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Protein Overview: NUT1

General Information:
Name(s) found: NUT1 / YGL151W [SGD]
Description(s) found:
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  • gi|6321287|ref|NP_011364.1| Component of the RNA polymerase II mediator complex, which is required for transcriptional activation and also has a role in basal transcription; Nut1p [Saccharomyces cerevisiae] [NCBI NR]
  • pir||S60433 probable membrane protein YGL151w - yeast (Saccharomyces cerevisiae) [NCBI NR]
  • gi|1723935|sp|P53114.1|MED5_YEAST RecName: Full=Mediator of RNA polymerase II transcription subunit 5; AltName: Full=Mediator complex subunit 5; AltName: Full=Negative regulator of URS2 protein 1 [NCBI NR]
  • gi|1322737|emb|CAA96863.1| NUT1 [Saccharomyces cerevisiae] [NCBI NR]
  • (P53114) NUT1 protein [Swiss-Prot]
  • Mediator of RNA polymerase II transcription subunit 5 OS=Saccharomyces cerevisiae GN=NUT1 PE=1 SV=1 [Swiss-Prot]
  • NUT1 Negative transcription regulator from artifical reporters [mips]
  • Component of the RNA polymerase II mediator complex, which is required for transcriptional activation and also has a role in basal transcription [SGD]
  • NUT1, Chr VII from 217527-220925 [yeast_orfs2]
  • Chr VII from 217527-220925 [yeastorf3.fasta]
  • NUT1 SGDID:S0003119, Chr VII from 217527-220925, Verified ORF [yeastflysequest.fasta]
  • NUT1 SGDID:S0003119, Chr VII from 217529-220927, Verified ORF [10112005cervSGD.fasta]
  • Component of the RNA polymerase II mediator complex, which is required for transcriptional activation and also has a role in basal transcription [Saccharomyces cerevisiae]gi|1322737|emb|CAA96863.1| NUT1 [Saccharomyces cerevisiae]gi|2132555|pir||S60433 p [nrdb_11152004_Chlamydomonas]
  • NUT1 SGDID:S000003119, Chr VII from 217529-220927, Verified ORF, "Component of the RNA polymerase II mediator complex, which is required for transcriptional activation and also has a role in basal transcription" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta]
  • NUT1 [Saccharomyces cerevisiae] [nr-271106-contam.fasta]
  • RNA polymerase II mediator complex subunit 5 (Negative regulator of URS2 protein 1) [nr-271106-contam.fasta]
  • Component of the RNA polymerase II mediator complex, which is required for transcriptional activation and also has a role in basal transcription; Nut1p [Saccharomyces cerevisiae] [nr-271106-contam.fasta]
  • [chemostatdb.fasta]
  • Mediator of RNA polymerase II transcription subunit 5 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=NUT1 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta]
  • NUT1 SGDID:S000003119, Chr VII from 217524-220922, Genome Release 64-1-1, Verified ORF, "Component of the RNA polymerase II mediator complex, which is required for transcriptional activation and also has a role in basal transcription" [yeast-030211-contam.fasta]
  • NUT1 SGDID:S000003119, Chr VII from 217524-220922, Genome Release 64-2-1, Verified ORF, "Component of the RNA polymerase II mediator complex; mediator is required for transcriptional activation and also has a role in basal transcription" [20141208_orf_trans.fasta]
  • YGL151W SGDID:S000003119, Chr VII from 217524-220922, Genome Release 64-1-1, Verified ORF, "Component of the RNA polymerase II mediator complex, which is required for transcriptional activation and also has a role in basal transcription" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
    • Organism: Saccharomyces cerevisiae
    Length: 1132 amino acids

    Gene Ontology:
    Cellular Component: nucleus [IDALink to PubMed article]
    mitochondrion [IDALink to PubMed article]
    Biological Process: regulation of transcription from RNA polymerase II promoter [IMPLink to PubMed article]
    Molecular Function: molecular_function [ND]


