Protein Overview: AFG1

General Information:
Name(s) found:	AFG1 / YEL052W [SGD]
Description(s) found: Found 23 descriptions. SHOW ALL	Conserved protein that may act as a chaperone in the degradation of misfolded or unassembled cytochrome c oxidase subunits; localized to matrix face of the mitochondrial inner membrane; member of the AAA family but lacks a protease domain [SGD]
Organism:	Saccharomyces cerevisiae
Length:	509 amino acids
Gene Ontology:
Cellular Component:	mitochondrion [IDA ]
Biological Process:	biological_process [ND]
Molecular Function:	ATPase activity [ISS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MIALKPNAVR TFRQVQHCSF RICRYQSTKS NKCLTPLQEY DRLVKLGKLR DDTYQRGIIS  60
   61 SLGDLYDSLV KYVPPVVKTP NAVDQVGGWL NGLKSVFSRG KPKNIGAYVD VSKIGNSIPR 120
  121 GVYLYGDVGC GKTMLMDLFY TTIPNHLTKK RIHFHQFMQY VHKRSHEIVR EQNLKELGDA 180
  181 KGKEIDTVPF LAAEIANNSH VLCFDEFQVT DVADAMILRR LMTALLSDDY GVVLFATSNR 240
  241 HPDELYINGV QRQSFIPCIE LIKHRTKVIF LNSPTDYRKI PRPVSSVYYF PSDTSIKYAS 300
  301 KECKTRRETH IKEWYNYFAQ ASHTDDSTDS HTVHKTFYDY PLTIWGREFK VPKCTPPRVA 360
  361 QFTFKQLCGE PLAAGDYLTL AKNFEAFIVT DIPYLSIYVR DEVRRFITFL DAVYDSGGKL 420
  421 ATTGAADFSS LFVEPEQILN DFELRPTTKE PDSVDTGMVD EMVEKHGFSK EIAKKSQMFA 480
  481 LDEERFAFAR ALSRLSQMSS TDWVTKPTY

Protein Complex Data

No complex found for this protein.

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)
View Run	MLP2	#29 Asynchronous Prep5-TiO2 Flowthrough	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	ELM1	AFG1	1	Unpublished Fields Lab Data
View Screen	YIF1	AFG1	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	AFG1	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..252]	PSI-BLAST	56.30103	Membrane fusion atpase p97 N-terminal domain , P97-Nn; Membrane fusion atpase p97, D1 domain; Membrane fusion atpase p97 domain 2, P97-Nc
2	View Details	[253..356]	PSI-BLAST	56.30103	Membrane fusion atpase p97 N-terminal domain , P97-Nn; Membrane fusion atpase p97, D1 domain; Membrane fusion atpase p97 domain 2, P97-Nc
3	View Details	[357..509]	MSA	1.097982	View MSA. No confident structure predictions are available.

Functions predicted (by domain):

Gene Ontology predictions

No functions predicted.

Term	Confidence	Notes
transporter activity	3.10463051760206	bayes_pls_golite062009
transmembrane transporter activity	2.95886887664984	bayes_pls_golite062009
nucleoside-triphosphatase activity	2.74389108884083	bayes_pls_golite062009
pyrophosphatase activity	2.67097555143215	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides	2.65161658635465	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides	2.64849912301318	bayes_pls_golite062009
active transmembrane transporter activity	2.50494515048649	bayes_pls_golite062009
primary active transmembrane transporter activity	2.39629638016861	bayes_pls_golite062009
P-P-bond-hydrolysis-driven transmembrane transporter activity	2.39600931083427	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides, catalyzing transmembrane movement of substances	2.36051733655256	bayes_pls_golite062009
ATPase activity, coupled to movement of substances	2.35906629925754	bayes_pls_golite062009
ATPase activity, coupled to transmembrane movement of substances	2.35679406876731	bayes_pls_golite062009
hydrolase activity	2.32318595170495	bayes_pls_golite062009
ATPase activity	2.2625504298178	bayes_pls_golite062009
ATPase activity, coupled	2.24901251684901	bayes_pls_golite062009
binding	2.0681607836408	bayes_pls_golite062009
substrate-specific transporter activity	1.65609907941094	bayes_pls_golite062009
protein transporter activity	1.37245678118001	bayes_pls_golite062009
substrate-specific transmembrane transporter activity	1.3182108176243	bayes_pls_golite062009
catalytic activity	1.00104173493322	bayes_pls_golite062009
purine nucleotide binding	0.929901285538132	bayes_pls_golite062009
nucleotide binding	0.922442819194247	bayes_pls_golite062009
purine ribonucleotide binding	0.920987930180051	bayes_pls_golite062009
ribonucleotide binding	0.920926158369519	bayes_pls_golite062009
motor activity	0.70874549510465	bayes_pls_golite062009
ion transmembrane transporter activity	0.704934361725919	bayes_pls_golite062009
guanyl nucleotide binding	0.640772201693186	bayes_pls_golite062009
guanyl ribonucleotide binding	0.63468044350616	bayes_pls_golite062009
GTP binding	0.621689261700018	bayes_pls_golite062009
protein transmembrane transporter activity	0.46552249844591	bayes_pls_golite062009
GTPase activity	0.437665353870877	bayes_pls_golite062009
ATP binding	0.41092997612902	bayes_pls_golite062009
adenyl ribonucleotide binding	0.385813421701492	bayes_pls_golite062009
adenyl nucleotide binding	0.377870145186619	bayes_pls_golite062009
nucleic acid binding	0.318452816722389	bayes_pls_golite062009
cation transmembrane transporter activity	0.282397764628576	bayes_pls_golite062009
protein binding	0.193768631623192	bayes_pls_golite062009
P-P-bond-hydrolysis-driven protein transmembrane transporter activity	0.00571427458217011	bayes_pls_golite062009
macromolecule transmembrane transporter activity	0.00571427458217011	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2