Protein Overview: BDF1

General Information:
Name(s) found:	BDF1 / YLR399C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6323431\|ref\|NP_013503.1\| Protein involved in transcription initiation at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with [NCBI NR] gi\|625115\|gb\|AAB82357.1\| Bdf1p [Saccharomyces cerevisiae] [NCBI NR] gi\|5921175\|sp\|P35817.3\|BDF1_YEAST RecName: Full=Bromodomain-containing factor 1 [NCBI NR] pir\|\|S55955 bromodomain protein BDF1 - yeast (Saccharomyces cerevisiae) [NCBI NR] Bromodomain-containing factor 1 OS=Saccharomyces cerevisiae GN=BDF1 PE=1 SV=3 [Swiss-Prot] BDF1 Sporulation protein [mips] Protein involved in transcription initiation at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf2p [SGD] BDF1, Chr XII from 919535-921595, reverse complement [yeast_orfs2] Chr XII from 919535-921595, reverse complement [yeastorf3.fasta] Chr XII from 921595-919535, reverse complement [yeastorf5.fasta] (P35817) BDF1 protein [4932.FASTACCERV.txt] BDF1 SGDID:S0004391, Chr XII from 921596-919536, reverse complement, Verified ORF [yeastflysequest.fasta] Bdf1p [Saccharomyces cerevisiae]gi\|5921175\|sp\|P35817\|BDF1_YEAST BDF1 proteingi\|1084645\|pir\|\|S55955 bromodomain protein BDF1 - yeast (Saccharomyces cerevisiae)gi\|625115\|gb\|AAB82357.1\| Bdf1p [Saccharomyces cerevisiae] [nrdb_11152004_Chlamydomonas] BDF1 SGDID:S000004391, Chr XII from 921596-919536, reverse complement, Verified ORF, "Protein involved in transcription initiation at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds t [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] Bdf1p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Protein involved in transcription initiation at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf2p; Bdf1p [Saccharomyces [nr-271106-contam.fasta] Bromodomain-containing factor 1 [nr-271106-contam.fasta] BDF1 SGDID:S000004391, Chr XII from 921596-919536, reverse complement, Verified ORF, "Protein involved in transcription initiation at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf2p" [yeast-bovrabprots.fasta] [chemostatdb.fasta] Bromodomain-containing factor 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=BDF1 PE=1 SV=3 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] BDF1 SGDID:S000004391, Chr XII from 921596-919536, Genome Release 64-1-1, reverse complement, Verified ORF, "Protein involved in transcription initiation at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf2p" [yeast-030211-contam.fasta] BDF1 SGDID:S000004391, Chr XII from 921596-919536, Genome Release 64-2-1, reverse complement, Verified ORF, "Protein involved in transcription initiation; functions at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf2p; BDF1 has a paralog, BDF2, that arose from the whole genome duplication" [20141208_orf_trans.fasta] TR7090\|c0_g1_i1\|g.4934 ORF TR7090\|c0_g1_i1\|g.4934 TR7090\|c0_g1_i1\|m.4934 type:complete len:687 (+) TR7090\|c0_g1_i1:48-2108(+) [Y12_S_cerevisiae-Sep2015-contam.fasta] YLR399C SGDID:S000004391, Chr XII from 921596-919536, Genome Release 64-1-1, reverse complement, Verified ORF, "Protein involved in transcription initiation at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf2p" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta] asmbl_7764\|g.5564 ORF asmbl_7764\|g.5564 asmbl_7764\|m.5564 type:complete len:687 (+) asmbl_7764:59-2119(+) [Y12_Trinity_v2_2_0_PASA_6May2016-contam.fasta] ID=asmbl_7542\|g.4746 ORF ID=asmbl_7542\|g.4746 ID=asmbl_7542\|m.4746 type:complete len:687 (+) ID=asmbl_7542:59-2119(+) [Y12_quiver_Trinity_v2_2_0_PASA_2June2016-contam.fasta]
Organism:	Saccharomyces cerevisiae
Length:	686 amino acids
Gene Ontology:
Cellular Component:	nucleus [IDA] nuclear chromatin [IDA]
Biological Process:	ascospore formation [IMP] DNA repair [IMP] chromatin remodeling [IPI] meiosis [IMP]
Molecular Function:	transcription regulator activity [IMP][ISS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MTDITPVQND VDVNGNNVND DVSSNLKRPI DQGDPSNGLA EEENPANNQL HLKKARLDGD  60
   61 ALTSSPAGLA ENGIEGATLA ANGENGYNAT GSGAEDEQQG LKKEEGGQGT KQEDLDENSK 120
  121 QELPMEVPKE PAPAPPPEPD MNNLPQNPIP KHQQKHALLA IKAVKRLKDA RPFLQPVDPV 180
  181 KLDIPFYFNY IKRPMDLSTI ERKLNVGAYE VPEQITEDFN LMVNNSIKFN GPNAGISQMA 240
  241 RNIQASFEKH MLNMPAKDAP PVIAKGRRSS AQEDAPIVIR RAQTHNGRPK RTIHPPKSKD 300
  301 IYPYESKKPK SKRLQQAMKF CQSVLKELMA KKHASYNYPF LEPVDPVSMN LPTYFDYVKE 360
  361 PMDLGTIAKK LNDWQYQTME DFERDVRLVF KNCYTFNPDG TIVNMMGHRL EEVFNSKWAD 420
  421 RPNLDDYDSD EDSRTQGDYD DYESEYSESD IDETIITNPA IQYLEEQLAR MKVELQQLKK 480
  481 QELEKIRKER RLARGSKKRG KRSKGRSGSK NASSKGRRDK KNKLKTVVTY DMKRIITERI 540
  541 NDLPTSKLER AIDIIKKSMP NISEDDEVEL DLDTLDNHTI LTLYNTFFRQ YESSSGASNG 600
  601 LDGTSGVTRD ASSLSPTSAG SRKRRSKALS QEEQSRQIEK IKNKLAILDS ASPLSQNGSP 660
  661 GQIQSAAHNG FSSSSDDDVS SESEEE

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		ARP4 ARP6 BDF1 RVB1 RVB2 SWC3 SWC4 SWC5 SWC7 SWR1 YAF9 YNG2
View Details	Krogan NJ, et al. (2006)		BDF1 BDF2
View Details	Qiu et al. (2008)		BDF1 TAF14

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	LST8	No Comments	Reinke A, et al. (2004)
View Run	MLP2	#12 Mitotic Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#06 Alpha Factor Prep3-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	BDF1	INP51	2	Unpublished Fields Lab Data
View Screen	BDF1	SUI1	2	Unpublished Fields Lab Data
View Screen	BDF1	YOR338W	2	Unpublished Fields Lab Data
View Screen	SGT1	BDF1	2	Unpublished Fields Lab Data
View Screen	SGT1	BDF1	2	Unpublished Fields Lab Data
View Screen	SGT1	BDF1	2	Unpublished Fields Lab Data
View Screen	SGT1	BDF1	2	Unpublished Fields Lab Data
View Screen	SKP1	BDF1	2	Uetz P, et al. (2000)
View Screen	SGT1	BDF1	2	Unpublished Fields Lab Data
View Screen	SKP1	BDF1	2	Unpublished Fields Lab Data
View Screen	SKP1	BDF1	2	Unpublished Fields Lab Data
View Screen	SKP1	BDF1	2	Unpublished Fields Lab Data
View Screen	SKP1	BDF1	2	Unpublished Fields Lab Data
View Screen	BDF1	STE14	2	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	BDF1	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..151]	ab initio	7.25	No description for 1ldvA was found.
2	View Details	[152..269]	Pfam	31.259637	Bromodomain Confident ab initio structure predictions are available.
3	View Details	[270..472]	PSI-BLAST	176.38764	GCN5
4	View Details	[473..527]	deduced	N/A	No confident structure predictions are available.
5	View Details	[528..686]	MSA	3.056989	View MSA. No confident structure predictions are available.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2