    Sequence:
    Sequence:
    [PDR BLAST]
    [ProtParam] 
          1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MEKESVYNLA LKCAERQLTS MEFSNLYKEF FNEKFPSLIQ EEEEDTTTTA NINEVKKASD  60
   61 LVDTPSNNTA ATADTTHLHE ALDIVCSDFV KILNLEKPLI LADYIVEVLL VNYNSDMIKC 120
  121 FLPKLNSVRN SLLLAHFFSK SCSFFAKLSD TLIIDQVRKD LGNVIVPNIL SLDMNSMNKE 180
  181 LIAIVSKLLQ TTLKLSPSPI LLTSAGCKNG SFTLLNQLSQ TNKLLFKRVS QTFEAKLHFK 240
  241 DTKPFLNKDS TNEFVGSPSL TSPQYIPSPL SSTKPPGSVN SAAKYKDMKL LRYYKNIWLN 300
  301 NKIINWEISN PDFLSKYSAI TSSIFQESFN SVQNLDQLLT DLIETSFTCF AQFVSNKQYH 360
  361 QANSNLTLLE RKWVIFITKH LPLLILENSS RSPRVVTNAL DNIDEKVVKA IRIYFTEKDD 420
  421 NKTNNEDLFD DYPSTSLDIR HDFIKGLIML NLQPASVINN YLREDQMIDT SILPTRDDLF 480
  481 VRNLQGIQEV VHNTNSFIIS SLDTLELESI TESITHDSSN GLFQVLHNFE SVAPTKQREI 540
  541 VKAFLSIFED AIKELNYNRI AKICALLFFN FSHSLTTILS FSSPAALMKT LIKFVDLSRN 600
  601 GRNGSNGNDE SSEYETINIS LSFSWAILLI INLTQTYGIS VVDVALKYPE LSIKNSFIIN 660
  661 FISNLPNVSD KYYLEESNVN DSDMLTKSHN TVQSWLCDLF VNGSITDQLI QNIETRQLAN 720
  721 LIPFIVKQVL LSVEIGVLTD ISSLIGGFEY FLQPLLLVGL IKTFYWLEQF LSCVKNDTIS 780
  781 EDILQGIFNL LNTLFNPVTL NEDSKAFHTA VLRLNAIPLL KVLRKFRVQS QSNYGIYSSD 840
  841 AQGDPNLEPL IAKLVAVLNV SPVYDVDPRI INSENDYSRK QLGYGKFLIL NENPINKIMT 900
  901 NQINSFWSLH SSTYYNLDYL FELIELVTPK SFLFDVLKTL EYKLATYGVP GSENKRGSLD 960
  961 SEHVFDYFFY FLVLYDVKTA EEASQLIEYM ENDAKKSKGD VDIKGEDLHE KNDSAEVRQE1020
 1021 TQPKAEATQD DDFDMLFGEN DTSTQAYEEE EENEDNDGNN RTNNVPMIKA EETPSKTNKI1080
 1081 SILKRHSFAV LLHERKLLND LALENGEITK TENEKFISYH DKYLCMLKTC VF

    Protein Complex Data

      PUBLICATION TOPOLOGY COCOMPLEXED PROTEINS
    View Details Riffle et al. (2010) (Unpublished Data) ADA2 CSE2 GAL11 MED1 MED11 MED4 MED6 MED7 MED8 NUT1 NUT2 PGD1 RGR1 SIN4 SRB2 SRB4 SRB5 SRB6 SRB7 SRB8 SSN2 SSN3 YAP1
    View Details (MIPS) Mewes HW, et al. (2004) CSE2 DMC1 GAL11 MED11 MED2 MED4 MED6 MED7 MED8 NUT1 NUT2 PGD1 RGR1 ROX3 SIN4 SRB2 SRB4 SRB5 SRB6 SRB7 TAF14
    View Details Krogan NJ, et al. (2006) CSE2 DSS1 GAL11 GPT2 IRC11 MED1 MED11 MED2 MED4 MED6 MED7 MED8 NUT1 NUT2 PGD1 RGR1 ROX3 SIN4 SOH1 SRB2 SRB4 SRB5 SRB6 SRB7 SSN3 SSN8 THI72
    View Details Gavin AC, et al. (2006) CSE2 GAL11 MED1 MED11 MED6 MED7 MED8 NUT1 NUT2 PGD1 RGR1 SIN4 SRB2 SRB4 SRB5 SRB6 SRB7 SRB8 SSN2
    View Details Gavin AC, et al. (2002) ADA2 ADH1 COG3 CSE2 ENO2 FAB1 FBA1 GAL11 GCN5 MED1 MED11 MED2 MED4 MED6 MED7 MED8 MLP1 MRPL6 NUT1 NUT2 PGD1 PGK1 RGR1 ROX3 RPG1 RPO21 SIN4 SPT7 SRB2 SRB4 SRB5 SRB6 SRB7 SRB8 SRP1 SSE2 SSN2 SSN3 SSN8 TAF6 TIF1, TIF2 YIL077C
    View Details Ho Y, et al. (2002) BFR2 BIR1 BNA5 CDC7 ECM10 JIP3 NUT1 PDC5 PDC6 PST2 RPC19 SAR1 SEC27 STI1 THI3 TPS1 UBI4 VAC14
    View Details Qiu et al. (2008) ADA2 CSE2 GAL11 MED1 MED11 MED2 MED4 MED6 MED7 MED8 NUT1 NUT2 PGD1 RGR1 SGF73 SIN4 SOH1 SPT20 SRB2 SRB4 SRB5 SRB6 SRB7 TAF10 TAF12 TAF14 TAF3

    Mass Spectrometry Data

    The following runs contain data for this protein:

      BAIT COMMENTS PUBLICATION
    View Run ZDS1 Sample 2- cpn2 from june 2005 McCusker D, et al (2007)
    View Run MLP2 #12 Mitotic Prep1-TiO2 Flowthrough Keck JM, et al. (2011)

    Yeast Two-Hybrid Data

    The following interactions contain this protein:

    NOT SHOWING SINGLE HITS. [ Show Single Hits ]

    [View our yeast two-hybrid interpretation guidelines.]

    No yeast two-hybrid interactions found for this protein.

    Microscopy / Localization Data

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      PROTEIN(S) PUBLICATION
    View Data NUT1 Huh WK, et al. (2003)

    Protein Structure Data


    [What does the above image mean?]
    [View Top Sequence Alignments] [Show Ginzu Version Information]

    Domains predicted:

    #   Region(s) Method Confidence Match Description
    1 View Details [1..1132] deduced N/A No confident structure predictions are available.


    Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076


    Philius Transmembrane Prediction:

    Protein predicted to be: GLOBULAR (No transmembrane regions or signal peptide)
    Confidence of classification: 0.89

    Source: Reynolds et al. (2008)

    YRC Informatics Platform - Version 3.0
    Created and Maintained by: Michael Riffle