DTASelect v2.0.20 /data/1/catclw/Projects/Jamie/November/splitted /garibaldi/people-b/applications/yates/dbase/SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta SEQUEST 3.0 in SQT format. --trypstat -e con -p 1 --fp 0.1 true Use criteria 0.0 Minimum peptide confidence 0.1 Peptide false positive rate 0.0 Minimum protein confidence 1.0 Protein false positive rate 1 Minimum charge state 16 Maximum charge state 0.0 Minimum ion proportion 1000 Maximum Sp rank -1.0 Minimum Sp score Include Modified peptide inclusion Any Tryptic status requirement false Multiple, ambiguous IDs allowed Ignore Peptide validation handling XCorr Purge duplicate peptides by protein false Include only loci with unique peptide true Remove subset proteins Ignore Locus validation handling con Exclude protein names matching 0 Minimum modified peptides per locus 1000 Minimum redundancy for low coverage loci 1 Minimum peptides per locus Locus Sequence Count Spectrum Count Sequence Coverage Length MolWt pI Validation Status Descriptive Name Unique FileName XCorr DeltCN Conf% M+H+ CalcM+H+ TotalIntensity SpR ZScore IonProportion Redundancy Sequence YGR192C 14 92 53.9% 332 35747 7.0 U TDH3 SGDID:S000003424, Chr VII from 883815-882817, reverse complement, Verified ORF, "Glyceraldehyde-3-phosphate dehydrogenase, isozyme 3, involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell-wall " * Jamie_FT_01_itms_24.13896.13896.3 6.6912 0.2988 100.0 3176.9043 3173.6538 10590.0 1 6.399 36.1 3 R.IALSRPNVEVVALNDPFITNDYAAYMFK.Y * Jamie_FT_01_itms_21.12030.12030.3 7.0042 0.4371 100.0 3269.5144 3266.5913 7966.7 1 7.664 36.2 5 K.IATYQERDPANLPWGSSNVDIAIDSTGVFK.E * Jamie_FT_01_itms_24.14214.14214.3 3.7876 0.2903 100.0 2579.2444 2576.9246 4522.7 1 5.168 38.0 1 K.VINDAFGIEEGLMTTVHSLTATQK.T Jamie_FT_02_itms_5.05868.05868.2 2.4782 0.2983 100.0 1646.2322 1645.813 6901.9 1 6.015 47.1 3 R.GGRTASGNIIPSSTGAAK.A Jamie_FT_01_itms_9.05049.05049.2 3.4187 0.2676 100.0 1377.1322 1375.5217 3983.5 1 5.211 71.4 7 R.TASGNIIPSSTGAAK.A Jamie_FT_01_itms_17.09713.09713.2 4.3337 0.4371 100.0 1472.2322 1471.7344 7470.6 1 7.881 80.8 11 R.VPTVDVSVVDLTVK.L * Jamie_FT_02_itms_11.09767.09767.3 5.513 0.4058 100.0 3812.3342 3813.2097 10076.8 1 7.282 29.2 7 K.LKGVLGYTEDAVVSSDFLGDSHSSIFDASAGIQLSPK.F * Jamie_FT_03_itms_6.12048.12048.3 5.3226 0.3527 100.0 3571.8245 3571.8762 8011.8 1 8.681 29.4 37 K.GVLGYTEDAVVSSDFLGDSHSSIFDASAGIQLSPK.F Jamie_FT_01_itms_23.13715.13715.4 5.7514 0.389 100.0 3288.7366 3289.7148 9102.0 1 6.618 30.2 3 K.FVKLVSWYDNEYGYSTRVVDLVEHVAKA.- Jamie_FT_01_itms_14.08021.08021.2 3.7398 0.388 100.0 1753.0922 1753.8651 7885.6 1 7.503 69.2 9 K.LVSWYDNEYGYSTR.V Jamie_FT_01_itms_23.13613.13613.4 3.4867 0.3013 100.0 2916.2966 2915.2317 3737.6 5 5.2 25.7 2 K.LVSWYDNEYGYSTRVVDLVEHVAKA.- Jamie_FT_01_itms_23.13572.13572.3 3.549 0.297 100.0 2917.5544 2915.2317 5359.2 3 4.598 26.0 2 K.LVSWYDNEYGYSTRVVDLVEHVAKA.- Jamie_FT_01_itms_9.05269.05269.2 2.5276 0.2668 100.0 1109.3322 1109.3109 6787.1 8 4.713 66.7 1 R.VVDLVEHVAK.A Jamie_FT_01_itms_11.06585.06585.2 2.8236 0.1001 99.8 1180.9722 1180.3898 9200.6 2 4.659 75.0 1 R.VVDLVEHVAKA.- YEL026W 3 7 49.2% 126 13569 7.9 U SNU13 SGDID:S000000752, Chr V from 101943-102323, Verified ORF, "RNA binding protein, part of U3 snoRNP involved in rRNA processing, part of U4/U6-U5 tri-snRNP involved in mRNA splicing, similar to human 15.5K protein" * Jamie_FT_01_itms_33.19787.19787.2 4.106 0.3758 100.0 2568.392 2567.948 9164.4 1 6.234 41.3 4 K.AFPLADAALTQQILDVVQQAANLR.Q * Jamie_FT_01_itms_36.21599.21599.2 2.6762 0.2087 100.0 3242.5522 3241.7397 11737.5 9 4.494 22.2 1 R.GISEFIIMAADCEPIEILLHLPLLCEDK.N * Jamie_FT_01_itms_35.20987.20987.3 5.0753 0.3501 100.0 4403.7544 4401.093 7650.6 1 6.197 24.3 2 R.GISEFIIMAADCEPIEILLHLPLLCEDKNVPYVFVPSR.V YJR009C 12 57 45.5% 332 35847 7.0 U TDH2 SGDID:S000003769, Chr X from 454595-453597, reverse complement, Verified ORF, "Glyceraldehyde-3-phosphate dehydrogenase, isozyme 2, involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell-wall " * Jamie_FT_02_itms_15.11813.11813.3 7.4366 0.4271 100.0 3304.5244 3304.727 11077.8 1 8.203 36.2 14 K.IATFQERDPANLPWASLNIDIAIDSTGVFK.E Jamie_FT_01_itms_22.12995.12995.3 3.8632 0.3269 100.0 2593.9443 2594.9575 4898.4 1 5.861 29.3 3 K.VINDAFGIEEGLMTTVHSMTATQK.T Jamie_FT_02_itms_5.05868.05868.2 2.4782 0.2983 100.0 1646.2322 1645.813 6901.9 1 6.015 47.1 3 R.GGRTASGNIIPSSTGAAK.A Jamie_FT_01_itms_9.05049.05049.2 3.4187 0.2676 100.0 1377.1322 1375.5217 3983.5 1 5.211 71.4 7 R.TASGNIIPSSTGAAK.A Jamie_FT_01_itms_17.09713.09713.2 4.3337 0.4371 100.0 1472.2322 1471.7344 7470.6 1 7.881 80.8 11 R.VPTVDVSVVDLTVK.L * Jamie_FT_02_itms_13.10901.10901.3 3.1031 0.1762 97.6 3772.0745 3774.173 10802.3 17 4.275 17.4 1 K.LKGVLGYTEDAVVSSDFLGDSNSSIFDAAAGIQLSPK.F Jamie_FT_01_itms_23.13715.13715.4 5.7514 0.389 100.0 3288.7366 3289.7148 9102.0 1 6.618 30.2 3 K.FVKLVSWYDNEYGYSTRVVDLVEHVAKA.- Jamie_FT_01_itms_14.08021.08021.2 3.7398 0.388 100.0 1753.0922 1753.8651 7885.6 1 7.503 69.2 9 K.LVSWYDNEYGYSTR.V Jamie_FT_01_itms_23.13613.13613.4 3.4867 0.3013 100.0 2916.2966 2915.2317 3737.6 5 5.2 25.7 2 K.LVSWYDNEYGYSTRVVDLVEHVAKA.- Jamie_FT_01_itms_23.13572.13572.3 3.549 0.297 100.0 2917.5544 2915.2317 5359.2 3 4.598 26.0 2 K.LVSWYDNEYGYSTRVVDLVEHVAKA.- Jamie_FT_01_itms_9.05269.05269.2 2.5276 0.2668 100.0 1109.3322 1109.3109 6787.1 8 4.713 66.7 1 R.VVDLVEHVAK.A Jamie_FT_01_itms_11.06585.06585.2 2.8236 0.1001 99.8 1180.9722 1180.3898 9200.6 2 4.659 75.0 1 R.VVDLVEHVAKA.- YLR044C 18 93 44.4% 563 61495 6.2 U PDC1 SGDID:S000004034, Chr XII from 234082-232391, reverse complement, Verified ORF, "Major of three pyruvate decarboxylase isozymes, key enzyme in alcoholic fermentation, decarboxylates pyruvate to acetaldehyde; subject to glucose-, ethanol-, and autoregulation; involved in amino acid catabolism" Jamie_FT_01_itms_13.07249.07249.2 4.9722 0.4049 100.0 1999.6921 1999.1063 8881.5 1 8.279 63.9 4 R.WAGNANELNAAYAADGYAR.I * Jamie_FT_02_itms_14.11208.11208.3 3.166 0.3043 100.0 4399.6143 4400.0156 6274.7 4 4.973 18.0 1 K.GMSCIITTFGVGELSALNGIAGSYAEHVGVLHVVGVPSISAQAK.Q * Jamie_FT_02_itms_14.11213.11213.4 6.1795 0.3766 100.0 4400.4966 4400.0156 7383.5 1 5.645 20.5 2 K.GMSCIITTFGVGELSALNGIAGSYAEHVGVLHVVGVPSISAQAK.Q * Jamie_FT_01_itms_28.16706.16706.3 5.3648 0.4237 100.0 3324.4744 3325.883 9337.6 1 8.453 29.3 6 K.LLQTPIDMSLKPNDAESEKEVIDTILALVK.D * Jamie_FT_01_itms_10.05471.05471.2 3.6793 0.2242 100.0 1376.1122 1376.4846 7758.3 1 5.623 77.3 1 K.NPVILADACCSR.H Jamie_FT_01_itms_11.06246.06246.2 1.7043 0.1092 95.6 1038.9321 1039.0928 7204.7 5 3.557 75.0 1 K.GSIDEQHPR.Y * Jamie_FT_01_itms_33.19508.19508.3 5.444 0.437 100.0 4664.784 4664.2197 10998.6 1 6.577 18.0 1 R.YGGVYVGTLSKPEVKEAVESADLILSVGALLSDFNTGSFSYSYK.T * Jamie_FT_01_itms_27.16153.16153.3 4.7059 0.3992 100.0 3984.5942 3983.4614 8340.9 98 5.645 17.4 1 L.SKPEVKEAVESADLILSVGALLSDFNTGSFSYSYKTK.N * Jamie_FT_01_itms_34.20232.20232.2 5.9226 0.4492 100.0 3088.112 3085.391 7212.4 1 8.109 46.4 20 K.EAVESADLILSVGALLSDFNTGSFSYSYK.T * Jamie_FT_04_itms_14.18750.18750.3 6.9658 0.4281 100.0 3088.9443 3085.391 10626.2 1 7.674 31.2 17 K.EAVESADLILSVGALLSDFNTGSFSYSYK.T Jamie_FT_02_itms_21.15797.15797.3 6.399 0.4388 100.0 2956.7944 2956.2756 9905.7 1 7.526 31.5 3 E.AVESADLILSVGALLSDFNTGSFSYSYK.T Jamie_FT_01_itms_34.19819.19819.2 4.8933 0.3945 100.0 2959.2522 2956.2756 8921.8 1 7.973 44.4 3 E.AVESADLILSVGALLSDFNTGSFSYSYK.T * Jamie_FT_01_itms_10.05601.05601.2 2.3875 0.2084 100.0 1017.1122 1017.21075 4402.5 1 4.767 77.8 1 K.LLTTIADAAK.G * Jamie_FT_02_itms_21.15911.15911.3 4.7841 0.3621 100.0 4076.8743 4076.5083 8742.8 1 6.736 20.4 1 N.QTTFPNNTYGISQVLWGSIGFTTGATLGAAFAAEEIDPK.K Jamie_FT_04_itms_12.17724.17724.3 5.3979 0.3835 100.0 2434.9443 2434.897 8302.7 1 6.828 40.5 9 R.VILFIGDGSLQLTVQEISTMIR.W Jamie_FT_03_itms_14.16868.16868.2 5.7065 0.3945 100.0 2436.3123 2434.897 9210.5 1 8.378 59.5 13 R.VILFIGDGSLQLTVQEISTMIR.W Jamie_FT_01_itms_21.12210.12210.3 5.4661 0.2229 100.0 2271.8044 2271.6208 7909.5 1 6.506 51.4 8 R.WGLKPYLFVLNNDGYTIEK.L Jamie_FT_01_itms_21.12441.12441.2 3.1043 0.073 99.5 2272.5723 2271.6208 6399.4 1 5.391 44.4 1 R.WGLKPYLFVLNNDGYTIEK.L YOR369C 4 16 43.4% 143 15472 4.7 U RPS12 SGDID:S000005896, Chr XV from 1028621-1028190, reverse complement, Verified ORF, "Protein component of the small (40S) ribosomal subunit; has similarity to rat ribosomal protein S12" * Jamie_FT_01_itms_31.18427.18427.2 3.6882 0.2805 100.0 2474.152 2472.7954 9281.4 1 5.279 42.9 1 Q.EETVVEQTAEVTIEDALKVVLR.T * Jamie_FT_01_itms_37.21981.21981.4 5.8243 0.3013 100.0 4227.6567 4229.004 10755.2 1 5.342 23.1 3 K.ALTRGEALLVVLVSSVTEANIIKLVEGLANDPENKVPLIK.V * Jamie_FT_02_itms_19.14555.14555.3 6.5451 0.4668 100.0 1956.0243 1956.3306 9103.8 1 8.717 52.8 6 R.GEALLVVLVSSVTEANIIK.L * Jamie_FT_02_itms_19.14520.14520.2 4.9582 0.2775 100.0 1958.1721 1956.3306 10557.9 1 6.456 61.1 6 R.GEALLVVLVSSVTEANIIK.L YBR010W 4 12 37.5% 136 15356 11.4 U HHT1 SGDID:S000000214, Chr II from 256329-256739, Verified ORF, "One of two identical histone H3 proteins (see also HHT2); core histone required for chromatin assembly, involved in heterochromatin-mediated telomeric and HM silencing; regulated by acetylation, methylation, and mitotic phosphorylation" YNL031C 4 12 37.5% 136 15356 11.4 U HHT2 SGDID:S000004976, Chr XIV from 576051-575641, reverse complement, Verified ORF, "One of two identical histone H3 proteins (see also HHT1); core histone required for chromatin assembly, involved in heterochromatin-mediated telomeric and HM silencing; regulated by acetylation, methylation, and mitotic phosphorylation" Jamie_FT_01_itms_33.19731.19731.2 2.288 0.0686 96.2 2048.112 2046.2052 9297.7 6 2.909 38.9 1 R.KQLAS*KAARKSAPST*GGVK.K Jamie_FT_01_itms_36.21305.21305.3 8.2153 0.5385 100.0 3423.8342 3424.832 5052.7 1 9.02 34.7 5 R.FQSSAIGALQESVEAYLVSLFEDTNLAAIHAK.R Jamie_FT_01_itms_36.21191.21191.4 5.7839 0.432 100.0 3425.0166 3424.832 9086.5 1 7.744 28.5 2 R.FQSSAIGALQESVEAYLVSLFEDTNLAAIHAK.R Jamie_FT_01_itms_36.21300.21300.2 4.9064 0.4016 100.0 3426.2922 3424.832 5801.6 1 7.46 35.5 4 R.FQSSAIGALQESVEAYLVSLFEDTNLAAIHAK.R Reverse_YHR141C 1 1 36.8% 106 12212 10.6 U RPL42B SGDID:S000001183, Chr VIII from 382754-382751,382309-381993, reverse complement, Verified ORF, "Protein component of the large (60S) ribosomal subunit, identical to Rpl42Ap and has similarity to rat L44; required for propagation of the killer toxin-encoding M1 double-stranded RNA satellite of the L-A double-stranded RNA virus" Reverse_YNL162W 1 1 36.8% 106 12212 10.6 U RPL42A SGDID:S000005106, Chr XIV from 331324-331327,331840-332156, Verified ORF, "Protein component of the large (60S) ribosomal subunit, identical to Rpl42Bp and has similarity to rat L44 ribosomal protein" Jamie_FT_01_itms_44.26209.26209.4 2.8146 0.1722 96.2 4883.0566 4877.3726 6734.4 13 4.175 18.0 1 R.DY*RRKGQAFLSAKGAKYQT*VKHQTHKRCTKGKCYTKRTK.P YHR174W 14 40 35.0% 437 46914 6.0 U ENO2 SGDID:S000001217, Chr VIII from 451327-452640, Verified ORF, "Enolase II, a phosphopyruvate hydratase that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate during glycolysis and the reverse reaction during gluconeogenesis; expression is induced in response to glucose" Jamie_FT_01_itms_10.05688.05688.2 2.2025 0.2427 100.0 1416.5122 1417.556 6917.3 1 6.213 62.5 1 R.GNPTVEVELTTEK.G * Jamie_FT_01_itms_14.08393.08393.2 2.6966 0.2718 100.0 1415.2322 1415.694 9235.1 28 5.366 46.4 2 K.LGANAILGVSMAAAR.A Jamie_FT_01_itms_31.18236.18236.3 4.049 0.3118 100.0 3416.0942 3415.7385 8948.6 1 5.429 24.2 1 K.RYGASAGNVGDEGGVAPNIQTAEEALDLIVDAIK.A Jamie_FT_01_itms_34.20006.20006.2 4.5897 0.4794 100.0 3261.3123 3259.551 4442.3 1 7.561 32.8 9 R.YGASAGNVGDEGGVAPNIQTAEEALDLIVDAIK.A Jamie_FT_01_itms_33.19503.19503.3 5.2724 0.4358 100.0 3261.8044 3259.551 7587.9 1 7.204 25.8 3 R.YGASAGNVGDEGGVAPNIQTAEEALDLIVDAIK.A Jamie_FT_01_itms_34.19856.19856.2 4.4348 0.3759 100.0 3041.4321 3039.3232 5503.6 1 6.936 35.0 3 G.ASAGNVGDEGGVAPNIQTAEEALDLIVDAIK.A Jamie_FT_01_itms_33.19572.19572.2 3.8992 0.3731 100.0 2025.6322 2025.3068 7236.6 1 6.467 52.8 4 V.APNIQTAEEALDLIVDAIK.A Jamie_FT_01_itms_16.09205.09205.2 2.8437 0.2331 100.0 1375.6522 1374.4993 8137.7 4 5.399 63.6 5 K.IGLDCASSEFFK.D Jamie_FT_01_itms_25.14609.14609.3 5.5515 0.3729 100.0 2989.5544 2986.269 8210.0 1 6.043 34.8 2 K.RYPIVSIEDPFAEDDWEAWSHFFK.T Jamie_FT_01_itms_28.16518.16518.3 3.5173 0.2626 100.0 2834.3643 2830.0815 8832.2 24 4.553 26.1 1 R.YPIVSIEDPFAEDDWEAWSHFFK.T * Jamie_FT_01_itms_17.09753.09753.2 2.5055 0.2391 100.0 1855.6721 1856.0862 9874.4 1 4.747 55.9 4 K.TAGIQIVADDLTVTNPAR.I Jamie_FT_01_itms_10.05687.05687.2 1.7204 0.2051 99.4 814.1722 815.0006 6288.4 1 5.1 85.7 1 K.AADALLLK.V Jamie_FT_01_itms_10.05834.05834.2 2.0898 0.1083 97.7 1291.0721 1289.4717 6901.0 11 3.851 59.1 1 K.VNQIGTLSESIK.A Jamie_FT_02_itms_14.11531.11531.2 4.2409 0.2541 100.0 1823.7922 1823.0099 10777.1 1 5.596 62.5 3 R.SGETEDTFIADLVVGLR.T YBR109C 2 22 34.0% 147 16135 4.3 U CMD1 SGDID:S000000313, Chr II from 458356-457913, reverse complement, Verified ORF, "Calmodulin; Ca++ binding protein that regulates Ca++ independent processes (mitosis, bud growth, actin organization, endocytosis, etc.) and Ca++ dependent processes (stress-activated pathways), targets include Nuf1p, Myo2p and calcineurin" * Jamie_FT_01_itms_30.17825.17825.3 5.6228 0.4311 100.0 4109.2744 4109.526 9523.5 1 8.155 27.8 14 R.SLGLSPSEAEVNDLMNEIDVDGNHQIEFSEFLALMSR.Q * Jamie_FT_01_itms_16.09054.09054.2 3.9393 0.3013 100.0 1511.3922 1510.5975 12166.6 1 6.44 66.7 8 K.SNDSEQELLEAFK.V YAL038W 13 55 33.8% 500 54545 7.7 U CDC19 SGDID:S000000036, Chr I from 71787-73289, Verified ORF, "Pyruvate kinase, functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvate, the input for aerobic (TCA cycle) or anaerobic (glucose fermentation) respiration" * Jamie_FT_01_itms_13.07453.07453.2 3.1279 0.2681 100.0 1345.8722 1345.5388 8885.5 3 5.618 66.7 2 R.LTSLNVVAGSDLR.R * Jamie_FT_01_itms_11.06176.06176.2 2.0921 0.0532 94.3 1228.1921 1228.391 6159.8 141 3.252 60.0 3 K.TNNPETLVALR.K * Jamie_FT_02_itms_14.11451.11451.3 5.7547 0.4414 100.0 2267.7544 2267.581 8322.7 1 7.96 43.4 5 R.IIYVDDGVLSFQVLEVVDDK.T * Jamie_FT_02_itms_14.11538.11538.2 6.3546 0.3885 100.0 2270.5122 2267.581 8162.8 1 7.145 68.4 33 R.IIYVDDGVLSFQVLEVVDDK.T * Jamie_FT_01_itms_10.05522.05522.2 2.8536 0.1956 100.0 1002.9922 1003.14355 6106.8 3 4.325 81.2 2 R.TANDVLTIR.E * Jamie_FT_01_itms_9.04860.04860.2 2.7268 0.3106 100.0 947.71216 948.12537 7032.2 19 6.086 81.2 1 K.VTDGVMVAR.G * Jamie_FT_01_itms_34.20417.20417.2 2.37 0.1448 99.5 3516.7122 3517.9502 9994.5 6 4.766 19.4 1 K.GNYPINAVTTMAETAVIAEQAIAYLPNYDDMR.N * Jamie_FT_01_itms_11.06117.06117.2 2.379 0.3525 100.0 1316.2322 1316.5406 4632.9 1 6.443 62.5 1 K.AIIVLSTSGTTPR.L * Jamie_FT_01_itms_13.07262.07262.2 2.5846 0.1366 99.8 1502.2522 1502.7661 7110.9 11 4.222 63.6 1 K.YRPNCPIILVTR.C * Jamie_FT_01_itms_22.13049.13049.3 3.1546 0.2838 100.0 2569.5244 2570.8174 10595.4 66 5.324 28.6 3 R.GVFPFVFEKEPVSDWTDDVEAR.I * Jamie_FT_01_itms_32.18745.18745.4 4.8703 0.3104 100.0 4136.6567 4131.674 8619.8 3 5.01 20.5 1 R.GVFPFVFEKEPVSDWTDDVEARINFGIEKAKEFGIL.K * Jamie_FT_01_itms_10.05693.05693.2 2.3541 0.0076 97.2 821.1122 820.964 4849.2 1 4.437 91.7 1 R.INFGIEK.A * Jamie_FT_01_itms_9.05198.05198.2 2.4503 0.0105 93.7 1343.4321 1342.4514 5234.8 2 3.001 57.7 1 K.AGAGHSNTLQVSTV.- YPL131W 8 21 33.0% 297 33715 6.8 U RPL5 SGDID:S000006052, Chr XVI from 303120-304013, Verified ORF, "Protein component of the large (60S) ribosomal subunit with similarity to E. coli L18 and rat L5 ribosomal proteins; binds 5S rRNA and is required for 60S subunit assembly" * Jamie_FT_01_itms_33.19452.19452.4 4.0634 0.0751 96.3 5887.2163 5891.6274 9537.5 3 3.583 14.3 1 R.LVTQHKAKY*NT*PKYRLVVRFTNKDIICQIISSTITGDVVLAAAYSHELPR.Y * Jamie_FT_01_itms_33.19395.19395.4 3.9369 0.1026 97.2 5894.3364 5891.6274 9046.0 1 3.539 14.3 1 R.LVT*QHKAKY*NTPKYRLVVRFTNKDIICQIISSTITGDVVLAAAYSHELPR.Y * Jamie_FT_01_itms_30.17772.17772.3 6.4109 0.5025 100.0 3438.6243 3434.8804 10169.2 1 8.819 32.5 2 R.FTNKDIICQIISSTITGDVVLAAAYSHELPR.Y * Jamie_FT_02_itms_21.15683.15683.3 4.2721 0.3079 100.0 2943.6543 2944.3208 6383.4 1 6.381 27.9 6 K.DIICQIISSTITGDVVLAAAYSHELPR.Y * Jamie_FT_01_itms_33.19452.19452.2 4.5799 0.5085 100.0 2944.112 2944.3208 9537.5 1 9.305 40.4 1 K.DIICQIISSTITGDVVLAAAYSHELPR.Y * Jamie_FT_01_itms_23.13682.13682.3 3.7857 0.1883 99.6 2335.2544 2334.6829 8845.4 86 4.731 26.2 3 R.YGITHGLTNWAAAYATGLLIAR.R * Jamie_FT_01_itms_15.08783.08783.2 2.6624 0.0795 99.4 1062.2322 1061.2694 8472.2 2 4.331 93.8 6 K.VFLDIGLQR.T * Jamie_FT_01_itms_23.13579.13579.2 3.1291 0.0875 99.6 2095.3523 2094.285 7417.2 1 4.309 50.0 1 R.FPGWDFETEEIDPELLR.S Reverse_YGL032C 1 1 32.2% 87 9464 7.1 U AGA2 SGDID:S000003000, Chr VII from 436838-436575, reverse complement, Verified ORF, "Adhesion subunit of a-agglutinin of a-cells, C-terminal sequence acts as a ligand for alpha-agglutinin (Sag1p) during agglutination, modified with O-linked oligomannosyl chains, linked to anchorage subunit Aga1p via two disulfide bonds" * Jamie_FT_01_itms_23.13554.13554.3 2.7754 0.1287 93.1 3194.6943 3192.3025 10295.1 75 3.501 20.4 1 -.FVYQTNIPSGKSTTSPHSGCNS*VFTVS*K.Y YBL002W 3 4 32.1% 131 14237 10.1 U HTB2 SGDID:S000000098, Chr II from 236495-236890, Verified ORF, "One of two nearly identical (see HTB1) histone H2B subtypes required for chromatin assembly and chromosome function; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates transcriptional activation, meiotic DSB formation and H3 methylation" YDR224C 3 4 32.1% 131 14252 10.1 U HTB1 SGDID:S000002632, Chr IV from 914706-914311, reverse complement, Verified ORF, "One of two nearly identical (see HTB2) histone H2B subtypes required for chromatin assembly and chromosome function; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates transcriptional activation, meiotic DSB formation and H3 methylation " Jamie_FT_02_itms_5.05848.05848.2 2.2142 0.1746 99.6 1508.6522 1508.758 7627.0 1 4.074 61.5 1 R.IATEASKLAAYNKK.S Jamie_FT_01_itms_12.07094.07094.2 1.867 0.0456 92.6 955.1922 954.19794 4067.4 244 2.951 56.2 1 R.LILPGELAK.H Jamie_FT_02_itms_5.05784.05784.2 2.2296 0.2296 100.0 1980.5322 1981.1295 7628.2 1 4.611 41.7 2 K.HAVSEGTRAVTKYSSSTQA.