Protein Overview: IXR1

General Information:
Name(s) found:	IXR1 / YKL032C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6322820\|ref\|NP_012893.1\| Protein that binds DNA containing intrastrand cross-links formed by cisplatin, contains two HMG (high mobility group box) domains, which confer the ability to bend cisplatin-modified DNA; mediates aerobic transcriptional repres [NCBI NR] gi\|547746\|sp\|P33417.2\|IXR1_YEAST RecName: Full=Intrastrand cross-link recognition protein; AltName: Full=Structure-specific recognition protein; Short=SSRP [NCBI NR] pir\|\|S37849 DNA intrastrand crosslink recognition protein - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|486034\|emb\|CAA81867.1\| IXR1 [Saccharomyces cerevisiae] [NCBI NR] gi\|4467991\|emb\|CAB37853.1\| unnamed protein product [Saccharomyces cerevisiae] [NCBI NR] Intrastrand cross-link recognition protein OS=Saccharomyces cerevisiae GN=IXR1 PE=1 SV=2 [Swiss-Prot] IXR1 Intrastrand crosslink recognition protein and transcription factor [mips] Protein that binds DNA containing intrastrand cross-links formed by cisplatin, contains two HMG (high mobility group box) domains, which confer the ability to bend cisplatin-modified DNA; mediates aerobic transcriptional repression of COX5b [SGD] IXR1, Chr XI from 379703-381496, reverse complement [yeast_orfs2] Chr XI from 379703-381496, reverse complement [yeastorf3.fasta] Chr XI from 381496-379703, reverse complement [yeastorf5.fasta] (P33417) Intrastrand crosslink recognition protein (Structure-specific recognition protein) (SSRP) [4932.FASTACCERV.txt] IXR1 SGDID:S0001515, Chr XI from 381501-379708, reverse complement, Verified ORF [yeastflysequest.fasta] Ixr1p [Saccharomyces cerevisiae]gi\|486034\|emb\|CAA81867.1\| IXR1 [Saccharomyces cerevisiae]gi\|4467991\|emb\|CAB37853.1\| unnamed protein product [Saccharomyces cerevisiae]gi\|539104\|pir\|\|S37849 DNA intrastrand crosslink recognition protein - yeast (Saccharo [nrdb_11152004_Chlamydomonas] IXR1 SGDID:S000001515, Chr XI from 381501-379708, reverse complement, Verified ORF, "Protein that binds DNA containing intrastrand cross-links formed by cisplatin, contains two HMG (high mobility group box) domains, which confer the ability to bend cispla [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] unnamed protein product [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Protein that binds DNA containing intrastrand cross-links formed by cisplatin, contains two HMG (high mobility group box) domains, which confer the ability to bend cisplatin-modified DNA; mediates aerobic transcriptional repression of COX5b; Ixr1p [Saccha [nr-271106-contam.fasta] IXR1 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Intrastrand cross-link recognition protein (Structure-specific recognition protein) (SSRP) [nr-271106-contam.fasta] IXR1 SGDID:S000001515, Chr XI from 381501-379708, reverse complement, Verified ORF, "Protein that binds DNA containing intrastrand cross-links formed by cisplatin, contains two HMG (high mobility group box) domains, which confer the ability to bend cisplatin-modified DNA; mediates aerobic transcriptional repression of COX5b" [yeast-bovrabprots.fasta] [chemostatdb.fasta] Intrastrand cross-link recognition protein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=IXR1 PE=1 SV=2 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] IXR1 SGDID:S000001515, Chr XI from 381858-380065, Genome Release 64-1-1, reverse