Protein Overview: PAH1

General Information:
Name(s) found:	PAH1 / YMR165C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|825570\|emb\|CAA89801.1\| Smp2p [Saccharomyces cerevisiae] [NCBI NR] gi\|6323817\|ref\|NP_013888.1\| Mg2+-dependent phosphatidate (PA) phosphatase, catalyzes the dephosphorylation of PA to yield diacylglycerol and Pi, responsible for de novo lipid synthesis; homologous to mammalian lipin 1; Pah1p [Saccharomyces cerevisiae] [NCBI NR] gi\|445061\|prf\|\|1908378A SMP2 gene [NCBI NR] gi\|417782\|sp\|P32567.1\|SMP2_YEAST RecName: Full=Protein SMP2 [NCBI NR] pir\|\|S30911 SMP2 protein - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|218488\|dbj\|BAA00880.1\| Smp2 protein [Saccharomyces cerevisiae] [NCBI NR] Protein SMP2 OS=Saccharomyces cerevisiae GN=SMP2 PE=1 SV=1 [Swiss-Prot] SMP2 Protein involved in plasmid maintenance, respiration and cell proliferation [mips] Mg<sup>2+</sup>-dependent phosphatidate (PA) phosphatase, catalyzes the dephosphorylation of PA to yield diacylglycerol and P<sub>i</sub>, responsible for de novo lipid synthesis; homologous to mammalian lipin 1 [SGD] SMP2, Chr XIII from 590039-592627, reverse complement [yeast_orfs2] Chr XIII from 590039-592627, reverse complement [yeastorf3.fasta] Chr XIII from 592627-590039, reverse complement [yeastorf5.fasta] (P32567) SMP2 protein [4932.FASTACCERV.txt] SMP2 SGDID:S0004775, Chr XIII from 592627-590039, reverse complement, Verified ORF [yeastflysequest.fasta] Protein involved in respiration and plasmid maintenance [Saccharomyces cerevisiae]gi\|825570\|emb\|CAA89801.1\| Smp2p [Saccharomyces cerevisiae]gi\|417782\|sp\|P32567\|SMP2_YEAST SMP2 proteingi\|320853\|pir\|\|S30911 SMP2 protein - yeast (Saccharomyces cerevisiae) [nrdb_11152004_Chlamydomonas] SMP2 SGDID:S000004775, Chr XIII from 592627-590039, reverse complement, Verified ORF, "Protein of unknown function involved in respiration and plasmid maintenance; proposed to be involved in cell wall integrity" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] PAH1 SGDID:S000004775, Chr XIII from 592627-590039, reverse complement, Verified ORF, "Mg<sup>2+</sup>-dependent phosphatidate (PA) phosphatase, catalyzes the dephosphorylation of PA to yield diacylglycerol and P<sub>i</sub>, responsible for de novo lipid [yeast-contam.fasta] Mg2+-dependent phosphatidate (PA) phosphatase, catalyzes the dephosphorylation of PA to yield diacylglycerol and Pi, responsible for de novo lipid synthesis; homologous to mammalian lipin 1; Pah1p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Smp2p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Smp2 protein [Saccharomyces cerevisiae] [nr-271106-contam.fasta] SMP2 gene [nr-271106-contam.fasta] Protein SMP2 [nr-271106-contam.fasta] PAH1 SGDID:S000004775, Chr XIII from 592627-590039, reverse complement, Verified ORF, "Mg<sup>2+</sup>-dependent phosphatidate (PA) phosphatase, catalyzes the dephosphorylation of PA to yield diacylglycerol and P<sub>i</sub>, responsible for de novo lipid synthesis; homologous to mammalian lipin 1" [yeast-bovrabprots.fasta] [chemostatdb.fasta] PAH1 SGDID:S000004775, Chr XIII from 592628-590040, Genome Release 64-1-1, reverse complement, Verified ORF, "Mg<sup>2+</sup>-dependent phosphatidate (PA) phosphatase, catalyzes the dephosphorylation of PA to yield diacylglycerol, responsible for de novo lipid