YRC Logo
PROTEIN SEARCH:
Descriptions Names[Advanced Search]

Protein Overview: RIA1

General Information:
Name(s) found: RIA1 / YNL163C [SGD]
Description(s) found:
Found 25 descriptions.
SHOW ALL
  • Cytoplasmic GTPase involved in biogenesis of the 60S ribosome; has similarity to translation elongation factor 2 (Eft1p and Eft2p) [SGD]
    • Organism: Saccharomyces cerevisiae
    Length: 1110 amino acids

    Gene Ontology:
    Cellular Component: cytoplasm [IDALink to PubMed article]
    Biological Process: ribosomal large subunit biogenesis [IMPLink to PubMed article][IPILink to PubMed article][ISSLink to PubMed article]
    processing of 27S pre-rRNA [IMPLink to PubMed article]
    Molecular Function: GTPase activity [IDALink to PubMed article]


    Sequence:
    Sequence:
    [PDR BLAST]
    [ProtParam] 
          1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MPRVESETYK RLQNDPSCIR NICIVAHVDH GKTSLSDSLL ASNGIISQRL AGKIRFLDAR  60
   61 PDEQLRGITM ESSAISLYFR VLRKQEGSDE PLVSEHLVNL IDSPGHIDFS SEVSAASRLC 120
  121 DGAVVLVDVV EGVCSQTVTV LRQCWTEKLK PILVLNKIDR LITELQLTPQ EAYIHLSKVI 180
  181 EQVNSVIGSF FANERQLDDL FWREQLEKNE NAEYIEKDDS GIYFNPTDNN VIFASAIDGW 240
  241 GFNIGQLAKF YEQKLGAKRE NLQKVLWGDF YMDPKTKKII NNKGLKGRSL KPLFTSLILE 300
  301 NIWKIYQNII TSRDSEMVEK IAKTLNIKLL ARDLRSKDDK QLLRTIMGQW LPVSTAVLLT 360
  361 VIEKLPSPLE SQTDRLNTIL VSESDTAAMD PRLLKAMKTC DKEGPVSAYV SKMLSIPREE 420
  421 LPVESKRIAS SDELMERSRK AREEALNAAK HAGMVENMAM MDLNDNSKNT SDLYKRAKDT 480
  481 VMTPEVGEQT KPKPSRNNDV FCVVSEPSSA LDLEFEYEGE DDSDSQDNFG LDFVPTDIDP 540
  541 NDPLSSMFEY EEEDPLLESI KQISEDVNDE VDDIFDEKEE CLVAFARIYS GTLRVGQEIS 600
  601 VLGPKYDPKC PEEHIETAII THLYLFMGKE LVPLDVCPSG NIVGIRGLAG KVLKSGTLIE 660
  661 KGVQGVNLAG VNFHFTPIVR VAVEPANPVE MSKLVRGLKL LDQADPCVHT YVENTGEHIL 720
  721 CTAGELHLER CLKDLTERFA GIEITHSEPA IPYRETFLSA SDMNPPQNSQ LGRGVHELLL 780
  781 SQYKITFRTF PLSGKVTDFL SQHQNSIKNI LKTSTSSMDP VIESTGSSFL DKKSLLVAFE 840
  841 EVINQEEKSR ELLSGFKVKL AGFGPSRVGC NILLSQDNLL GSLFEGTPAA FEYSDSIKNG 900
  901 FQLAVSEGPL ANEPVQGMCV LVESVHKMSQ DEIESIEDPR YQQHIVDLSG RLITSTRDAI 960
  961 HEAFLDWSPR IMWAIYSCDI QTSVDVLGKV YAVILQRHGK IISEEMKEGT PFFQIEAHVP1020
 1021 VVEAFGLSED IRKRTSGAAQ PQLVFSGFEC IDLDPFWVPT TEEELEELGD TADRENIARK1080
 1081 HMNAIRRRKG LFIEEKVVEN AEKQRTLKKN

    Protein Complex Data

      PUBLICATION TOPOLOGY COCOMPLEXED PROTEINS
    View Details Riffle et al. (2010) (Unpublished Data) RIA1 SDO1
    View Details Krogan NJ, et al. (2006) KSP1 RIA1 SDO1
    View Details Qiu et al. (2008) HMS2 MNL1 RIA1 RKI1 RPL11B RPL12B, RPL12A RPL13A RPL17B RPL19B, RPL19A RPL1A, RPL1B RPL20B RPL21A RPL21B RPL24B RPL26A RPL27B RPL33A RPL33B RPL35A, RPL35B RPL37A RPL37B RPL41A, RPL41B RPL43B, RPL43A RPL6B RPL9B RPP1B RPP2A RPS16B, RPS16A RPS19A RPS19B RPS2 RPS21A RPS23B, RPS23A RPS24A, RPS24B RPS26A RPS26B RPS29B RPS30B, RPS30A RPS6A, RPS6B RPS7B TEF1, TEF2 TOS4 TSR1 YPL199C

    Mass Spectrometry Data

    The following runs contain data for this protein:

