Protein Overview: EXO70

General Information:
Name(s) found:	EXO70 / YJL085W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|929871\|emb\|CAA58485.1\| J0932/ORF2 [Saccharomyces cerevisiae] [NCBI NR] pir\|\|A27300 hypothetical protein YJL085w - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|6322375\|ref\|NP_012450.1\| Essential 70kDa subunit of the exocyst complex (Sec3p, Sec5p, Sec6p, Sec8p, Sec10p, Sec15p, Exo70p, and Exo84p), which has the essential function of mediating polarized targeting of secretory vesicles to active sites of exocyto [NCBI NR] gi\|4090\|emb\|CAA68535.1\| unnamed protein product [Saccharomyces cerevisiae] [NCBI NR] gi\|1781303\|emb\|CAA70039.1\| 70 kD Exocyst complex protein [Saccharomyces cerevisiae] [NCBI NR] gi\|141470\|sp\|P19658.1\|EXO70_YEAST RecName: Full=Exocyst complex component EXO70; AltName: Full=Exocyst complex protein of 70 kDa [NCBI NR] gi\|1008254\|emb\|CAA89380.1\| EXO70 [Saccharomyces cerevisiae] [NCBI NR] Exocyst complex component EXO70 OS=Saccharomyces cerevisiae GN=EXO70 PE=1 SV=1 [Swiss-Prot] (P19658) 70 kDa exocyst complex protein [Swiss-Prot] EXO70 70 kDa exocyst component protein [mips] Subunit of the exocyst complex (Sec3p, Sec5p, Sec6p, Sec8p, Sec10p, Sec15p, Exo70p, and Exo84p), which directs secretory vesicles to active sites of exocytosis; acts with Sec3p in membrane targeting of the exocyst via PI(4,5)P2 binding [SGD] EXO70, Chr X from 272523-274394 [yeast_orfs2] Chr X from 272523-274394 [yeastorf3.fasta] EXO70 SGDID:S0003621, Chr X from 272743-274614, Verified ORF [yeastflysequest.fasta] Essential 70kDa subunit of the exocyst complex (Sec3p, Sec5p, Sec6p, Sec8p, Sec10p, Sec15p, Exo70p, and Exo84p), which has the essential function of mediating polarized targeting of secretory vesicles to active sites of exocytosis [Saccharomyces cerevisia [nrdb_11152004_Chlamydomonas] EXO70 SGDID:S000003621, Chr X from 272743-274614, Verified ORF, "Essential 70kDa subunit of the exocyst complex (Sec3p, Sec5p, Sec6p, Sec8p, Sec10p, Sec15p, Exo70p, and Exo84p), which has the essential function of mediating polarized targeting of secretor [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] EXO70 SGDID:S000003621, Chr X from 272822-274693, Verified ORF, "Essential 70kDa subunit of the exocyst complex (Sec3p, Sec5p, Sec6p, Sec8p, Sec10p, Sec15p, Exo70p, and Exo84p), which has the essential function of mediating polarized targeting of secretor [yeast-contam.fasta] EXO70 SGDID:S000003621, Chr X from 272821-274692, Verified ORF, "Subunit of the exocyst complex (Sec3p, Sec5p, Sec6p, Sec8p, Sec10p, Sec15p, Exo70p, and Exo84p), which directs secretory vesicles to active sites of exocytosis; acts with Sec3p in membrane t [yeast-200209-contam.fasta] unnamed protein product [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Essential 70kDa subunit of the exocyst complex (Sec3p, Sec5p, Sec6p, Sec8p, Sec10p, Sec15p, Exo70p, and Exo84p), which has the essential function of mediating polarized targeting of secretory vesicles to active sites of exocytosis; Exo70p [Saccharomyces c [nr-271106-contam.fasta] J0932/ORF2 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Exocyst complex component EXO70 (Exocyst complex protein of 70 kDa) [nr-271106-contam.fasta] 70 kD Exocyst complex protein [Saccharomyces cerevisiae] [nr-271106-contam.fasta] EXO70 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] EXO70 SGDID:S000003621, Chr X from 272821-274692, Verified ORF, "Subunit of the exocyst complex (Sec3p, Sec5p, Sec6p, Sec8p, Sec10p, Sec15p, Exo70p, and Exo84p), which directs secretory vesicles to active sites of exocytosis; acts with Sec3p in membrane targeting of the exocyst via PI(4,5)P2 binding" [yeast-bovrabprots.fasta] EXO70 SGDID:S000003621, Chr X from 272822-274693, Verified ORF, "Essential 70kDa subunit of the