- YBL003C 3 10 28.8% 132 13989 10.7 U HTA2 SGDID:S000000099, Chr II from 235795-235397, reverse complement, Verified ORF, "One of two nearly identical (see also HTA1) histone H2A subtypes; core histone required for chromatin assembly and chromosome function; DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p " YDR225W 3 10 28.8% 132 13989 10.7 U HTA1 SGDID:S000002633, Chr IV from 915524-915922, Verified ORF, "One of two nearly identical (see also HTA2) histone H2A subtypes; core histone required for chromatin assembly and chromosome function; DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p " Jamie_FT_01_itms_11.06479.06479.2 2.272 0.1141 99.5 918.8522 918.0837 5176.2 48 4.12 75.0 1 K.AGLTFPVGR.V Jamie_FT_01_itms_49.29285.29285.3 5.3658 0.426 100.0 2948.1843 2947.4014 6865.7 1 8.105 31.2 2 R.IGSGAPVYLTAVLEYLAAEILELAGNAAR.D Jamie_FT_01_itms_49.29184.29184.2 6.2581 0.4178 100.0 2949.112 2947.4014 9673.8 1 7.893 46.4 7 R.IGSGAPVYLTAVLEYLAAEILELAGNAAR.D YHR010W 1 1 27.2% 136 15531 10.4 U RPL27A SGDID:S000001052, Chr VIII from 126515-126545,127107-127486, Verified ORF, "Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl27Bp and has similarity to rat L27 ribosomal protein" * Jamie_FT_01_itms_26.15542.15542.3 3.1804 0.0876 90.7 4120.4346 4123.7104 10361.9 363 3.175 16.0 1 R.GRYAGKKVVIVKPHDEGSKSHPFGHALVAGIERY*PLK.V YMR116C 5 10 26.0% 319 34805 6.2 U ASC1 SGDID:S000004722, Chr XIII from 500687-500151,499877-499455, reverse complement, Verified ORF, "WD repeat protein (G-beta like protein) involved in translation regulation; required for repression of Gcn4p activity in the absence of amino-acid starvation; core component of the ribosome; ortholog of mammalian RACK1" * Jamie_FT_01_itms_12.06663.06663.2 2.8063 0.2408 100.0 1438.9321 1439.5675 6114.6 1 5.194 72.7 1 R.LWDVATGETYQR.F * Jamie_FT_01_itms_9.04896.04896.2 2.05 0.147 99.5 922.97217 922.08746 6800.0 11 4.508 75.0 1 K.ASMIISGSR.D * Jamie_FT_01_itms_13.07675.07675.2 3.9411 0.1886 100.0 1267.6921 1266.4827 8067.4 1 6.809 81.8 4 R.YWLAAATATGIK.V * Jamie_FT_01_itms_24.13928.13928.3 2.7739 0.1448 95.7 2563.8542 2560.8657 4563.5 14 3.748 29.8 1 K.VFSLDPQYLVDDLRPEFAGYSK.A * Jamie_FT_02_itms_10.08951.08951.3 5.092 0.3733 100.0 2965.0745 2962.248 8789.3 1 6.58 27.8 3 K.AAEPHAVSLAWSADGQTLFAGYTDNVIR.V YJR123W 5 10 25.3% 225 25039 8.6 U RPS5 SGDID:S000003884, Chr X from 651816-652493, Verified ORF, "Protein component of the small (40S) ribosomal subunit, the least basic of the non-acidic ribosomal proteins; phosphorylated in vivo; essential for viability; has similarity to E. coli S7 and rat S5 ribosomal proteins" * Jamie_FT_01_itms_14.08149.08149.2 2.3942 0.1574 99.8 1265.1322 1265.4087 10406.6 1 4.362 75.0 1 K.DASLVDYVQVR.Q * Jamie_FT_01_itms_34.19919.19919.2 6.0726 0.5228 100.0 3058.392 3058.462 9382.6 1 11.172 44.4 4 K.HTLDIINVLTDQNPIQVVVDAITNTGPR.E * Jamie_FT_01_itms_34.19952.19952.3 5.3403 0.3548 100.0 3062.0645 3058.462 10028.7 1 6.995 28.7 2 K.HTLDIINVLTDQNPIQVVVDAITNTGPR.E * Jamie_FT_01_itms_31.18401.18401.3 4.8314 0.3094 100.0 3661.7644 3661.0635 7793.2 1 5.934 27.3 2 K.HTLDIINVLTDQNPIQVVVDAITNTGPREDTTR.V * Jamie_FT_01_itms_17.10104.10104.2 2.6815 0.2022 100.0 1342.0322 1340.609 8485.4 1 5.223 58.3 1 R.VNQAIALLTIGAR.E YIL149C 40 103 24.9% 1679 195140 6.0 U MLP2 SGDID:S000001411, Chr IX from 68067-63028, reverse complement, Verified ORF, "Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the nuclear interior; involved in the Tel1p pathway that controls telomere length" * Jamie_FT_01_itms_13.07248.07248.2 2.6503 0.1237 100.0 1043.4122 1043.2487 4314.0 1 4.352 87.5 4 K.LNVLVDEIK.S * Jamie_FT_01_itms_9.05075.05075.2 3.7914 0.3226 100.0 1449.3922 1448.443 7129.6 1 6.529 79.2 1 R.DQGNDSLNDDLNK.E * Jamie_FT_01_itms_11.06012.06012.2 3.1539 0.1803 100.0 1819.8722 1819.8363 5330.0 5 4.235 53.3 7 R.DQGNDSLNDDLNKENK.L * Jamie_FT_01_itms_15.08743.08743.2 2.5165 0.1289 99.8 1277.1322 1277.5397 7972.5 10 4.415 72.2 2 K.LMEMENILQR.C * Jamie_FT_01_itms_10.05750.05750.2 2.4592 0.1524 100.0 973.7322 974.1454 6630.9 2 4.067 87.5 1 K.SNAISLQLK.Y * Jamie_FT_01_itms_11.06109.06109.2 1.9923 0.197 99.8 1172.0521 1172.237 6003.5 247 4.038 55.6 3 K.SSHVENLEEK.L * Jamie_FT_01_itms_15.08838.08838.2 3.2496 0.3471 100.0 2016.8922 2016.2324 7819.0 1 6.796 53.3 3 K.LYQMQSNYESVFTYNK.F * Jamie_FT_01_itms_27.15876.15876.2 3.1872 0.34 100.0 2144.5723 2145.4197 9005.7 3 5.59 43.8 3 K.FQLQNQLEDFILELEHK.T * Jamie_FT_01_itms_27.15818.15818.3 4.9579 0.3114 100.0 2145.3843 2145.4197 5108.1 1 6.453 51.6 5 K.FQLQNQLEDFILELEHK.T * Jamie_FT_01_itms_29.17298.17298.3 4.0721 0.341 100.0 3060.9243 3061.5046 5684.0 1 5.635 34.4 3 K.FQLQNQLEDFILELEHKTPELISFK.E * Jamie_FT_01_itms_29.17234.17234.2 3.2726 0.3889 100.0 3061.0122 3061.5046 8925.6 1 5.783 35.4 1 K.FQLQNQLEDFILELEHKTPELISFK.E * Jamie_FT_01_itms_29.17297.17297.4 5.9725 0.397 100.0 3061.9365 3061.5046 5672.5 1 7.398 37.5 3 K.FQLQNQLEDFILELEHKTPELISFK.E * Jamie_FT_01_itms_13.07525.07525.2 2.2062 0.0086 94.2 934.8122 935.10815 4333.8 43 3.081 85.7 1 K.TPELISFK.E * Jamie_FT_01_itms_14.08039.08039.2 3.1182 0.1893 100.0 1321.7122 1321.5107 8725.1 1 5.284 72.7 3 R.STELLETVSLTK.R * Jamie_FT_01_itms_20.11965.11965.3 3.2757 0.208 99.3 2856.5942 2855.3018 9431.7 51 3.961 23.0 3 K.LLLLNTSAIQETASPLSQDELISLRK.I * Jamie_FT_01_itms_13.07308.07308.2 3.9513 0.3005 100.0 2245.652 2246.4363 7997.7 1 7.465 52.6 2 K.ILESSNIVNENDSQAIITER.L * Jamie_FT_01_itms_13.07319.07319.3 3.5848 0.2083 100.0 2246.8743 2246.4363 8521.7 1 4.775 36.8 1 K.ILESSNIVNENDSQAIITER.L * Jamie_FT_01_itms_12.07182.07182.2 2.5876 0.1859 100.0 1549.5122 1549.721 7210.7 2 4.932 66.7 1 R.LVEFSNVNELQEK.N * Jamie_FT_01_itms_13.07431.07431.2 2.1233 0.0549 96.2 1132.7922 1131.2836 6247.0 108 3.219 68.8 1 K.NVELLNCIR.I * Jamie_FT_01_itms_14.08311.08311.2 3.8897 0.3078 100.0 1344.6522 1343.5199 7986.4 1 5.873 77.3 6 K.DAIIELENINAK.M * Jamie_FT_01_itms_10.05801.05801.2 3.2563 0.3024 100.0 1257.1322 1257.4325 7539.2 1 5.652 80.0 1 K.VENSAIIQNLR.K * Jamie_FT_01_itms_15.08526.08526.2 2.6111 0.1196 99.8 1244.1522 1244.3434 7416.7 388 4.457 61.1 4 K.TTLEDFENFK.G * Jamie_FT_01_itms_10.05423.05423.2 2.0583 0.1122 98.7 995.2922 994.1326 5410.4 153 3.632 62.5 1 K.ETASFIPTK.E * Jamie_FT_01_itms_9.05261.05261.2 2.5677 0.0175 97.8 987.6322 988.1289 3817.5 78 3.192 78.6 1 K.IQEIESIR.S * Jamie_FT_01_itms_10.05532.05532.2 4.1666 0.4015 100.0 1453.6322 1452.5787 6856.4 1 7.583 86.4 1 K.WAQNTIDDTEMK.M * Jamie_FT_01_itms_13.07574.07574.2 2.263 0.1649 100.0 1005.15216 1005.15643 5658.3 4 4.295 87.5 2 K.SLLTELSNK.E * Jamie_FT_01_itms_13.07327.07327.2 2.3318 0.1753 99.6 1808.7322 1807.9548 7348.1 1 4.161 53.3 2 K.FLDQNSDASTLEPTLR.K * Jamie_FT_01_itms_11.06510.06510.2 2.7223 0.0489 99.0 1228.6122 1228.4313 6033.1 218 3.922 66.7 1 K.ELEQIQVQLK.D * Jamie_FT_01_itms_10.05753.05753.2 1.9927 0.1066 97.8 1079.7522 1080.2941 6928.6 3 4.297 75.0 1 K.MSTIVQLCK.K * Jamie_FT_01_itms_14.07981.07981.2 1.9858 0.0711 96.8 944.5722 945.1039 5895.6 43 3.898 71.4 1 K.DLSELVIR.L * Jamie_FT_01_itms_9.05078.05078.2 1.8038 0.1061 96.4 1029.6522 1031.1943 7432.7 40 3.695 68.8 1 R.VENSVLIEK.V * Jamie_FT_01_itms_15.08771.08771.2 3.0266 0.2563 100.0 1049.0922 1049.2584 4859.8 1 5.016 93.8 9 K.LVSLFSNLR.H * Jamie_FT_01_itms_20.11910.11910.3 4.5233 0.2122 100.0 2847.3542 2848.1426 11563.1 2 4.507 30.4 3 R.TQTLSEKEYQCSAVIIDEFKDITK.E * Jamie_FT_01_itms_3.01308.01308.2 2.0827 0.2417 100.0 1138.6322 1138.2224 7669.4 200 4.596 68.8 1 R.YQDLSQQQK.D * Jamie_FT_01_itms_9.05346.05346.2 2.9037 0.0788 99.8 1033.2322 1033.1669 5230.8 5 3.583 81.2 1 K.LTNEISDLK.G * Jamie_FT_01_itms_8.04807.04807.2 4.0775 0.3595 100.0 1475.9722 1476.54 8432.9 1 7.617 73.1 1 K.LSSAENANADLENK.F * Jamie_FT_01_itms_13.07461.07461.2 2.4846 0.2734 100.0 1472.2322 1472.6384 5122.8 37 4.979 45.8 1 K.QQAALTNELNELK.A * Jamie_FT_01_itms_3.01386.01386.2 1.9202 0.1739 99.5 1157.1122 1157.1791 5742.3 77 4.584 50.0 1 K.TANSSSDAFEK.L * Jamie_FT_04_itms_2.08070.08070.2 3.1682 0.0455 99.5 1212.2122 1212.4319 5593.3 112 4.158 65.0 6 R.TLNIQPEVAVK.A * Jamie_FT_01_itms_14.08208.08208.2 3.5134 0.3948 100.0 2054.412 2054.1777 9222.1 1 7.603 44.7 10 K.AAINFSNVTDLTNNSTDGAK.I YOL039W 1 1 23.6% 106 10746 4.0 U RPP2A SGDID:S000005399, Chr XV from 254295-254615, Verified ORF, "Ribosomal protein P2 alpha, a component of the ribosomal stalk, which is involved in the interaction between translational elongation factors and the ribosome; regulates the accumulation of P1 (Rpp1Ap and Rpp1Bp) in the cytoplasm" * Jamie_FT_02_itms_14.11390.11390.3 4.1441 0.4009 100.0 2617.0144 2616.9658 8512.2 1 6.983 31.2 1 K.AILESVGIEIEDEKVSSVLSALEGK.S YDR385W 11 16 23.5% 842 93289 6.3 U EFT2 SGDID:S000002793, Chr IV from 1243220-1245748, Verified ORF, "Elongation factor 2 (EF-2), also encoded by EFT1; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin" YOR133W 11 16 23.5% 842 93289 6.3 U EFT1 SGDID:S000005659, Chr XV from 575098-577626, Verified ORF, "Elongation factor 2 (EF-2), also encoded by EFT2; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin" Jamie_FT_02_itms_15.11801.11801.3 3.6092 0.1717 99.0 2975.8145 2977.2573 8857.3 1 4.607 26.9 2 K.TDGNSFLINLIDSPGHVDFSSEVTAALR.V Jamie_FT_02_itms_16.12713.12713.3 4.1018 0.1283 98.4 2574.5942 2574.8975 9697.9 3 4.685 28.3 1 R.VTDGALVVVDTIEGVCVQTETVLR.Q Jamie_FT_01_itms_19.11071.11071.3 3.8764 0.2141 100.0 2126.7844 2125.4294 7616.4 1 4.72 39.7 1 R.ALLELQVSKEDLYQTFAR.T Jamie_FT_01_itms_18.10777.10777.2 2.171 0.0812 97.5 1211.7522 1211.3623 6111.3 7 3.532 77.8 1 R.LWGDSFFNPK.T Jamie_FT_01_itms_30.17591.17591.3 3.4435 0.1867 99.3 2970.2344 2966.555 6428.1 4 4.961 29.8 1 R.KFLPAADALLEMIVLHLPSPVTAQAYR.A Jamie_FT_01_itms_17.09690.09690.2 3.2114 0.3211 100.0 1488.6322 1488.7673 8962.5 1 6.081 65.4 5 K.FSVSPVVQVAVEVK.N Jamie_FT_01_itms_19.11181.11181.2 2.2774 0.1027 97.8 2133.892 2132.3464 11139.0 91 3.773 36.1 1 K.IWCFGPDGNGPNLVIDQTK.A Jamie_FT_01_itms_23.13779.13779.3 3.2935 0.1117 96.3 2407.9443 2405.7588 7724.2 2 4.276 30.0 1 K.AVQYLHEIKDSVVAAFQWATK.E Jamie_FT_01_itms_15.08697.08697.2 2.6181 0.2384 100.0 1268.7322 1267.4674 9823.5 1 5.419 68.2 1 R.ATYAGFLLADPK.I Jamie_FT_01_itms_18.10809.10809.2 2.0093 0.1136 97.3 1800.2922 1801.0087 7663.8 1 4.618 46.7 1 K.AYLPVNESFGFTGELR.Q Jamie_FT_01_itms_9.05274.05274.2 2.8222 0.0203 98.9 900.53217 900.0659 5970.6 4 4.893 81.2 1 K.AGEIVLAAR.K YNL178W 5 12 22.1% 240 26503 9.4 U RPS3 SGDID:S000005122, Chr XIV from 302682-303404, Verified ORF, "Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; has similarity to E. coli S3 and rat S3 ribosomal proteins" * Jamie_FT_02_itms_14.11143.11143.3 3.4551 0.1813 99.7 2119.2844 2120.4124 9963.6 2 4.645 35.3 1 R.KLVADGVFYAELNEFFTR.E * Jamie_FT_02_itms_16.12794.12794.2 4.6004 0.4195 100.0 1991.8322 1992.2383 9753.5 1 7.507 56.2 5 K.LVADGVFYAELNEFFTR.E * Jamie_FT_01_itms_10.05562.05562.2 4.1329 0.3576 100.0 1439.5521 1438.5339 7983.8 1 8.164 83.3 1 R.ELAEEGYSGVEVR.V * Jamie_FT_01_itms_14.07890.07890.2 2.7313 0.1664 100.0 1170.7322 1171.4227 8243.4 24 4.788 72.2 3 R.INELTLLVQK.R * Jamie_FT_01_itms_13.07347.07347.2 2.5744 0.2473 100.0 1353.5122 1353.5608 3723.8 1 4.339 72.7 2 K.YAPGTIVLYAER.V Reverse_YNL024C 1 1 22.0% 246 27738 6.2 U YNL024C SGDID:S000004969, Chr XIV from 587848-587108, reverse complement, Uncharacterized ORF, "Putative S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family" * Jamie_FT_02_itms_17.13475.13475.4 3.2585 0.0927 93.5 6027.177 6022.707 8824.1 14 3.195 12.9 1 K.DIDTVYVKT*GDHFTNKELLGVCLGVLGTGSGLELVKKFQKTGNVT*KSLLHDVSK.E YGL123W 3 6 22.0% 254 27450 10.4 U RPS2 SGDID:S000003091, Chr VII from 277623-278387, Verified ORF, "Protein component of the small (40S) subunit, essential for control of translational accuracy; has similarity to E. coli S5 and rat S2 ribosomal proteins" * Jamie_FT_01_itms_23.13760.13760.2 4.0826 0.293 100.0 1741.1522 1741.0807 10827.8 1 6.901 64.3 4 K.ITTIEEIFLHSLPVK.E * Jamie_FT_01_itms_33.19740.19740.4 4.0559 0.2635 100.0 4491.8564 4492.3145 11533.0 2 4.183 18.4 1 K.ITTIEEIFLHSLPVKEFQIIDTLLPGLQDEVMNIKPVQK.Q * Jamie_FT_01_itms_16.09122.09122.2 2.1772 0.1981 99.8 1836.8522 1836.052 8716.9 41 3.663 37.5 1 K.LLQLAGVEDVYTQSNGK.T YOL086C 5 13 21.0% 348 36849 6.7 U ADH1 SGDID:S000005446, Chr XV from 160593-159547, reverse complement, Verified ORF, "Alcohol dehydrogenase, fermentative isozyme active as homo- or heterotetramers; required for the reduction of acetaldehyde to ethanol, the last step in the glycolytic pathway" * Jamie_FT_01_itms_11.06241.06241.2 2.7268 0.0932 99.8 1015.5722 1014.21014 6956.8 2 3.384 87.5 1 K.ANELLINVK.Y * Jamie_FT_02_itms_10.09071.09071.3 3.3995 0.1905 99.3 2702.0044 2702.1033 8338.4 3 4.472 23.1 1 K.SANLMAGHWVAISGAAGGLGSLAVQYAK.A * Jamie_FT_01_itms_13.07520.07520.2 1.7294 0.1531 97.3 1620.5521 1619.815 7570.7 108 4.128 42.9 1 R.VLGIDGGEGKEELFR.S Jamie_FT_01_itms_14.08096.08096.2 2.1289 0.0838 97.9 969.09216 969.08496 6339.6 37 3.842 78.6 4 R.EALDFFAR.G * Jamie_FT_01_itms_16.09020.09020.2 3.2217 0.2695 100.0 1448.4321 1448.6996 6247.4 1 6.339 66.7 6 K.VVGLSTLPEIYEK.M Reverse_YGR016W 1 1 20.5% 190 22226 8.8 U YGR016W SGDID:S000003248, Chr VII from 522265-522837, Uncharacterized ORF, "Hypothetical protein" * Jamie_FT_02_itms_19.14162.14162.4 3.0232 0.1024 91.4 4710.2964 4711.8755 10368.6 299 3.655 15.8 1 K.QILEEQEETDLPLIS*VDEQDSEGDHTY*DSSLSLIKRNFR.R YKL060C 5 14 20.3% 359 39621 5.8 U FBA1 SGDID:S000001543, Chr XI from 327131-326052, reverse complement, Verified ORF, "Fructose 1,6-bisphosphate aldolase, a cytosolic enzyme required for glycolysis and gluconeogenesis; catalyzes the conversion of fructose 1,6 bisphosphate into two 3-carbon products: glyceraldehyde-3-phosphate and dihydroxyacetone phosphate" * Jamie_FT_01_itms_18.10231.10231.2 1.9332 0.0755 93.6 1863.6322 1864.1082 11683.8 39 3.899 37.5 3 K.TGVIVGEDVHNLFTYAK.E * Jamie_FT_01_itms_22.12812.12812.3 2.9616 0.1894 98.2 2160.7444 2161.4631 8177.2 1 4.182 31.0 1 K.FAIPAINVTSSSTAVAALEAAR.D * Jamie_FT_01_itms_21.12491.12491.2 5.7183 0.533 100.0 2163.392 2161.4631 7010.3 1 9.278 64.3 6 K.FAIPAINVTSSSTAVAALEAAR.D * Jamie_FT_01_itms_14.07989.07989.2 2.1925 0.2051 99.8 1797.1122 1796.033 9222.2 2 5.226 47.1 1 K.SPIILQTSNGGAAYFAGK.G * Jamie_FT_01_itms_16.09177.09177.2 3.0246 0.2568 100.0 1902.3922 1903.065 10874.7 4 5.698 46.7 3 K.VNLDTDCQYAYLTGIR.D YBR118W 12 35 20.1% 458 50033 9.0 U TEF2 SGDID:S000000322, Chr II from 477665-479041, Verified ORF, "Translational elongation factor EF-1 alpha; also encoded by TEF1; functions in the binding reaction of aminoacyl-tRNA (AA-tRNA) to ribosomes" YPR080W 12 35 20.1% 458 50033 9.0 U TEF1 SGDID:S000006284, Chr XVI from 700592-701968, Verified ORF, "Translational elongation factor EF-1 alpha; also encoded by TEF2; functions in the binding reaction of aminoacyl-tRNA (AA-tRNA) to ribosomes" Jamie_FT_01_itms_13.07428.07428.2 2.0479 0.1613 99.8 895.3122 895.04584 5261.0 2 4.12 100.0 2 K.YAWVLDK.L Jamie_FT_01_itms_12.06931.06931.2 2.4495 0.263 100.0 1357.5922 1356.524 7941.8 1 5.131 63.6 1 K.YQVTVIDAPGHR.D Jamie_FT_02_itms_19.14157.14157.3 5.8551 0.3397 100.0 2923.8542 2925.2898 11900.6 1 6.986 28.6 10 K.NMITGTSQADCAILIIAGGVGEFEAGISK.D Jamie_FT_01_itms_30.17907.17907.2 4.1638 0.2662 100.0 2927.2922 2925.2898 9736.4 1 6.101 33.9 2 K.NMITGTSQADCAILIIAGGVGEFEAGISK.D Jamie_FT_02_itms_22.16263.16263.3 4.1062 0.1925 100.0 3005.4543 