Protein Overview: NUP85

General Information:
Name(s) found:	NUP85 / YJR042W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6322502\|ref\|NP_012576.1\| Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC), required for assembly of the subcomplex and also for formation of the nucleocytoplasmic Gsp1p concentration gradient that plays a role in nuclear trafficking; [NCBI NR] gi\|1197070\|gb\|AAA88744.1\| ORF; putative [Saccharomyces cerevisiae] [NCBI NR] gi\|1177644\|emb\|CAA62481.1\| Nup85p [Saccharomyces cerevisiae] [NCBI NR] gi\|1171854\|sp\|P46673.1\|NUP85_YEAST RecName: Full=Nucleoporin NUP85; AltName: Full=Nuclear pore protein NUP85 [NCBI NR] pir\|\|S57061 nuclear pore protein NUP85 - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|1015695\|emb\|CAA89569.1\| NUP85 [Saccharomyces cerevisiae] [NCBI NR] Nucleoporin NUP85 OS=Saccharomyces cerevisiae GN=NUP85 PE=1 SV=1 [Swiss-Prot] (P46673) Nucleoporin NUP85 (Nuclear pore protein NUP85) [Swiss-Prot] NUP85 Nuclear pore protein [mips] Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC), required for assembly of the subcomplex and also for formation of the nucleocytoplasmic Gsp1p concentration gradient that plays a role in nuclear trafficking [SGD] NUP85, Chr X from 513747-515981 [yeast_orfs2] Chr X from 513747-515981 [yeastorf3.fasta] Chr X from 513748-515982 [yeastorf4.fasta] Chr X from 513749-515983 [yeastorf5.fasta] NUP85 SGDID:S0003803, Chr X from 513969-516203, Verified ORF [yeastflysequest.fasta] Nup85p [Saccharomyces cerevisiae]gi\|1015695\|emb\|CAA89569.1\| NUP85 [Saccharomyces cerevisiae]gi\|1177644\|emb\|CAA62481.1\| Nup85p [Saccharomyces cerevisiae]gi\|1078265\|pir\|\|S57061 nuclear pore protein NUP85 - yeast (Saccharomyces cerevisiae)gi\|1197070\|gb\|A [nrdb_11152004_Chlamydomonas] NUP85 SGDID:S000003803, Chr X from 513969-516203, Verified ORF, "Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC), required for assembly of the subcomplex and also for formation of the nucleocytoplasmic Gsp1p concentration gradient that [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] NUP85 SGDID:S000003803, Chr X from 514048-516282, Verified ORF, "Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC), required for assembly of the subcomplex and also for formation of the nucleocytoplasmic Gsp1p concentration gradient that [yeast-contam.fasta] NUP85 SGDID:S000003803, Chr X from 514047-516281, Verified ORF, "Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC), required for assembly of the subcomplex and also for formation of the nucleocytoplasmic Gsp1p concentration gradient that [yeast-200209-contam.fasta] Nucleoporin NUP85 (Nuclear pore protein NUP85) [nr-271106-contam.fasta] Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC), required for assembly of the subcomplex and also for formation of the nucleocytoplasmic Gsp1p concentration gradient that plays a role in nuclear trafficking; Nup85p [Saccharomyces cerevi [nr-271106-contam.fasta] ORF; putative [nr-271106-contam.fasta] NUP85 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Nup85p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] NUP85 SGDID:S000003803, Chr X from 514047-516281, Verified ORF, "Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC), required for assembly of the subcomplex and also for formation of the nucleocytoplasmic Gsp1p concentration gradient that plays a role in nuclear trafficking" [yeast-bovrabprots.fasta] NUP85 SGDID:S000003803, Chr X from 514048-516282, Verified ORF, "Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC), required for assembly of the subcomplex and also for formation of the nucleocytoplasmic Gsp1p concentration gradient that plays a role in nuclear trafficking" [yeast-contam_custom.fasta] [chemostatdb.fasta] Nucleoporin NUP85 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=NUP85 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] NUP85 SGDID:S000003803, Chr X from 514055-516289, Genome Release 64-1-1, Verified ORF, "Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); required for assembly of the subcomplex and also for formation of the nucleocytoplasmic Gsp1p concentration gradient that plays a role in nuclear trafficking; the Nup84 subcomplex has a role in transcription elongation" [yeast-030211-contam.fasta] NUP85 SGDID:S000003803, Chr X from 514055-516289, Genome Release 64-2-1, Verified ORF, "Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP85 aka NUP75" [20141208_orf_trans.fasta] YJR042W SGDID:S000003803, Chr X from 514055-516289, Genome Release 64-1-1, Verified ORF, "Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); required for assembly of the subcomplex and also for formation of the nucleocytoplasmic Gsp1p concentration gradient that plays a role in nuclear trafficking; the Nup84 subcomplex has a role in transcription elongation" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	744 amino acids
Gene Ontology:
Cellular Component:	nuclear pore [IDA]
Biological Process:	mRNA export from nucleus [IMP] rRNA export from nucleus [TAS] nuclear envelope organization [IMP] nuclear pore organization [TAS] snRNP protein import into nucleus [TAS] mRNA-binding (hnRNP) protein import into nucleus [TAS] snRNA export from nucleus [TAS] tRNA export from nucleus [TAS] NLS-bearing substrate import into nucleus [TAS] protein export from nucleus [TAS] ribosomal protein import into nucleus [TAS]
Molecular Function:	structural molecule activity [TAS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MTIDDSNRLL MDVDQFDFLD DGTAQLSNNK TDEEEQLYKR DPVSGAILVP MTVNDQPIEK  60
   61 NGDKMPLKFK LGPLSYQNMA FITAKDKYKL YPVRIPRLDT SKEFSAYVSG LFEIYRDLGD 120
  121 DRVFNVPTIG VVNSNFAKEH NATVNLAMEA ILNELEVFIG RVKDQDGRVN RFYELEESLT 180
  181 VLNCLRTMYF ILDGQDVEEN RSEFIESLLN WINRSDGEPD EEYIEQVFSV KDSTAGKKVF 240
  241 ETQYFWKLLN QLVLRGLLSQ AIGCIERSDL LPYLSDTCAV SFDAVSDSIE LLKQYPKDSS 300
  301 STFREWKNLV LKLSQAFGSS ATDISGELRD YIEDFLLVIG GNQRKILQYS RTWYESFCGF 360
  361 LLYYIPSLEL SAEYLQMSLE ANVVDITNDW EQPCVDIISG KIHSILPVME SLDSCTAAFT 420
  421 AMICEAKGLI ENIFEGEKNS DDYSNEDNEM LEDLFSYRNG MASYMLNSFA FELCSLGDKE 480
  481 LWPVAIGLIA LSATGTRSAK KMVIAELLPH YPFVTNDDIE WMLSICVEWR LPEIAKEIYT 540
  541 TLGNQMLSAH NIIESIANFS RAGKYELVKS YSWLLFEASC MEGQKLDDPV LNAIVSKNSP 600
  601 AEDDVIIPQD ILDCVVTNSM RQTLAPYAVL SQFYELRDRE DWGQALRLLL LLIEFPYLPK 660
  661 HYLVLLVAKF LYPIFLLDDK KLMDEDSVAT VIEVIETKWD DADEKSSNLY ETIIEADKSL 720
  721 PSSMATLLKN LRKKLNFKLC QAFM

