Protein Overview: GIC1

General Information:
Name(s) found:	GIC1 / YHR061C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|731663\|sp\|P38785.1\|GIC1_YEAST RecName: Full=GTPase-interacting component 1 [NCBI NR] gi\|6321852\|ref\|NP_011928.1\| Protein of unknown function involved in initiation of budding and cellular polarization, interacts with Cdc42p via the Cdc42/Rac-interactive binding (CRIB) domain; Gic1p [Saccharomyces cerevisiae] [NCBI NR] pir\|\|S46709 hypothetical protein YHR061c - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|487957\|gb\|AAB68388.1\| Yhr061cp [Saccharomyces cerevisiae] [NCBI NR] GTPase-interacting component 1 OS=Saccharomyces cerevisiae GN=GIC1 PE=1 SV=1 [Swiss-Prot] GIC1 Cdc42 GTPase-binding protein [mips] Protein of unknown function involved in initiation of budding and cellular polarization, interacts with Cdc42p via the Cdc42/Rac-interactive binding (CRIB) domain [SGD] GIC1, Chr VIII from 221535-222479, reverse complement [yeast_orfs2] Chr VIII from 221535-222479, reverse complement [yeastorf3.fasta] Chr VIII from 222479-221535, reverse complement [yeastorf5.fasta] (P38785) GTPase-interacting component 1 [4932.FASTACCERV.txt] GIC1 SGDID:S0001103, Chr VIII from 222479-221535, reverse complement, Verified ORF [yeastflysequest.fasta] Gic1p [Saccharomyces cerevisiae]gi\|731663\|sp\|P38785\|GIC1_YEAST GTPase-interacting component 1gi\|626620\|pir\|\|S46709 hypothetical protein YHR061c - yeast (Saccharomyces cerevisiae)gi\|487957\|gb\|AAB68388.1\| Yhr061cp [Saccharomyces cerevisiae] [nrdb_11152004_Chlamydomonas] GIC1 SGDID:S000001103, Chr VIII from 222480-221536, reverse complement, Verified ORF, "Protein of unknown function involved in initiation of budding and cellular polarization, interacts with Cdc42p via the Cdc42/Rac-interactive binding (CRIB) domain" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] Yhr061cp [Saccharomyces cerevisiae] [nr-271106-contam.fasta] GTPase-interacting component 1 [nr-271106-contam.fasta] Protein of unknown function involved in initiation of budding and cellular polarization, interacts with Cdc42p via the Cdc42/Rac-interactive binding (CRIB) domain; Gic1p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] [chemostatdb.fasta] GTPase-interacting component 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=GIC1 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] GIC1 SGDID:S000001103, Chr VIII from 222478-221534, Genome Release 64-1-1, reverse complement, Verified ORF, "Protein of unknown function involved in initiation of budding and cellular polarization, interacts with Cdc42p via the Cdc42/Rac-interactive binding (CRIB) domain" [yeast-030211-contam.fasta] GIC1 SGDID:S000001103, Chr VIII from 222478-221534, Genome Release 64-2-1, reverse complement, Verified ORF, "Protein involved in initiation of budding and cellular polarization; interacts with Cdc42p via the Cdc42/Rac-interactive binding (CRIB) domain; relocalizes from bud neck to nucleus upon DNA replication stress; GIC1 has a paralog, GIC2, that arose from the whole genome duplication" [20141208_orf_trans.fasta] TR467\|c0_g1_i1\|g.247 ORF TR467\|c0_g1_i1\|g.247 TR467\|c0_g1_i1\|m.247 type:complete len:315 (+) TR467\|c0_g1_i1:200-1144(+) [Y12_S_cerevisiae-Sep2015-contam.fasta] YHR061C SGDID:S000001103, Chr VIII from 222478-221534, Genome Release 64-1-1, reverse complement, Verified ORF, "Protein of unknown function involved in initiation of budding and cellular polarization, interacts with Cdc42p via the Cdc42/Rac-interactive binding (CRIB) domain" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta] asmbl_7973\|g.5807 ORF asmbl_7973\|g.5807 asmbl_7973\|m.5807 type:complete len:315 (+) asmbl_7973:219-1163(+) [Y12_Trinity_v2_2_0_PASA_6May2016-contam.fasta] ID=asmbl_7975\|g.4972 ORF ID=asmbl_7975\|g.4972 ID=asmbl_7975\|m.4972 type:complete len:315 (+) ID=asmbl_7975:219-1163(+) [Y12_quiver_Trinity_v2_2_0_PASA_2June2016-contam.fasta]
Organism:	Saccharomyces cerevisiae
Length:	314 amino acids
Gene Ontology:
Cellular Component:	incipient cellular bud site [IDA] cellular bud tip [IDA] cellular bud neck [IDA] mating projection tip [IDA] actin cap [IDA]
Biological Process:	axial cellular bud site selection [IMP] regulation of exit from mitosis [IMP][IPI] Rho protein signal transduction [IPI]
Molecular Function:	small GTPase regulator activity [TAS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MTEGKRLQQM ELPQMKSIWI DEDQEMEKLY GFQVRQRFMN GPSTDSDEDA DEDLGIVLVD  60
   61 SKKLALPNKN NIKLPPLPNY MTINPNINSN HKSLTNKKKN FLGMFKKKDL LSRRHGSAKT 120
  121 AKQSSISTPF DFHHISHANG KREDNPLESH EEKHDVESLV KFTSLAPQPR PDSNVSSKYS 180
  181 NVVMNDSSRI VSSSTIATTM DSHHDGNETN NTPNGNKQLD SPTDLEMTLE DLRNYTFPSV 240
  241 LGDSVSEKTN PSSPSVSSFS GKFKPRELSA LHTPELGNCF NVDQSLNSPG NRISVDDVLK 300
  301 FYYQCSETST PRNT

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Krogan NJ, et al. (2006)		FIG2 GIC1 MRP13 YIL067C
View Details	Qiu et al. (2008)		BFA1 BUD5 BUD6 CDC24 GIC1 MOB2 PEA2 PFY1 SLG1

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	MLP2	#10 Mitotic Prep1-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	GIC1	GIC2	2	Drees BL, et al. (2001)
View Screen	GIC1	GIC1	2	Drees BL, et al. (2001)
View Screen	CDC42	GIC1	2	Drees BL, et al. (2001)
View Screen	GIC1	CDC12	2	Drees BL, et al. (2001)
View Screen	BUB2	GIC1	2	Uetz P, et al. (2000)
View Screen	GIC1	RRP14	2	Drees BL, et al. (2001)
View Screen	GIC1	ZDS2	2	Drees BL, et al. (2001)
View Screen	GIC1	BUB2	2	Drees BL, et al. (2001)
View Screen	GIC1	DFG5	2	Drees BL, et al. (2001)
View Screen	GIC1	CLA4	2	Drees BL, et al. (2001)
View Screen	GIC1	STE50	2	Drees BL, et al. (2001)
View Screen	GIC1	CSM1	2	Drees BL, et al. (2001)
View Screen	GIC1	BEM4	2	Drees BL, et al. (2001)

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	GIC1	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..99]	deduced	N/A	Confident ab initio structure predictions are available.
2	View Details	[100..241]	Pfam	17.376751	P21-Rho-binding domain No confident structure predictions are available.
3	View Details	[242..314]	deduced	N/A	No confident structure predictions are available.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2