3005.2898 10438.5 2 4.241 25.9 1 K.NMITGTS*QADCAILIIAGGVGEFEAGISK.D Jamie_FT_01_itms_34.20396.20396.2 2.7335 0.0569 97.8 3006.3123 3005.2898 10560.3 170 3.217 21.4 2 K.NMITGTS*QADCAILIIAGGVGEFEAGISK.D Jamie_FT_02_itms_22.16064.16064.3 3.9336 0.1553 99.3 3006.3843 3005.2898 11014.9 1 3.977 33.9 2 K.NMITGT*SQADCAILIIAGGVGEFEAGISK.D Jamie_FT_02_itms_22.16056.16056.3 4.7443 0.1541 100.0 3006.9243 3005.2898 7749.7 1 5.117 35.7 2 K.NMIT*GTSQADCAILIIAGGVGEFEAGISK.D Jamie_FT_01_itms_34.20214.20214.2 2.4123 0.032 94.3 3007.112 3005.2898 9738.4 45 3.003 23.2 1 K.NMIT*GTSQADCAILIIAGGVGEFEAGISK.D Jamie_FT_01_itms_18.10368.10368.2 4.0662 0.3675 100.0 1328.3522 1327.5693 8747.4 1 6.735 81.8 9 R.EHALLAFTLGVR.Q Jamie_FT_01_itms_19.10939.10939.3 3.3234 0.215 99.6 2365.4343 2364.7043 6701.8 1 4.715 35.0 1 K.TLLEAIDAIEQPSRPTDKPLR.L Jamie_FT_01_itms_10.05531.05531.2 3.2029 0.2373 100.0 1025.9922 1026.2241 4845.6 11 5.266 75.0 2 K.IGGIGTVPVGR.V YGL076C 5 17 19.7% 244 27638 10.1 U RPL7A SGDID:S000003044, Chr VII from 365999-365989,365529-365436,364967-364338, reverse complement, Verified ORF, "Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl7Bp and has similarity to E. coli L30 and rat L7 ribosomal proteins; contains a conserved C-terminal Nucleic acid Binding Domain (NDB2)" YPL198W 5 17 19.7% 244 27696 10.1 U RPL7B SGDID:S000006119, Chr XVI from 173151-173161,173571-173664,174072-174701, Verified ORF, "Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl7Ap and has similarity to E. coli L30 and rat L7 ribosomal proteins; contains a conserved C-terminal Nucleic acid Binding Domain (NDB2)" Jamie_FT_01_itms_18.10262.10262.2 2.6317 0.1304 99.4 1993.6921 1993.2665 7940.3 1 4.936 40.6 4 K.LIEPYVAYGYPSYSTIR.Q Jamie_FT_01_itms_30.17777.17777.3 5.0231 0.3568 100.0 2381.8442 2381.777 4608.6 1 7.655 40.0 4 K.YGILSIDDLIHEIITVGPHFK.Q Jamie_FT_01_itms_30.17513.17513.4 4.4975 0.2813 100.0 2381.8564 2381.777 5247.1 1 5.983 39.2 4 K.YGILSIDDLIHEIITVGPHFK.Q Jamie_FT_01_itms_30.17687.17687.2 5.3122 0.4241 100.0 2382.0322 2381.777 9624.7 1 7.694 60.0 4 K.YGILSIDDLIHEIITVGPHFK.Q Jamie_FT_01_itms_20.11983.11983.2 1.7418 0.1123 95.8 1264.4122 1265.4569 5903.1 21 4.713 72.2 1 K.QANNFLWPFK.L YOR096W 1 1 19.5% 190 21622 9.8 U RPS7A SGDID:S000005622, Chr XV from 505794-505937,506339-506767, Verified ORF, "Protein component of the small (40S) ribosomal subunit, nearly identical to Rps7Bp; interacts with Kti11p; deletion causes hypersensitivity to zymocin; has similarity to rat S7 and Xenopus S8 ribosomal proteins" * Jamie_FT_01_itms_29.17371.17371.4 3.6349 0.127 97.1 4170.3364 4168.7773 5209.0 412 3.822 18.1 1 K.ILSQAPTELELQVAQAFVELENSSPELKAELRPLQFK.S YBR009C 2 4 19.4% 103 11368 11.4 U HHF1 SGDID:S000000213, Chr II from 255682-255371, reverse complement, Verified ORF, "One of two identical histone H4 proteins (see also HHF2); core histone required for chromatin assembly and chromosome function; contributes to telomeric silencing; N-terminal domain involved in maintaining genomic integrity" YNL030W 2 4 19.4% 103 11368 11.4 U HHF2 SGDID:S000004975, Chr XIV from 576728-577039, Verified ORF, "One of two identical histone H4 proteins (see also HHF1); core histone required for chromatin assembly and chromosome function; contributes to telomeric silencing; N-terminal domain involved in maintaining genomic integrity" Jamie_FT_01_itms_14.07847.07847.2 1.7092 0.103 95.7 951.4322 951.1106 3515.0 23 3.29 71.4 1 K.SFLESVIR.D Jamie_FT_01_itms_15.08682.08682.2 2.6962 0.2959 100.0 1310.4722 1309.5455 9577.0 1 5.662 59.1 3 K.TVTSLDVVYALK.R YNL064C 2 4 19.3% 409 44671 6.3 U YDJ1 SGDID:S000005008, Chr XIV from 507098-505869, reverse complement, Verified ORF, "Protein chaperone involved in regulation of the HSP90 and HSP70 functions; involved in protein translocation across membranes; member of the DnaJ family" * Jamie_FT_01_itms_30.17737.17737.3 4.0018 0.4199 100.0 4462.9443 4461.7227 6045.4 1 6.52 18.8 2 R.DIYDQFGEDGLSGAGGAGGFPGGGFGFGDDIFSQFFGAGGAQRPR.G * Jamie_FT_01_itms_42.25050.25050.3 6.009 0.4231 100.0 3717.7444 3720.0837 8852.3 1 7.977 30.3 2 K.RDGDDLVYEAEIDLLTAIAGGEFALEHVSGDWLK.V Reverse_YJR063W 1 1 19.2% 125 13661 7.9 U RPA12 SGDID:S000003824, Chr X from 555109-555486, Verified ORF, "RNA polymerase I subunit A12.2; contains two zinc binding domains, and the N terminal domain is responsible for anchoring to the RNA pol I complex" * Jamie_FT_02_itms_20.15173.15173.2 1.5862 0.1055 91.1 2742.4321 2744.889 7431.7 92 3.28 23.9 1 K.ARLS*S*PFADDATTTVVKLNSFQSK.P YCR012W 6 11 19.0% 416 44738 7.6 U PGK1 SGDID:S000000605, Chr III from 137743-138993, Verified ORF, "3-phosphoglycerate kinase, catalyzes transfer of high-energy phosphoryl groups from the acyl phosphate of 1,3-bisphosphoglycerate to ADP to produce ATP; key enzyme in glycolysis and gluconeogenesis" * Jamie_FT_01_itms_18.10229.10229.2 2.7907 0.3402 100.0 1439.2522 1440.683 6671.9 4 6.274 53.8 4 K.ASAPGSVILLENLR.Y * Jamie_FT_01_itms_19.11291.11291.2 2.3345 0.0174 92.6 1768.5922 1769.097 9459.9 96 2.695 40.6 1 K.ALENPTRPFLAILGGAK.V * Jamie_FT_01_itms_19.11261.11261.3 3.3123 0.3014 100.0 1769.2743 1769.097 6765.6 2 5.036 42.2 1 K.ALENPTRPFLAILGGAK.V * Jamie_FT_02_itms_23.16577.16577.3 3.6814 0.2748 100.0 2722.4043 2723.2434 6156.3 84 5.201 24.0 2 K.IQLIDNLLDKVDSIIIGGGMAFTFK.K * Jamie_FT_01_itms_35.20991.20991.2 4.8404 0.2975 100.0 2724.4922 2723.2434 8787.7 1 6.79 43.8 2 K.IQLIDNLLDKVDSIIIGGGMAFTFK.K * Jamie_FT_03_itms_12.16105.16105.2 3.1392 0.3386 100.0 2393.172 2392.7112 7970.0 1 6.262 40.9 1 K.GVEVVLPVDFIIADAFSADANTK.T YOL040C 2 3 19.0% 142 16002 10.7 U RPS15 SGDID:S000005400, Chr XV from 253575-253147, reverse complement, Verified ORF, "Protein component of the small (40S) ribosomal subunit; has similarity to E. coli S19 and rat S15 ribosomal proteins" * Jamie_FT_01_itms_22.13218.13218.3 5.3827 0.4805 100.0 3167.4543 3169.625 8756.8 1 7.715 29.8 2 K.AFNQVEIRPEMLGHYLGEFSITYTPVR.H * Jamie_FT_01_itms_22.13218.13218.2 2.2108 0.3154 96.4 2111.9722 2114.4236 8756.8 7 4.97 35.3 1 P.EMLGHYLGEFSITYTPVR.H Reverse_YIR016W 1 1 18.9% 265 28882 5.3 U YIR016W SGDID:S000001455, Chr IX from 382625-383422, Uncharacterized ORF, "Hypothetical protein" * Jamie_FT_01_itms_77.46075.46075.4 2.3549 0.2409 97.4 5345.1367 5339.6406 7530.3 411 4.253 11.2 1 R.SADEVLSPAHIAGAGDRSLTMYKHFAAS*DDDVDDHPGLLS*PLLGDISSGR.S YOL012C 1 2 18.7% 134 14283 10.7 U HTZ1 SGDID:S000005372, Chr XV from 303983-303579, reverse complement, Verified ORF, "Histone variant H2AZ, exchanged for histone H2A in nucleosomes by the SWR1 complex; involved in transcriptional regulation through prevention of the spread of silent heterochromatin" * Jamie_FT_01_itms_46.27297.27297.3 7.0915 0.4719 100.0 2610.1143 2609.035 9750.0 1 9.577 42.7 2 K.AAIYLTAVLEYLTAEVLELAGNAAK.D YLR075W 3 13 18.6% 221 25361 10.0 U RPL10 SGDID:S000004065, Chr XII from 282928-283593, Verified ORF, "Protein component of the large (60S) ribosomal subunit, responsible for joining the 40S and 60S subunits; regulates translation initiation; has similarity to rat L10 ribosomal protein and to members of the QM gene family" * Jamie_FT_01_itms_26.15573.15573.3 4.764 0.2535 100.0 3360.3542 3357.7083 9032.9 1 5.388 25.9 2 K.KATVDEFPLCVHLVSNELEQLSSEALEAAR.I * Jamie_FT_01_itms_30.17892.17892.3 5.9958 0.3862 100.0 3230.5444 3229.5342 10520.3 1 7.512 28.6 3 K.ATVDEFPLCVHLVSNELEQLSSEALEAAR.I * Jamie_FT_01_itms_21.12113.12113.2 3.464 0.32 100.0 1248.3522 1247.4801 8328.2 1 5.913 80.0 8 R.VDIGQIIFSVR.T YPL124W 4 14 18.2% 253 29280 9.5 U SPC29 SGDID:S000006045, Chr XVI from 316754-317515, Verified ORF, "Inner plaque spindle pole body (SPB) component, links the central plaque component Spc42p to the inner plaque component Spc110p; required for SPB duplication" * Jamie_FT_01_itms_9.04850.04850.2 1.9528 0.1218 98.7 1088.6322 1089.0636 6343.7 276 3.83 71.4 2 K.FQDDT*LNR.V * Jamie_FT_01_itms_9.05183.05183.2 3.0979 0.247 100.0 1292.9922 1293.3788 7867.2 11 5.784 70.0 1 K.FASQNVIDDQR.L * Jamie_FT_01_itms_13.07741.07741.2 2.7998 0.3079 100.0 1694.3121 1694.8821 6527.7 3 6.237 60.7 1 R.IVYDQGTVIDNLTSR.I * Jamie_FT_01_itms_16.09217.09217.2 3.3875 0.2928 100.0 1395.9722 1394.5675 8538.7 1 6.394 77.3 10 R.LESFILNSISDR.G YDR099W 2 3 17.9% 273 31061 4.9 U BMH2 SGDID:S000002506, Chr IV from 653602-654423, Verified ORF, "14-3-3 protein, minor isoform; binds proteins and DNA, involved in regulation of many processes including exocytosis and vesicle transport, Ras/MAPK signaling during pseudohyphal development, rapamycin-sensitive signaling, and others" YER177W 2 3 18.4% 267 30091 4.9 U BMH1 SGDID:S000000979, Chr V from 545606-546409, Verified ORF, "14-3-3 protein, major isoform; binds proteins and DNA, involved in regulation of many processes including exocytosis and vesicle transport, Ras/MAPK signaling during pseudohyphal development, rapamycin-sensitive signaling, and others" Jamie_FT_02_itms_15.12180.12180.2 2.9292 0.2137 100.0 2319.3123 2319.619 10688.1 1 5.421 44.7 2 R.LGLALNFSVFYYEIQNSPDK.A Jamie_FT_01_itms_36.21347.21347.3 4.4778 0.3807 100.0 3317.8145 3317.6895 8547.6 1 6.905 26.8 1 K.QAFDDAIAELDTLSEESYKDSTLIMQLLR.D YDR050C 2 3 17.7% 248 26795 6.0 U TPI1 SGDID:S000002457, Chr IV from 556469-555723, reverse complement, Verified ORF, "Triose phosphate isomerase, abundant glycolytic enzyme; mRNA half-life is regulated by iron availability; transcription is controlled by activators Reb1p, Gcr1p, and Rap1p through binding sites in the 5' non-coding region" * Jamie_FT_01_itms_32.18930.18930.3 4.5115 0.3387 100.0 4795.1045 4792.3594 9489.6 1 5.571 19.2 2 R.QLNAVLEEVKDWTNVVVAYEPVWAIGTGLAATPEDAQDIHASIR.K * Jamie_FT_01_itms_32.18967.18967.2 4.2769 0.4314 100.0 2491.6921 2490.7783 10227.7 1 6.498 43.5 1 E.PVWAIGTGLAATPEDAQDIHASIR.K YKL042W 4 21 17.4% 363 42271 7.9 U SPC42 SGDID:S000001525, Chr XI from 358119-359210, Verified ORF, "Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate attachment of the SPB to the nuclear membrane" * Jamie_FT_01_itms_3.01305.01305.2 2.218 0.1719 100.0 926.77216 925.93036 5745.8 4 4.694 71.4 12 R.DYNDVGSR.I * Jamie_FT_01_itms_15.09003.09003.2 3.185 0.3926 100.0 1298.1921 1297.5554 8704.3 1 6.862 70.0 7 R.TLSVLTNYVMR.S * Jamie_FT_01_itms_22.12683.12683.2 2.185 0.0566 94.2 1877.4922 1878.2402 8713.2 50 3.42 40.6 1 R.MSPLPSPLNTILPINNR.L * Jamie_FT_01_itms_23.13368.13368.3 3.5853 0.2606 100.0 3064.3442 3064.4097 10769.4 117 4.855 22.1 1 K.LSLNNQLQELQSMMDGDDNIKLDNVSK.H YIL002W-A 1 1 17.4% 69 7729 4.7 U YIL002W-A SGDID:S000028835, Chr IX from 350298-350507, Uncharacterized ORF, "Identified by expression profiling and mass spectrometry" * Jamie_FT_01_itms_5.02501.02501.2 1.4623 0.1268 92.3 1190.4321 1191.237 5410.4 97 3.386 50.0 1 R.DTPEDVSTAGAK.D Reverse_YER056C-A 1 1 16.5% 121 13639 10.8 U RPL34A SGDID:S000002135, Chr V from 270183-270147,269749-269421, reverse complement, Verified ORF, "Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl34Bp and has similarity to rat L34 ribosomal protein" * Jamie_FT_02_itms_14.11421.11421.2 1.6059 0.2183 98.8 2369.2722 2367.752 11315.8 230 4.252 26.3 1 -.KKAKKESKKAAET*QEKVVKK.V YLR340W 3 5 16.3% 312 33717 4.8 U RPP0 SGDID:S000004332, Chr XII from 805887-806825, Verified ORF, "Conserved ribosomal protein P0 similar to rat P0, human P0, and E. coli L10e; shown to be phosphorylated on serine 302" * Jamie_FT_01_itms_19.11336.11336.2 2.6066 0.0152 96.8 1269.1921 1268.4088 8804.7 36 3.393 70.0 1 R.GFLSDLPDFEK.L * Jamie_FT_01_itms_17.09817.09817.2 2.0629 0.1925 99.6 1296.4922 1296.5089 8739.1 11 4.012 59.1 3 K.TSFFQALGVPTK.I * Jamie_FT_01_itms_29.17351.17351.3 6.0956 0.4144 100.0 3186.3542 3185.5579 8151.9 1 8.398 34.3 1 K.DLLAVAIAASYHYPEIEDLVDRIENPEK.Y YNL225C 11 40 16.2% 581 67400 6.0 U CNM67 SGDID:S000005169, Chr XIV from 224470-222725, reverse complement, Verified ORF, "Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclear migration" * Jamie_FT_01_itms_13.07799.07799.2 3.2179 0.1925 100.0 1309.4722 1308.5786 9077.6 1 5.075 70.0 7 R.MLENVILGYQK.K * Jamie_FT_01_itms_15.08671.08671.2 2.0175 0.0735 95.5 1327.3522 1327.5236 5076.9 33 3.723 54.5 1 K.HTALLSLTDSLR.K * Jamie_FT_01_itms_31.18517.18517.2 4.711 0.5092 100.0 2906.8523 2906.2585 9751.3 1 9.044 43.8 3 R.KAELFEIPIGILFFDLYDSEENSSK.L * Jamie_FT_01_itms_31.18143.18143.3 5.9402 0.4161 100.0 2907.5645 2906.2585 10859.1 1 8.264 34.4 6 R.KAELFEIPIGILFFDLYDSEENSSK.L * Jamie_FT_01_itms_29.17315.17315.3 6.3317 0.4533 100.0 3881.7844 3883.387 5760.2 1 7.494 27.3 3 R.KAELFEIPIGILFFDLYDSEENSSKLDHILQEK.Y * Jamie_FT_01_itms_29.17267.17267.4 6.2084 0.4663 100.0 3883.2566 3883.387 6998.6 1 7.934 26.6 4 R.KAELFEIPIGILFFDLYDSEENSSKLDHILQEK.Y * Jamie_FT_01_itms_36.21171.21171.2 5.2041 0.3504 100.0 2780.2722 2778.0845 9673.1 1 6.902 50.0 5 K.AELFEIPIGILFFDLYDSEENSSK.L * Jamie_FT_01_itms_29.17256.17256.2 3.2582 0.2354 95.0 2037.4521 2036.1595 8486.7 2 3.799 46.9 1 L.YDSEENSSKLDHILQEK.Y * Jamie_FT_01_itms_10.05924.05924.2 3.8158 0.3292 100.0 1525.0721 1525.6245 7824.6 1 6.277 66.7 1 K.GFLCASQQEELSR.I * Jamie_FT_01_itms_18.10381.10381.2 3.3907 0.3212 100.0 1299.7922 1299.5963 9684.7 1 6.201 81.8 8 K.EIVLAVGIPYPK.L * Jamie_FT_01_itms_16.09297.09297.2 1.9169 0.0732 93.8 1645.6522 1645.7833 8482.5 1 3.438 66.7 1 K.FTDQAYYDFMSTR.R YBR196C 2 4 15.7% 554 61299 6.4 U PGI1 SGDID:S000000400, Chr II from 613895-612231, reverse complement, Verified ORF, "Glycolytic enzyme phosphoglucose isomerase, catalyzes the interconversion of glucose-6-phosphate and fructose-6-phosphate; required for cell cycle progression and completion of the gluconeogenic events of sporulation" * Jamie_FT_01_itms_36.21197.21197.4 5.1555 0.1477 100.0 5532.177 5529.149 6627.6 1 4.393 17.7 2 K.GAEAVDNHFTQTPLEDNIPLLGGLLSVWYNNFFGAQTHLVAPFDQYLHR.F * Jamie_FT_02_itms_22.16338.16338.4 5.3434 0.0207 96.7 4229.4565 4227.7627 11667.4 40 4.547 16.7 2 K.ITPATLGALIAYYEHVTFTEGAIWNINSFDQWGVELGK.V YFR028C 5 12 15.6% 551 61907 8.0 U CDC14 SGDID:S000001924, Chr VI from 210056-208401, reverse complement, Verified ORF, "Protein phosphatase required for mitotic exit; located in the nucleolus until liberated by the FEAR and Mitotic Exit Network in anaphase, enabling it to act on key substrates to effect a decrease in CDK/B-cyclin activity and mitotic exit" * Jamie_FT_01_itms_18.10677.10677.2 2.2965 0.2141 100.0 1472.4521 1470.6653 8219.6 1 4.98 59.1 2 R.SVYLDNTIEFLR.G * Jamie_FT_01_itms_10.05447.05447.2 2.1386 0.2309 100.0 1188.6122 1188.3256 7629.3 1 5.004 70.0 1 K.AVVFYSSASTR.Q * Jamie_FT_02_itms_19.14366.14366.2 3.4958 0.3055 100.0 2520.5723 2519.7295 11133.3 1 5.601 40.5 7 R.DAGYSNADFEITIQDVVYGVWR.A * Jamie_FT_01_itms_9.05239.05239.2 2.5373 0.0833 99.3 1243.8322 1243.3622 6684.8 20 3.772 65.0 1 R.VAQNNIEGELR.D * Jamie_FT_03_itms_3.09904.09904.3 1.9377 0.2333 94.8 3288.6243 3290.757 4869.7 58 4.378 18.1 1 R.QLLPKNRRVTSGRRTTSAAGGIRKIS*GSIK.K YJR139C 2 2 15.6% 359 38502 7.5 U HOM6 SGDID:S000003900, Chr X from 690439-689360, reverse complement, Verified ORF, "Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase), dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions" * Jamie_FT_01_itms_38.22397.22397.3 3.2164 0.1413 96.7 4011.4443 4011.385 9428.5 2 3.532 20.0 1 R.EIIQTGDEVEKIEGIFSGTLSYIFNEFSTSQANDVK.F * Jamie_FT_01_itms_17.10095.10095.3 3.1046 0.0877 92.9 2273.5745 2275.4314 8642.0 109 3.328 30.3 1 R.IS*GVEVESPT*SFPVQSLIPK.P Reverse_YIL041W 1 1 15.6% 326 36670 5.0 U GVP36 SGDID:S000001303, Chr IX from 276524-277504, Uncharacterized ORF, "Golgi vesicle protein of unknown function; localizes to both early and late Golgi vesicles" * Jamie_FT_02_itms_21.15618.15618.4 4.6837 0.0875 98.2 6141.7363 6143.575 9553.0 306 3.985 13.0 1 K.S*AVSRSFENVSENIYKPY*DYSGNEYIATVGLFHNYILKITDVKDELETYER.P YGR085C 2 3 15.5% 174 19750 9.9 U RPL11B SGDID:S000003317, Chr VII from 648911-648387, reverse complement, Verified ORF, "Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl11Ap; involved in ribosomal assembly; depletion causes degradation of proteins and RNA of the 60S subunit; has similarity to E. coli L5 and rat L11" YPR102C 2 3 15.5% 174 19720 9.9 U RPL11A SGDID:S000006306, Chr XVI from 731746-731222, reverse complement, Verified ORF, "Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl11Bp; involved in ribosomal assembly; depletion causes degradation of proteins and RNA of the 60S subunit; has similarity to E. coli L5 and rat L11" Jamie_FT_01_itms_13.07723.07723.2 3.4527 0.2894 100.0 1360.4521 1359.5223 8094.8 1 5.74 66.7 2 K.LVLNISVGESGDR.L Jamie_FT_01_itms_9.05353.05353.2 4.3151 0.3773 100.0 1514.2522 1514.719 8861.9 1 7.398 73.1 1 K.VLEQLSGQTPVQSK.A Reverse_YBL002W 1 1 15.3% 131 14237 10.1 U HTB2 SGDID:S000000098, Chr II from 236495-236890, Verified ORF, "One of two nearly identical (see HTB1) histone H2B subtypes required for chromatin assembly and chromosome function; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates transcriptional activation, meiotic DSB formation and H3 methylation" Reverse_YDR224C 1 1 15.3% 131 14252 10.1 U HTB1 SGDID:S000002632, Chr IV from 914706-914311, reverse complement, Verified ORF, "One of two nearly identical (see HTB2) histone H2B subtypes required for chromatin assembly and chromosome function; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates transcriptional activation, meiotic DSB formation and H3 methylation " Jamie_FT_01_itms_33.19284.19284.2 2.0598 0.1426 98.6 2267.112 2269.3035 7917.7 12 3.619 28.9 1 -.AQTSSSY*KT*VARTGESVAHK.A YDR064W 2 7 15.2% 151 17029 10.4 U RPS13 SGDID:S000002471, Chr IV from 579455-579475,580015-580449, Verified ORF, "Protein component of the small (40S) ribosomal subunit; has similarity to E. coli S15 and rat S13 ribosomal proteins" * Jamie_FT_01_itms_9.05375.05375.2 2.421 0.1569 99.8 1138.2322 1138.2657 4765.2 44 4.794 55.0 1 K.GISSSAIPYSR.N * Jamie_FT_01_itms_16.09393.09393.2 3.1986 0.2318 100.0 1332.3722 1332.537 10232.1 5 5.598 59.1 6 K.LSSESVIEQIVK.Y YDL130W 1 1 15.1% 106 10668 4.0 U RPP1B SGDID:S000002288, Chr IV from 229906-230019,230321-230527, Verified ORF, "Ribosomal protein P1 beta, component of the ribosomal stalk, which is involved in interaction of translational elongation factors with ribosome; accumulation is regulated by phosphorylation and interaction with the P2 stalk component" * Jamie_FT_01_itms_15.08419.08419.2 1.6867 0.0826 90.3 1664.1921 1664.815 8846.9 115 2.981 36.7 1 K.AAGANVDNVWADVYAK.A YDR453C 1 1 14.8% 196 21615 7.3 U TSA2 SGDID:S000002861, Chr IV from 1365652-1365062, reverse complement, Verified ORF, "Stress inducible cytoplasmic thioredoxin peroxidase; cooperates with Tsa1p in the removal of reactive oxygen, nitrogen and sulfur species using thioredoxin as hydrogen donor; deletion enhances the mutator phenotype of tsa1 mutants " * Jamie_FT_02_itms_25.18029.18029.2 1.4152 0.1836 95.7 3397.7522 3396.6946 9896.5 32 3.337 19.6 1 K.KFEDQGAQVLFAST*DSEYSLLAWTNLPRK.D YGR214W 3 5 14.7% 252 28024 4.7 U RPS0A SGDID:S000003446, Chr VII from 920580-920669,921125-921793, Verified ORF, "Protein component of the small (40S) ribosomal subunit, nearly identical to Rps0Bp; required for maturation of 18S rRNA along with Rps0Bp; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal" YLR048W 3 5 14.7% 252 27962 4.7 U RPS0B SGDID:S000004038, Chr XII from 242233-242322,242682-243350, Verified ORF, "Protein component of the small (40S) ribosomal subunit, nearly identical to Rps0Ap; required for maturation of 18S rRNA along with Rps0Ap; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal" Jamie_FT_01_itms_18.10782.10782.2 1.9131 0.0905 94.2 1765.4722 1766.0476 7023.5 13 4.053 40.6 1 R.IIAAIPNPEDVVAISSR.T Jamie_FT_01_itms_10.05477.05477.2 1.9533 0.116 98.6 913.0522 913.10504 5360.3 10 4.241 71.4 1 R.LVIVTDPR.S Jamie_FT_01_itms_23.13695.13695.2 3.3348 0.281 100.0 1443.8922 1442.7471 4597.4 1 7.17 86.4 3 K.HSIGLIWYLLAR.E YOR257W 2 6 14.3% 161 18751 4.6 U CDC31 SGDID:S000005783, Chr XV from 811006-811491, Verified ORF, "Component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; interacts with Kar1p" * Jamie_FT_01_itms_16.09065.09065.2 3.8587 0.3976 100.0 1381.3322 1380.5549 9844.9 1 7.453 80.0 5 K.YDDFYIVMGEK.I * Jamie_FT_01_itms_11.06548.06548.2 2.1459 0.196 99.8 1405.6522 1405.5016 6876.0 39 3.974 54.5 1 K.ELGETLTDEELR.A YDR308C 1 1 14.3% 140 16071 5.0 U SRB7 SGDID:S000002716, Chr IV from 1078443-1078021, reverse complement, Verified ORF, "Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; target of the global repressor Tup1p" * Jamie_FT_02_itms_12.10181.10181.2 2.0449 0.0476 92.3 2253.8123 2256.388 9415.7 102 3.235 31.6 1 R.HVDSLIEDFVDGIANS*KKST.- YBL072C 2 3 14.0% 200 22490 10.7 U RPS8A SGDID:S000000168, Chr II from 89123-88521, reverse complement, Verified ORF, "Protein component of the small (40S) ribosomal subunit; identical to Rps8Ap and has similarity to rat S8 ribosomal protein" YER102W 2 3 14.0% 200 22490 10.7 U RPS8B SGDID:S000000904, Chr V from 363096-363698, Verified ORF, "Protein component of the small (40S) ribosomal subunit; identical to Rps8Bp and has similarity to rat S8 ribosomal protein" Jamie_FT_01_itms_14.08052.08052.2 3.2117 0.3164 100.0 1303.8922 1302.5162 6423.4 1 5.348 68.2 2 K.AAIVQIDATPFR.Q Jamie_FT_02_itms_13.10802.10802.2 2.353 0.0859 97.5 1949.4521 1949.1334 11107.6 1 4.369 46.7 1 R.CDGYILEGEELAFYLR.R YML028W 2 3 13.8% 196 21590 5.1 U TSA1 SGDID:S000004490, Chr XIII from 220138-220728, Verified ORF, "Ubiquitous housekeeping thioredoxin peroxidase, reduces reactive oxygen, nitrogen and sulfur species using thioredoxin as hydrogen donor; mediates redox regulation of the nuclear localization of Yap1p; deletion results in mutator phenotype" * Jamie_FT_01_itms_38.22680.22680.2 3.8999 0.4938 100.0 3019.5322 3019.5598 6389.3 1 8.444 40.4 2 K.YVVLAFIPLAFTFVCPTEIIAFSEAAK.K * Jamie_FT_01_itms_38.22609.22609.3 3.5615 0.1527 98.2 3020.3643 3019.5598 10375.3 251 4.434 23.1 1 K.YVVLAFIPLAFTFVCPTEIIAFSEAAK.K YDL229W 6 9 13.7% 613 66602 5.4 U SSB1 SGDID:S000002388, Chr IV from 44066-45907, Verified ORF, "Cytoplasmic ATPase that is a ribosome-associated molecular chaperone; may be involved in the folding of newly-synthesized polypeptide chains; member of the heat shock protein 70 (HSP70) family; interacts with the phosphatase subunit Reg1p" * Jamie_FT_01_itms_13.07778.07778.2 2.4322 0.1342 99.5 1481.6721 1480.6604 6350.4 1 3.534 58.3 1 R.VTPSFVAFTPEER.L Jamie_FT_01_itms_14.08139.08139.2 2.8189 0.127 100.0 1186.4521 1186.3536 8025.7 5 4.353 68.2 1 K.DAGAISGLNVLR.I Jamie_FT_01_itms_16.09203.09203.2 1.5937 0.1185 92.7 1730.4722 1731.0018 7596.8 242 3.632 35.3 1 R.IINEPTAAAIAYGLGAGK.S Jamie_FT_02_itms_14.11351.11351.3 6.9486 0.477 100.0 2829.7144 2830.2993 5864.2 1 8.877 39.4 4 R.HVLIFDLGGGTFDVSLLHIAGGVYTVK.S Jamie_FT_02_itms_14.11291.11291.4 3.5165 0.0946 95.4 2830.1365 2830.2993 8364.0 16 2.941 27.6 1 R.HVLIFDLGGGTFDVSLLHIAGGVYTVK.S Jamie_FT_01_itms_13.07272.07272.2 2.5612 0.0857 98.7 1461.0322 1460.6273 7261.9 107 3.539 50.0 1 K.SQIDEVVLVGGSTR.I Reverse_YDR511W 1 1 13.5% 133 15783 9.1 U ACN9 SGDID:S000002919, Chr IV from 1470007-1470408, Verified ORF, "Protein of the mitochondrial intermembrane space, required for acetate utilization and gluconeogenesis; has orthologs in higher eukaryotes" * Jamie_FT_01_itms_30.17802.17802.2 2.1774 0.0793 96.1 2221.0322 2219.5515 7964.1 57 3.615 32.4 1 R.RYLVLPPLLLQSNHT*AFR.V YOR063W 2 2 13.4% 387 43758 10.3 U RPL3 SGDID:S000005589, Chr XV from 444687-445850, Verified ORF, "Protein component of the large (60S) ribosomal subunit, has similarity to E. coli L3 and rat L3 ribosomal proteins; involved in the replication and maintenance of killer double stranded RNA virus" * Jamie_FT_04_itms_6.14097.14097.3 2.4815 0.1489 91.0 2903.9343 2901.2212 9945.1 3 3.747 25.0 1 R.S*KPVALTSFLGYKAGMTT*IVRDLDR.P * Jamie_FT_01_itms_36.21593.21593.2 3.1753 0.3437 100.0 2811.8323 2810.2603 9365.0 5 5.816 26.9 1 R.EVVEAVTVVDTPPVVVVGVVGYVETPR.G Reverse_YOR286W 1 1 13.4% 149 16697 9.6 U YOR286W SGDID:S000005812, Chr XV from 850277-850726, Uncharacterized ORF, "The authentic, non-tagged protein was localized to the mitochondria" * Jamie_FT_01_itms_35.20553.20553.2 2.4646 0.1757 99.8 2360.8523 2363.593 7955.0 55 4.197 28.9 1 K.PAKTTFTRLVLT*PS*RASVTR.S YPR139C 1 1 13.3% 300 33816 9.7 U VPS66 SGDID:S000006343, Chr XVI from 814054-813152, reverse complement, Verified ORF, "Cytoplasmic protein of unknown function involved in vacuolar protein sorting." * Jamie_FT_02_itms_17.13265.13265.4 3.1866 0.1887 98.4 4771.8965 4766.52 9191.2 1 3.901 17.1 1 K.KFELQTIQIKTNKTAIT*TLPISNMEYLSRFLNKGINVKCK.I Reverse_YMR269W 1 1 13.3% 211 23949 10.1 U YMR269W SGDID:S000004882, Chr XIII from 804455-805090, Uncharacterized ORF, "Nucleolar protein of unknown function" * Jamie_FT_02_itms_18.14094.14094.3 2.7744 0.1229 91.2 3083.7544 3086.3433 8202.2 171 3.907 20.4 1 R.RKKGKSS*SASSVVFS*AEEKKGLNTITGK.L YDR450W 2 8 13.0% 146 17038 10.3 U RPS18A SGDID:S000002858, Chr IV from 1359913-1359959,1360395-1360788, Verified ORF, "Protein component of the small (40S) ribosomal subunit; nearly identical to Rps18Bp and has similarity to E. coli S13 and rat S18 ribosomal proteins" YML026C 2 8 13.0% 146 17038 10.3 U RPS18B SGDID:S000004488, Chr XIII from 223828-223782,223380-222987, reverse complement, Verified ORF, "Protein component of the small (40S) ribosomal subunit; nearly identical to Rps18Ap and has similarity to E. coli S13 and rat S18 ribosomal proteins" Jamie_FT_01_itms_9.05345.05345.2 3.5097 0.193 100.0 1202.5721 1201.3654 5419.4 1 5.788 80.0 1 R.LLNTNVDGNIK.I Jamie_FT_01_itms_16.09365.09365.2 2.5462 0.2341 100.0 1017.0722 1017.2187 5497.0 1 5.13 92.9 7 K.IPAWFLNR.Q YDL029W 1 1 12.8% 391 44074 5.8 U ARP2 SGDID:S000002187, Chr IV from 399337-399358,399482-400635, Verified ORF, "Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity" * Jamie_FT_02_itms_25.17777.17777.4 3.3566 0.1443 97.1 5224.0166 5223.9346 7755.9 1 3.463 15.0 1 K.YDFGGVYVAIQAVLALYAQGLSSGVVVDSGDGVTHIVPVYESVVLSHLTR.R YOR100C 1 1 12.8% 327 34754 9.8 U CRC1 SGDID:S000005626, Chr XV from 514278-513295, reverse complement, Verified ORF, "Mitochondrial inner membrane carnitine transporter, required for carnitine-dependent transport of acetyl-CoA from peroxisomes to mitochondria during fatty acid beta-oxidation" * Jamie_FT_02_itms_19.14673.14673.4 3.7667 0.1064 96.7 4551.9365 4555.092 9217.2 176 3.337 15.4 1 K.KLVT*FNNKQGGSNELT*MGQMAAAGFISAIPTTLVTAPTERVK.V YDR356W 10 22 12.5% 944 111782 7.1 U SPC110 SGDID:S000002764, Chr IV from 1186098-1188932, Verified ORF, "Inner plaque spindle pole body (SPB) component, ortholog of human kendrin; involved in connecting nuclear microtubules to SPB; interacts with Tub4p-complex and calmodulin; phosphorylated by Mps1p in cell cycle-dependent manner" * Jamie_FT_01_itms_18.10338.10338.2 2.8658 0.1858 100.0 1283.6522 1283.528 9221.5 4 4.934 70.0 2 K.NMEFTPVGFIK.S * Jamie_FT_01_itms_18.10764.10764.2 2.2138 0.2088 100.0 1860.6721 1861.0148 9259.6 4 4.155 40.0 1 R.LFSEASQFDDSFPEIK.A * Jamie_FT_01_itms_16.09301.09301.2 2.4757 0.1397 99.8 1218.1721 1218.4106 6985.6 5 3.874 77.8 2 K.NLMNELNELK.S * Jamie_FT_01_itms_10.05837.05837.2 3.8922 0.3789 100.0 1262.2122 1262.3997 8923.8 1 6.775 90.0 1 K.IAELEEEISTK.N * Jamie_FT_01_itms_20.11817.11817.2 2.8925 0.3442 100.0 1533.5521 1533.8236 8090.5 1 6.467 57.7 3 K.LASLMAQLTQLESK.L * Jamie_FT_01_itms_16.09173.09173.2 1.9559 0.1045 97.6 1218.6322 1219.4233 6038.1 57 4.206 61.1 1 K.QLENDLFVIK.K * Jamie_FT_01_itms_9.05112.05112.2 4.229 0.2963 100.0 1288.1921 1288.4435 8651.4 1 6.886 90.9 1 K.ISNLAAENSQLK.N * Jamie_FT_01_itms_9.05057.05057.2 2.6816 0.2361 100.0 1206.1322 1206.2921 5016.0 30 4.503 72.2 9 K.DSEDKIEELK.I * Jamie_FT_01_itms_10.05441.05441.2 2.8685 0.1488 100.0 1162.2922 1162.2823 4880.7 4 4.301 77.8 1 K.LQEDEISSLK.S * Jamie_FT_01_itms_15.08972.08972.2 1.5464 0.1486 94.4 1695.5721 1696.8113 8070.3 49 3.725 46.2 1 K.NYLSEITSLQEENR.R YFL039C 2 3 12.3% 375 41690 5.7 U ACT1 SGDID:S000001855, Chr VI from 54695-54686,54377-53260, reverse complement, Verified ORF, "Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions" * Jamie_FT_01_itms_21.12493.12493.3 3.0473 0.1066 93.6 3109.1343 3107.5798 9594.2 26 4.097 22.4 1 R.TTGIVLDSGDGVTHVVPIYAGFSLPHAILR.I * Jamie_FT_02_itms_7.07452.07452.2 2.049 0.1493 98.9 1791.1122 1791.9554 9968.4 9 4.29 46.7 2 K.SYELPDGQVITIGNER.F YJL191W 1 1 12.3% 138 14650 10.5 U RPS14B SGDID:S000003727, Chr X from 73786-73795,74204-74610, Verified ORF, "Ribosomal protein 59 of the small subunit, required for ribosome assembly and 20S pre-rRNA processing; mutations confer cryptopleurine resistance; nearly identical to Rps14Ap and similar to E. coli S11 and rat S14 ribosomal proteins" * Jamie_FT_02_itms_6.06320.06320.2 2.9832 0.2392 96.2 1890.4321 1890.0659 6760.7 1 6.026 50.0 1 A.NDLVQARDNSQVFGVAR.I YJL190C 1 1 12.3% 130 14626 9.9 U RPS22A SGDID:S000003726, Chr X from 75301-74909, reverse complement, Verified ORF, "Protein component of the small (40S) ribosomal subunit; nearly identical to Rps22Bp and has similarity to E. coli S8 and rat S15a ribosomal proteins" YLR367W 1 1 12.3% 130 14626 9.9 U RPS22B SGDID:S000004359, Chr XII from 856441-856573,857057-857316, Verified ORF, "Protein component of the small (40S) ribosomal subunit; nearly identical to Rps22Ap and has similarity to E. coli S8 and rat S15a ribosomal proteins" Jamie_FT_01_itms_22.12774.12774.2 2.0399 0.0887 95.7 1632.1522 1630.7954 6964.1 10 3.83 50.0 1 R.SSVLADALNAINNAEK.T YBR031W 3 7 12.2% 362 39092 10.6 U RPL4A SGDID:S000000235, Chr II from 300166-301254, Verified ORF, "N-terminally acetylated protein component of the large (60S) ribosomal subunit, nearly identical to Rpl4Bp and has similarity to E. coli L4 and rat L4 ribosomal proteins" YDR012W 3 7 12.2% 362 39062 10.6 U RPL4B SGDID:S000002419, Chr IV from 471850-472938, Verified ORF, "Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl4Ap and has similarity to E. coli L4 and rat L4 ribosomal proteins" Jamie_FT_02_itms_14.11463.11463.2 3.1176 0.2652 100.0 1820.5922 1821.1271 13060.5 1 5.401 50.0 4 R.YATASAIAATAVASLVLAR.G Jamie_FT_01_itms_19.11282.11282.2 2.8443 0.2353 100.0 1314.0922 1313.5388 8215.5 1 4.972 80.0 1 R.FVIWTEAAFTK.L Jamie_FT_01_itms_10.05841.05841.2 2.8269 0.2467 100.0 1509.3722 1507.6403 7280.0 1 6.287 65.4 2 K.LDQVWGSETVASSK.V YGL008C 8 13 12.0% 918 99619 5.1 U PMA1 SGDID:S000002976, Chr VII from 482671-479915, reverse complement, Verified ORF, "Plasma membrane H+-ATPase, pumps protons out of the cell; major regulator of cytoplasmic pH and plasma membrane potential; part of the P2 subgroup of cation-transporting ATPases" * Jamie_FT_01_itms_10.05490.05490.2 2.4655 0.1518 100.0 1058.5521 1058.2664 6101.7 1 4.209 83.3 1 K.TLANTAVVIR.D Jamie_FT_02_itms_24.17273.17273.3 3.5196 0.1717 98.7 3064.2244 3062.7217 10573.5 256 4.172 20.8 2 R.YTLGITIIGVPVGLPAVVTTTMAVGAAYLAK.K Jamie_FT_01_itms_37.21795.21795.2 2.9464 0.1458 100.0 3065.2122 3062.7217 7144.8 1 4.004 28.3 1 R.YTLGITIIGVPVGLPAVVTTTMAVGAAYLAK.K Jamie_FT_01_itms_20.11924.11924.2 1.959 0.2137 99.6 1805.6721 1806.0295 7818.4 2 4.898 40.6 1 K.LSAIESLAGVEILCSDK.T Jamie_FT_01_itms_12.06707.06707.2 2.7208 0.1417 100.0 1450.9122 1449.6066 4805.4 1 5.008 58.3 1 R.QLGLGTNIYNAER.L Jamie_FT_01_itms_10.05432.05432.2 2.4145 0.0201 97.5 971.3722 971.1447 7068.8 26 3.535 85.7 1 R.VVEILQNR.G Jamie_FT_01_itms_33.19685.19685.2 3.5709 0.3387 100.0 1987.6522 1986.3593 4630.6 1 5.927 57.9 5 R.SAADIVFLAPGLSAIIDALK.T Jamie_FT_01_itms_16.09249.09249.2 1.883 0.0779 94.2 1285.5521 1285.4756 9553.8 23 3.646 55.0 1 R.SVEDFMAAMQR.V YLR303W 3 6 11.7% 444 48672 6.4 U MET17 SGDID:S000004294, Chr XII from 732544-733878, Verified ORF, "O-acetyl homoserine-O-acetyl serine sulfhydrylase, required for sulfur amino acid synthesis" * Jamie_FT_01_itms_38.22615.22615.2 5.1935 0.4428 100.0 2635.4722 2633.121 10684.2 1 8.198 50.0 4 R.DLGPLMNPFASFLLLQGVETLSLR.A * Jamie_FT_02_itms_32.22302.22302.2 2.0836 0.0922 96.3 3101.652 3102.4675 10743.1 49 3.273 24.1 1 R.VSVGIEFIDDIIADFQQSFETVFAGQKP.- * Jamie_FT_02_itms_32.22299.22299.3 5.4218 0.2169 100.0 3105.8044 3102.4675 8984.0 1 6.36 31.5 1 R.VSVGIEFIDDIIADFQQSFETVFAGQKP.