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		NUP120 NUP145 NUP84 NUP85 SEC13 SEH1
View Details	(MIPS) Mewes HW, et al. (2004)		NUP120 NUP145 NUP84 NUP85 SEC13 SEH1
View Details	Krogan NJ, et al. (2006)		MEX67 NUP120 NUP145 NUP84 NUP85 SEH1
View Details	Gavin AC, et al. (2006)		NUP120 NUP145 NUP84 NUP85 SEC13 SEH1
View Details	Gavin AC, et al. (2002)		CKA1 CKA2 CKB1 DBP2 MRPS28 NUP120 NUP145 NUP84 NUP85 RPP2B SEC13 SEC16 SEC23 SEC31 SEH1 STM1
View Details	Ho Y, et al. (2002)		ADE13 CBP3 HEM15 NUP145 NUP84 NUP85 SEC13 SEH1 YMR209C
View Details	Qiu et al. (2008)		GLE2 KAP120 NUP1 NUP100 NUP120 NUP133 NUP145 NUP157 NUP159 NUP170 NUP188 NUP2 NUP42 NUP82 NUP84 NUP85 POM152 SEC13 SEH1

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	ZDS1	Sample 2- cpn2 from june 2005	McCusker D, et al (2007)
View Run	ZDS1	Sample 3 - cpn + atp, from june 2005	McCusker D, et al (2007)
View Run	MLP2	#32 Asynchronous Prep-No Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#12 Mitotic Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

No yeast two-hybrid interactions found for this protein.

	PROTEIN(S)	PUBLICATION
View Data	NUP85	Huh WK, et al. (2003)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..134]	deduced	N/A	No confident structure predictions are available.
2	View Details	[135..678]	MSA	2.008904	View MSA. No confident structure predictions are available.
3	View Details	[679..744]	deduced	N/A	No confident structure predictions are available.

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.96

Protein Overview: NUP85

Protein Complex Data

Mass Spectrometry Data

Yeast Two-Hybrid Data

Microscopy / Localization Data

Protein Structure Data

Philius Transmembrane Prediction: