Protein Overview: GEM1

General Information:
Name(s) found:	GEM1 / YAL048C [SGD]
Description(s) found: Found 25 descriptions. SHOW ALL	Evolutionarily-conserved tail-anchored outer mitochondrial membrane GTPase which regulates mitochondrial morphology; cells lacking Gem1p contain collapsed, globular, or grape-like mitochondria; not required for pheromone-induced cell death [SGD]
Organism:	Saccharomyces cerevisiae
Length:	662 amino acids
Gene Ontology:
Cellular Component:	mitochondrion [IDA] mitochondrial outer membrane [IDA ]
Biological Process:	vesicle-mediated transport [IGI][IMP]
Molecular Function:	GTPase activity [IDA]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MTKETIRVVI CGDEGVGKSS LIVSLTKAEF IPTIQDVLPP ISIPRDFSSS PTYSPKNTVL  60
   61 IDTSDSDLIA LDHELKSADV IWLVYCDHES YDHVSLFWLP HFRSLGLNIP VILCKNKCDS 120
  121 ISNVNANAMV VSENSDDDID TKVEDEEFIP ILMEFKEIDT CIKTSAKTQF DLNQAFYLCQ 180
  181 RAITHPISPL FDAMVGELKP LAVMALKRIF LLSDLNQDSY LDDNEILGLQ KKCFNKSIDV 240
  241 NELNFIKDLL LDISKHDQEY INRKLYVPGK GITKDGFLVL NKIYAERGRH ETTWAILRTF 300
  301 HYTDSLCIND KILHPRLVVP DTSSVELSPK GYRFLVDIFL KFDIDNDGGL NNQELHRLFK 360
  361 CTPGLPKLWT STNFPFSTVV NNKGCITLQG WLAQWSMTTF LNYSTTTAYL VYFGFQEDAR 420
  421 LALQVTKPRK MRRRSGKLYR SNINDRKVFN CFVIGKPCCG KSSLLEAFLG RSFSEEYSPT 480
  481 IKPRIAVNSL ELKGGKQYYL ILQELGEQEY AILENKDKLK ECDVICLTYD SSDPESFSYL 540
  541 VSLLDKFTHL QDLPLVFVAS KADLDKQQQR CQIQPDELAD ELFVNHPLHI SSRWLSSLNE 600
  601 LFIKITEAAL DPGKNTPGLP EETAAKDVDY RQTALIFGST VGFVALCSFT LMKLFKSSKF 660
  661 SK

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Gavin AC, et al. (2006)		GEM1 HUA2

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	ASF1	3829 - low filter	Green EM, et al (2005)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	HMT1	GEM1	1	Unpublished Fields Lab Data
View Screen	PCF11	GEM1	1	Unpublished Fields Lab Data
View Screen	ATG7	GEM1	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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No microscopy data found in the PDR for this protein.

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..197]	PSI-BLAST	106.522879	Rac
2	View Details	[198..289]	ORFEUS	10.03	Troponin C
3	View Details	[290..430]	ORFEUS	10.03	Troponin C
4	View Details	[431..662]	PSI-BLAST	82.9897	CDC42

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
microtubule motor activity	3.64858540496165	bayes_pls_golite062009
transporter activity	3.10463051760206	bayes_pls_golite062009
transmembrane transporter activity	2.95886887664984	bayes_pls_golite062009
nucleoside-triphosphatase activity	2.89122312915795	bayes_pls_golite062009
pyrophosphatase activity	2.81593838944939	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides	2.79724985811732	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides	2.79347973548337	bayes_pls_golite062009
binding	2.64537357529343	bayes_pls_golite062009
primary active transmembrane transporter activity	2.61775212528604	bayes_pls_golite062009
P-P-bond-hydrolysis-driven transmembrane transporter activity	2.61467977505707	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides, catalyzing transmembrane movement of substances	2.57552435627908	bayes_pls_golite062009
ATPase activity, coupled to transmembrane movement of substances	2.57107347424517	bayes_pls_golite062009
ATPase activity, coupled to movement of substances	2.57054427875021	bayes_pls_golite062009
active transmembrane transporter activity	2.50494515048649	bayes_pls_golite062009
ATPase activity	2.44176688200537	bayes_pls_golite062009
ATPase activity, coupled	2.43862163407966	bayes_pls_golite062009
microfilament motor activity	2.38215814751368	bayes_pls_golite062009
motor activity	2.1532118704195	bayes_pls_golite062009
substrate-specific transporter activity	1.91091452720653	bayes_pls_golite062009
guanyl nucleotide binding	1.89320355239381	bayes_pls_golite062009
guanyl ribonucleotide binding	1.88721318481223	bayes_pls_golite062009
GTP binding	1.87900088157196	bayes_pls_golite062009
substrate-specific transmembrane transporter activity	1.56325751172863	bayes_pls_golite062009
hydrolase activity	1.40217504818333	bayes_pls_golite062009
GTPase activity	1.23010905798386	bayes_pls_golite062009
molecular transducer activity	1.13474466330485	bayes_pls_golite062009
signal transducer activity	1.13474466330485	bayes_pls_golite062009
protein transporter activity	1.05248757342537	bayes_pls_golite062009
actin-dependent ATPase activity	0.990320704369021	bayes_pls_golite062009
ion transmembrane transporter activity	0.977237423302079	bayes_pls_golite062009
purine nucleotide binding	0.929901285538132	bayes_pls_golite062009
nucleotide binding	0.922442819194247	bayes_pls_golite062009
purine ribonucleotide binding	0.920987930180051	bayes_pls_golite062009
ribonucleotide binding	0.920926158369519	bayes_pls_golite062009
nucleic acid binding	0.777787753097919	bayes_pls_golite062009
cytoskeletal protein binding	0.568484017802328	bayes_pls_golite062009
cation transmembrane transporter activity	0.557919489256916	bayes_pls_golite062009
DNA binding	0.479412476059825	bayes_pls_golite062009
ATP binding	0.41092997612902	bayes_pls_golite062009
adenyl ribonucleotide binding	0.385813421701492	bayes_pls_golite062009
receptor activity	0.381142123108547	bayes_pls_golite062009
adenyl nucleotide binding	0.377870145186619	bayes_pls_golite062009
transmembrane receptor activity	0.355770151889237	bayes_pls_golite062009
tubulin binding	0.326239364039846	bayes_pls_golite062009
transcription regulator activity	0.312171320541728	bayes_pls_golite062009
macromolecule transmembrane transporter activity	0.28297830193871	bayes_pls_golite062009
P-P-bond-hydrolysis-driven protein transmembrane transporter activity	0.28297830193871	bayes_pls_golite062009
plus-end-directed microtubule motor activity	0.226790072388501	bayes_pls_golite062009
microtubule binding	0.225323885896927	bayes_pls_golite062009
hydrogen ion transporting ATP synthase activity, rotational mechanism	0.213018989352421	bayes_pls_golite062009
protein binding	0.170837278876362	bayes_pls_golite062009
inorganic cation transmembrane transporter activity	0.123304946374679	bayes_pls_golite062009
metal ion transmembrane transporter activity	0.0775601177068852	bayes_pls_golite062009
actin binding	0.0206144204131673	bayes_pls_golite062009

Term	Confidence	Notes
binding	3.05873877511752	bayes_pls_golite062009
microfilament motor activity	1.9984373493184	bayes_pls_golite062009
metal ion binding	1.78612329591962	bayes_pls_golite062009
cation binding	1.78612329591962	bayes_pls_golite062009
ion binding	1.7794276789734	bayes_pls_golite062009
molecular transducer activity	1.57685201816793	bayes_pls_golite062009
signal transducer activity	1.57685201816793	bayes_pls_golite062009
cytoskeletal protein binding	1.52831937678894	bayes_pls_golite062009
actin-dependent ATPase activity	1.26291497318874	bayes_pls_golite062009
motor activity	1.22884221769274	bayes_pls_golite062009
transporter activity	1.18173318787461	bayes_pls_golite062009
protein binding	1.09433047543741	bayes_pls_golite062009
transmembrane transporter activity	0.967232199555998	bayes_pls_golite062009
calcium ion binding	0.96333115642547	bayes_pls_golite062009
enzyme regulator activity	0.898362179169385	bayes_pls_golite062009
substrate-specific transporter activity	0.713088884599039	bayes_pls_golite062009
actin binding	0.571065725792783	bayes_pls_golite062009
substrate-specific transmembrane transporter activity	0.471661454726807	bayes_pls_golite062009
transcription regulator activity	0.467743512816825	bayes_pls_golite062009
active transmembrane transporter activity	0.255162242356474	bayes_pls_golite062009
enzyme activator activity	0.224800334961602	bayes_pls_golite062009
ion transmembrane transporter activity	0.206190076128058	bayes_pls_golite062009
nucleic acid binding	0.137921428032586	bayes_pls_golite062009
DNA binding	0.126696463568471	bayes_pls_golite062009
tubulin binding	0.121694931665203	bayes_pls_golite062009
gated channel activity	0.0726874243760007	bayes_pls_golite062009
cation transmembrane transporter activity	0.0385263849284123	bayes_pls_golite062009