- YKL152C 2 3 11.7% 247 27609 8.8 U GPM1 SGDID:S000001635, Chr XI from 164390-163647, reverse complement, Verified ORF, "Tetrameric phosphoglycerate mutase, mediates the conversion of 3-phosphoglycerate to 2-phosphoglycerate during glycolysis and the reverse reaction during gluconeogenesis" * Jamie_FT_01_itms_19.11388.11388.2 2.2947 0.0894 98.7 1179.9521 1179.3611 8266.3 1 4.233 77.8 2 K.NLFTGWVDVK.L * Jamie_FT_01_itms_22.12783.12783.2 2.319 0.127 99.0 2146.0122 2144.4294 8222.8 42 4.128 38.9 1 K.YVDPNVLPETESLALVIDR.L Reverse_YHR105W 1 1 11.7% 214 24646 8.3 U YPT35 SGDID:S000001147, Chr VIII from 324769-325413, Uncharacterized ORF, "Endosomal protein of unknown function that contains a phox (PX) homology domain and binds to both phosphatidylinositol-3-phosphate (PtdIns(3)P) and proteins involved in ER-Golgi or vesicular transport" * Jamie_FT_01_itms_24.14378.14378.3 3.1011 0.1539 96.6 3198.6843 3194.6646 7261.4 25 3.747 26.0 1 K.ATKIRT*LLNERLRVFDSYRKYTQIK.Y Reverse_YMR158W 1 1 11.6% 155 17471 9.6 U MRPS8 SGDID:S000004767, Chr XIII from 572247-572714, Verified ORF, "Mitochondrial ribosomal protein of the small subunit" * Jamie_FT_04_itms_12.17365.17365.2 1.6262 0.1075 92.5 2211.3523 2209.55 10131.9 22 2.906 35.3 1 R.ITVGS*CLKKMDEMPLHIR.S YKR104W 1 1 11.4% 306 34652 7.8 U YKR104W SGDID:S000001812, Chr XI from 656474-657394, Verified ORF, "ORFs YKR103W and YKR104W are merged in different strain backgrounds" * Jamie_FT_03_itms_28.25524.25524.3 2.0026 0.2609 96.9 4093.5842 4089.598 6141.9 183 4.654 12.5 1 R.S*MLGARNIMLIDEATAS*IDYISDAKIQKTIRETMK.N YGL030W 1 1 11.4% 105 11415 9.8 U RPL30 SGDID:S000002998, Chr VII from 439096-439098,439329-439643, Verified ORF, "Protein component of the large (60S) ribosomal subunit, has similarity to rat L30 ribosomal protein; involved in pre-rRNA processing in the nucleolus; autoregulates splicing of its transcript" * Jamie_FT_01_itms_15.08532.08532.2 2.4905 0.2185 100.0 1294.1322 1294.6237 5925.4 2 5.275 68.2 1 K.LIIIAANTPVLR.K YNL055C 1 1 11.0% 283 30428 7.9 U POR1 SGDID:S000005000, Chr XIV from 518846-517995, reverse complement, Verified ORF, "Mitochondrial porin (voltage-dependent anion channel), outer membrane protein required for the maintenance of mitochondrial osmotic stability and mitochondrial membrane permeability" * Jamie_FT_02_itms_20.15264.15264.3 3.6741 0.0792 94.5 3420.0544 3419.7722 11781.4 194 3.549 20.8 1 K.DYSLGATLNNEQITTVDFFQNVNAFLQVGAK.A YJR077C 1 2 10.9% 311 32812 9.3 U MIR1 SGDID:S000003838, Chr X from 578104-577169, reverse complement, Verified ORF, "Mitochondrial phosphate carrier, imports inorganic phosphate into mitochondria; functionally redundant with Pic2p but more abundant than Pic2 under normal conditions" * Jamie_FT_01_itms_43.25592.25592.2 2.6982 0.277 100.0 3329.152 3329.8564 7653.1 1 6.976 22.7 2 K.LSSTSTTLLNLLSGLTAGLAAAIVSQPADTLLSK.V Reverse_YBL093C 1 1 10.9% 220 24857 6.4 U ROX3 SGDID:S000000189, Chr II from 44915-44253, reverse complement, Verified ORF, "RNA polymerase II holoenzyme component" * Jamie_FT_02_itms_18.14105.14105.2 1.858 0.1738 98.7 2756.9321 2755.911 10903.7 94 4.657 28.3 1 R.RKMDEHSDSHTPTAMSSGS*SKNKR.K YBR164C 1 2 10.9% 183 20434 4.9 U ARL1 SGDID:S000000368, Chr II from 568421-567870, reverse complement, Verified ORF, "Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor" * Jamie_FT_01_itms_34.20197.20197.2 2.6814 0.0517 97.5 2259.2322 2258.5303 11425.7 1 3.48 44.7 2 K.GEGITEGLDWLIDVIKEEQL.- Reverse_YDL136W 1 1 10.8% 120 13910 10.6 U RPL35B SGDID:S000002295, Chr IV from 217600-217602,218008-218367, Verified ORF, "Protein component of the large (60S) ribosomal subunit, identical to Rpl35Ap and has similarity to rat L35 ribosomal protein" Reverse_YDL191W 1 1 10.8% 120 13910 10.6 U RPL35A SGDID:S000002350, Chr IV from 117665-117667,118159-118518, Verified ORF, "Protein component of the large (60S) ribosomal subunit, identical to Rpl35Bp and has similarity to rat L35 ribosomal protein" Jamie_FT_05_itms_22.28645.28645.2 2.0124 0.1391 98.4 1514.0721 1514.8491 6608.2 49 3.599 54.2 1 R.SLKQVKLEALEKK.L YBR221C 1 1 10.7% 366 40054 5.3 U PDB1 SGDID:S000000425, Chr II from 666248-665148, reverse complement, Verified ORF, "E1 beta subunit of the pyruvate dehydrogenase (PDH) complex, which is an evolutionarily-conserved multi-protein complex found in mitochondria" * Jamie_FT_01_itms_34.20354.20354.3 4.6132 0.3427 100.0 4286.9644 4284.784 9078.1 1 6.38 25.0 1 K.TNHLITVESTFPSFGVGAEIVAQVMESEAFDYLDAPIQR.V YNL126W 4 6 10.6% 846 98227 7.5 U SPC98 SGDID:S000005070, Chr XIV from 387229-389769, Verified ORF, "Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque" * Jamie_FT_01_itms_39.22841.22841.3 6.2852 0.4526 100.0 2824.9744 2824.292 10182.4 1 7.621 42.7 2 K.IPNFESGLLHLIFEAGLLYQSLGYK.V * Jamie_FT_01_itms_39.22854.22854.2 3.7143 0.1392 100.0 2825.4922 2824.292 9259.6 1 4.052 39.6 1 K.IPNFESGLLHLIFEAGLLYQSLGYK.V * Jamie_FT_04_itms_16.19932.19932.3 4.0007 0.257 100.0 3256.7043 3253.7173 9881.6 119 5.77 23.3 1 K.ALIIEISEELQNYTAFVNNLVSSGTVVSLK.S * Jamie_FT_02_itms_23.16841.16841.3 4.5171 0.2891 100.0 3855.2344 3854.3455 10046.6 1 5.052 20.6 2 K.LFATNTSEISVGDYSGQPYPTSLVLLLNSVYEFVK.V Reverse_YGL003C 2 2 10.2% 566 62822 8.6 U CDH1 SGDID:S000002971, Chr VII from 494178-492478, reverse complement, Verified ORF, "Cell-cycle regulated activator of the anaphase-promoting complex/cyclosome (APC/C), which directs ubiquitination of mitotic cyclins resulting in exit from mitosis; targets the APC/C to specific substrates including CDC20, ASE1 and CIN8" * Jamie_FT_03_itms_12.15590.15590.2 1.526 0.1763 96.4 2191.172 2192.4143 7265.6 24 4.413 32.5 1 R.DATGGGT*ALVGRKHPSWAMAK.V * Jamie_FT_03_itms_18.19642.19642.3 2.2643 0.2548 97.9 4082.3943 4086.3389 10632.0 149 4.53 17.4 1 R.DGYVTSPRSRRSPSSLLSASS*SSSIPRKS*VRKSESGK.L YJL167W 1 1 10.2% 352 40483 5.5 U ERG20 SGDID:S000003703, Chr X from 105007-106065, Verified ORF, "Farnesyl pyrophosphate synthetase, has both dimethylallyltranstransferase and geranyltranstransferase activities; catalyzes the formation of C15 farnesyl pyrophosphate units for isoprenoid and sterol biosynthesis" * Jamie_FT_01_itms_36.21169.21169.3 4.8471 0.2608 100.0 4219.8545 4217.7744 8687.3 37 5.179 17.1 1 K.YYIDITELFHEVTFQTELGQLMDLITAPEDKVDLSK.F YJL026W 2 3 10.0% 399 46147 5.2 U RNR2 SGDID:S000003563, Chr X from 392320-393519, Verified ORF, "Ribonucleotide-diphosphate reductase (RNR), small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits" * Jamie_FT_01_itms_18.10394.10394.2 1.7865 0.0776 92.7 1422.7122 1421.5522 9035.9 2 3.698 54.5 1 R.WIQDADALFGER.L * Jamie_FT_01_itms_38.22493.22493.2 3.8544 0.3245 100.0 3191.6921 3190.6992 7495.0 1 8.117 37.0 2 R.YFLDALPVALLGMNADLMNQYVEFVADR.L YDR382W 1 1 10.0% 110 11050 4.1 U RPP2B SGDID:S000002790, Chr IV from 1239482-1239814, Verified ORF, "Ribosomal protein P2 beta, a component of the ribosomal stalk, which is involved in the interaction between translational elongation factors and the ribosome; regulates the accumulation of P1 (Rpp1Ap and Rpp1Bp) in the cytoplasm" * Jamie_FT_01_itms_14.07903.07903.2 2.3727 0.1785 100.0 1203.0322 1203.3782 7915.4 11 3.762 65.0 1 R.INELLSSLEGK.G Reverse_YKL077W 1 1 9.9% 392 46036 8.9 U YKL077W SGDID:S000001560, Chr XI from 291097-292275, Uncharacterized ORF, "Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole" * Jamie_FT_01_itms_28.16501.16501.4 3.8524 0.0501 93.3 4745.1367 4750.321 8763.6 236 3.093 15.8 1 K.VEESTT*MKEKPRVVQRAGHVHQYLSALS*FFILISDHFRM.- YHL015W 1 4 9.9% 121 13907 9.5 U RPS20 SGDID:S000001007, Chr VIII from 75409-75774, Verified ORF, "Protein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; has similarity to E. coli S10 and rat S20 ribosomal proteins" * Jamie_FT_01_itms_16.09066.09066.2 2.3977 0.0298 95.6 1388.0521 1388.647 7114.6 1 4.436 54.5 4 R.YIDLEAPVQIVK.R Reverse_YPL192C 1 1 9.8% 133 14428 10.3 U PRM3 SGDID:S000006113, Chr XVI from 183055-182654, reverse complement, Verified ORF, "Pheromone-regulated protein required for karyogamy; localizes to the inner membrane of the nuclear envelope" * Jamie_FT_02_itms_9.08436.08436.2 1.5602 0.1472 95.3 1623.5721 1623.634 10137.3 3 4.162 45.8 1 K.TRS*KTSSTT*TKHK.R YDR055W 1 1 9.5% 444 45777 9.2 U PST1 SGDID:S000002462, Chr IV from 563524-564858, Verified ORF, "Cell wall protein that contains a putative GPI-attachment site; secreted by regenerating protoplasts; up-regulated by activation of the cell integrity pathway, as mediated by Rlm1p; upregulated by cell wall damage via disruption of FKS1" * Jamie_FT_01_itms_27.16207.16207.4 4.2474 0.3025 100.0 4399.9766 4400.943 7899.8 50 5.197 16.7 1 V.GNFTSLNLDSLKSVKGGADVESKSSNFSCNALKALQKKGGIK.G Reverse_YGR122W 1 1 9.5% 402 45305 8.2 U YGR122W SGDID:S000003354, Chr VII from 733940-735148, Uncharacterized ORF, "Hypothetical protein" * Jamie_FT_02_itms_22.15993.15993.4 3.9764 0.1191 97.6 4365.3765 4360.7515 10393.0 276 3.252 16.2 1 K.LWTDICTISDAKSSSSNLSNICNNRLIILDNPIS*TTIK.S YGL209W 1 1 9.4% 382 42048 9.7 U MIG2 SGDID:S000003177, Chr VII from 95862-97010, Verified ORF, "Protein containing zinc fingers, involved in repression, along with Mig1p, of SUC2 (invertase) expression by high levels of glucose; binds to Mig1p-binding sites in SUC2 promoter" * Jamie_FT_02_itms_12.10388.10388.4 3.2637 0.0805 90.7 4187.2563 4186.377 7360.2 70 3.069 18.6 1 R.VNT*PRSVPNSPNDGYLHQQHIPQQY*QHQTASPSVAK.Q Reverse_YHR060W 1 1 9.4% 181 21078 5.5 U VMA22 SGDID:S000001102, Chr VIII from 220727-221272, Verified ORF, "Integral membrane protein that is required for vacuolar H+-ATPase (V-ATPase) function, although not an actual component of the V-ATPase complex; functions in the assembly of the V-ATPase; localized to the yeast endoplasmic reticulum (ER)" * Jamie_FT_04_itms_6.14352.14352.2 1.3809 0.1824 95.1 2103.5322 2103.3896 6637.3 301 3.381 31.2 1 K.LKHSQTKQKEPKT*GKKR.Q YOR234C 1 1 9.3% 107 12168 11.1 U RPL33B SGDID:S000005760, Chr XV from 779405-779387,778859-778555, reverse complement, Verified ORF, "Ribosomal protein L37 of the large (60S) ribosomal subunit, nearly identical to Rpl33Ap and has similarity to rat L35a; rpl33b null mutant exhibits normal growth while rpl33a rpl33b double null mutant is inviable" YPL143W 1 1 9.3% 107 12154 11.1 U RPL33A SGDID:S000006064, Chr XVI from 282121-282139,282665-282969, Verified ORF, "N-terminally acetylated ribosomal protein L37 of the large (60S) ribosomal subunit, nearly identical to Rpl33Bp and has similarity to rat L35a; rpl33a null mutant exhibits slow growth while rpl33a rpl33b double null mutant is inviable" Jamie_FT_01_itms_9.05343.05343.2 2.0216 0.0978 97.6 1098.1322 1098.2877 5730.4 41 3.304 66.7 1 R.VNNPNVSLIK.I YGR027C 1 1 9.3% 108 12039 10.3 U RPS25A SGDID:S000003259, Chr VII from 534462-534136, reverse complement, Verified ORF, "Protein component of the small (40S) ribosomal subunit; nearly identical to Rps25Bp and has similarity to rat S25 ribosomal protein" YLR333C 1 1 9.3% 108 12009 10.3 U RPS25B SGDID:S000004325, Chr XII from 795899-795573, reverse complement, Verified ORF, "Protein component of the small (40S) ribosomal subunit; nearly identical to Rps25Ap and has similarity to rat S25 ribosomal protein" Jamie_FT_01_itms_13.07677.07677.2 2.4591 0.0984 99.5 1137.4321 1137.3213 7101.6 8 4.258 83.3 1 R.YVSVSVLVDR.L Reverse_YLR390W-A 2 2 9.2% 238 23268 5.9 U CCW14 SGDID:S000006429, Chr XII from 903724-904440, Verified ORF, "Covalently linked cell wall glycoprotein, present in the inner layer of the cell wall" * Jamie_FT_02_itms_35.23940.23940.2 1.7952 0.1127 94.4 2060.132 2060.9915 9879.1 11 3.736 31.0 1 K.TSSSASSSSAKTSSSASSSS*AK.T * Jamie_FT_04_itms_21.23047.23047.4 3.5858 0.0813 95.4 2135.9766 2140.9915 8780.4 126 3.589 30.2 1 K.TSSSASSSSAKT*SSS*ASSSSAK.T YOR217W 2 2 9.1% 861 94903 9.2 U RFC1 SGDID:S000005743, Chr XV from 749301-751886, Verified ORF, "Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon" * Jamie_FT_01_itms_35.20754.20754.2 1.9459 0.0757 93.8 3032.9521 3034.5818 8346.8 1 2.954 27.8 1 K.TPKKMPVSNVIDVSETPEGEKKLPLPAK.R * Jamie_FT_02_itms_19.14186.14186.4 3.0321 0.1221 94.6 5525.7363 5520.0483 9698.3 207 3.088 13.3 1 K.ENAKFDFKSANSNADPDEIVSEIGS*FPEGKPNCLLGLTIVFTGVLPTLER.G YER043C 1 1 9.1% 449 49126 6.2 U SAH1 SGDID:S000000845, Chr V from 237118-235769, reverse complement, Verified ORF, "S-adenosyl-L-homocysteine hydrolase, catabolizes S-adenosyl-L-homocysteine which is formed after donation of the activated methyl group of S-adenosyl-L-methionine (AdoMet) to an acceptor" * Jamie_FT_01_itms_34.20047.20047.3 3.8539 0.2646 100.0 4466.574 4467.0317 7158.0 52 4.915 16.2 1 R.VLVTEIDPINALQAAMEGYQVVTMEDASHIGQVFVTTTGCR.D YFR031C-A 2 4 9.1% 254 27408 11.1 U RPL2A SGDID:S000002104, Chr VI from 221406-221403,221255-220495, reverse complement, Verified ORF, "Protein component of the large (60S) ribosomal subunit, identical to Rpl2Bp and has similarity to E. coli L2 and rat L8 ribosomal proteins" YIL018W 2 4 9.1% 254 27408 11.1 U RPL2B SGDID:S000001280, Chr IX from 316766-316769,317170-317930, Verified ORF, "Protein component of the large (60S) ribosomal subunit, identical to Rpl2Ap and has similarity to E. coli L2 and rat L8 ribosomal proteins; expression is upregulated at low temperatures" Jamie_FT_01_itms_10.05760.05760.2 2.6502 0.3367 100.0 955.7922 956.1331 7889.8 1 6.135 80.0 3 R.GVIGVIAGGGR.V Jamie_FT_02_itms_5.05777.05777.2 2.049 0.1313 98.7 1303.7522 1304.4459 6900.2 124 3.861 50.0 1 R.TGLLRGSQKTQD.- YDL150W 1 1 9.0% 422 46667 8.6 U RPC53 SGDID:S000002309, Chr IV from 183344-184612, Verified ORF, "RNA polymerase III subunit C53" * Jamie_FT_03_itms_8.13592.13592.4 3.0218 0.1583 96.7 3974.5366 3969.4685 8472.7 1 3.422 20.3 1 -.MSSNKGNGRLPSLKDSSSNGGGS*AKPSLKFKPKAVARK.S YEL051W 1 3 9.0% 256 29194 5.9 U VMA8 SGDID:S000000777, Chr V from 58378-59148, Verified ORF, "Subunit D of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; plays a role in the coupling of proton transport and ATP hydrolysis" * Jamie_FT_01_itms_41.24206.24206.3 4.9438 0.4401 100.0 2516.7844 2516.978 9810.9 1 6.474 33.0 3 R.AVETLVELASLQTAFIILDEVIK.V YOR373W 4 13 8.9% 851 94104 7.0 U NUD1 SGDID:S000005900, Chr XV from 1036830-1039385, Verified ORF, "Component of the spindle pole body outer plaque, required for exit from mitosis" * Jamie_FT_02_itms_25.18047.18047.4 3.6205 0.1063 96.3 5069.5767 5067.7515 9451.8 1 3.87 17.0 1 K.KLNLSNNEINGIIDFEQLILTNNSVVGGWLTVEVLDLSNNNIIGVR.N * Jamie_FT_02_itms_28.19699.19699.3 4.4396 0.2467 100.0 4942.1343 4939.577 8842.2 1 6.432 18.2 3 K.LNLSNNEINGIIDFEQLILTNNSVVGGWLTVEVLDLSNNNIIGVR.N * Jamie_FT_01_itms_21.12581.12581.2 2.757 0.2766 100.0 1784.6322 1784.063 9757.9 1 5.312 43.8 3 K.VLNLNGNPLVSIVESSK.M * Jamie_FT_01_itms_15.08673.08673.2 3.6254 0.3872 100.0 1452.2722 1452.6506 9489.6 1 6.079 66.7 6 K.STNLYSLTIANVR.D YGR282C 1 1 8.9% 313 34119 4.5 U BGL2 SGDID:S000003514, Chr VII from 1058730-1057789, reverse complement, Verified ORF, "Endo-beta-1,3-glucanase, major protein of the cell wall, involved in cell wall maintenance" * Jamie_FT_02_itms_13.11106.11106.3 3.6619 0.2224 100.0 3142.0444 3142.4053 6053.7 1 4.9 32.4 1 R.AWGVNVIVFEAFDEDWKPNTSGTSDVEK.H YGL202W 1 1 8.8% 500 56178 6.0 U ARO8 SGDID:S000003170, Chr VII from 116063-117565, Verified ORF, "Aromatic aminotransferase, expression is regulated by general control of amino acid biosynthesis" * Jamie_FT_02_itms_39.26143.26143.4 1.7729 0.2285 90.8 5156.1367 5160.903 6622.4 172 3.483 12.8 1 K.ILKPYLS*LHEMTIQAPAGFTQVLVNAT*LSRWGQKGYLDWLLGLR.H YDL137W 1 1 8.8% 181 20658 7.4 U ARF2 SGDID:S000002296, Chr IV from 216529-217074, Verified ORF, "ADP-ribosylation factor, GTPase of the Ras superfamily involved in regulation of coated formation vesicles in intracellular trafficking within the Golgi; functionally interchangeable with Arf1p" * Jamie_FT_01_itms_51.30374.30374.2 2.249 0.0456 93.9 1871.2322 1871.118 6626.2 170 3.628 33.3 1 -.MGLY*ASKLFSNLFGNK.E YDR447C 1 1 8.8% 136 15803 10.5 U RPS17B SGDID:S000002855, Chr IV from 1355543-1355541,1355226-1354819, reverse complement, Verified ORF, "Ribosomal protein 51 (rp51) of the small (40s) subunit; nearly identical to Rps17Ap and has similarity to rat S17 ribosomal protein" YML024W 1 1 8.8% 136 15788 10.5 U RPS17A SGDID:S000004486, Chr XIII from 225889-225891,226290-226697, Verified ORF, "Ribosomal protein 51 (rp51) of the small (40s) subunit; nearly identical to Rps17Bp and has similarity to rat S17 ribosomal protein" Jamie_FT_01_itms_13.07649.07649.2 2.6113 0.2854 100.0 1297.1921 1297.5405 4449.9 8 5.385 54.5 1 K.LPLSVINVSAQR.D YCL028W 1 1 8.6% 405 42580 6.6 U RNQ1 SGDID:S000000533, Chr III from 70150-71367, Verified ORF, "[PIN(+)] prion, an infectious protein conformation that is generally an ordered protein aggregate" * Jamie_FT_01_itms_35.20779.20779.2 4.1109 0.4342 100.0 3344.612 3342.7812 10430.0 1 7.204 32.4 1 R.GFDVGTVMSMLSGSGGGSQSMGASGLAALASQFFK.S YDR098C-B 10 20 8.5% 1755 198594 8.4 U YDR098C-B SGDID:S000007391, Chr IV from 651121-649817,649815-645853, reverse complement, transposable_element_gene, "TyB Gag-Pol protein; proteolytically processed to make the Gag, RT, PR, and IN proteins that are required for retrotransposition" YER138C 10 20 8.5% 1755 198561 8.1 U YER138C SGDID:S000000940, Chr V from 449020-447719,447717-443752, reverse complement, transposable_element_gene, "TyB Gag-Pol protein; proteolytically processed to make the Gag, RT, PR, and IN proteins that are required for retrotransposition" YDR365W-B 10 20 8.5% 1755 198658 8.0 U YDR365W-B SGDID:S000007401, Chr IV from 1206987-1208291,1208293-1212255, transposable_element_gene, "TyB Gag-Pol protein; proteolytically processed to make the Gag, RT, PR, and IN proteins that are required for retrotransposition" YDR316W-B 10 20 8.5% 1755 198526 8.2 U YDR316W-B SGDID:S000007399, Chr IV from 1096059-1097363,1097365-1101327, transposable_element_gene, "TyB Gag-Pol protein; proteolytically processed to make the Gag, RT, PR, and IN proteins that are required for retrotransposition" YDR261C-D 10 20 9.4% 1604 181660 7.5 U YDR261C-D SGDID:S000007395, Chr IV from 992343-991039,991037-987528, reverse complement, transposable_element_gene, "TyB Gag-Pol protein; proteolytically processed to make the Gag, RT, PR, and IN proteins that are required for retrotransposition" YDR210C-D 10 20 8.5% 1755 198838 8.4 U YDR210C-D SGDID:S000007410, Chr