protein phosphatase, localized to the bud neck, required for localization of chitin synthase III to the bud neck via interaction with the chitin synthase III regulatory subunit Skt5p [SGD]
  • Chr XIV from 211921-214599 [yeastorf3.fasta]
  • (P53858) BNI4 protein [4932.FASTACCERV.txt]
  • BNI4, Chr XIV from 211921-214599 [yeast_orfs2]
  • BNI4 SGDID:S0005177, Chr XIV from 211921-214599, Verified ORF [yeastflysequest.fasta]
  • Bni4p [Saccharomyces cerevisiae]gi|1183981|emb|CAA93365.1| N1146 [Saccharomyces cerevisiae]gi|1302261|emb|CAA96138.1| BNI4 [Saccharomyces cerevisiae]gi|2131937|pir||S63199 BNI4 protein - yeast (Saccharomyces cerevisiae)gi|1730833|sp|P53858|BNI4_YEAST [nrdb_11152004_Chlamydomonas]
  • BNI4 SGDID:S000005177, Chr XIV from 211923-214601, Verified ORF, "Targeting subunit for Glc7p protein phosphatase, localized to the bud neck, required for localization of chitin synthase III to the bud neck via interaction with the chitin synthase III reg [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta]
  • BNI4 [Saccharomyces cerevisiae] [nr-271106-contam.fasta]
  • Targeting subunit for Glc7p protein phosphatase, localized to the bud neck, required for localization of chitin synthase III to the bud neck via interaction with the chitin synthase III regulatory subunit Skt5p; Bni4p [Saccharomyces cerevisiae] [nr-271106-contam.fasta]
  • Protein BNI4 [nr-271106-contam.fasta]
  • N1146 [Saccharomyces cerevisiae] [nr-271106-contam.fasta]
  • BNI4 SGDID:S000005177, Chr XIV from 211923-214601, Verified ORF, "Targeting subunit for Glc7p protein phosphatase, localized to the bud neck, required for localization of chitin synthase III to the bud neck via interaction with the chitin synthase III regulatory subunit Skt5p" [yeast-bovrabprots.fasta]
  • [chemostatdb.fasta]
  • Protein BNI4 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=BNI4 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta]
  • BNI4 SGDID:S000005177, Chr XIV from 211922-214600, Genome Release 64-1-1, Verified ORF, "Targeting subunit for Glc7p protein phosphatase, localized to the bud neck, required for localization of chitin synthase III to the bud neck via interaction with the chitin synthase III regulatory subunit Skt5p" [yeast-030211-contam.fasta]
  • BNI4 SGDID:S000005177, Chr XIV from 211922-214600, Genome Release 64-2-1, Verified ORF, "Targeting subunit for Glc7p protein phosphatase; localized to the bud neck, required for localization of chitin synthase III to the bud neck via interaction with the chitin synthase III regulatory subunit Skt5p" [20141208_orf_trans.fasta]
  • YNL233W SGDID:S000005177, Chr XIV from 211922-214600, Genome Release 64-1-1, Verified ORF, "Targeting subunit for Glc7p protein phosphatase, localized to the bud neck, required for localization of chitin synthase III to the bud neck via interaction with the chitin synthase III regulatory subunit Skt5p" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
    • Organism: Saccharomyces cerevisiae
    Length: 892 amino acids

    Gene Ontology:
    Cellular Component: incipient cellular bud site [IDALink to PubMed article]
    cellular bud neck [IDALink to PubMed article]
    septin ring [IDALink to PubMed article][IPILink to PubMed article]
    Biological Process: barrier septum formation [IGILink to PubMed article]
    chitin biosynthetic process [IGILink to PubMed article][IMPLink to PubMed article][TASLink to PubMed article]
    asymmetric protein localization [IDALink to PubMed article][IMPLink to PubMed article]
    Molecular Function: protein binding [IDALink to PubMed article]


    Sequence:
    Sequence:
    [PDR BLAST]
    [ProtParam] 
          1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSDSISDSKS SELLNSTFYS STSINTLDHA RTFRNSLILK EISDQSLNSS IKPCESVLDR  60
   61 DVESSVLQRS FGESNARDSE VQTVNMTTSP SLSALADILN ERSKYADQKT RKAQNIESSI 120
  121 IEEEEEAEEQ NNSINYHEDI TGSRLSVREE ANENLAMTSP NLIDIDGSNS IQVAPLSLPS 180
  181 FEEPDFLSTP RVKPDSQGPR SKVSTRRTIL ERDNNLPVKR EENTIINSET ESTTHSAPFL 240
  241 KEDPKPSPPS SKLYNPKVRL NKAEARKYTD SSAQRTTSAG SVLEDTSMHK KKKSIFSFLK 300
  301 KKEPKPVIGN NSVTNEKNKM SSSSTFSMNI QTSLKTPEKL KKKSHSSSSI FNSFLKGKIE 360
  361 TSDSPRKEPM RQKKRTPKSK DKKQDTEQII DAASVLSTES PLLRKNHDDT PVKIDHVTRS 420
  421 IDQRKPTPLN MDLILGGDKQ INTPLQEHVR EDDDAKNDLQ LPTKDNFLSL DYEAPSPAFS 480
  481 KHDTGEVLFP KFLDNHEVDS IVSLERTRST KSNKRSSMNS QRRSLTDTLS IKAQSEGMFI 540
  541 TEASSVVLST PDLTKSPASS ILKNGRFEYS DNFSREHSYE GTTNEDFLDI KDDSGPLKKD 600
  601 DIFLESIEQK FDQLVMASDE EKTEVERDVP KPREEPLKKD SERQSVFADD DNELISDIME 660
  661 FASFINFGDD DLNLDLDLGD TTASYATETP EPVGNDEVNR SGTFDTRNNK EDSYKERETQ 720
  721 SYSAAGATTY GDERQGQLHT FEQDGSEIND NEFENEDFNK HIEQPIEVTP RNNAYLPEFE 780
  781 PNRPVSMSFK GLKAPRMNTS FIDSMTPDSP VKSDLTSLGE VYVNSNNDQG VRFSSQIILY 840
  841 DTYGEFEYDR HPEISTCNQL TPQLAQMIKL ELNELKSAME VHDDSRCYTH FY

    Protein Complex Data

      PUBLICATION TOPOLOGY COCOMPLEXED PROTEINS
    View Details Krogan NJ, et al. (2006) BNI4 BUD14 GIP3 GLC7 GLC8 HER1 PPZ1 PUF3 SDS22 SOL1 SOL2 YLR257W YPI1
    View Details Qiu et al. (2008) BNI4 BNI5 CDC11 CDC3 MYO2 SKT5 SLA1 SLA2

    Mass Spectrometry Data

    The following runs contain data for this protein:

      BAIT COMMENTS PUBLICATION
    View Run MLP2 #31 Asynchronous Prep (Protease cleavage) Keck JM, et al. (2011)
    View Run MLP2 #12 Mitotic Prep1-TiO2 Flowthrough Keck JM, et al. (2011)
    View Run MLP2 #02 Alpha Factor Prep1-TiO2 enriched, new search criteria Keck JM, et al. (2011)
    View Run MLP2 #11 Mitotic Prep1-TiO2 enriched, new search criteria Keck JM, et al. (2011)

    Yeast Two-Hybrid Data

    The following interactions contain this protein:

    SHOWING SINGLE HITS. [ Hide Single Hits ]

    [View our yeast two-hybrid interpretation guidelines.]

      BAIT PREY HITS PUBLICATION
    View Screen GLC7 BNI4 2 Uetz P, et al. (2000)
    View Screen MSB2 BNI4 2 Drees BL, et al. (2001)

    Microscopy / Localization Data

    New Feature: Upload Your Own Microscopy Data

      PROTEIN(S) PUBLICATION
    View Data BNI4 Sundin BA, et al. (2004)
    View Data BNI4 Sundin BA, et al. (2004)
    View Data BNI4 Huh WK, et al. (2003)

    Protein Structure Data


    [What does the above image mean?]
    [View Top Sequence Alignments] [Show Ginzu Version Information]

    Domains predicted:

    #   Region(s) Method Confidence Match Description
    1 View Details [1..892] deduced N/A No confident structure predictions are available.


    Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076


    Philius Transmembrane Prediction:

    Protein predicted to be: GLOBULAR (No transmembrane regions or signal peptide)
    Confidence of classification: 0.99

    Source: Reynolds et al. (2008)

    YRC Informatics Platform - Version 3.0
    Created and Maintained by: Michael Riffle