Protein Overview: POT1

General Information:
Name(s) found:	POT1 / YIL160C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6322031\|ref\|NP_012106.1\| 3-ketoacyl-CoA thiolase with broad chain length specificity, cleaves 3-ketoacyl-CoA into acyl-CoA and acetyl-CoA during beta-oxidation of fatty acids; Pot1p [Saccharomyces cerevisiae] [NCBI NR] gi\|557763\|emb\|CAA86118.1\| 3-ketoacyl-coA thiolase [Saccharomyces cerevisiae] [NCBI NR] gi\|51013819\|gb\|AAT93203.1\| YIL160C [Saccharomyces cerevisiae] [NCBI NR] gi\|4197\|emb\|CAA37472.1\| 3-oxoacyl thiolase peroxisomal [Saccharomyces cerevisiae] [NCBI NR] gi\|3702\|emb\|CAA37893.1\| 3-oxoacyl-CoA thiolase [Saccharomyces cerevisiae] [NCBI NR] gi\|135753\|sp\|P27796.1\|THIK_YEAST RecName: Full=3-ketoacyl-CoA thiolase, peroxisomal; AltName: Full=Beta-ketothiolase; AltName: Full=Acetyl-CoA acyltransferase; AltName: Full=Peroxisomal 3-oxoacyl-CoA thiolase; Flags: Precursor [NCBI NR] pir\|\|S22784 acetyl-CoA C-acyltransferase (EC 2.3.1.16), peroxisomal - yeast (Saccharomyces cerevisiae) [NCBI NR] 3-ketoacyl-CoA thiolase, peroxisomal OS=Saccharomyces cerevisiae GN=FOX3 PE=1 SV=1 [Swiss-Prot] POT1 Acetyl-CoA C-acyltransferase, peroxisomal [mips] 3-ketoacyl-CoA thiolase with broad chain length specificity, cleaves 3-ketoacyl-CoA into acyl-CoA and acetyl-CoA during beta-oxidation of fatty acids [SGD] POT1, Chr IX from 40191-41444, reverse complement [yeast_orfs2] Chr IX from 40191-41444, reverse complement [yeastorf3.fasta] Chr IX from 41444-40191, reverse complement [yeastorf5.fasta] (P27796) 3-ketoacyl-CoA thiolase, peroxisomal precursor (EC 2.3.1.16) (Beta-ketothiolase) (Acetyl-CoA acyltransferase) (Peroxisomal 3-oxoacyl-CoA thiolase) [4932.FASTACCERV.txt] POT1 SGDID:S0001422, Chr IX from 41444-40191, reverse complement, Verified ORF [yeastflysequest.fasta] 3-ketoacyl-CoA thiolase with broad chain length specifity, cleaves 3-ketoacyl-CoA into acyl-CoA and acetyl-CoA during beta-oxidation of fatty acids [Saccharomyces cerevisiae]gi\|51013819\|gb\|AAT93203.1\| YIL160C [Saccharomyces cerevisiae]gi\|3702\|emb\|CAA378 [nrdb_11152004_Chlamydomonas] POT1 SGDID:S000001422, Chr IX from 41444-40191, reverse complement, Verified ORF, "3-ketoacyl-CoA thiolase with broad chain length specificity, cleaves 3-ketoacyl-CoA into acyl-CoA and acetyl-CoA during beta-oxidation of fatty acids" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] 3-ketoacyl-CoA thiolase with broad chain length specificity, cleaves 3-ketoacyl-CoA into acyl-CoA and acetyl-CoA during beta-oxidation of fatty acids; Pot1p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] 3-oxoacyl thiolase peroxisomal [Saccharomyces cerevisiae] [nr-271106-contam.fasta] 3-ketoacyl-coA thiolase [Saccharomyces cerevisiae] [nr-271106-contam.fasta] 3-ketoacyl-CoA thiolase, peroxisomal precursor (Beta-ketothiolase) (Acetyl-CoA acyltransferase) (Peroxisomal 3-oxoacyl-CoA thiolase) [nr-271106-contam.fasta] 3-oxoacyl-CoA thiolase [Saccharomyces cerevisiae] [nr-271106-contam.fasta] YIL160C [Saccharomyces cerevisiae] [nr-271106-contam.fasta] [chemostatdb.fasta] 3-ketoacyl-CoA thiolase, peroxisomal OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=POT1 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] POT1 SGDID:S000001422, Chr IX from 41444-40191, Genome Release 64-1-1, reverse complement, Verified ORF, "3-ketoacyl-CoA thiolase with broad chain length specificity, cleaves 3-ketoacyl-CoA into acyl-CoA and acetyl-CoA during beta-oxidation of fatty acids" [yeast-030211-contam.fasta] POT1 SGDID:S000001422, Chr IX from 41444-40191, Genome Release 64-2-1, reverse complement, Verified ORF, "3-ketoacyl-CoA thiolase with broad chain length specificity; cleaves 3-ketoacyl-CoA into acyl-CoA and acetyl-CoA during beta-oxidation of fatty acids" [20141208_orf_trans.fasta] YIL160C SGDID:S000001422, Chr IX from 41444-40191, Genome Release 64-1-1, reverse complement, Verified ORF, "3-ketoacyl-CoA thiolase with broad chain length specificity, cleaves 3-ketoacyl-CoA into acyl-CoA and acetyl-CoA during beta-oxidation of fatty acids" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	417 amino acids
Gene Ontology:
Cellular Component:	peroxisomal matrix [TAS]
Biological Process:	fatty acid beta-oxidation [TAS]
Molecular Function:	acetyl-CoA C-acyltransferase activity [TAS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSQRLQSIKD HLVESAMGKG ESKRKNSLLE KRPEDVVIVA ANRSAIGKGF KGAFKDVNTD  60
   61 YLLYNFLNEF IGRFPEPLRA DLNLIEEVAC GNVLNVGAGA TEHRAACLAS GIPYSTPFVA 120
  121 LNRQCSSGLT AVNDIANKIK VGQIDIGLAL GVESMTNNYK NVNPLGMISS EELQKNREAK 180
  181 KCLIPMGITN ENVAANFKIS RKDQDEFAAN SYQKAYKAKN EGLFEDEILP IKLPDGSICQ 240
  241 SDEGPRPNVT AESLSSIRPA FIKDRGTTTA GNASQVSDGV AGVLLARRSV ANQLNLPVLG 300
  301 RYIDFQTVGV PPEIMGVGPA YAIPKVLEAT GLQVQDIDIF EINEAFAAQA LYCIHKLGID 360
  361 LNKVNPRGGA IALGHPLGCT GARQVATILR ELKKDQIGVV SMCIGTGMGA AAIFIKE