synthesis and formation of lipid droplets; homologous to mammalian lipin 1" [yeast-030211-contam.fasta] PAH1 SGDID:S000004775, Chr XIII from 592628-590040, Genome Release 64-2-1, reverse complement, Verified ORF, "Mg2+-dependent phosphatidate (PA) phosphatase; dephosphorylates PA to yield diacylglycerol; responsible for de novo lipid synthesis and formation of lipid droplets; phosphorylation by Pho80p-Pho85p decreases catalytic activity and alters Pah1p localization and abundance; phosphorylation by protein kinase A decreases catalytic efficiency; dephosphorylation by Nem1p-Spo7p anchors Pah1p to the membrane increasing substrate catalysis; homologous to mammalian lipins 1 and 2" [20141208_orf_trans.fasta] YMR165C SGDID:S000004775, Chr XIII from 592628-590040, Genome Release 64-1-1, reverse complement, Verified ORF, "Mg<sup>2+</sup>-dependent phosphatidate (PA) phosphatase, catalyzes the dephosphorylation of PA to yield diacylglycerol, responsible for de novo lipid synthesis and formation of lipid droplets; homologous to mammalian lipin 1" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	862 amino acids
Gene Ontology:
Cellular Component:	membrane fraction [IDA] cytoplasm [IDA]
Biological Process:	lipid biosynthetic process [IMP] plasmid maintenance [IMP] aerobic respiration [IMP]
Molecular Function:	phosphatidate phosphatase activity [IDA][IMP]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MQYVGRALGS VSKTWSSINP ATLSGAIDVI VVEHPDGRLS CSPFHVRFGK FQILKPSQKK  60
   61 VQVFINEKLS NMPMKLSDSG EAYFVFEMGD QVTDVPDELL VSPVMSATSS PPQSPETSIL 120
  121 EGGTEGEGEG ENENKKKEKK VLEEPDFLDI NDTGDSGSKN SETTGSLSPT ESSTTTPPDS 180
  181 VEERKLVEQR TKNFQQKLNK KLTEIHIPSK LDNNGDLLLD TEGYKPNKNM MHDTDIQLKQ 240
  241 LLKDEFGNDS DISSFIKEDK NGNIKIVNPY EHLTDLSPPG TPPTMATSGS VLGLDAMESG 300
  301 STLNSLSSSP SGSDTEDETS FSKEQSSKSE KTSKKGTAGS GETEKRYIRT IRLTNDQLKC 360
  361 LNLTYGENDL KFSVDHGKAI VTSKLFVWRW DVPIVISDID GTITKSDALG HVLAMIGKDW 420
  421 THLGVAKLFS EISRNGYNIL YLTARSAGQA DSTRSYLRSI EQNGSKLPNG PVILSPDRTM 480
  481 AALRREVILK KPEVFKIACL NDIRSLYFED SDNEVDTEEK STPFFAGFGN RITDALSYRT 540
  541 VGIPSSRIFT INTEGEVHME LLELAGYRSS YIHINELVDH FFPPVSLDSV DLRTNTSMVP 600
  601 GSPPNRTLDN FDSEITSGRK TLFRGNQEEK FTDVNFWRDP LVDIDNLSDI SNDDSDNIDE 660
  661 DTDVSQQSNI SRNRANSVKT AKVTKAPQRN VSGSTNNNEV LAASSDVENA SDLVSSHSSS 720
  721 GSTPNKSTMS KGDIGKQIYL ELGSPLASPK LRYLDDMDDE DSNYNRTKSR RASSAAATSI 780
  781 DKEFKKLSVS KAGAPTRIVS KINVSNDVHS LGNSDTESRR EQSVNETGRN QLPHNSMDDK 840
  841 DLDSRVSDEF DDDEFDEDEF ED

Protein Complex Data

No complex found for this protein.

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	CEP3	sample cbf3 from feb 2005	Sandall S, et all (2006)
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	EST1	PAH1	1	Unpublished Fields Lab Data
View Screen	BUB3	PAH1	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	PAH1	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..156]	deduced	N/A	No confident structure predictions are available.
2	View Details	[157..654]	MSA	4.01789	View MSA. No confident structure predictions are available.
3	View Details	[655..862]	deduced	N/A	No confident structure predictions are available.
4	View Details	[657..862]	deduced	N/A	No confident structure predictions are available.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.96

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2