Protein Overview: HXT3

General Information:
Name(s) found:	HXT3 / YDR345C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6320552\|ref\|NP_010632.1\| Low affinity glucose transporter of the major facilitator superfamily, expression is induced in low or high glucose conditions; Hxt3p [Saccharomyces cerevisiae] [NCBI NR] gi\|417164\|sp\|P32466.1\|HXT3_YEAST RecName: Full=Low-affinity glucose transporter HXT3 [NCBI NR] pir\|\|S31294 hexose transport protein HXT3 - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|171672\|gb\|AAA34672.1\| hexose transporter [Saccharomyces cerevisiae] [NCBI NR] gi\|1230672\|gb\|AAB64781.1\| Hxt3p: High-affinity glucose transporter (Swiss Prot. accession number P32466) [Saccharomyces cerevisiae] [NCBI NR] Low-affinity glucose transporter HXT3 OS=Saccharomyces cerevisiae GN=HXT3 PE=1 SV=1 [Swiss-Prot] HXT3 Low-affinity hexose facilitator [mips] Low affinity glucose transporter of the major facilitator superfamily, expression is induced in low or high glucose conditions [SGD] HXT3, Chr IV from 1162947-1164650, reverse complement [yeast_orfs2] Chr IV from 1162947-1164650, reverse complement [yeastorf3.fasta] Chr IV from 1162946-1164649, reverse complement [yeastorf4.fasta] Chr IV from 1164649-1162946, reverse complement [yeastorf5.fasta] (P32466) Low-affinity glucose transporter HXT3 [4932.FASTACCERV.txt] HXT3 SGDID:S0002753, Chr IV from 1164650-1162947, reverse complement, Verified ORF [yeastflysequest.fasta] HXT3 SGDID:S0002753, Chr IV from 1164652-1162949, reverse complement, Verified ORF [10112005cervSGD.fasta] Hxt3p [Saccharomyces cerevisiae]gi\|1230672\|gb\|AAB64781.1\| Hxt3p: High-affinity glucose transporter (Swiss Prot. accession number P32466) [Saccharomyces cerevisiae]gi\|320688\|pir\|\|S31294 hexose transport protein HXT3 - yeast (Saccharomyces cerevisiae)gi\| [nrdb_11152004_Chlamydomonas] HXT3 SGDID:S000002753, Chr IV from 1164652-1162949, reverse complement, Verified ORF, "Low affinity glucose transporter of the major facilitator superfamily, expression is induced in low or high glucose conditions" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] HXT3 SGDID:S000002753, Chr IV from 1164654-1162951, reverse complement, Verified ORF, "Low affinity glucose transporter of the major facilitator superfamily, expression is induced in low or high glucose conditions" [yeast-contam.fasta] HXT3 SGDID:S000002753, Chr IV from 1164655-1162952, reverse complement, Verified ORF, "Low affinity glucose transporter of the major facilitator superfamily, expression is induced in low or high glucose conditions" [SGD_S-cerevisiae_na_08-19-2008_con_reversed.fasta] hexose transporter [nr-271106-contam.fasta] Hxt3p: High-affinity glucose transporter (Swiss Prot. accession number P32466) [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Low affinity glucose transporter of the major facilitator superfamily, expression is induced in low or high glucose conditions; Hxt3p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Low-affinity glucose transporter HXT3 [nr-271106-contam.fasta] [chemostatdb.fasta] Low-affinity glucose transporter HXT3 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=HXT3 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] HXT3 SGDID:S000002753, Chr IV from 1164660-1162957, Genome Release 64-1-1, reverse complement, Verified ORF, "Low affinity glucose transporter of the major facilitator superfamily, expression is induced in low or high glucose conditions" [yeast-030211-contam.fasta] HXT3 SGDID:S000002753, Chr IV from 1164660-1162957, Genome Release 64-2-1, reverse complement, Verified ORF, "Low affinity glucose transporter of the major facilitator superfamily; expression is induced in low or high glucose conditions; HXT3 has a paralog, HXT5, that arose from the whole genome duplication" [20141208_orf_trans.fasta] YDR345C SGDID:S000002753, Chr IV from 1164660-1162957, Genome Release 64-1-1, reverse complement, Verified ORF, "Low affinity glucose transporter of the major facilitator superfamily, expression is induced in low or high glucose conditions" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	567 amino acids
Gene Ontology:
Cellular Component:	plasma membrane [ISS]
Biological Process:	hexose transport [TAS]
Molecular Function:	fructose transmembrane transporter activity [TAS] glucose transmembrane transporter activity [IDA][TAS] mannose transmembrane transporter activity [TAS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MNSTPDLISP QKSSENSNAD LPSNSSQVMN MPEEKGVQDD FQAEADQVLT NPNTGKGAYV  60
   61 TVSICCVMVA FGGFVFGWDT GTISGFVAQT DFLRRFGMKH KDGSYYLSKV RTGLIVSIFN 120
  121 IGCAIGGIIL AKLGDMYGRK MGLIVVVVIY IIGIIIQIAS INKWYQYFIG RIISGLGVGG 180
  181 IAVLSPMLIS EVAPKEMRGT LVSCYQLMIT LGIFLGYCTN FGTKNYSNSV QWRVPLGLCF 240
  241 AWALFMIGGM TFVPESPRYL VEAGQIDEAR ASLSKVNKVA PDHPFIQQEL EVIEASVEEA 300
  301 RAAGSASWGE LFTGKPAMFK RTMMGIMIQS LQQLTGDNYF FYYGTTVFNA VGMSDSFETS 360
  361 IVFGVVNFFS TCCSLYTVDR FGRRNCLLYG AIGMVCCYVV YASVGVTRLW PNGEGNGSSK 420
  421 GAGNCMIVFA CFYIFCFATT WAPIAYVVIS ETFPLRVKSK AMSIATAANW LWGFLIGFFT 480
  481 PFITGAINFY YGYVFMGCMV FAYFYVFFFV PETKGLTLEE VNDMYAEGVL PWKSASWVPT 540
  541 SQRGANYDAD ALMHDDQPFY KKMFGKK

