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Protein Overview: ECM32

General Information:
Name(s) found: ECM32 / YER176W [SGD]
Description(s) found:
Found 22 descriptions.
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  • DNA dependent ATPase/DNA helicase belonging to the Dna2p- and Nam7p-like family of helicases that is involved in modulating translation termination; interacts with the translation termination factors, localized to polysomes [SGD]
    • Organism: Saccharomyces cerevisiae
    Length: 1121 amino acids

    Gene Ontology:
    Cellular Component: polysome [IDALink to PubMed article]
    Biological Process: regulation of translational termination [IMPLink to PubMed article]
    Molecular Function: DNA helicase activity [IDALink to PubMed article]
    RNA helicase activity [ISSLink to PubMed article]


    Sequence:
    Sequence:
    [PDR BLAST]
    [ProtParam] 
          1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MDFQCRTCSQ AYDAEQMMKH LSSTRHKTVF DTSNDEDICC EECQDKNIHQ LQIIRFGGED  60
   61 MVLLCNSCFR KEYSETERPS TSYSLQNGSI LKFWEKYVKV RECCCDECGE ESNLNANRNG 120
  121 EVLCDKCLPK SNRAKDFVSE KSGRFLYIYL GLNETQNSTR KPRKKGGRRV GRGKKGRKGA 180
  181 KIKKEKKETF EAKISRIAYE VKKENSTIQS SSSSNLRNFK GFKAVESDPV VAAKVSKSET 240
  241 SRSNPGPSNR NKGKGNKANH KKNSGNGIGK EKERKTNIRN NVRNSQPIPE DRKNTNSHVT 300
  301 TNSGGKGKNE SVDKHQLPQP KALNGNGSGS TNTTGLKKGK KDHAGQKTKG NDKTGNKNPR 360
  361 EAKLNSAGRK NALGKKSNNQ PNKGTSRWTI GSDTESSREP SISPNENTTS ITKSRNRNKK 420
  421 ASKPTLNEKS KTTTMPKKLE TKNQEKNNGK TKDGKLIYEE GEPLTRYNTF KSTLSYPDLN 480
  481 TYLNDYSFAL FLEQKLENEF VQNFNILWPR NEKDTAFIIN VEKNNNSELE KLLPANLLAL 540
  541 GRPAFNERQP FFFCTQDEQK VWYIFIKELS IQRGKYVLLV ELFSWNNLSL PTKNGSSQFK 600
  601 LLPTSAQTSR ILFAMTRITN PKFIDLLLGQ KPIKEIYFDN RLKFSSDKLN RSQKTAVEHV 660
  661 LNNSITILQG PPGTGKTSTI EEIIIQVIER FHAFPILCVA ASNIAIDNIA EKIMENRPQI 720
  721 KILRILSKKK EQQYSDDHPL GEICLHNIVY KNLSPDMQVV ANKTRRGEMI SKSEDTKFYK 780
  781 EKNRVTNKVV SQSQIIFTTN IAAGGRELKV IKECPVVIMD EATQSSEAST LVPLSLPGIR 840
  841 NFVFVGDEKQ LSSFSNIPQL ETSLFERVLS NGTYKNPLML DTQYRMHPKI SEFPIKKIYN 900
  901 GELKDGVTDE QKAWPGVQHP LFFYQCDLGP ESRVRSTQRD IVGFTYENKH ECVEIVKIIQ 960
  961 ILMLDKKVPL EEIGVITPYS AQRDLLSDIL TKNVVINPKQ ISMQQEYDEI ELFNAAGSQG1020
 1021 TAGSLQNNVI NIINGLHVAT VDSFQGHEKS FIIFSCVRNN TENKIGFLRD KRRLNVALTR1080
 1081 AKHGLIVVGN KNVLRKGDPL WKDYITYLEE QEVIFTDLTA Y

    Protein Complex Data

      PUBLICATION TOPOLOGY COCOMPLEXED PROTEINS
    View Details (MIPS) Mewes HW, et al. (2004) DNA2 ECM32
    View Details Qiu et al. (2008) ECM32 MKT1 NAM7

    Mass Spectrometry Data

    The following runs contain data for this protein:

      BAIT COMMENTS PUBLICATION
    View Run RPA135 No Comments Schneider, DA, et al. (2006)
    View Run BOI1 Sample boi1 with gst from october 2005 McCusker D, et al (2007)
    View Run MLP2 #32 Asynchronous Prep-No Phosphopeptide enrichment Keck JM, et al. (2011)
    View Run MLP2 #12 Mitotic Prep1-TiO2 Flowthrough Keck JM, et al. (2011)
    View Run MLP2 #11 Mitotic Prep1-TiO2 enriched, new search criteria Keck JM, et al. (2011)

    Yeast Two-Hybrid Data

    The following interactions contain this protein:

    NOT SHOWING SINGLE HITS. [ Show Single Hits ]

    [View our yeast two-hybrid interpretation guidelines.]

    No yeast two-hybrid interactions found for this protein.

    Microscopy / Localization Data

    New Feature: Upload Your Own Microscopy Data

      PROTEIN(S) PUBLICATION
    View Data ECM32 Huh WK, et al. (2003)

    Protein Structure Data


    [What does the above image mean?]
    [View Top Sequence Alignments] [Show Ginzu Version Information]

    Domains predicted:

    #   Region(s) Method Confidence Match Description
    1 View Details [1..382] ORFEUS 8.07 RBP1
    2 View Details [383..461] ORFEUS 10.24 No description for 1l8mA was found.
    3 View Details [462..632] deduced N/A No confident structure predictions are available.
    4 View Details [633..1121] PSI-BLAST 80.30103 DEXX box DNA helicase

    Functions predicted (by domain):

    # Gene Ontology predictions
    1 No functions predicted.
    2 No functions predicted.
    3 No functions predicted.
    4
    Term Confidence Notes
  • structural constituent of ribosome
    		•  3.070470376968 bayes_pls_golite062009
  • structural molecule activity
    		•  2.08211451183607 bayes_pls_golite062009
  • binding
    		•  2.00548409930693 bayes_pls_golite062009
  • translation release factor activity
    		•  1.47279606301067 bayes_pls_golite062009
  • translation termination factor activity
    		•  1.47162484856171 bayes_pls_golite062009
  • nucleic acid binding
    		•  1.24151268097078 bayes_pls_golite062009
  • catalytic activity
    		•  1.00104173493322 bayes_pls_golite062009
  • DNA binding
    		•  0.829652732561224 bayes_pls_golite062009
  • transcription regulator activity
    		•  0.648798858522413 bayes_pls_golite062009
  • purine nucleotide binding
    		•  0.569910969126728 bayes_pls_golite062009
  • purine ribonucleotide binding
    		•  0.568840867400671 bayes_pls_golite062009
  • ribonucleotide binding
    		•  0.568779924702229 bayes_pls_golite062009
  • nucleotide binding
    		•  0.564275755355315 bayes_pls_golite062009
  • translation regulator activity
    		•  0.493216082928152 bayes_pls_golite062009
  • translation factor activity, nucleic acid binding
    		•  0.480200274620277 bayes_pls_golite062009
  • translation elongation factor activity
    		•  0.322643574778 bayes_pls_golite062009
  • RNA helicase activity
    		•  0.2302541318658 bayes_pls_golite062009
  • ATP binding
    		•  0.1860481660648 bayes_pls_golite062009
  • protein binding
    		•  0.170837278876362 bayes_pls_golite062009
  • adenyl ribonucleotide binding
    		•  0.152859582615242 bayes_pls_golite062009
  • adenyl nucleotide binding
    		•  0.138615225841109 bayes_pls_golite062009
  • RNA binding
    		•  0.0693163519626152 bayes_pls_golite062009
  • nucleoside-triphosphatase activity
    		•  0.0530667986761242 bayes_pls_golite062009
  • pyrophosphatase activity
    		•  0.0482442222493492 bayes_pls_golite062009
  • hydrolase activity, acting on acid anhydrides
    		•  0.028470639373934 bayes_pls_golite062009
  • hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides
    		•  0.0265151533232588 bayes_pls_golite062009
  • RNA-dependent ATPase activity
    		•  0.0067235917574795 bayes_pls_golite062009


    Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076


    Philius Transmembrane Prediction:

    Protein predicted to be: GLOBULAR (No transmembrane regions or signal peptide)
    Confidence of classification: 0.99

    Source: Reynolds et al. (2008)

    YRC Informatics Platform - Version 3.0
    Created and Maintained by: Michael Riffle