Term	Confidence	Notes
microtubule motor activity	3.64858540496165	bayes_pls_golite062009
transporter activity	3.10463051760206	bayes_pls_golite062009
transmembrane transporter activity	2.95886887664984	bayes_pls_golite062009
nucleoside-triphosphatase activity	2.89122312915795	bayes_pls_golite062009
pyrophosphatase activity	2.81593838944939	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides	2.79724985811732	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides	2.79347973548337	bayes_pls_golite062009
binding	2.64537357529343	bayes_pls_golite062009
primary active transmembrane transporter activity	2.61775212528604	bayes_pls_golite062009
P-P-bond-hydrolysis-driven transmembrane transporter activity	2.61467977505707	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides, catalyzing transmembrane movement of substances	2.57552435627908	bayes_pls_golite062009
ATPase activity, coupled to transmembrane movement of substances	2.57107347424517	bayes_pls_golite062009
ATPase activity, coupled to movement of substances	2.57054427875021	bayes_pls_golite062009
active transmembrane transporter activity	2.50494515048649	bayes_pls_golite062009
ATPase activity	2.44176688200537	bayes_pls_golite062009
ATPase activity, coupled	2.43862163407966	bayes_pls_golite062009
microfilament motor activity	2.38215814751368	bayes_pls_golite062009
motor activity	2.1532118704195	bayes_pls_golite062009
substrate-specific transporter activity	1.91091452720653	bayes_pls_golite062009
guanyl nucleotide binding	1.89320355239381	bayes_pls_golite062009
guanyl ribonucleotide binding	1.88721318481223	bayes_pls_golite062009
GTP binding	1.87900088157196	bayes_pls_golite062009
substrate-specific transmembrane transporter activity	1.56325751172863	bayes_pls_golite062009
hydrolase activity	1.40217504818333	bayes_pls_golite062009
GTPase activity	1.23010905798386	bayes_pls_golite062009
molecular transducer activity	1.13474466330485	bayes_pls_golite062009
signal transducer activity	1.13474466330485	bayes_pls_golite062009
protein transporter activity	1.05248757342537	bayes_pls_golite062009
actin-dependent ATPase activity	0.990320704369021	bayes_pls_golite062009
ion transmembrane transporter activity	0.977237423302079	bayes_pls_golite062009
purine nucleotide binding	0.929901285538132	bayes_pls_golite062009
nucleotide binding	0.922442819194247	bayes_pls_golite062009
purine ribonucleotide binding	0.920987930180051	bayes_pls_golite062009
ribonucleotide binding	0.920926158369519	bayes_pls_golite062009
nucleic acid binding	0.777787753097919	bayes_pls_golite062009
cytoskeletal protein binding	0.568484017802328	bayes_pls_golite062009
cation transmembrane transporter activity	0.557919489256916	bayes_pls_golite062009
DNA binding	0.479412476059825	bayes_pls_golite062009
ATP binding	0.41092997612902	bayes_pls_golite062009
adenyl ribonucleotide binding	0.385813421701492	bayes_pls_golite062009
receptor activity	0.381142123108547	bayes_pls_golite062009
adenyl nucleotide binding	0.377870145186619	bayes_pls_golite062009
transmembrane receptor activity	0.355770151889237	bayes_pls_golite062009
tubulin binding	0.326239364039846	bayes_pls_golite062009
transcription regulator activity	0.312171320541728	bayes_pls_golite062009
macromolecule transmembrane transporter activity	0.28297830193871	bayes_pls_golite062009
P-P-bond-hydrolysis-driven protein transmembrane transporter activity	0.28297830193871	bayes_pls_golite062009
plus-end-directed microtubule motor activity	0.226790072388501	bayes_pls_golite062009
microtubule binding	0.225323885896927	bayes_pls_golite062009
hydrogen ion transporting ATP synthase activity, rotational mechanism	0.213018989352421	bayes_pls_golite062009
protein binding	0.170837278876362	bayes_pls_golite062009
inorganic cation transmembrane transporter activity	0.123304946374679	bayes_pls_golite062009
metal ion transmembrane transporter activity	0.0775601177068852	bayes_pls_golite062009
actin binding	0.0206144204131673	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

[View Details]

Source: Reynolds et al. 2008. Manuscript submitted

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2