Bait Protein: | MLP2 |
Organism: | Saccharomyces cerevisiae |
Publication: | Keck JM, Jones MH, Wong CC, Binkley J, Chen D, Jaspersen SL, Holinger EP, Xu T, Niepel M, Rout MP, Vogel J, Sidow A, Yates JR, Winey M., A Cell Cycle Phosphoproteome of the Yeast Centrosome, Science 24 June 2011: 1557-1561. |
Comments: | #25 Asynchronous Prep3-TiO2 Flowthrough |
Raw Data: |
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FILTERED Run Results:
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Hit Protein | Protein Desc | Sequence Count | Spectrum Count | Sequence Coverage | NSAF | Mol. Wt. | |
[ |
SNU13 | RNA binding protein, part of U3 snoRNP involved in rRNA processing, part of U4/U6-U5 tri-snRNP invol... | 3 | 7 | 49.2% | 0.0127 | 13569 |
[ |
PDC1 | Major of three pyruvate decarboxylase isozymes, key enzyme in alcoholic fermentation, decarboxylates... | 18 | 93 | 44.4% | 0.0379 | 61495 |
[ |
HHT1, HHT2 | Histone H3, core histone protein required for chromatin assembly, part of heterochromatin-mediated t... | 4 | 12 | 37.5% | 0.0202 | 15356 |
[ |
ENO2 | Enolase II, a phosphopyruvate hydratase that catalyzes the conversion of 2-phosphoglycerate to phosp... | 14 | 40 | 35.0% | 0.021 | 46914 |
[ |
CMD1 | Calmodulin; Ca++ binding protein that regulates Ca++ independent processes (mitosis, bud growth, act... | 2 | 22 | 34.0% | 0.0343 | 16135 |
[ |
CDC19 | Pyruvate kinase, functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvat... | 13 | 55 | 33.8% | 0.0252 | 54545 |
[ |
HTB1 | Histone H2B, core histone protein required for chromatin assembly and chromosome function; nearly id... | 3 | 4 | 32.1% | 0.00701 | 14252 |
[ |
HTB2 | Histone H2B, core histone protein required for chromatin assembly and chromosome function; nearly id... | 3 | 4 | 32.1% | 0.00701 | 14237 |
[ |
HTA1 | Histone H2A, core histone protein required for chromatin assembly and chromosome function; one of tw... | 3 | 10 | 28.8% | 0.0174 | 13989 |
[ |
HTA2 | Histone H2A, core histone protein required for chromatin assembly and chromosome function; one of tw... | 3 | 10 | 28.8% | 0.0174 | 13989 |
[ |
ASC1 | G-protein beta subunit and guanine nucleotide dissociation inhibitor for Gpa2p; ortholog of RACK1 th... | 5 | 10 | 26.0% | 0.00719 | 34805 |
[ |
MLP2 | Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the... | 40 | 103 | 24.9% | 0.0141 | 195140 |
[ |
EFT2, EFT1 | Elongation factor 2 (EF-2), also encoded by EFT1; catalyzes ribosomal translocation during protein s... | 11 | 16 | 23.5% | 0.00436 | 93289 |
[ |
ADH1 | Alcohol dehydrogenase, fermentative isozyme active as homo- or heterotetramers; required for the red... | 5 | 13 | 21.0% | 0.00857 | 36849 |
[ |
FBA1 | Fructose 1,6-bisphosphate aldolase, required for glycolysis and gluconeogenesis; catalyzes conversio... | 5 | 14 | 20.3% | 0.00895 | 39621 |
[ |
TEF1, TEF2 | Translational elongation factor EF-1 alpha; also encoded by TEF2; functions in the binding reaction ... | 12 | 35 | 20.1% | 0.0175 | 50033 |
[ |
HHF1, HHF2 | Histone H4, core histone protein required for chromatin assembly and chromosome function; one of two... | 2 | 4 | 19.4% | 0.00891 | 11368 |
[ |
YDJ1 | Protein chaperone involved in regulation of the HSP90 and HSP70 functions; involved in protein trans... | 2 | 4 | 19.3% | 0.00224 | 44671 |
[ |
PGK1 | 3-phosphoglycerate kinase, catalyzes transfer of high-energy phosphoryl groups from the acyl phospha... | 6 | 11 | 19.0% | 0.00607 | 44738 |
[ |