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Qiu et al. (2008)		CAT2 DCI1 ECI1 FOX2 MLS1 POT1 POX1

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)
View Run	MLP2	#12 Mitotic Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#11 Mitotic Prep1-TiO2 enriched, new search criteria	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	LIA1	POT1	1	Unpublished Fields Lab Data
View Screen	YLR224W	POT1	1	Unpublished Fields Lab Data
View Screen	NOP6	POT1	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	POT1	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..417]	PSI-BLAST	10122.0	Thiolase

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
acetyl-CoA C-acyltransferase activity	8.08119341370748	bayes_pls_golite062009
long-chain-3-hydroxyacyl-CoA dehydrogenase activity	7.45371477074071	bayes_pls_golite062009
long-chain-enoyl-CoA hydratase activity	5.63866392580915	bayes_pls_golite062009
transferase activity, transferring acyl groups	4.96408928512745	bayes_pls_golite062009
fatty acid synthase activity	3.79121338118181	bayes_pls_golite062009
3-oxoacyl-[acyl-carrier-protein] synthase activity	3.71249191203055	bayes_pls_golite062009
C-acyltransferase activity	3.27532895419051	bayes_pls_golite062009
transferase activity, transferring acyl groups other than amino-acyl groups	3.14957464437124	bayes_pls_golite062009
acyltransferase activity	3.06879684191353	bayes_pls_golite062009
transferase activity	2.15834234036431	bayes_pls_golite062009
catalytic activity	2.14505178497871	bayes_pls_golite062009
3-hydroxyacyl-CoA dehydrogenase activity	1.87304265492939	bayes_pls_golite062009
transferase activity, transferring acyl groups, acyl groups converted into alkyl on transfer	1.77798276950874	bayes_pls_golite062009
acetyl-CoA C-acetyltransferase activity	1.50839304016514	bayes_pls_golite062009
C-acetyltransferase activity	1.19264084089277	bayes_pls_golite062009
hydroxymethylglutaryl-CoA synthase activity	1.16785899347652	bayes_pls_golite062009
acetyltransferase activity	0.6519546920419	bayes_pls_golite062009
binding	0.415385255550741	bayes_pls_golite062009
enoyl-CoA hydratase activity	0.21899828831708	bayes_pls_golite062009

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.48

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2