IV from 883922-882618,882616-878654, reverse complement, transposable_element_gene, "TyB Gag-Pol protein; proteolytically processed to make the Gag, RT, PR, and IN proteins that are required for retrotransposition" Jamie_FT_01_itms_16.09014.09014.3 3.9949 0.072 96.5 2126.2144 2125.4788 8762.9 2 3.633 36.8 1 K.FLQNSNLGGIIPTVNGKPVR.Q Jamie_FT_01_itms_38.22433.22433.2 4.1671 0.32 100.0 3366.3123 3364.8218 5975.0 1 7.052 37.0 4 R.QITDDELTFLYNTFQIFAPSQFLPTWVK.D Jamie_FT_01_itms_37.22171.22171.3 4.4239 0.1537 99.6 3367.9744 3364.8218 9783.8 1 4.494 26.9 2 R.QITDDELTFLYNTFQIFAPSQFLPTWVK.D Jamie_FT_01_itms_19.10956.10956.2 2.94 0.0892 99.6 1414.7722 1413.6257 10417.7 44 3.998 59.1 3 K.DILSVDYTDIMK.I Jamie_FT_02_itms_15.11783.11783.3 4.1998 0.3007 100.0 2817.6243 2816.161 8048.7 1 6.196 31.5 2 R.HLNMTVAELFLDIHAIYEEQQGSR.N Jamie_FT_03_itms_13.16253.16253.3 3.8244 0.289 100.0 3104.6042 3104.5366 10533.7 1 5.998 27.8 2 K.VLHTPNIAYDLLSLNELAAVDITACFTK.N Jamie_FT_01_itms_38.22607.22607.3 4.1862 0.4448 100.0 1982.6643 1982.3275 10476.3 1 8.007 43.8 1 R.REDSILDVFTTILAFIK.N Jamie_FT_01_itms_38.22709.22709.2 5.203 0.462 100.0 1983.3922 1982.3275 7880.3 1 8.397 75.0 2 R.REDSILDVFTTILAFIK.N Jamie_FT_01_itms_43.25760.25760.2 4.5016 0.2288 100.0 1828.2322 1826.14 8964.8 1 6.108 66.7 2 R.EDSILDVFTTILAFIK.N Jamie_FT_01_itms_36.21479.21479.3 4.3439 0.4266 100.0 2628.0544 2629.0793 8226.8 1 7.588 35.0 1 K.FRFDLLYYINTLAQHILFPSR.Q YAR002W 2 2 8.5% 539 59039 9.3 U NUP60 SGDID:S000000063, Chr I from 152259-153878, Verified ORF, "Subunit of the nuclear pore complex (NPC), functions to anchor Nup2p to the NPC in a dynamic process that is controlled by the nucleoplasmic concentration of Gsp1p-GTP; potential Cdc28p substrate" * Jamie_FT_02_itms_19.14318.14318.2 1.6316 0.1329 94.3 2850.7522 2849.0842 9031.2 101 2.693 23.1 1 K.KIPDTYT*ANTSAQSIASAKSVRSGVSK.S * Jamie_FT_02_itms_16.12823.12823.2 1.734 0.089 92.2 2085.1921 2085.2375 7922.2 293 3.672 27.8 1 K.NNAASELANPYSSYVSQIR.K YGR234W 3 8 8.5% 399 44646 6.3 U YHB1 SGDID:S000003466, Chr VII from 959908-961107, Verified ORF, "Nitric oxide oxidoreductase, flavohemoglobin involved in nitric oxide detoxification; plays a role in the oxidative and nitrosative stress responses" * Jamie_FT_01_itms_40.23573.23573.4 5.842 0.4104 100.0 3661.7766 3662.1743 9290.7 1 6.963 25.3 1 K.AIKEVLGDAATPEIINAWGEAYQAIADIFITVEK.K * Jamie_FT_01_itms_44.25847.25847.2 3.9373 0.4515 100.0 3348.9722 3349.762 8395.8 1 8.499 35.0 3 K.EVLGDAATPEIINAWGEAYQAIADIFITVEK.K * Jamie_FT_02_itms_28.20118.20118.3 5.7389 0.3597 100.0 3349.9744 3349.762 11670.4 1 5.699 30.8 4 K.EVLGDAATPEIINAWGEAYQAIADIFITVEK.K YLR105C 1 1 8.5% 377 44109 8.4 U SEN2 SGDID:S000004095, Chr XII from 348181-347048, reverse complement, Verified ORF, "Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p; Sen2p contains the active site for tRNA 5' splice site cleavage and has similarity to Sen34p and to Archaeal tRNA splicing endonuclease" * Jamie_FT_01_itms_25.14510.14510.4 3.3783 0.0819 93.6 3700.7766 3702.906 7479.3 177 3.249 18.8 1 K.ARTEARLGLNDTPLHNRGGT*KS*NTETEMTLEK.V YBL030C 1 1 8.5% 318 34426 9.8 U PET9 SGDID:S000000126, Chr II from 164000-163044, reverse complement, Verified ORF, "Major ADP/ATP carrier of the mitochondrial inner membrane, exchanges cytosolic ADP for mitochondrially synthesized ATP; required for viability in many common lab strains carrying a mutation in the polymorphic SAL1 gene" * Jamie_FT_01_itms_33.19537.19537.2 4.0099 0.4942 100.0 2794.1921 2792.1663 8729.6 1 8.063 38.5 1 K.WFAGNLASGGAAGALSLLFVYSLDYAR.T YKR072C 1 1 8.4% 562 62478 4.7 U SIS2 SGDID:S000001780, Chr XI from 577765-576077, reverse complement, Verified ORF, "Negative regulatory subunit of the protein phosphatase 1 Ppz1p; involved in ion homeostasis and cell cycle progression" * Jamie_FT_01_itms_34.19929.19929.4 3.7811 0.1154 97.1 5064.6567 5066.4414 9978.0 368 2.857 13.4 1 K.SPNSNPAPVSNSIPGNHAVIPNHTNTS*RTTQLSGSPLVNEMKDYDPK.K Reverse_YNL151C 1 1 8.4% 251 27724 4.4 U RPC31 SGDID:S000005095, Chr XIV from 348523-347768, reverse complement, Verified ORF, "RNA polymerase III subunit C31; contains HMG-like C-terminal domain" * Jamie_FT_02_itms_38.25664.25664.2 1.7834 0.0707 90.4 2199.9922 2199.316 11155.2 94 3.045 27.5 1 K.GVDGYGLGFPLNSMY*NSGGGR.S YDL083C 1 5 8.4% 143 15847 10.3 U RPS16B SGDID:S000002241, Chr IV from 307789-307766,307333-306926, reverse complement, Verified ORF, "Protein component of the small (40S) ribosomal subunit; identical to Rps16Ap and has similarity to E. coli S9 and rat S16 ribosomal proteins" YMR143W 1 5 8.4% 143 15847 10.3 U RPS16A SGDID:S000004751, Chr XIII from 551927-551950,552495-552902, Verified ORF, "Protein component of the small (40S) ribosomal subunit; identical to Rps16Bp and has similarity to E. coli S9 and rat S16 ribosomal proteins" Jamie_FT_01_itms_21.12294.12294.2 3.8706 0.3197 100.0 1361.3322 1359.6488 5415.3 1 5.72 72.7 5 K.VYEPLLLVGLDK.F YLR249W 6 14 8.2% 1044 115945 6.0 U YEF3 SGDID:S000004239, Chr XII from 636782-639916, Verified ORF, "Translational elongation factor, stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes by releasing EF-1 alpha from the ribosomal complex; contains two ABC cassettes; binds and hydrolyses ATP" * Jamie_FT_01_itms_37.21757.21757.3 5.595 0.3678 100.0 4852.7046 4850.675 9266.3 1 6.168 23.3 4 R.FIPSLIQCIADPTEVPETVHLLGATTFVAEVTPATLSIMVPLLSR.G * Jamie_FT_02_itms_23.16992.16992.4 4.3625 0.103 98.2 4854.9766 4850.675 9274.0 1 3.854 17.4 2 R.FIPSLIQCIADPTEVPETVHLLGATTFVAEVTPATLSIMVPLLSR.G * Jamie_FT_01_itms_18.10800.10800.2 2.5668 0.312 100.0 1526.2322 1526.8162 7757.9 1 5.298 61.5 4 K.LVEDPQVIAPFLGK.L * Jamie_FT_03_itms_10.14501.14501.3 2.2285 0.2227 97.1 1860.6244 1861.1455 4643.7 292 4.655 25.0 1 K.IVVEYIAAIGADLIDER.I * Jamie_FT_02_itms_16.12731.12731.2 4.2672 0.3272 100.0 1861.1921 1861.1455 6865.3 1 7.381 68.8 2 K.IVVEYIAAIGADLIDER.I * Jamie_FT_01_itms_13.07797.07797.2 2.2942 0.0531 97.5 1190.5322 1189.3538 7702.8 5 3.631 66.7 1 K.NLTEEVWAVK.D Reverse_YOR215C 1 1 8.1% 185 21229 9.6 U YOR215C SGDID:S000005741, Chr XV from 747282-746725, reverse complement, Uncharacterized ORF, "Hypothetical protein" * Jamie_FT_01_itms_15.08951.08951.2 1.5105 0.1588 94.9 1756.9122 1757.9093 6759.0 183 3.297 32.1 1 K.SYMDYLSYEDASKGK.L YHL001W 1 3 8.0% 138 15153 10.9 U RPL14B SGDID:S000000993, Chr VIII from 104272-104400,104799-105086, Verified ORF, "Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl14Ap and has similarity to rat L14 ribosomal protein" YKL006W 1 3 8.0% 138 15167 10.9 U RPL14A SGDID:S000001489, Chr XI from 431549-431677,432076-432363, Verified ORF, "N-terminally acetylated protein component of the large (60S) ribosomal subunit, nearly identical to Rpl14Bp and has similarity to rat L14 ribosomal protein; rpl14a csh5 double null mutant exhibits synthetic slow growth" Jamie_FT_01_itms_16.09416.09416.2 3.2752 0.3544 100.0 1214.0521 1213.46 7431.5 2 6.456 75.0 3 K.LAAIVEIIDQK.K YFL002C 1 1 7.9% 606 69422 9.0 U SPB4 SGDID:S000001894, Chr VI from 146929-145109, reverse complement, Verified ORF, "Putative ATP-dependent RNA helicase, nucleolar protein required for synthesis of 60S ribosomal subunits at a late step in the pathway; sediments with 66S pre-ribosomes in sucrose gradients" * Jamie_FT_01_itms_31.18601.18601.4 3.8031 0.0717 94.7 5887.3765 5886.7 10965.3 42 2.589 14.5 1 K.QEGIFPGNWLVDPPVNMDEYKY*KDKKREKERQETLKNISLINDKKKLK.S Reverse_YJR015W 1 1 7.6% 510 58117 7.1 U YJR015W SGDID:S000003776, Chr X from 462635-464167, Uncharacterized ORF, "Hypothetical protein" * Jamie_FT_02_itms_30.20754.20754.4 2.8774 0.1426 95.2 4283.177 4283.168 8974.3 140 3.827 16.7 1 K.AAVLAKQKLKRSAYKGVFPLLATNLAILAVLIGY*NRGMK.R YPR191W 1 1 7.6% 368 40478 8.0 U QCR2 SGDID:S000006395, Chr XVI from 919377-920483, Verified ORF, "Subunit 2 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; transcription is regulated by Hap1p, Hap2p/Hap3p, and heme" * Jamie_FT_01_itms_41.24600.24600.2 3.5092 0.3696 100.0 2902.7922 2901.2837 10338.3 1 6.866 37.0 1 K.ASLAQYEVLANYLTSALSELSGLISSAK.L YGR118W 1 6 7.6% 145 16038 10.7 U RPS23A SGDID:S000003350, Chr VII from 726978-727042,727363-727735, Verified ORF, "Ribosomal protein 28 (rp28) of the small (40S) ribosomal subunit, required for translational accuracy; nearly identical to Rps23Bp and similar to E. coli S12 and rat S23 ribosomal proteins; deletion of both RPS23A and RPS23B is lethal" YPR132W 1 6 7.6% 145 16038 10.7 U RPS23B SGDID:S000006336, Chr XVI from 794961-795025,795391-795763, Verified ORF, "Ribosomal protein 28 (rp28) of the small (40S) ribosomal subunit, required for translational accuracy; nearly identical to Rps23Ap and similar to E. coli S12 and rat S23 ribosomal proteins; deletion of both RPS23A and RPS23B is lethal" Jamie_FT_01_itms_22.12636.12636.2 3.7923 0.2882 100.0 1173.5322 1173.441 8157.1 1 6.826 90.0 6 K.VSGVSLLALWK.E YER131W 1 1 7.6% 119 13447 10.9 U RPS26B SGDID:S000000933, Chr V from 423948-424307, Verified ORF, "Protein component of the small (40S) ribosomal subunit; nearly identical to Rps26Ap and has similarity to rat S26 ribosomal protein" YGL189C 1 1 7.6% 119 13505 10.8 U RPS26A SGDID:S000003157, Chr VII from 148594-148235, reverse complement, Verified ORF, "Protein component of the small (40S) ribosomal subunit; nearly identical to Rps26Bp and has similarity to rat S26 ribosomal protein" Jamie_FT_01_itms_9.05153.05153.2 2.0631 0.1552 99.5 943.3522 943.091 5681.8 55 4.043 68.8 1 R.NIVEAAAVR.D Reverse_YDR524C 1 1 7.5% 482 54494 9.5 U AGE1 SGDID:S000002932, Chr IV from 1488980-1487532, reverse complement, Verified ORF, "ADP-ribosylation factor (ARF) GTPase activating protein (GAP) effector, involved in the secretory and endocytic pathways; contains C2C2H2 cysteine/histidine motif" * Jamie_FT_01_itms_29.16915.16915.4 3.4262 0.0705 90.8 4164.9365 4165.662 7783.7 55 3.389 19.5 1 K.LLINTLKLANNCWSPY*QAKSATRAVTGKS*FLPVVNK.N YMR203W 1 3 7.5% 387 42038 5.5 U TOM40 SGDID:S000004816, Chr XIII from 668491-669654, Verified ORF, "Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore" * Jamie_FT_01_itms_35.20766.20766.2 4.3612 0.3958 100.0 3056.7322 3056.4858 7145.2 1 7.98 35.7 3 K.GEFTGVAVASFLQSVTPQLALGLETLYSR.T YPR183W 1 1 7.5% 267 30362 7.9 U DPM1 SGDID:S000006387, Chr XVI from 900751-901554, Verified ORF, "Dolichol phosphate mannose (Dol-P-Man) synthase of the ER membrane, catalyzes the formation of Dol-P-Man from Dol-P and GDP-Man; required for glycosyl phosphatidylinositol membrane anchoring, O mannosylation, and protein glycosylation" * Jamie_FT_02_itms_34.23141.23141.2 2.221 0.0536 94.3 2382.1921 2379.5696 10459.5 17 3.667 36.8 1 K.GQY*LVCMDADLQHPPETVPK.L YHR064C 1 1 7.4% 538 58238 5.1 U SSZ1 SGDID:S000001106, Chr VIII from 227143-225527, reverse complement, Verified ORF, "Hsp70 protein that interacts with Zuo1p (a DnaJ homolog) to form a ribosome-associated complex that is bound to the ribosome via the Zuo1p subunit; also involved in pleiotropic drug resistance via sequential activation of PDR1 and PDR5" * Jamie_FT_01_itms_31.18384.18384.3 4.3458 0.2651 100.0 4358.3345 4354.9956 9902.3 1 5.735 21.2 1 K.IGLQIVQFINEPSAALLAHAEQFPFEKDVNVVVADFGGIR.S YBR161W 1 1 7.4% 376 44382 9.1 U CSH1 SGDID:S000000365, Chr II from 561629-562759, Verified ORF, "Probable catalytic subunit of a mannosylinositol phosphorylceramide (MIPC) synthase, forms a complex with probable regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Sur1p" * Jamie_FT_01_itms_33.19409.19409.2 2.1494 0.0962 97.3 3213.2322 3214.426 7152.6 11 3.136 22.2 1 K.DALTDEQLNPPNGFNSTFY*ESPPQLIPK.I Reverse_YCL049C 1 1 7.4% 312 35652 4.8 U YCL049C SGDID:S000000554, Chr III from 40724-39786, reverse complement, Uncharacterized ORF, "Hypothetical protein" * Jamie_FT_03_itms_19.20224.20224.2 1.563 0.1395 94.0 2728.4321 2726.225 8907.8 39 3.987 29.5 1 R.IMPAVMISSQS*VTQNIKKLWNKK.D Reverse_YOR179C 1 1 7.4% 188 20866 5.6 U SYC1 SGDID:S000005705, Chr XV from 672411-671845, reverse complement, Verified ORF, "Subunit of the APT subcomplex of cleavage and polyadenylation factor, may have a role in 3' end formation of both polyadenylated and non-polyadenylated RNAs" * Jamie_FT_02_itms_8.07937.07937.2 1.8019 0.0898 93.5 1539.5322 1541.6584 8504.8 58 3.107 46.2 1 K.SKGIT*VSGNKVNEK.K Reverse_YEL057C 1 1 7.3% 233 27229 9.7 U YEL057C SGDID:S000000783, Chr V from 45721-45020, reverse complement, Uncharacterized ORF, "Hypothetical protein" * Jamie_FT_02_itms_11.09921.09921.2 1.7331 0.0903 92.3 2096.2922 2099.2273 11857.8 16 2.895 37.5 1 K.SYASFQVT*KFGKRNDNR.Q YBR127C 2 3 7.2% 517 57749 5.1 U VMA2 SGDID:S000000331, Chr II from 492816-491263, reverse complement, Verified ORF, "Subunit B of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; contains nucleotide binding sites; also detected in the cytoplasm" * Jamie_FT_01_itms_19.11148.11148.2 2.6997 0.3136 100.0 1929.2122 1930.1389 7586.5 2 6.86 50.0 1 R.GYPGYMYTDLSTIYER.A * Jamie_FT_02_itms_12.10259.10259.3 3.4261 0.275 100.0 2321.3943 2320.642 6315.9 1 5.21 36.2 2 K.AVVGEEALSIEDKLSLEFLEK.F Reverse_YBR165W 1 1 7.2% 277 32197 8.6 U UBS1 SGDID:S000000369, Chr II from 568847-569680, Verified ORF, "Ubiquitin-conjugating enzyme suppressor that functions as a general positive regulator of Cdc34p activity; nuclear protein that may represent a link between nucleocytoplasmic transport and ubiquitin ligase activity" * Jamie_FT_01_itms_40.24000.24000.3 2.6089 0.2408 99.0 2527.2544 2525.4443 4081.0 107 4.58 30.3 1 R.KT*YHESGRNDNT*EDPCALTR.S YOL003C 1 1 7.1% 378 44488 8.3 U YOL003C SGDID:S000005363, Chr XV from 322994-321858, reverse complement, Verified ORF, "Palmitoyltransferase with autoacylation activity; member of a family of putative palmitoyltransferases containing an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain" * Jamie_FT_02_itms_18.13775.13775.3 2.9432 0.1655 96.7 3241.5842 3240.765 7926.0 149 3.782 21.2 1 K.KSELIFLTISSPLNS*FVLLT*ITILFLR.C YAL012W 1 1 7.1% 394 42542 6.5 U CYS3 SGDID:S000000010, Chr I from 130802-131986, Verified ORF, "Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine;" * Jamie_FT_01_itms_32.18829.18829.3 4.2533 0.3351 100.0 2882.7244 2881.4004 6302.4 40 5.795 23.1 1 R.LFTLAESLGGIESLLEVPAVMTHGGIPK.E YNL112W 1 1 7.0% 546 60999 8.8 U DBP2 SGDID:S000005056, Chr XIV from 413641-414913,415916-416283, Verified ORF, "Essential ATP-dependent RNA helicase of the DEAD-box protein family, involved in nonsense-mediated mRNA decay and rRNA processing" * Jamie_FT_01_itms_35.20628.20628.3 3.9695 0.3109 100.0 4176.474 4172.6353 8396.9 4 5.002 18.2 1 K.QLAADYLNDPIQVQVGSLELSASHNITQIVEVVSDFEK.R YIL097W 1 1 7.0% 516 59894 7.1 U FYV10 SGDID:S000001359, Chr IX from 180424-181974, Verified ORF, "Protein of unknown function, required for survival upon exposure to K1 killer toxin; involved in proteasome-dependent catabolite inactivation of fructose-1,6-bisphosphatase; contains CTLH domain" * Jamie_FT_02_itms_23.17003.17003.4 3.4724 0.0645 90.3 4344.0967 4343.907 10635.8 202 3.013 17.1 1 K.SQSLPMKKDRIFQHFFHKS*LPRITSKPAVNTTDYDK.S YNL301C 1 1 7.0% 186 20563 11.7 U RPL18B SGDID:S000005245, Chr XIV from 64562-64451,64018-63570, reverse complement, Verified ORF, "Protein component of the large (60S) ribosomal subunit, identical to Rpl18Ap and has similarity to rat L18 ribosomal protein" YOL120C 1 1 7.0% 186 20563 11.7 U RPL18A SGDID:S000005480, Chr XV from 94401-94290,93842-93394, reverse complement, Verified ORF, "Protein component of the large (60S) ribosomal subunit, identical to Rpl18Bp and has similarity to rat L18 ribosomal protein; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation" Jamie_FT_01_itms_10.05520.05520.2 3.7648 0.45 100.0 1331.8922 1332.4967 8348.7 1 8.58 75.0 1 K.TVVVVGTVTDDAR.I YDR481C 1 1 6.9% 566 63004 5.6 U PHO8 SGDID:S000002889, Chr IV from 1420240-1418540, reverse complement, Verified ORF, "Repressible alkaline phosphatase, a glycoprotein localized to the vacuole; regulated by levels of inorganic phosphate and by a system consisting of Pho4p, Pho9p, Pho80p, Pho81p and Pho85p; dephosphorylates phosphotyrosyl peptides" * Jamie_FT_01_itms_39.22997.22997.3 4.1562 0.1974 100.0 4552.074 4549.9976 9340.5 17 4.676 17.8 1 K.ATWSYVLNNLQGNHENTEVGQFLENFLELNLNEVTDLIR.D YMR253C 1 1 6.8% 414 46659 8.8 U YMR253C SGDID:S000004866, Chr XIII from 777189-775945, reverse complement, Uncharacterized ORF, "Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern" * Jamie_FT_03_itms_7.12870.12870.3 2.7225 0.1253 90.8 3346.5842 3346.9182 9692.9 214 3.487 22.2 1 K.KQWILFGNLGVS*GFIFQLLLTMGIQRER.A YJL008C 1 1 6.7% 568 61662 5.7 U CCT8 SGDID:S000003545, Chr X from 421578-419872, reverse complement, Verified ORF, "Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo" * Jamie_FT_01_itms_33.19818.19818.3 5.482 0.3748 100.0 4165.7046 4162.6426 7696.8 1 6.285 24.3 1 K.KYGSEDILSELVSEAVSHVLPVAQQAGEIPYFNVDSIR.V YNL124W 1 1 6.7% 492 54949 4.8 U NAF1 SGDID:S000005068, Chr XIV from 392894-394372, Verified ORF, "Protein required for the assembly of box H/ACA snoRNPs and for pre-rRNA processing, forms a complex with Shq1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; has similarity to Gar1p and other RNA-binding proteins" * Jamie_FT_01_itms_40.23611.23611.4 4.3991 0.302 100.0 3809.5366 3809.2373 6630.1 400 5.597 18.2 1 D.YEIS*EKTIITPIGVLKSAFENNIIIHATMS*GEK.R YDR372C 1 1 6.7% 345 39287 5.1 U VPS74 SGDID:S000002780, Chr IV from 1222139-1221102, reverse complement, Verified ORF, "Non-essential protein of unknown function involved in vacuolar protein sorting; belongs to a family of cytosolic Golgi-associated proteins suggesting that it may play a role in secretion; also detected in the nucleus" * Jamie_FT_01_itms_38.22331.22331.2 4.0583 0.4253 100.0 2659.912 2658.9678 7509.2 1 6.969 43.2 1 K.NDEPLSISNWIDLLSGETWNLLK.I YER074W 1 4 6.7% 135 15329 10.5 U RPS24A SGDID:S000000876, Chr V from 306319-306321,306788-307192, Verified ORF, "Protein component of the small (40S) ribosomal subunit; identical to Rps24Bp and has similarity to rat S24 ribosomal protein" YIL069C 1 4 6.7% 135 15329 10.5 U RPS24B SGDID:S000001331, Chr IX from 232366-232364,231954-231550, reverse complement, Verified ORF, "Protein component of the small (40S) ribosomal subunit; identical to Rps24Ap and has similarity to rat S24 ribosomal protein" Jamie_FT_01_itms_16.09255.09255.2 2.5483 0.252 100.0 999.3522 998.12646 7012.8 1 5.707 81.2 4 K.DAVSVFGFR.T YIL005W 1 1 6.6% 701 81219 7.8 U EPS1 SGDID:S000001267, Chr IX from 345689-347794, Verified ORF, "Pdi1p (protein disulfide isomerase)-related protein involved in endoplasmic reticulum retention of resident ER proteins" * Jamie_FT_01_itms_36.21542.21542.4 3.5025 0.0975 95.6 5614.4165 5618.4023 10184.5 48 3.592 14.8 1 K.APANKIVDKMREEIPHMDQKKLLLGYLDISKEKNFFRKY*GITGEY*K.I YBL039C 2 2 6.6% 579 64710 6.0 U URA7 SGDID:S000000135, Chr II from 145731-143992, reverse complement, Verified ORF, "Major CTP synthase isozyme (see also URA8), catalyzes the ATP-dependent transfer of the amide nitrogen from glutamine to UTP, forming CTP, the final step in de novo biosynthesis of pyrimidines; involved in phospholipid biosynthesis" * Jamie_FT_01_itms_36.21530.21530.3 2.9724 0.1588 96.2 2638.7944 2639.1077 7140.6 130 4.197 22.0 1 K.VLDPSKPFLGLVAASAGILQDVIEGK.Y * Jamie_FT_01_itms_38.22266.22266.3 6.2463 0.34 100.0 4066.3743 4067.6282 6599.2 1 6.552 25.0 1 K.VLDPSKPFLGLVAASAGILQDVIEGKYDLEAGENKFNF.- YJR007W 1 1 6.6% 304 34718 5.0 U SUI2 SGDID:S000003767, Chr X from 450934-451848, Verified ORF, "Alpha subunit of the translation initiation factor eIF2, involved in the identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP" * Jamie_FT_02_itms_24.17547.17547.2 1.7355 0.0852 91.5 2279.3123 2278.7935 10341.7 168 3.153 26.3 1 R.IRSIQKLIRVGKNDVAVVLR.V Reverse_YFL044C 1 1 6.6% 301 33510 5.3 U YFL044C SGDID:S000001850, Chr VI from 45560-44655, reverse complement, Uncharacterized ORF, "Protein of unknown function, proposed to act as a deubiquitinating (DUB) enzyme and/or transcription factor; contains a C2H2-type DNA-binding Zn finger domain; has similarity to the ovarian tumor (OTU) superfamily of cysteine proteases" * Jamie_FT_03_itms_8.13766.13766.2 2.0643 0.1061 97.2 2205.7122 2208.344 10608.8 26 3.091 34.2 1 K.LNSALQLAAT*LVDDSEPQNK.N YOL080C 1 1 6.6% 289 32895 9.8 U REX4 SGDID:S000005440, Chr XV from 181426-180557, reverse complement, Verified ORF, "Putative RNA exonuclease possibly involved in pre-rRNA processing and ribosome assembly" * Jamie_FT_03_itms_4.10876.10876.2 1.6562 0.1471 96.0 2231.7922 2233.6182 5499.4 3 4.577 38.9 1 R.ISIVNYFGHVVLDEFVKPR.E YMR102C 1 1 6.5% 834 94254 8.7 U YMR102C SGDID:S000004708, Chr XIII from 472351-469847, reverse complement, Uncharacterized ORF, "Protein of unknown function, transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance" * Jamie_FT_02_itms_18.14036.14036.4 3.1264 0.1009 93.6 6362.9766 6359.0464 10845.7 297 3.643 12.3 1 K.RKPKGLARSGS*LRSIFSKSMSRSSSQNNEEKPHHHLKLTNLLPLPHHSNDHY*IK.N YMR179W 1 1 6.3% 758 84697 6.0 U SPT21 SGDID:S000004791, Chr XIII from 619857-622133, Verified ORF, "Protein required for normal transcription at several loci including HTA2-HTB2 and HHF2-HHT2, but not required at the other histone loci; functionally related to Spt10p" * Jamie_FT_01_itms_35.20858.20858.4 3.5648 0.0608 90.8 5494.4565 5493.8076 8399.5 271 3.063 13.8 1 K.NS*AKSTQAGCRRSSVIEHLNDHDDSILSDILS*EPGIEGQKLQQKQKGR.K YBR117C 1 1 6.3% 681 75029 6.1 U TKL2 SGDID:S000000321, Chr II from 476431-474386, reverse complement, Verified ORF, "Transketolase, similar to Tkl1p; catalyzes conversion of xylulose-5-phosphate and ribose-5-phosphate to sedoheptulose-7-phosphate and glyceraldehyde-3-phosphate in the pentose phosphate pathway; needed for synthesis of aromatic amino acids" * Jamie_FT_01_itms_36.21422.21422.3 3.1859 0.1142 94.7 5036.7544 5039.399 9265.3 237 3.343 13.7 1 K.YAHQS*FGLDEFGRS*GKGPEIYKLFDFTADGVASRAEKTINYYK.G YGR124W 1 1 6.3% 572 64593 5.9 U ASN2 SGDID:S000003356, Chr VII from 739949-741667, Verified ORF, "Asparagine synthetase, isozyme of Asn1p; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway" YPR145W 1 1 6.3% 572 64470 6.1 U ASN1 SGDID:S000006349, Chr XVI from 822616-824334, Verified ORF, "Asparagine synthetase, isozyme of Asn2p; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway" Jamie_FT_01_itms_30.17623.17623.4 4.4029 0.195 100.0 4203.3364 4201.6387 9700.5 8 3.942 20.0 1 K.FIGSIHHEHTFTLQEGLDALDDVIYHLETYDVTTIR.A YKL133C 1 1 6.3% 463 54438 9.1 U YKL133C SGDID:S000001616, Chr XI from 193069-191678, reverse complement, Uncharacterized ORF, "Hypothetical protein" * Jamie_FT_05_itms_2.12951.12951.3 2.4032 0.1575 91.4 3346.7344 3347.8853 9185.1 87 4.029 19.6 1 K.NVLSKSELLNNQQLDWKNFKGLPFIGKSK.P YML085C 1 2 6.3% 447 49800 5.1 U TUB1 SGDID:S000004550, Chr XIII from 99400-99376,99259-97941, reverse complement, Verified ORF, "Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules" * Jamie_FT_01_itms_38.22721.22721.3 4.496 0.4296 100.0 3011.2444 3011.4514 10320.8 1 7.025 25.9 2 R.NLDIPRPSFANLNNLIAQVVSSVTASLR.F YOR201C 2 5 6.3% 412 46387 9.2 U YOR201C SGDID:S000005727, Chr XV from 721708-720470, reverse complement, Verified ORF, "Ribose methyltransferase that modifies a functionally critical, conserved nucleotide in mitochondrial 21S rRNA" * Jamie_FT_02_itms_23.16891.16891.3 3.5878 0.2632 100.0 2767.3442 2768.1865 6347.7 3 5.262 27.0 2 R.APEPIVDSLNVSVATALLIDNILTCK.- * Jamie_FT_01_itms_36.21086.21086.2 5.0681 0.4533 100.0 2769.8323 2768.1865 9128.6 1 8.13 50.0 3 R.APEPIVDSLNVSVATALLIDNILTCK.- YBR002C 1 1 6.3% 286 32694 7.8 U RER2 SGDID:S000000206, Chr II from 242570-241710, reverse complement, Verified ORF, "Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting" * Jamie_FT_01_itms_25.14646.14646.2 2.0172 0.0427 90.8 2200.0923 2198.3008 8410.0 10 3.748 35.3 1 K.KEMDVKEGHEAGFVS*MS*R.I YGL147C 1 1 6.3% 191 21569 9.7 U RPL9A SGDID:S000003115, Chr VII from 228334-227759, reverse complement, Verified ORF, "Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl9Bp and has similarity to E. coli L6 and rat L9 ribosomal proteins" YNL067W 1 1 6.3% 191 21657 9.7 U RPL9B SGDID:S000005011, Chr XIV from 499682-500257, Verified ORF, "Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl9Ap and has similarity to E. coli L6 and rat L9 ribosomal proteins" Jamie_FT_01_itms_15.08964.08964.2 2.101 0.1201 98.2 1278.4521 1278.5066 7570.0 19 3.867 59.1 1 K.SLVDNMITGVTK.G Reverse_YDR099W 1 1 6.2% 273 31061 4.9 U BMH2 SGDID:S000002506, Chr IV from 653602-654423, Verified ORF, "14-3-3 protein, minor isoform; binds proteins and DNA, involved in regulation of many processes including exocytosis and vesicle transport, Ras/MAPK signaling during pseudohyphal development, rapamycin-sensitive signaling, and others" Reverse_YER177W 1 1 6.4% 267 30091 4.9 U BMH1 SGDID:S000000979, Chr V from 545606-546409, Verified ORF, "14-3-3 protein, major isoform; binds proteins and DNA, involved in regulation of many processes including exocytosis and vesicle transport, Ras/MAPK signaling during pseudohyphal development, rapamycin-sensitive signaling, and others" Jamie_FT_01_itms_20.11450.11450.2 1.7419 0.1293 95.6 1886.5322 1887.0538 10348.5 2 4.472 46.9 1 R.IPHTPPLETTAIESAT*K.Y Reverse_YLL023C 1 1 6.1% 279 32186 10.0 U YLL023C SGDID:S000003946, Chr XII from 98835-97996, reverse complement, Uncharacterized ORF, "Hypothetical protein" * Jamie_FT_01_itms_13.07418.07418.4 2.9921 0.0811 91.6 2220.4966 2215.4912 6611.5 68 3.624 31.2 1 R.WQAFAAKMYKS*NEYRLK.I YNL069C 1 8 6.1% 198 22249 10.5 U RPL16B SGDID:S000005013, Chr XIV from 495002-494975,494525-493957, reverse complement, Verified ORF, "N-terminally acetylated protein component of the large (60S) ribosomal subunit, binds to 5.8 S rRNA; has similarity to Rpl16Ap, E. coli L13 and rat L13a ribosomal proteins; transcriptionally regulated by Rap1p" * Jamie_FT_01_itms_16.09299.09299.2 3.3099 0.2697 100.0 1356.0322 1354.5052 7585.6 1 5.339 72.7 8 R.AEALNISGEFFR.N YGL135W 1 3 6.0% 217 24486 9.7 U RPL1B SGDID:S000003103, Chr VII from 254646-255299, Verified ORF, "N-terminally acetylated protein component of the large (60S) ribosomal subunit, nearly identical to Rpl1Bp and has similarity to E. coli L1 and rat L10a ribosomal proteins; rpl1a rpl1b double null mutation is lethal" YPL220W 1 3 6.0% 217 24486 9.7 U RPL1A SGDID:S000006141, Chr XVI from 135789-136442, Verified ORF, "N-terminally acetylated protein component of the large (60S) ribosomal subunit, nearly identical to Rpl1Bp and has similarity to E. coli L1 and rat L10a ribosomal proteins; rpl1a rpl1b double null mutation is lethal" Jamie_FT_01_itms_23.13322.13322.2 4.5032 0.3604 100.0 1492.6921 1490.7399 10122.8 1 6.767 83.3 3 R.NFLETVELQVGLK.N YIL133C 1 3 6.0% 199 22201 10.5 U RPL16A SGDID:S000001395, Chr IX from 99416-99386,99095-98527, reverse complement, Verified ORF, "N-terminally acetylated protein component of the large (60S) ribosomal subunit, binds to 5.8 S rRNA; has similarity to Rpl16Bp, E. coli L13 and rat L13a ribosomal proteins; transcriptionally regulated by Rap1p" * Jamie_FT_01_itms_17.10195.10195.2 2.1892 0.0571 95.7 1411.8922 1412.5419 6951.9 14 3.514 63.6 3 R.AEELNISGEFFR.N YLR090W 1 1 5.9% 459 51347 6.2 U XDJ1 SGDID:S000004080, Chr XII from 320702-322081, Verified ORF, "Putative chaperone, homolog of E. coli DnaJ, closely related to Ydj1p" * Jamie_FT_01_itms_35.20487.20487.2 2.1089 0.0394 92.3 2895.0522 2895.1028 10693.6 12 2.885 25.0 1 K.NFDDGAGEDLYTSITISLSEALTGFEK.F Reverse_YGL105W 1 1 5.9% 376 42084 7.9 U ARC1 SGDID:S000003073, Chr VII from 307440-308570, Verified ORF, "Protein that binds tRNA and methionyl- and glutamyl-tRNA synthetases (Mes1p and Gus1p), delivering tRNA to them, stimulating catalysis, and ensuring their localization to the cytoplasm; also binds quadruplex nucleic acids" * Jamie_FT_01_itms_26.15167.15167.2 1.8583 0.0648 91.2 2390.0122 2389.7634 7403.6 59 3.164 28.6 1 -.RVQANAISAVKFSEGKANTLKR.V Reverse_YCL050C 1 1 5.9% 321 36493 5.0 U APA1 SGDID:S000000555, Chr III from 38801-37836, reverse complement, Verified ORF, "Diadenosine 5',5''-P1,P4-tetraphosphate phosphorylase I (AP4A phosphorylase), involved in catabolism of bis(5'-nucleosidyl) tetraphosphates; has similarity to Apa2p" * Jamie_FT_02_itms_26.18835.18835.2 1.6193 0.1054 91.9 2287.392 2286.46 10120.5 71 3.385 30.6 1 K.TTETQIFKLNGNDFAS*KYK.D Reverse_YKL064W 1 1 5.7% 969 109736 7.5 U MNR2 SGDID:S000001547, Chr XI from 317408-320317, Verified ORF, "Putative magnesium transporter; has similarity to Alr1p and Alr2p, which mediate influx of Mg2+ and other divalent cations" * Jamie_FT_02_itms_37.25379.25379.4 2.1776 0.1932 93.7 6604.5366 6603.178 10631.0 53 3.216 12.7 1 K.TMDINIQALYNSHSRSLLKEYHNLSSVMT*VIHDQIDGLY*MAIESQPSAEWQENYR.K YJR121W 2 7 5.7% 511 54794 5.7 U ATP2 SGDID:S000003882, Chr X from 647522-649057, Verified ORF, "Beta subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis" * Jamie_FT_01_itms_36.21564.21564.2 3.1357 0.2911 100.0 3359.5122 3359.8035 6027.6 4 4.876 25.0 1 R.VALTGLTIAEYFRDEEGQDVLLFIDNIFR.F * Jamie_FT_04_itms_13.18037.18037.2 4.2959 0.4061 100.0 1922.9722 1924.1174 8298.8 1 6.59 63.3 6 R.DEEGQDVLLFIDNIFR.F YLR448W 1 1 5.7% 176 19986 10.1 U RPL6B SGDID:S000004440, Chr XII from 1028849-1028863,1029248-1029763, Verified ORF, "Protein component of the large (60S) ribosomal subunit, has similarity to Rpl6Bp and to rat L6 ribosomal protein; binds to 5.8S rRNA" YML073C 1 1 5.7% 176 19961 10.1 U RPL6A SGDID:S000004538, Chr XIII from 124172-124158,123742-123227, reverse complement, Verified ORF, "N-terminally acetylated protein component of the large (60S) ribosomal subunit, has similarity to Rpl6Bp and to rat L6 ribosomal protein; binds to 5.8S rRNA" Jamie_FT_01_itms_9.05250.05250.2 2.0437 0.1662 99.5 1059.0322 1060.1925 7002.5 10 4.401 72.2 1 K.VSVEGVNVEK.F Reverse_YPL089C 1 1 5.6% 676 73484 9.3 U RLM1 SGDID:S000006010, Chr XVI from 381147-379117, reverse complement, Verified ORF, "MADS-box transcription factor, component of the protein kinase C-mediated MAP kinase pathway involved in the maintenance of cell integrity; phosphorylated and activated by the MAP-kinase Slt2p" * Jamie_FT_01_itms_35.20600.20600.4 3.5834 0.0966 95.6 4435.9766 4433.724 8546.8 226 3.494 16.2 1 K.SPKNSMS*SRLLDQNINVS*ASKHFDGYDSPDKVEHLLNK.D YER062C 1 1 5.6% 250 27814 6.2 U HOR2 SGDID:S000000864, Chr V from 280680-279928, reverse complement, Verified ORF, "One of two redundant DL-glycerol-3-phosphatases (RHR2/GPP1 encodes the other) involved in glycerol biosynthesis; induced in response to hyperosmotic stress and oxidative stress, and during the diauxic transition" * Jamie_FT_01_itms_12.07044.07044.4 3.1272 0.0736 94.4 1726.5765 1727.8657 2553.3 289 3.155 38.5 1 K.IIGIAT*T*FDLDFLK.E YBL057C 1 1 5.6% 214 23128 5.4 U PTH2 SGDID:S000000153, Chr II from 113447-112803, reverse complement, Verified ORF, "One of two (see also PTH1) mitochondrially-localized peptidyl-tRNA hydrolases; dispensable for cell growth" * Jamie_FT_01_itms_12.07033.07033.2 2.5469 0.0655 97.9 1431.3922 1431.6042 6789.0 9 3.885 59.1 1 K.SSAT*LLRSKEMK.E YGR145W 1 1 5.5% 707 81748 6.6 U ENP2 SGDID:S000003377, Chr VII from 781772-783895, Uncharacterized ORF, "Essential nucleolar protein of unknown function; contains WD repeats, interacts with Mpp10p and Bfr2p, and has homology to Spb1p" * Jamie_FT_02_itms_11.09786.09786.4 3.586 0.0824 94.6 4506.2563 4504.827 8359.8 1 3.31 18.9 1 R.FLNPFKLDT*EGVNHVS*INEVNGLLAAGTETNVVEFWDPR.S YGL026C 1 1 5.5% 707 76626 6.5 U TRP5 SGDID:S000002994, Chr VII from 448540-446417, reverse complement, Verified ORF, "Tryptophan synthase involved in tryptophan biosynthesis, regulated by the general control system of amino acid biosynthesis" * Jamie_FT_02_itms_23.16854.16854.4 4.099 0.2644 100.0 4418.2163 4412.8296 8819.2 309 4.712 17.5 1 R.LELLSHIADSFVYVVSRMGTTGVQSSVAS*DLDELIS*RVR.K Reverse_YDR034C-C 1 1 5.5% 438 49689 7.7 U YDR034C-C SGDID:S000007344, Chr IV from 519352-518036, reverse complement, transposable_element_gene, "TyA Gag protein; the main structural constituent of virus-like particles (VLPs)" Reverse_YLR410W-B 1 1 1.4% 1770 202121 8.2 U YLR410W-B SGDID:S000007380, Chr XII from 941480-942772,942774-946793, transposable_element_gene, "TyB Gag-Pol protein; proteolytically processed to make the Gag, RT, PR, and IN proteins that are required for retrotransposition" Reverse_YLR410W-A 1 1 5.5% 438 49771 7.7 U YLR410W-A SGDID:S000007379, Chr XII from 941480-942796, transposable_element_gene, "TyA Gag protein; the main structural constituent of virus-like particles (VLPs)" Reverse_YDR034C-D 1 1 1.4% 1770 201964 8.2 U YDR034C-D SGDID:S000007345, Chr IV from 519352-518060,518058-514039, reverse complement, transposable_element_gene, "TyB Gag-Pol protein; proteolytically processed to make the Gag, RT, PR, and IN proteins that are required for retrotransposition" Jamie_FT_01_itms_31.18113.18113.3 2.8128 0.1139 90.2 2863.2244 2860.0232 7112.1 84 3.351 23.9 1 K.VWTSFNEESTLT*HPPLVNNGVKT*K.A YDL082W 1 1 5.5% 199 22554 11.2 U RPL13A SGDID:S000002240, Chr IV from 308424-308427,308793-309388, Verified ORF, "Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl13Bp; not essential for viability; has similarity to rat L13 ribosomal protein" YMR142C 1 1 5.5% 199 22525 11.1 U RPL13B SGDID:S000004750, Chr XIII from 551206-551203,550800-550205, reverse complement, Verified ORF, "Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl13Ap; not essential for viability; has similarity to rat L13 ribosomal protein" Jamie_FT_01_itms_10.05503.05503.2 3.1319 0.2697 100.0 1335.5721 1334.4313 7075.7 12 4.879 70.0 1 R.NQEIFDANVQR.L