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Protein Overview: MAK16

General Information:
Name(s) found: MAK16 / YAL025C [SGD]
Description(s) found:
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  • pir||BVBYK6 MAK16 protein - yeast (Saccharomyces cerevisiae) [NCBI NR]
  • gi|6319294|ref|NP_009377.1| Essential nuclear protein, constituent of 66S pre-ribosomal particles; required for maturation of 25S and 5.8S rRNAs; required for maintenance of M1 satellite double-stranded RNA of the L-A virus; Mak16p [Saccharomyces cerevisi [NCBI NR]
  • gi|595561|gb|AAC05007.1| Mak16p: putative nuclear protein [Saccharomyces cerevisiae] [NCBI NR]
  • gi|171880|gb|AAA34752.1| MAK16 protein [NCBI NR]
  • gi|127112|sp|P10962.1|MAK16_YEAST RecName: Full=Protein MAK16; AltName: Full=Maintenance of killer protein 16 [NCBI NR]
  • (P10962) MAK16 protein [Swiss-Prot]
  • Protein MAK16 OS=Saccharomyces cerevisiae GN=MAK16 PE=1 SV=1 [Swiss-Prot]
  • MAK16 Nuclear viral propagation protein [mips]
  • Essential nuclear protein, constituent of 66S pre-ribosomal particles; required for maturation of 25S and 5.8S rRNAs; required for maintenance of M1 satellite double-stranded RNA of the L-A virus [SGD]
  • MAK16, Chr I from 100227-101147, reverse complement [yeast_orfs2]
  • Chr I from 100227-101147, reverse complement [yeastorf3.fasta]
  • Chr I from 101147-100227, reverse complement [yeastorf5.fasta]
  • MAK16 SGDID:S0000023, Chr I from 101148-100228, reverse complement, Verified ORF [yeastflysequest.fasta]
  • MAK16 SGDID:S0000023, Chr I from 101146-100226, reverse complement, Verified ORF [10112005cervSGD.fasta]
  • Essential nuclear protein required for normal concentration of free 60S ribosomal subunits; required for maintenance of M1 satellite double-stranded RNA of the L-A virus [Saccharomyces cerevisiae]gi|127112|sp|P10962|MK16_YEAST MAK16 proteingi|73269|pir| [nrdb_11152004_Chlamydomonas]
  • MAK16 SGDID:S000000023, Chr I from 101146-100226, reverse complement, Verified ORF, "Essential nuclear protein, constituent of 66S pre-ribosomal particles; required for normal concentration of free 60S ribosomal subunits; required for maintenance of M1 sa [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta]
  • MAK16 SGDID:S000000023, Chr I from 101146-100226, reverse complement, Verified ORF, "Essential nuclear protein, constituent of 66S pre-ribosomal particles; required for maturation of 25S and 5.8S rRNAs; required for maintenance of M1 satellite double-stra [SGD_S-cerevisiae_na_08-19-2008_con_reversed.fasta]
  • Protein MAK16 (Maintenance of killer protein 16) [nr-271106-contam.fasta]
  • MAK16 protein [nr-271106-contam.fasta]
  • Essential nuclear protein, constituent of 66S pre-ribosomal particles; required for normal concentration of free 60S ribosomal subunits; required for maintenance of M1 satellite double-stranded RNA of the L-A virus; Mak16p [Saccharomyces cerevisiae] [nr-271106-contam.fasta]
  • Mak16p: putative nuclear protein [Saccharomyces cerevisiae] [nr-271106-contam.fasta]
  • MAK16 SGDID:S000000023, Chr I from 101146-100226, reverse complement, Verified ORF, "Essential nuclear protein, constituent of 66S pre-ribosomal particles; required for maturation of 25S and 5.8S rRNAs; required for maintenance of M1 satellite double-stranded RNA of the L-A virus" [yeast-bovrabprots.fasta]
  • MAK16 SGDID:S000000023, Chr I from 101146-100226, reverse complement, Verified ORF, "Essential nuclear protein, constituent of 66S pre-ribosomal particles; required for normal concentration of free 60S ribosomal subunits; required for maintenance of M1 satellite double-stranded RNA of the L-A virus" [yeast-contam_custom.fasta]
  • [chemostatdb.fasta]
    • Organism: Saccharomyces cerevisiae
    Length: 306 amino acids

    Gene Ontology:
    Cellular Component: nucleolus [IDALink to PubMed article]
    Biological Process: ribosomal large subunit biogenesis [IMPLink to PubMed article]
    ribosome biogenesis [RCALink to PubMed article]
    processing of 27S pre-rRNA [IMPLink to PubMed article]
    Molecular Function: molecular_function [ND]


    Sequence:
    Sequence:
    [PDR BLAST]
    [ProtParam] 
          1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSDEIVWQVI NQSFCSHRIK APNGQNFCRN EYNVTGLCTR QSCPLANSKY ATVKCDNGKL  60
   61 YLYMKTPERA HTPAKLWERI KLSKNYTKAL QQIDEHLLHW SKFFRHKCKQ RFTKLTQVMI 120
  121 TERRLALREE ERHYVGVAPK VKRREQNRER KALVAAKIEK AIEKELMDRL KSGAYGDKPL 180
  181 NVDEKVWKKI MGQMEEENSQ DEEEDWDEEE ESDDGEVEYV ADDGEGEYVD VDDLEKWLAD 240
  241 SDREASSASQ SESDSESESD SDSDEENKNS AKRRKKGTSA KTKRPKVEIE YEEEHEVQNA 300
  301 EQEVAQ

    Protein Complex Data

      PUBLICATION TOPOLOGY COCOMPLEXED PROTEINS
    View Details Ho Y, et al. (2002) ACO1 CIC1 DNL4 GDE1 HTA1 KGD1 MAK16 NOP15 NOP2 NOP7 NSA1 RPF2 RRT6 TIF6 YTM1

    Mass Spectrometry Data

    The following runs contain data for this protein:

      BAIT COMMENTS PUBLICATION
    View Run MLP2 #31 Asynchronous Prep (Protease cleavage) Keck JM, et al. (2011)
    View Run MLP2 #04 Alpha Factor Prep2 Keck JM, et al. (2011)

    Yeast Two-Hybrid Data

    The following interactions contain this protein:

    SHOWING SINGLE HITS. [ Hide Single Hits ]

    [View our yeast two-hybrid interpretation guidelines.]

    No yeast two-hybrid interactions found for this protein.

    Microscopy / Localization Data

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    No microscopy data found in the PDR for this protein.

    Protein Structure Data


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    [View Top Sequence Alignments] [Show Ginzu Version Information]

    Domains predicted:

    #   Region(s) Method Confidence Match Description
    1 View Details [1..247] MSA 6.014942 View MSA. No confident structure predictions are available.
    2 View Details [248..306] deduced N/A No confident structure predictions are available.


    Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076


    Philius Transmembrane Prediction:

    Protein predicted to be: GLOBULAR (No transmembrane regions or signal peptide)
    Confidence of classification: 0.99

    Source: Reynolds et al. (2008)

    YRC Informatics Platform - Version 3.0
    Created and Maintained by: Michael Riffle