BMH1 | 14-3-3 protein, major isoform; controls proteome at post-transcriptional level, binds proteins and D... | 2 | 3 | 18.4% | 0.00258 | 30091 |
[ |
SPC29 | Inner plaque spindle pole body (SPB) component, links the central plaque component Spc42p to the inn... | 4 | 14 | 18.2% | 0.0127 | 29280 |
[ |
BMH2 | 14-3-3 protein, minor isoform; controls proteome at post-transcriptional level, binds proteins and D... | 2 | 3 | 17.9% | 0.00252 | 31061 |
[ |
TPI1 | Triose phosphate isomerase, abundant glycolytic enzyme; mRNA half-life is regulated by iron availabi... | 2 | 3 | 17.7% | 0.00278 | 26795 |
[ |
SPC42 | Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate att... | 4 | 21 | 17.4% | 0.0133 | 42271 |
[ |
RPP0 | Conserved ribosomal protein P0 similar to rat P0, human P0, and E. coli L10e; shown to be phosphoryl... | 3 | 5 | 16.3% | 0.00368 | 33717 |
[ |
CNM67 | Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclea... | 11 | 40 | 16.2% | 0.0158 | 67400 |
[ |
PGI1 | Glycolytic enzyme phosphoglucose isomerase, catalyzes the interconversion of glucose-6-phosphate and... | 2 | 4 | 15.7% | 0.00166 | 61299 |
[ |
CDC14 | Protein phosphatase required for mitotic exit; located in the nucleolus until liberated by the FEAR ... | 5 | 12 | 15.6% | 0.005 | 61907 |
[ |
HOM6 | Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase), dimeric enzyme that catalyzes the third... | 2 | 2 | 15.6% | 0.00128 | 38502 |
[ |
CDC31 | Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication i... | 2 | 6 | 14.3% | 0.00855 | 18751 |
[ |
TSA1 | Thioredoxin peroxidase, acts as both a ribosome-associated and free cytoplasmic antioxidant; self-as... | 2 | 3 | 13.8% | 0.00351 | 21590 |
[ |
SPC110 | Inner plaque spindle pole body (SPB) component, ortholog of human kendrin; involved in connecting nu... | 10 | 22 | 12.5% | 0.00535 | 111782 |
[ |
ACT1 | Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functio... | 2 | 3 | 12.3% | 0.00184 | 41690 |
[ |
PMA1 | Plasma membrane H+-ATPase, pumps protons out of the cell; major regulator of cytoplasmic pH and plas... | 8 | 13 | 12.0% | 0.00325 | 99619 |
[ |
MET17 | Methionine and cysteine synthase (O-acetyl homoserine-O-acetyl serine sulfhydrylase), required for s... | 3 | 6 | 11.7% | 0.0031 | 48672 |
[ |
GPM1 | Tetrameric phosphoglycerate mutase, mediates the conversion of 3-phosphoglycerate to 2-phosphoglycer... | 2 | 3 | 11.7% | 0.00279 | 27609 |
[ |
SPC98 | Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the... | 4 | 6 | 10.6% | 0.00163 | 98227 |
[ |
RNR2 | Ribonucleotide-diphosphate reductase (RNR), small subunit; the RNR complex catalyzes the rate-limiti... | 2 | 3 | 10.0% | 0.00173 | 46147 |
[ |
YDR261C-D | Retrotransposon TYA Gag and TYB Pol genes; in YDRCTY1-3 TYB is mutant and probably non-functional | 10 | 20 | 9.4% | 0.00286 | 181660 |
[ |
RFC1 | Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase t... | 2 | 2 | 9.1% | 5.33E-4 | 94903 |
[ |
NUD1 | Component of the spindle pole body outer plaque, required for exit from mitosis | 4 | 13 | 8.9% | 0.00351 | 94104 |
[ |
YDR365W-B | Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... | 10 | 20 | 8.5% | 0.00261 | 198658 |
[ |
YDR098C-B | Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... | 10 | 20 | 8.5% | 0.00261 | 198594 |
[ |
YDR210C-D | Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... | 10 | 20 | 8.5% | 0.00261 | 198838 |
[ |
YDR316W-B | Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... | 10 | 20 | 8.5% | 0.00261 | 198526 |
[ |
YER138C | Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... | 10 | 20 | 8.5% | 0.00261 | 198561 |
[ |
NUP60 | Subunit of the nuclear pore complex (NPC), functions to anchor Nup2p to the NPC in a process control... | 2 | 2 | 8.5% | 8.51E-4 | 59039 |
[ |
YHB1 | Nitric oxide oxidoreductase, flavohemoglobin involved in nitric oxide detoxification; plays a role i... | 3 | 8 | 8.5% | 0.0046 | 44646 |
[ |
YEF3 | Translational elongation factor 3, stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes b... | 6 | 14 | 8.2% | 0.00308 | 115945 |
[ |
VMA2 | Subunit B of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), a... | 2 | 3 | 7.2% | 0.00133 | 57749 |
[ |
URA7 | Major CTP synthase isozyme (see also URA8), catalyzes the ATP-dependent transfer of the amide nitrog... | 2 | 2 | 6.6% | 7.93E-4 | 64710 |
[ |
MRM1 | Ribose methyltransferase that modifies a functionally critical, conserved nucleotide in mitochondria... | 2 | 5 | 6.3% | 0.00278 | 46387 |
[ |
ATP2 | Beta subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily c... | 2 | 7 | 5.7% | 0.00314 | 54794 |
[ |
DED81 | Cytosolic asparaginyl-tRNA synthetase, required for protein synthesis, catalyzes the specific attach... | 2 | 2 | 5.4% | 8.28E-4 | 62207 |
[ |
YOR051C | Nuclear protein that inhibits replication of Brome mosaic virus in S. cerevisiae, which is a model s... | 2 | 2 | 5.1% | 0.00111 | 47352 |
[ |
GFA1 | Glutamine-fructose-6-phosphate amidotransferase, catalyzes the formation of glucosamine-6-P and glut... | 2 | 2 | 4.7% | 6.4E-4 | 80047 |
[ |
ERG11 | Lanosterol 14-alpha-demethylase, catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimeth... | 2 | 2 | 4.7% | 8.66E-4 | 60720 |
[ |
SSE1 | ATPase that is a component of the heat shock protein Hsp90 chaperone complex; binds unfolded protein... | 2 | 2 | 4.6% | 6.62E-4 | 77367 |
[ |
SPC72 | Component of the cytoplasmic Tub4p (gamma-tubulin) complex, binds spindle pole bodies and links them... | 2 | 2 | 4.0% | 7.38E-4 | 72105 |
[ |
MES1 | Methionyl-tRNA synthetase, forms a complex with glutamyl-tRNA synthetase (Gus1p) and Arc1p, which in... | 2 | 3 | 3.9% | 9.17E-4 | 85678 |
[ |
DED1 | ATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase, required for translation initiation of all ye... | 2 | 3 | 3.8% | 0.00114 | 65553 |
[ |
MET6 | Cobalamin-independent methionine synthase, involved in methionine biosynthesis and regeneration; req... | 2 | 3 | 3.5% | 8.97E-4 | 85860 |
[ |
ADE6 | Formylglycinamidine-ribonucleotide (FGAM)-synthetase, catalyzes a step in the 'de novo' purine nucle... | 2 | 2 | 3.4% | 3.38E-4 | 148904 |
[ |
PYC2 | Pyruvate carboxylase isoform, cytoplasmic enzyme that converts pyruvate to oxaloacetate; highly simi... | 2 | 5 | 3.2% | 9.72E-4 | 130167 |
[ |
CDC60 | Cytosolic leucyl tRNA synthetase, ligates leucine to the appropriate tRNA | 2 | 2 | 2.8% | 4.21E-4 | 124141 |
[ |
TIF4631 | Translation initiation factor eIF4G, subunit of the mRNA cap-binding protein complex (eIF4F) that al... | 2 | 2 | 2.8% | 4.82E-4 | 107101 |
[ |
URA2 | Bifunctional carbamoylphosphate synthetase (CPSase)-aspartate transcarbamylase (ATCase), catalyzes t... | 3 | 4 | 2.5% | 4.15E-4 | 245123 |
[ |
GCN1 | Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn20p; proposed to stimulate ... | 2 | 2 | 1.6% | 1.72E-4 | 296697 |
[ |
UBR1 | E3 ubiquitin ligase (N-recognin), forms heterodimer with Rad6p to ubiquitinate substrates in the N-e... | 2 | 2 | 1.6% | 2.35E-4 | 224836 |
[ |
ACC1 | Acetyl-CoA carboxylase, biotin containing enzyme that catalyzes the carboxylation of acetyl-CoA to f... | 2 | 3 | 1.5% | 3.08E-4 | 250351 |