Protein Overview: NIS1

General Information:
Name(s) found:	NIS1 / YNL078W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|791106\|emb\|CAA60180.1\| unknown [Saccharomyces cerevisiae] [NCBI NR] gi\|6324251\|ref\|NP_014321.1\| Protein localized in the bud neck at G2/M phase; physically interacts with septins; possibly involved in a mitotic signaling network; Nis1p [Saccharomyces cerevisiae] [NCBI NR] gi\|1730746\|sp\|P53939.1\|NIS1_YEAST RecName: Full=Protein NIS1; AltName: Full=Neck protein interacting with septins protein 1; AltName: Full=Jumonji domain-interacting protein 1 [NCBI NR] gi\|1301969\|emb\|CAA95952.1\| unnamed protein product [Saccharomyces cerevisiae] [NCBI NR] pir\|\|S53900 hypothetical protein YNL078w - yeast (Saccharomyces cerevisiae) [NCBI NR] Uncharacterized protein YNL078W - Saccharomyces cerevisiae (Baker's yeast) [Swiss-Prot] Protein NIS1 OS=Saccharomyces cerevisiae GN=NIS1 PE=1 SV=1 [Swiss-Prot] NIS1 Non-essential role in the mitotic signaling network [mips] Protein localized in the bud neck at G2/M phase; physically interacts with septins; possibly involved in a mitotic signaling network [SGD] YNL078W, Chr XIV from 479765-480988 [yeast_orfs2] Chr XIV from 479765-480988 [yeastorf3.fasta] (P53939) Hypothetical 45.9 kDa protein in TPM1-MKS1 intergenic region [4932.FASTACCERV.txt] NIS1 SGDID:S0005022, Chr XIV from 479767-480990, Verified ORF [yeastflysequest.fasta] Nis1p [Saccharomyces cerevisiae]gi\|791106\|emb\|CAA60180.1\| unknown [Saccharomyces cerevisiae]gi\|1301969\|emb\|CAA95952.1\| unnamed protein product [Saccharomyces cerevisiae]gi\|1077199\|pir\|\|S53900 hypothetical protein YNL078w - yeast (Saccharomyces cerevisi [nrdb_11152004_Chlamydomonas] NIS1 SGDID:S000005022, Chr XIV from 479769-480992, Verified ORF, "Protein localized in the bud neck at G2/M phase; physically interacts with septins; possibly involved in a mitotic signaling network" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] unnamed protein product [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Protein localized in the bud neck at G2/M phase; physically interacts with septins; possibly involved in a mitotic signaling network; Nis1p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Uncharacterized protein YNL078W [nr-271106-contam.fasta] unknown [Saccharomyces cerevisiae] [nr-271106-contam.fasta] NIS1 SGDID:S000005022, Chr XIV from 479770-480993, Verified ORF, "Protein localized in the bud neck at G2/M phase; physically interacts with septins; possibly involved in a mitotic signaling network" [SGD_S-cerevisiae_na_01-05-2010_reversed.fasta] [chemostatdb.fasta] Protein NIS1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=NIS1 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] NIS1 SGDID:S000005022, Chr XIV from 479768-480991, Genome Release 64-1-1, Verified ORF, "Protein localized in the bud neck at G2/M phase; physically interacts with septins; possibly involved in a mitotic signaling network" [yeast-030211-contam.fasta] NIS1 SGDID:S000005022, Chr XIV from 479768-480991, Genome Release 64-2-1, Verified ORF, "Protein localized in the bud neck at G2/M phase; physically interacts with septins; possibly involved in a mitotic signaling network" [20141208_orf_trans.fasta] YNL078W SGDID:S000005022, Chr XIV from 479768-480991, Genome Release 64-1-1, Verified ORF, "Protein localized in the bud neck at G2/M phase; physically interacts with septins; possibly involved in a mitotic signaling network" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	407 amino acids
Gene Ontology:
Cellular Component:	nucleus [IDA] cellular bud neck [IDA]
Biological Process:	regulation of mitosis [IPI]
Molecular Function:	molecular_function [ND]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 METYETSIGT QSYPPTLFPP PLGTGGFTTS GYIHALVDST SNSNSNSNTN SNTNSNTNSN  60
   61 SDTKIPIVQI SDDSHITHDS FKPYMEYHDA SHLRNRNISK ADQVESTEVM EQFTQWSNYK 120
  121 MRSRSPTINA KPIRHTSQRR TDFTSKNELS KFSKNHNFIF HKGFLKRQHS IRREDRQAKV 180
  181 RSRFRSKKEL TSVLNYIELE QMDIANVLAS QPVNLHAIRN LTSRDPAVTP IPFLRSQMYA 240
  241 TSSRPPYLRN RSISRKLPKS QPGSLPTTMP ATATKTIKQN STTPTTRSVY NKNVGRSNTS 300
  301 PSVLYHPKRR GKLNTKSHAR KEQLLLELWR EYLMLVITQR TQLRLTLLCS PGSASNESSV 360
  361 CSSNASDLDM SLLSTPSSLF QMAGETKSNP IIIPDSQDDS ILSSDPF

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		NAP1 NBA1 NIS1
View Details	Krogan NJ, et al. (2006)		NBA1 NIS1
View Details	Gavin AC, et al. (2006)		CYS4 NAP1 NBA1 NIS1 SSC1
View Details	Gavin AC, et al. (2002)		GIN4 HTZ1 KAP114 KCC4 LYS12 NAP1 NBA1 NIS1 SSC1 YRA1
View Details	Ho Y, et al. (2002)		ELP2 ELP3 IKI3 NIS1 RSF2

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	ZDS1	Sample 2- cpn2 from june 2005	McCusker D, et al (2007)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	NSE5	NIS1	1	Hazbun TR, et al. (2003)
View Screen	RAD1	NIS1	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	NIS1	Huh WK, et al. (2003)

Protein Structure Data

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Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..407]	deduced	N/A	No confident structure predictions are available.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.90

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2