complement, Verified ORF, "Protein that binds DNA containing intrastrand cross-links formed by cisplatin, contains two HMG (high mobility group box) domains, which confer the ability to bend cisplatin-modified DNA; mediates aerobic transcriptional repression of COX5b" [yeast-030211-contam.fasta] IXR1 SGDID:S000001515, Chr XI from 381858-380065, Genome Release 64-2-1, reverse complement, Verified ORF, "Transcriptional repressor that regulates hypoxic genes during normoxia; involved in the aerobic repression of genes such as COX5b, TIR1, and HEM13; binds DNA intrastrand cross-links formed by cisplatin; HMG (high mobility group box) domain containing protein which binds and bends cisplatin-modified DNA, blocking excision repair; IXR1 has a paralog, ABF2, that arose from the whole genome duplication" [20141208_orf_trans.fasta] YKL032C SGDID:S000001515, Chr XI from 381858-380065, Genome Release 64-1-1, reverse complement, Verified ORF, "Protein that binds DNA containing intrastrand cross-links formed by cisplatin, contains two HMG (high mobility group box) domains, which confer the ability to bend cisplatin-modified DNA; mediates aerobic transcriptional repression of COX5b" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	597 amino acids
Gene Ontology:
Cellular Component:	nuclear chromosome [IPI]
Biological Process:	DNA repair [IMP]
Molecular Function:	DNA binding [IDA]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MNTGISPKQD DASNSNLLNI GQDHSLQYQG LEHNDSQYRD ASHQTPHQYL NQFQAQPQQQ  60
   61 QQQQQQQQQQ QQQAPYQGHF QQSPQQQQQN VYYPLPPQSL TQPTSQSQQQ QQQQQQQQYA 120
  121 NSNSNSNNNV NVNALPQDFG YMQQTGSGQN YPTINQQQFS EFYNSFLSHL TQKQTNPSVT 180
  181 GTGASSNNNS NNNNVSSGNN STSSNPAQLA ASQLNPATAA AAAANNAAGP ASYLSQLPQV 240
  241 QRYYPNNMNA LSSLLDPSSA GNAAGNANTA THPGLLPPNL QPQLTHHQQQ MQQQLQLQQQ 300
  301 QQLQQQQQLQ QQHQLQQQQQ LQQQHHHLQQ QQQQQQHPVV KKLSSTQSRI ERRKQLKKQG 360
  361 PKRPSSAYFL FSMSIRNELL QQFPEAKVPE LSKLASARWK ELTDDQKKPF YEEFRTNWEK 420
  421 YRVVRDAYEK TLPPKRPSGP FIQFTQEIRP TVVKENPDKG LIEITKIIGE RWRELDPAKK 480
  481 AEYTETYKKR LKEWESCYPD ENDPNGNPTG HSHKAMNMNL NMDTKIMENQ DSIEHITANA 540
  541 IDSVTGSNSN STNPNTPVSP PISLQQQPLQ QQQQQQQQQQ HMLLADPTTN GSIIKNE

Protein Complex Data

No complex found for this protein.

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)
View Run	MLP2	#26 Asynchronous Prep4-TiO2 Phosphopeptide enriched, Steps1-2	Keck JM, et al. (2011)
View Run	MLP2	#22 Asynchronous Prep2-IMAC Phosphopeptide enrichment B1-2	Keck JM, et al. (2011)
View Run	MLP2	#13 Mitotic Prep2-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

No yeast two-hybrid interactions found for this protein.

	PROTEIN(S)	PUBLICATION
View Data	IXR1	Huh WK, et al. (2003)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..121]	MSA	1.017991	View MSA. No confident structure predictions are available.
2	View Details	[122..173]	deduced	N/A	No confident structure predictions are available.
3	View Details	[174..336]	PSI-BLAST	39.071898	Upstream binding factor
4	View Details	[337..427]	PSI-BLAST	58.045757	Sox-5
5	View Details	[428..511]	PSI-BLAST	41.29073	NHP6a
6	View Details	[512..597]	MSA	1.177993	View MSA. No confident structure predictions are available.

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Protein Overview: IXR1

Protein Complex Data

Mass Spectrometry Data

Yeast Two-Hybrid Data

Microscopy / Localization Data

Protein Structure Data

Philius Transmembrane Prediction: