Protein Overview: GCV2

General Information:
Name(s) found:	GCV2 / YMR189W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|676871\|gb\|AAB18933.1\| glycine decarboxylase [NCBI NR] gi\|642281\|emb\|CAA87810.1\| putative glycine dehydrogenase [Saccharomyces cerevisiae] [NCBI NR] gi\|6323843\|ref\|NP_013914.1\| P subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; expression is regulated by levels of 5,10-methylene-THF in the cytoplasm; Gcv2p [Saccharomyces cerevisi [NCBI NR] gi\|1589242\|prf\|\|2210375A Gly decarboxylase:SUBUNIT=P [NCBI NR] gi\|1346120\|sp\|P49095.1\|GCSP_YEAST RecName: Full=Glycine dehydrogenase [decarboxylating], mitochondrial; AltName: Full=Glycine decarboxylase; AltName: Full=Glycine decarboxylase complex subunit P; AltName: Full=Glycine cleavage system P-protein; Flags: Pre [NCBI NR] pir\|\|S50917 aminomethyltransferase (EC 2.1.2.10) - yeast (Saccharomyces cerevisiae) [NCBI NR] Glycine dehydrogenase [decarboxylating], mitochondrial OS=Saccharomyces cerevisiae GN=GCV2 PE=1 SV=1 [Swiss-Prot] GCV2 Glycine decarboxylase P subunit [mips] P subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; expression is regulated by levels of 5,10-methylene-THF in the cytoplasm [SGD] GCV2, Chr XIII from 637499-640603 [yeast_orfs2] Chr XIII from 637499-640603 [yeastorf3.fasta] (P49095) Glycine dehydrogenase [decarboxylating], mitochondrial precursor (EC 1.4.4.2) (Glycine decarboxylase) (Glycine cleavage system P-protein) [4932.FASTACCERV.txt] GCV2 SGDID:S0004801, Chr XIII from 637499-640603, Verified ORF [yeastflysequest.fasta] Gcv2p [Saccharomyces cerevisiae]gi\|642281\|emb\|CAA87810.1\| putative glycine dehydrogenase [Saccharomyces cerevisiae]gi\|1346120\|sp\|P49095\|GCSP_YEAST Glycine dehydrogenase [decarboxylating], mitochondrial precursor (Glycine decarboxylase) (Glycine cleavage [nrdb_11152004_Chlamydomonas] GCV2 SGDID:S000004801, Chr XIII from 637499-640603, Verified ORF, "P subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; expression is regulated by levels of levels of 5,10-methylene-TH [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] GCV2 SGDID:S000004801, Chr XIII from 637499-640603, Verified ORF, "P subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; expression is regulated by levels of 5,10-methylene-THF in the c [SGD_S-cerevisiae_na_08-19-2008_con_reversed.fasta] Gly decarboxylase:SUBUNIT=P [nr-271106-contam.fasta] glycine decarboxylase [nr-271106-contam.fasta] P subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; expression is regulated by levels of levels of 5,10-methylene-THF in the cytoplasm; Gcv2p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Glycine dehydrogenase [decarboxylating], mitochondrial precursor (Glycine decarboxylase) (Glycine cleavage system P-protein) (Glycine decarboxylase complex subunit P) [nr-271106-contam.fasta] putative glycine dehydrogenase [Saccharomyces cerevisiae] [nr-271106-contam.fasta] GCV2 SGDID:S000004801, Chr XIII from 637499-640603, Verified ORF, "P subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; expression is regulated by levels of 5,10-methylene-THF in the cytoplasm" [yeast-bovrabprots.fasta] GCV2 SGDID:S000004801, Chr XIII from 637499-640603, Verified ORF, "P subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; expression is regulated by levels of levels of 5,10-methylene-THF in the cytoplasm" [yeast-contam_custom.fasta] [chemostatdb.fasta] Glycine dehydrogenase [decarboxylating], mitochondrial OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=GCV2 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] GCV2 SGDID:S000004801, Chr XIII from 637500-640604, Genome Release 64-1-1, Verified ORF, "P subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; expression is regulated by levels of 5,10-methylene-THF in the cytoplasm" [yeast-030211-contam.fasta] GCV2 SGDID:S000004801, Chr XIII from 637500-640604, Genome Release 64-2-1, Verified ORF, "P subunit of the mitochondrial glycine decarboxylase complex; glycine decarboxylase is required for the catabolism of glycine to 5,10-methylene-THF; expression is regulated by levels of 5,10-methylene-THF in the cytoplasm" [20141208_orf_trans.fasta] TR566\|c0_g1_i1\|g.341 ORF TR566\|c0_g1_i1\|g.341 TR566\|c0_g1_i1\|m.341 type:complete len:1035 (+) TR566\|c0_g1_i1:153-3257(+) [Y12_S_cerevisiae-Sep2015-contam.fasta] YMR189W SGDID:S000004801, Chr XIII from 637500-640604, Genome Release 64-1-1, Verified ORF, "P subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; expression is regulated by levels of 5,10-methylene-THF in the cytoplasm" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta] Glycine dehydrogenase (decarboxylating), mitochondrial OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=GCV2 PE=1 SV=1 [uniprot_sprot.fasta.20160308] ID=asmbl_4270\|g.2422 ORF ID=asmbl_4270\|g.2422 ID=asmbl_4270\|m.2422 type:complete len:1035 (+) ID=asmbl_4270:152-3256(+) [Y12_quiver_Trinity_v2_2_0_PASA_2June2016-contam.fasta]
Organism:	Saccharomyces cerevisiae
Length:	1034 amino acids
Gene Ontology:
Cellular Component:	mitochondrion [IDA ][TAS] glycine cleavage complex [TAS]
Biological Process:	glycine catabolic process [IC] one-carbon metabolic process [IGI][IMP]
Molecular Function:	glycine dehydrogenase (decarboxylating) activity [IEP][IMP]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MLRTRVTALL CRATVRSSTN YVSLARTRSF HSQSILLKTA ATDITSTQYS RIFNPDLKNI  60
   61 DRPLDTFARR HLGPSPSDVK KMLKTMGYSD LNAFIEELVP PNILKRRPLK LEAPSKGFCE 120
  121 QEMLQHLEKI ANKNHYKVKN FIGKGYYGTI LPPVIQRNLL ESPEWYTSYT PYQPEISQGR 180
  181 LEALLNFQTV VSDLTGLPVA NASLLDEGTA AGEAMLLSFN ISRKKKLKYV IDKKLHQQTK 240
  241 SVLHTRAKPF NIEIIEVDCS DIKKAVDVLK NPDVSGCLVQ YPATDGSILP PDSMKQLSDA 300
  301 LHSHKSLLSV ASDLMALTLL KPPAHYGADI VLGSSQRFGV PMGYGGPHAA FFAVIDKLNR 360
  361 KIPGRIVGIS KDRLGKTALR LALQTREQHI KRDKATSNIC TAQALLANVA SSYCVYHGPK 420
  421 GLQNISRRIF SLTSILANAI ENDSCPHELI NKTWFDTLTI KLGNGISSEQ LLDKALKEFN 480
  481 INLFAVDTTT ISLALDETTT KADVENLLKV FDIENSSQFL SEDYSNSFPR EFQRTDEILR 540
  541 NEVFHMHHSE TAMLRYLHRL QSRDLSLANS MIPLGSCTMK LNSTVEMMPI TWPQFSNIHP 600
  601 FQPSNQVQGY KELITSLEKD LCSITGFDGI SLQPNSGAQG EYTGLRVIRS YLESKGENHR 660
  661 NVCLIPVSAH GTNPASAAMA GLKVVPVNCL QDGSLDLVDL KNKAEQHSKE LAAVMITYPS 720
  721 TYGLFEPGIQ HAIDIVHSFG GQVYLDGANM NAQVGLTSPG DLGADVCHLN LHKTFSIPHG 780
  781 GGGPAGAPIC VKSHLIPHLP KHDVVDMITG IGGSKSIDSV SSAPYGNALV LPISYAYIKM 840
  841 MGNEGLPFSS VIAMLNSNYM MTRLKDHYKI LFVNEMSTLK HCAHEFIVDL REYKAKGVEA 900
  901 IDVAKRLQDY GFHAPTLAFP VPGTLMIEPT ESENLEELDR FCDAMISIKE EINALVAGQP 960
  961 KGQILKNAPH SLEDLITSSN WDTRGYTREE AAYPLPFLRY NKFWPTVARL DDTYGDMNLI1020
 1021 CTCPSVEEIA NETE

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	(MIPS) Mewes HW, et al. (2004)		GCV1 GCV2 GCV3 LPD1
View Details	Qiu et al. (2008)		GCV2 LPD1

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)
View Run	MLP2	#26 Asynchronous Prep4-TiO2 Phosphopeptide enriched, Steps1-2	Keck JM, et al. (2011)
View Run	MLP2	#03 Alpha Factor Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	RPN9	GCV2	1	Unpublished Fields Lab Data
View Screen	IST3	GCV2	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	GCV2	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..111]	deduced	N/A	No confident structure predictions are available.
2	View Details	[112..146] [426..539]	PSI-BLAST	65.522879	NifS-like protein/selenocysteine lyase
3	View Details	[147..425]	PSI-BLAST	65.522879	NifS-like protein/selenocysteine lyase
4	View Details	[540..850] [882..894]	PSI-BLAST	63.0	Cystathionine gamma-synthase, CGS
5	View Details	[851..881] [895..1034]	PSI-BLAST	63.0	Cystathionine gamma-synthase, CGS

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
glycine dehydrogenase (decarboxylating) activity	8.00572280567771	bayes_pls_golite062009
oxidoreductase activity, acting on the CH-NH2 group of donors, disulfide as acceptor	8.00572280567771	bayes_pls_golite062009
oxidoreductase activity, acting on the CH-NH2 group of donors	3.14486270422678	bayes_pls_golite062009
catalytic activity	2.29878871754893	bayes_pls_golite062009
transferase activity	1.44093490824786	bayes_pls_golite062009
transferase activity, transferring nitrogenous groups	1.28316822767388	bayes_pls_golite062009
transaminase activity	1.24990480711889	bayes_pls_golite062009
methyltransferase activity	0.681623261233707	bayes_pls_golite062009
L-aspartate:2-oxoglutarate aminotransferase activity	0.619695949258279	bayes_pls_golite062009
transferase activity, transferring one-carbon groups	0.60304280865376	bayes_pls_golite062009
glycine hydroxymethyltransferase activity	0.271623203801431	bayes_pls_golite062009
O-phospho-L-serine:2-oxoglutarate aminotransferase activity	0.234399054481664	bayes_pls_golite062009
pyridoxal phosphate binding	0.16957228630576	bayes_pls_golite062009
vitamin B6 binding	0.16957228630576	bayes_pls_golite062009

Term	Confidence	Notes
glycine dehydrogenase (decarboxylating) activity	8.00572280567771	bayes_pls_golite062009
oxidoreductase activity, acting on the CH-NH2 group of donors, disulfide as acceptor	8.00572280567771	bayes_pls_golite062009
oxidoreductase activity, acting on the CH-NH2 group of donors	3.14486270422678	bayes_pls_golite062009
catalytic activity	2.29878871754893	bayes_pls_golite062009
transferase activity	1.44093490824786	bayes_pls_golite062009
transferase activity, transferring nitrogenous groups	1.28316822767388	bayes_pls_golite062009
transaminase activity	1.24990480711889	bayes_pls_golite062009
methyltransferase activity	0.681623261233707	bayes_pls_golite062009
L-aspartate:2-oxoglutarate aminotransferase activity	0.619695949258279	bayes_pls_golite062009
transferase activity, transferring one-carbon groups	0.60304280865376	bayes_pls_golite062009
glycine hydroxymethyltransferase activity	0.271623203801431	bayes_pls_golite062009
O-phospho-L-serine:2-oxoglutarate aminotransferase activity	0.234399054481664	bayes_pls_golite062009
pyridoxal phosphate binding	0.16957228630576	bayes_pls_golite062009
vitamin B6 binding	0.16957228630576	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

[View Details]

Source: Reynolds et al. 2008. Manuscript submitted

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2