exocyst complex (Sec3p, Sec5p, Sec6p, Sec8p, Sec10p, Sec15p, Exo70p, and Exo84p), which has the essential function of mediating polarized targeting of secretory vesicles to active sites of exocytosis" [yeast-contam_custom.fasta] [chemostatdb.fasta] Exocyst complex component EXO70 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=EXO70 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] EXO70 SGDID:S000003621, Chr X from 272827-274698, Genome Release 64-1-1, Verified ORF, "Subunit of the exocyst complex (Sec3p, Sec5p, Sec6p, Sec8p, Sec10p, Sec15p, Exo70p, and Exo84p), which directs secretory vesicles to active sites of exocytosis; acts with Sec3p in membrane targeting of the exocyst via PI(4,5)P2 binding" [yeast-030211-contam.fasta] EXO70 SGDID:S000003621, Chr X from 272827-274698, Genome Release 64-2-1, Verified ORF, "Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in an actin-independent manner, targeting and anchoring the exocyst with Sec3p; involved in exocyst assembly; direct downstream effector of Rho3p and Cdc42p; relocalizes from bud neck to cytoplasm upon DNA replication stress" [20141208_orf_trans.fasta] YJL085W SGDID:S000003621, Chr X from 272827-274698, Genome Release 64-1-1, Verified ORF, "Subunit of the exocyst complex (Sec3p, Sec5p, Sec6p, Sec8p, Sec10p, Sec15p, Exo70p, and Exo84p), which directs secretory vesicles to active sites of exocytosis; acts with Sec3p in membrane targeting of the exocyst via PI(4,5)P2 binding" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	623 amino acids
Gene Ontology:
Cellular Component:	exocyst [IPI]
Biological Process:	vesicle fusion [TAS] bipolar cellular bud site selection [IMP] vesicle docking during exocytosis [TAS] Golgi to plasma membrane transport [TAS] cytokinesis [IMP]
Molecular Function:	protein binding [IPI]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MPAEIDIDEA DVLVLSQELQ KTSKLTFEIN KSLKKIAATS NQSSQLFTPI LARNNVLTTL  60
   61 QRNIESTLNS VASVKDLANE ASKYEIILQK GINQVGLKQY TQVVHKLDDM LEDIQSGQAN 120
  121 REENSEFHGI LTHLEQLIKR SEAQLRVYFI SILNSIKPFD PQINITKKMP FPYYEDQQLG 180
  181 ALSWILDYFH GNSEGSIIQD ILVGERSKLI LKCMAFLEPF AKEISTAKNA PYEKGSSGMN 240
  241 SYTEALLGFI ANEKSLVDDL YSQYTESKPH VLSQILSPLI SAYAKLFGAN LKIVRSNLEN 300
  301 FGFFSFELVE SINDVKKSLR GKELQNYNLL QDCTQEVRQV TQSLFRDAID RIIKKANSIS 360
  361 TIPSNNGVTE ATVDTMSRLR KFSEYKNGCL GAMDNITREN WLPSNYKEKE YTLQNEALNW 420
  421 EDHNVLLSCF ISDCIDTLAV NLERKAQIAL MPNQEPDVAN PNSSKNKHKQ RIGFFILMNL 480
  481 TLVEQIVEKS ELNLMLAGEG HSRLERLKKR YISYMVSDWR DLTANLMDSV FIDSSGKKSK 540
  541 DKEQIKEKFR KFNEGFEDLV SKTKQYKLSD PSLKVTLKSE IISLVMPMYE RFYSRYKDSF 600
  601 KNPRKHIKYT PDELTTVLNQ LVR

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	(MIPS) Mewes HW, et al. (2004)		EXO70 SEC10 SEC15 SEC3 SEC5 SEC6 SEC8
View Details	Ho Y, et al. (2002)		CIN5 EXO70 HHF1, HHF2 HTA1 IRC20
View Details	Qiu et al. (2008)		EXO70 EXO84 SEC10 SEC15 SEC3 SEC5 SEC6 SEC8

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	SEC10	No Comments	De Craene, J., et al. (2006)
View Run	ZDS1	Sample 2- cpn2 from june 2005	McCusker D, et al (2007)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	MET18	EXO70	1	Unpublished Fields Lab Data
View Screen	RRB1	EXO70	1	Unpublished Fields Lab Data
View Screen	YMR178W	EXO70	1	Unpublished Fields Lab Data

Microscopy / Localization Data

New Feature: Upload Your Own Microscopy Data

	PROTEIN(S)	PUBLICATION
View Data	EXO70	Huh WK, et al. (2003)

Protein Structure Data

[What does the above image mean?]
[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..216]	ORFEUS	8.48	alpha-actinin
2	View Details	[217..533]	MSA	1.038953	View MSA. No confident structure predictions are available.
3	View Details	[534..623]	deduced	N/A	No confident structure predictions are available.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2