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Qiu et al. (2008)		HXT1 HXT3 HXT4 HXT8

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	MIA40	No Comments	Hazbun TR, et al. (2003)
View Run	DAD1	Dam1-765 complex purified from yeast with Dad1-TAP	Shimogawa MM, et al. (2006)
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)
View Run	VAM3	Sample: HX12 - trans-SNARE complex formation and fusion are inhibited (control).	Hao Xu, et al. (2010)
View Run	VAM3	Sample: HX13 - trans-SNARE complex forms but fusion is blocked.	Hao Xu, et al. (2010)
View Run	VAM3	Sample: HX14 - mixture in detergent (control).	Hao Xu, et al. (2010)
View Run	MLP2	#32 Asynchronous Prep-No Phosphopeptide enrichment	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	HMT1	HXT3	1	Unpublished Fields Lab Data
View Screen	TRL1	HXT3	1	Unpublished Fields Lab Data
View Screen	SPT21	HXT3	1	Unpublished Fields Lab Data

Microscopy / Localization Data

New Feature: Upload Your Own Microscopy Data

	PROTEIN(S)	PUBLICATION
View Data	HXT3	Huh WK, et al. (2003)

Protein Structure Data

[What does the above image mean?]
[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..254] [303..514]	PSI-BLAST	472.218487	No description for 1ja5A_ was found.
2	View Details	[255..302] [515..567]	PSI-BLAST	472.218487	No description for 1ja5A_ was found.

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
glycerol transmembrane transporter activity	7.11654923455995	bayes_pls_golite062009
alcohol transmembrane transporter activity	6.7545566917184	bayes_pls_golite062009
polyol transmembrane transporter activity	6.7545566917184	bayes_pls_golite062009
glycerol-3-phosphate transmembrane transporter activity	5.74077413704789	bayes_pls_golite062009
transporter activity	4.09176719149278	bayes_pls_golite062009
substrate-specific transporter activity	3.59744164024479	bayes_pls_golite062009
transmembrane transporter activity	3.5865018981083	bayes_pls_golite062009
substrate-specific transmembrane transporter activity	3.54194478414824	bayes_pls_golite062009
hexose phosphate transmembrane transporter activity	3.3746965264994	bayes_pls_golite062009
glucose-6-phosphate transmembrane transporter activity	3.3746965264994	bayes_pls_golite062009
sugar:hydrogen symporter activity	2.99050613058546	bayes_pls_golite062009
cation:sugar symporter activity	2.99050613058546	bayes_pls_golite062009
monosaccharide transmembrane transporter activity	2.63293544825311	bayes_pls_golite062009
carbohydrate transmembrane transporter activity	2.62141276633776	bayes_pls_golite062009
sugar transmembrane transporter activity	2.47557586092261	bayes_pls_golite062009
ion transmembrane transporter activity	2.21266150105753	bayes_pls_golite062009
solute:hydrogen symporter activity	2.10485633822652	bayes_pls_golite062009
binding	1.89539096965643	bayes_pls_golite062009
cation transmembrane transporter activity	1.79369588808394	bayes_pls_golite062009
active transmembrane transporter activity	1.59419818573076	bayes_pls_golite062009
secondary active transmembrane transporter activity	1.22264637452409	bayes_pls_golite062009
symporter activity	0.74591237387473	bayes_pls_golite062009
solute:cation symporter activity	0.74591237387473	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

[View Details]

Source: Reynolds et al. 2008. Manuscript submitted

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2