      BAIT COMMENTS PUBLICATION
    View Run CEP3 sample cbf3 from feb 2005 Sandall S, et all (2006)
    View Run RPA135 No Comments Schneider, DA, et al. (2006)
    View Run MLP2 #34 Asynchronous SPB prep Keck JM, et al. (2011)
    View Run MLP2 #32 Asynchronous Prep-No Phosphopeptide enrichment Keck JM, et al. (2011)
    View Run MLP2 #29 Asynchronous Prep5-TiO2 Flowthrough Keck JM, et al. (2011)
    View Run MLP2 #12 Mitotic Prep1-TiO2 Flowthrough Keck JM, et al. (2011)
    View Run MLP2 #30 Asynchronous Prep6-TiO2 Phosphopeptide enrichment Keck JM, et al. (2011)
    View Run MLP2 #16 Mitotic-New search criteria Keck JM, et al. (2011)
    View Run None Entered #18 Mitotic Prep4-TiO2 Phosphopeptide enrichment Keck JM, et al. (2011)

    Yeast Two-Hybrid Data

    The following interactions contain this protein:

    SHOWING SINGLE HITS. [ Hide Single Hits ]

    [View our yeast two-hybrid interpretation guidelines.]

      BAIT PREY HITS PUBLICATION
    View Screen UBC7 RIA1 1 Unpublished Fields Lab Data
    View Screen HRT1 RIA1 1 Unpublished Fields Lab Data

    Microscopy / Localization Data

    New Feature: Upload Your Own Microscopy Data

      PROTEIN(S) PUBLICATION
    View Data RIA1 Huh WK, et al. (2003)

    Protein Structure Data


    [What does the above image mean?]
    [View Top Sequence Alignments] [Show Ginzu Version Information]

    Domains predicted:

    #   Region(s) Method Confidence Match Description
    1 View Details [1..372] PSI-BLAST 282.0 Elongation factor G (EF-G), domain II; Elongation factor G (EF-G), N-terminal (G) domain; Elongation factor G (EF-G), domain IV; Elongation factor G (EF-G)
    2 View Details [373..528] PSI-BLAST 282.0 Elongation factor G (EF-G), domain II; Elongation factor G (EF-G), N-terminal (G) domain; Elongation factor G (EF-G), domain IV; Elongation factor G (EF-G)
    3 View Details [529..726] PSI-BLAST 282.0 Elongation factor G (EF-G), domain II; Elongation factor G (EF-G), N-terminal (G) domain; Elongation factor G (EF-G), domain IV; Elongation factor G (EF-G)
    4 View Details [727..974] ORFEUS 9.99 Elongation factor G (EF-G), domain II; Elongation factor G (EF-G), N-terminal (G) domain; Elongation factor G (EF-G), domain IV; Elongation factor G (EF-G)
    5 View Details [975..1047] PSI-BLAST 20.045757 70S ribosome functional complex
    6 View Details [1048..1110] MSA 1.055991 View MSA. No confident structure predictions are available.

    Functions predicted (by domain):

    # Gene Ontology predictions
    1 No functions predicted.
    2
    Term Confidence Notes
  • structural constituent of ribosome
    		•  4.9869754621766 bayes_pls_golite062009
  • structural molecule activity
    		•  4.395910578613 bayes_pls_golite062009
  • RNA binding
    		•  3.63527713952446 bayes_pls_golite062009
  • mRNA binding
    		•  2.41880219271933 bayes_pls_golite062009
  • translation elongation factor activity
    		•  2.39615611956381 bayes_pls_golite062009
  • 3'-5'-exoribonuclease activity
    		•  1.46861535960967 bayes_pls_golite062009
  • exoribonuclease activity, producing 5'-phosphomonoesters
    		•  1.24602568674916 bayes_pls_golite062009
  • transcription regulator activity
    		•  1.20748032530392 bayes_pls_golite062009
  • exoribonuclease activity
    		•  1.19859426598543 bayes_pls_golite062009
  • nucleic acid binding
    		•  1.13818950871382 bayes_pls_golite062009
  • binding
    		•  0.907487229190259 bayes_pls_golite062009
  • catalytic activity
    		•  0.878454819018611 bayes_pls_golite062009
  • ribonuclease activity
    		•  0.825588323751637 bayes_pls_golite062009
  • DNA binding
    		•  0.678913821280306 bayes_pls_golite062009
  • nuclease activity
    		•  0.29999921376873 bayes_pls_golite062009
  • tRNA-specific ribonuclease activity
    		•  0.249659703226717 bayes_pls_golite062009
  • transferase activity, transferring phosphorus-containing groups
    		•  0.1757578095579 bayes_pls_golite062009
    3 No functions predicted.
    4 No functions predicted.
    5 No functions predicted.
    6 No functions predicted.


    Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076


    Philius Transmembrane Prediction:

    Protein predicted to be: GLOBULAR (No transmembrane regions or signal peptide)
    Confidence of classification: 0.95

    Source: Reynolds et al. (2008)

    YRC Informatics Platform - Version 3.0
    Created and Maintained by: Michael Riffle