YHR019C 2 2 5.4% 554 62207 5.8 U DED81 SGDID:S000001061, Chr VIII from 143551-141887, reverse complement, Verified ORF, "Cytosolic asparaginyl-tRNA synthetase, required for protein synthesis, catalyzes the specific attachment of asparagine to its cognate tRNA" * Jamie_FT_01_itms_39.23235.23235.4 4.8293 0.3488 100.0 3542.1765 3541.038 10051.1 1 5.528 27.6 1 R.HLSEYTHIEAELAFLTFDDLLQHIETLIVK.S * Jamie_FT_01_itms_39.23112.23112.3 4.5851 0.3109 100.0 3545.4543 3541.038 9938.9 1 5.656 27.6 1 R.HLSEYTHIEAELAFLTFDDLLQHIETLIVK.S YPR036W 1 1 5.4% 478 54416 6.4 U VMA13 SGDID:S000006240, Chr XVI from 643833-645269, Verified ORF, "Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase" * Jamie_FT_01_itms_38.22260.22260.2 3.9326 0.3221 100.0 2902.2522 2901.4136 6842.4 1 5.998 36.0 1 K.IIIQVALNDITHVVELLPESIDVLDK.T Reverse_YCR028C 1 1 5.3% 512 58257 7.2 U FEN2 SGDID:S000000623, Chr III from 172419-170881, reverse complement, Verified ORF, "Plasma membrane H+-pantothenate symporter; confers sensitivity to the antifungal agent fenpropimorph" * Jamie_FT_01_itms_41.24201.24201.2 1.5482 0.1319 93.3 3108.912 3106.4004 11448.1 28 3.026 23.1 1 R.PIKSMY*VAS*CLTSVIGVAFIGSPYNNR.Q YBR283C 1 1 5.3% 490 53312 8.1 U SSH1 SGDID:S000000487, Chr II from 770411-768939, reverse complement, Verified ORF, "Subunit of the Ssh1 translocon complex; Sec61p homolog involved in co-translational pathway of protein translocation; not essential" * Jamie_FT_01_itms_49.29378.29378.3 4.2574 0.3463 100.0 2526.2043 2523.072 5191.2 1 6.277 35.0 1 K.VIPIAAVTGASVLSLITVIGESLGLK.G YLR212C 1 2 5.3% 473 52628 4.7 U TUB4 SGDID:S000004202, Chr XII from 566283-564862, reverse complement, Verified ORF, "Gamma-tubulin, involved in nucleating microtubules from both the cytoplasmic and nuclear faces of the spindle pole body" * Jamie_FT_01_itms_31.18324.18324.2 3.915 0.2468 100.0 2781.0122 2780.1106 8470.9 1 5.576 35.4 2 R.NPNIDLQHTNQLISTIISSVTNSIR.F YLR355C 1 1 5.3% 395 44368 9.0 U ILV5 SGDID:S000004347, Chr XII from 839252-838065, reverse complement, Verified ORF, "Acetohydroxyacid reductoisomerase, mitochondrial protein involved in branched-chain amino acid biosynthesis, also required for maintenance of wild-type mitochondrial DNA" * Jamie_FT_01_itms_19.10926.10926.2 2.3871 0.2485 100.0 2245.892 2245.4563 8190.9 13 5.149 35.0 1 K.NDTFALIGYGSQGYGQGLNLR.D YDL198C 1 1 5.3% 300 33215 10.0 U GGC1 SGDID:S000002357, Chr IV from 104552-103650, reverse complement, Verified ORF, "Mitochondrial GTP/GDP transporter, essential for mitochondrial genome maintenance; has a role in mitochondrial iron transport; member of the mitochondrial carrier family; (putative) mitochondrial carrier protein" * Jamie_FT_01_itms_25.14421.14421.2 2.2105 0.158 99.5 1957.4722 1955.3103 10219.1 198 4.179 33.3 1 R.GFIKILRDEGLFNLYR.G Reverse_YLL041C 1 1 5.3% 266 30231 8.8 U SDH2 SGDID:S000003964, Chr XII from 53930-53130, reverse complement, Verified ORF, "Iron-sulfur protein subunit of succinate dehydrogenase (Sdh1p, Sdh2p, Sdh3p, Sdh4p), which couples the oxidation of succinate to the transfer of electrons to ubiquinone" * Jamie_FT_02_itms_41.27558.27558.2 2.3383 0.1005 98.1 1817.6522 1817.9868 9457.1 61 3.615 42.3 1 R.YLSMS*NNLMAKRT*K.T YER001W 1 1 5.2% 762 88529 7.2 U MNN1 SGDID:S000000803, Chr V from 153519-155807, Verified ORF, "Alpha-1,3-mannosyltransferase, integral membrane glycoprotein of the Golgi complex, required for addition of alpha1,3-mannose linkages to N-linked and O-linked oligosaccharides, one of five S. cerevisiae proteins of the MNN1 family" * Jamie_FT_01_itms_27.15978.15978.4 4.5077 0.1632 100.0 4483.5767 4479.8345 9192.5 186 3.533 16.2 1 K.NTAHCTHGDKENFWLGFLAAGHTYALQGVY*SGAIGDYVKK.T YGL236C 1 1 5.2% 669 74215 8.3 U MTO1 SGDID:S000003205, Chr VII from 55796-53787, reverse complement, Verified ORF, "Mitochondrial protein, forms a heterodimer complex with Mss1p that performs the 5-carboxymethylaminomethyl modification of the wobble uridine base in mitochondrial tRNAs; required for respiration in paromomycin-resistant 15S rRNA mutants" * Jamie_FT_04_itms_9.15574.15574.4 2.2569 0.1795 93.5 3772.6965 3772.115 7642.3 392 3.974 18.1 1 K.VIKGVVLDDGT*QVGADQVIITTGTFLS*AEIHIGDK.R YMR296C 1 1 5.2% 558 62207 6.2 U LCB1 SGDID:S000004911, Chr XIII from 860890-859214, reverse complement, Verified ORF, "Component of serine palmitoyltransferase, responsible along with Lcb2p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine" * Jamie_FT_01_itms_32.19136.19136.3 4.3642 0.2542 100.0 3524.9343 3524.9954 8340.2 221 5.831 22.3 1 K.FIEPYEEEEKFLQSIVDHALINYNVLITR.N YGR175C 1 1 5.2% 496 55126 6.5 U ERG1 SGDID:S000003407, Chr VII from 848428-846938, reverse complement, Verified ORF, "Squalene epoxidase, catalyzes the epoxidation of squalene to 2,3-oxidosqualene; plays an essential role in the ergosterol-biosynthesis pathway and is the specific target of the antifungal drug terbinafine" * Jamie_FT_01_itms_35.20712.20712.2 3.1167 0.2798 100.0 2835.9722 2834.1956 10670.4 1 5.739 30.0 1 R.KSYDSVINVLSVALYSLFAADSDNLK.A Reverse_YOR100C 1 1 5.2% 327 34754 9.8 U CRC1 SGDID:S000005626, Chr XV from 514278-513295, reverse complement, Verified ORF, "Mitochondrial inner membrane carnitine transporter, required for carnitine-dependent transport of acetyl-CoA from peroxisomes to mitochondria during fatty acid beta-oxidation" * Jamie_FT_01_itms_30.17491.17491.2 1.7586 0.1192 94.5 1997.6122 1999.231 10569.8 128 3.65 34.4 1 K.YFGKVSNTFLTGKVQT*K.A YGL031C 1 2 5.2% 155 17614 11.3 U RPL24A SGDID:S000002999, Chr VII from 437939-437472, reverse complement, Verified ORF, "Ribosomal protein L30 of the large (60S) ribosomal subunit, nearly identical to Rpl24Bp and has similarity to rat L24 ribosomal protein; not essential for translation but may be required for normal translation rate" YGR148C 1 2 5.2% 155 17547 11.4 U RPL24B SGDID:S000003380, Chr VII from 787784-787317, reverse complement, Verified ORF, "Ribosomal protein L30 of the large (60S) ribosomal subunit, nearly identical to Rpl24Ap and has similarity to rat L24 ribosomal protein; not essential for translation but may be required for normal translation rate" Jamie_FT_01_itms_20.11665.11665.2 2.2668 0.1895 100.0 1006.1722 1006.2358 6583.6 2 5.144 85.7 2 R.IAWTVLFR.K Reverse_YPL103C 1 1 5.1% 468 54368 7.8 U YPL103C SGDID:S000006024, Chr XVI from 359403-357997, reverse complement, Uncharacterized ORF, "The authentic, non-tagged protein was localized to the mitochondria" * Jamie_FT_01_itms_37.22115.22115.2 2.25 0.0311 92.8 3049.5723 3050.2031 9070.9 121 2.715 26.1 1 K.KVQLTEWWSLELVNDCGNSTMY*S*K.M YEL002C 1 1 5.1% 430 49392 5.9 U WBP1 SGDID:S000000728, Chr V from 150013-148721, reverse complement, Verified ORF, "Beta subunit of the oligosaccharyl transferase (OST) glycoprotein complex; required for N-linked glycosylation of proteins in the endoplasmic reticulum" * Jamie_FT_01_itms_27.15786.15786.3 2.6156 0.1813 96.2 2629.0144 2631.645 6736.7 137 3.796 26.2 1 K.SVHAVHS*HADGTS*YDEEPYKIK.D YOR051C 2 2 5.1% 412 47352 4.8 U YOR051C SGDID:S000005577, Chr XV from 426085-424847, reverse complement, Uncharacterized ORF, "Nuclear protein that inhibits replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts" * Jamie_FT_01_itms_39.23097.23097.3 3.8781 0.3128 100.0 2380.2544 2380.7893 7277.7 3 5.262 33.8 1 K.TLNDIFHGIYALALSELTIFK.A * Jamie_FT_01_itms_39.23083.23083.2 4.9731 0.5208 100.0 2380.8523 2380.7893 9151.0 1 8.237 52.5 1 K.TLNDIFHGIYALALSELTIFK.A YLR197W 1 1 5.0% 504 56864 8.9 U SIK1 SGDID:S000004187, Chr XII from 546099-547613, Verified ORF, "Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects" * Jamie_FT_01_itms_30.17959.17959.3 3.8104 0.2497 100.0 2886.4744 2884.2798 7261.3 30 4.393 25.0 1 K.NDNHIIQAIALLDQLDKDINTFAMR.V YDL055C 1 2 5.0% 361 39566 6.3 U PSA1 SGDID:S000002213, Chr IV from 356759-355674, reverse complement, Verified ORF, "GDP-mannose pyrophosphorylase (mannose-1-phosphate guanyltransferase), synthesizes GDP-mannose from GTP and mannose-1-phosphate in cell wall biosynthesis; required for normal cell wall structure" * Jamie_FT_01_itms_16.09041.09041.2 2.6552 0.0554 97.5 1778.6122 1779.0464 6791.9 4 4.719 47.1 2 K.IGPDVVIGPNVTIGDGVR.I YGR086C 1 1 5.0% 339 38349 4.6 U PIL1 SGDID:S000003318, Chr VII from 650621-649602, reverse complement, Verified ORF, "Long chain base-responsive inhibitor of protein kinases Pkh1p and Pkh2p, acts along with Lsp1p to down-regulate heat stress resistance via regulation of the Pkc1p and Ypk1p pathways; phosphorylated by Phk1p and Phk2p" * Jamie_FT_02_itms_30.21169.21169.2 1.7315 0.0963 92.7 1906.5721 1907.0416 8256.7 29 3.258 37.5 1 K.ALLELLDDSPVTPGET*R.P YBR191W 1 1 5.0% 160 18242 10.4 U RPL21A SGDID:S000000395, Chr II from 606265-606275,606664-607135, Verified ORF, "Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl21Bp and has similarity to rat L21 ribosomal protein" YPL079W 1 1 5.0% 160 18274 10.4 U RPL21B SGDID:S000006000, Chr XVI from 406633-406643,407065-407536, Verified ORF, "Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl21Ap and has similarity to rat L21 ribosomal protein" Jamie_FT_01_itms_10.05557.05557.2 2.2044 0.1807 100.0 859.03217 859.01044 6047.7 1 4.448 85.7 1 K.VGDIVDIK.A Reverse_YDR006C 1 1 4.9% 901 101082 9.1 U SOK1 SGDID:S000002413, Chr IV from 461244-458539, reverse complement, Verified ORF, "Protein whose overexpression suppresses the growth defect of mutants lacking protein kinase A activity; involved in cAMP-mediated signaling; localized to the nucleus; similar to the mouse testis-specific protein PBS13" * Jamie_FT_01_itms_35.20652.20652.3 2.9768 0.1089 92.2 4669.344 4668.7393 10159.4 86 3.055 14.5 1 K.LDSSNASNGSSDNNTANT*SANLSMSPT*IVGSSRSLLDSSQDIYK.Q YLL024C 2 5 4.9% 639 69470 5.1 U SSA2 SGDID:S000003947, Chr XII from 97484-95565, reverse complement, Verified ORF, "ATP binding protein involved in protein folding and vacuolar import of proteins; member of heat shock protein 70 (HSP70) family; associated with the chaperonin-containing T-complex; present in the cytoplasm, vacuolar membrane and cell wall" * Jamie_FT_02_itms_17.13526.13526.3 6.401 0.3865 100.0 3365.3342 3365.8047 10439.3 1 8.191 34.2 4 K.GKEEHVLIFDLGGGTFDVSLLSIEDGIFEVK.A * Jamie_FT_02_itms_18.13535.13535.4 4.5131 0.2189 100.0 3366.0566 3365.8047 8650.5 1 4.502 23.9 1 K.GKEEHVLIFDLGGGTFDVSLLSIEDGIFEVK.A YNL041C 1 1 4.8% 839 96976 5.3 U COG6 SGDID:S000004986, Chr XIV from 551988-549469, reverse complement, Verified ORF, "Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments" * Jamie_FT_01_itms_29.16964.16964.4 3.6875 0.0742 94.4 4816.0967 4821.2266 7303.0 40 3.1 16.7 1 R.IKRLS*S*SVEKIQRTSEKLLSNETNEVPTNNVVLQEIDQYR.L YKL049C 1 1 4.8% 229 26841 9.3 U CSE4 SGDID:S000001532, Chr XI from 346408-345719, reverse complement, Verified ORF, "Centromere protein that resembles histones, required for proper kinetochore function; homolog of human CENP-A" * Jamie_FT_01_itms_19.11372.11372.2 1.6599 0.0994 93.3 1485.9922 1484.5199 8336.7 7 3.368 60.0 1 K.Y*QRSTDLLIS*K.I YKL104C 2 2 4.7% 717 80047 6.4 U GFA1 SGDID:S000001587, Chr XI from 245017-242864, reverse complement, Verified ORF, "Glutamine-fructose-6-phosphate amidotransferase, catalyzes the formation of glucosamine-6-P and glutamate from fructose-6-P and glutamine in the first step of chitin biosynthesis" * Jamie_FT_02_itms_12.10167.10167.2 1.9154 0.1487 97.8 1710.4922 1708.0143 8684.6 6 4.179 42.9 1 K.LVLLELEGSYGLLCK.S * Jamie_FT_01_itms_23.13475.13475.2 2.5165 0.0898 98.6 2037.0922 2036.3818 11487.8 30 3.827 38.9 1 K.HGVLALVDENLPIIAFGTR.D Reverse_YDR131C 1 1 4.7% 556 65243 7.1 U YDR131C SGDID:S000002538, Chr IV from 718455-716785, reverse complement, Uncharacterized ORF, "Hypothetical protein" * Jamie_FT_02_itms_41.27386.27386.3 2.9142 0.1893 97.1 3005.0044 3003.311 9363.0 24 4.407 27.0 1 K.NLYSGSVSNHTPMLTPPT*LT*LTKLQK.S YHR007C 2 2 4.7% 530 60720 8.7 U ERG11 SGDID:S000001049, Chr VIII from 121678-120086, reverse complement, Verified ORF, "Lanosterol 14-alpha-demethylase, catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family" * Jamie_FT_01_itms_39.23251.23251.2 2.7006 0.1681 100.0 2710.5322 2712.1643 9839.3 44 4.468 25.0 1 K.SIVGEALEYVNIGLSHFLALPLAQR.I * Jamie_FT_01_itms_39.23341.23341.3 5.3068 0.4894 100.0 2711.6943 2712.1643 9129.4 1 8.681 36.5 1 K.SIVGEALEYVNIGLSHFLALPLAQR.I Reverse_YIR026C 1 1 4.7% 364 41185 8.0 U YVH1 SGDID:S000001465, Chr IX from 405964-404870, reverse complement, Verified ORF, "Protein phosphatase involved in vegetative growth at low temperatures, sporulation, and glycogen accumulation; transcription induced by low temperature and nitrogen starvation; member of the dual-specificity family of protein phosphatases" * Jamie_FT_02_itms_16.12408.12408.2 1.898 0.2289 99.6 2171.8123 2174.267 11510.7 176 4.182 34.4 1 K.S*T*QLHIAPIVWKGCSCR.S Reverse_YNL267W 1 1 4.6% 1066 119923 6.5 U PIK1 SGDID:S000005211, Chr XIV from 140879-144079, Verified ORF, "Phosphatidylinositol 4-kinase; catalyzes first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokineses through the actin cytoskeleton" * Jamie_FT_01_itms_36.21601.21601.4 3.6002 0.0989 95.8 5662.0166 5656.165 7342.0 233 3.378 13.9 1 R.GKPSIYDGKNGDLAKATNS*ISGDRNINKGDYTRHVYSNDLY*KPKRKPLK.I YPL106C 2 2 4.6% 693 77367 5.2 U SSE1 SGDID:S000006027, Chr XVI from 352272-350191, reverse complement, Verified ORF, "ATPase that is a component of the heat shock protein Hsp90 chaperone complex; binds unfolded proteins; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm" Jamie_FT_01_itms_9.05045.05045.2 1.6233 0.0727 91.4 894.6722 893.9749 7282.0 7 3.145 78.6 1 R.YNIADAAR.I * Jamie_FT_03_itms_14.17014.17014.2 1.7792 0.0894 92.7 2633.5723 2635.8938 11128.3 8 3.799 26.1 1 R.IAGLNPVRIVNDVTAAGVS*Y*GIFK.T YHR203C 2 7 4.6% 261 29410 10.1 U RPS4B SGDID:S000001246, Chr VIII from 505530-505517,505247-504476, reverse complement, Verified ORF, "Protein component of the small (40S) ribosomal subunit; identical to Rps4Bp and has similarity to rat S4 ribosomal protein" YJR145C 2 7 4.6% 261 29410 10.1 U RPS4A SGDID:S000003906, Chr X from 702983-702970,702713-701942, reverse complement, Verified ORF, "Protein component of the small (40S) ribosomal subunit; mutation affects 20S pre-rRNA processing; identical to Rps4Bp and has similarity to rat S4 ribosomal protein" Jamie_FT_01_itms_21.12218.12218.3 3.3029 0.0985 97.1 1456.3143 1456.8148 6883.9 62 4.088 50.0 4 K.LRESLPLIVFLR.N Jamie_FT_01_itms_20.11989.11989.2 2.8623 0.2658 100.0 1456.6122 1456.8148 8615.8 14 5.331 54.5 3 K.LRESLPLIVFLR.N Reverse_YPR162C 1 1 4.5% 529 60706 6.8 U ORC4 SGDID:S000006366, Chr XVI from 868300-866711, reverse complement, Verified ORF, "Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing" * Jamie_FT_01_itms_29.17304.17304.2 2.4038 0.1542 99.4 2653.9722 2651.7605 8841.4 98 4.03 26.1 1 K.ITNDISFTSQGT*GVNT*TPAVTPIR.S YDR219C 1 1 4.5% 465 53168 8.7 U YDR219C SGDID:S000002627, Chr IV from 906846-905449, reverse complement, Uncharacterized ORF, "Mitochondria-associated F-box protein involved in maintenance of normal mitochondrial morphology" * Jamie_FT_02_itms_38.25535.25535.2 1.1615 0.2428 96.2 2374.9321 2377.7031 7648.4 80 4.101 20.0 1 K.LGVIDPARKTISYRTNEVESK.E Reverse_YKL141W 1 1 4.5% 198 22068 10.2 U SDH3 SGDID:S000001624, Chr XI from 179672-180268, Verified ORF, "Cytochrome b subunit of succinate dehydrogenase (Sdh1p, Sdh2p, Sdh3p, Sdh4p), which couples the oxidation of succinate to the transfer of electrons to ubiquinone" * Jamie_FT_01_itms_10.05523.05523.2 1.5701 0.1004 92.6 916.5722 919.0286 6601.9 450 3.507 68.8 1 R.RSSSLAAAR.S YJL012C 1 1 4.4% 721 83155 6.8 U VTC4 SGDID:S000003549, Chr X from 413314-411149, reverse complement, Verified ORF, "Vacuolar membrane protein involved in vacuolar polyphosphate accumulation; functions as a regulator of vacuolar H+-ATPase activity and vacuolar transporter chaperones; involved in non-autophagic vacuolar fusion" * Jamie_FT_01_itms_37.21941.21941.3 4.1516 0.3296 100.0 3608.9043 3608.9783 3072.5 1 5.21 25.0 1 R.LLDSNNPPTQLDFEILEEELSDIIADVHDLAK.F Reverse_YDR244W 1 1 4.4% 612 69324 5.0 U PEX5 SGDID:S000002652, Chr IV from 950557-952395, Verified ORF, "Peroxisomal membrane signal receptor for C-terminal tripeptide signal sequence (PTS1) of peroxisomal matrix proteins, required for peroxisomal matrix protein import, tetratricopeptide repeat protein, also involved in PTS1-independent import" * Jamie_FT_01_itms_39.22851.22851.2 2.3345 0.2677 90.6 2994.632 2996.1338 11267.2 148 5.127 23.1 1 K.IQTPNAST*QLSHVNSFPPLRGSS*PGIE.M YFL037W 1 1 4.4% 457 50923 4.7 U TUB2 SGDID:S000001857, Chr VI from 56335-57708, Verified ORF, "Beta-tubulin; associates with alpha-tubulin (Tub1p and Tub3p) to form tubulin dimer, which polymerizes to form microtubules" * Jamie_FT_01_itms_22.13028.13028.3 3.176 0.087 93.8 2243.1543 2243.631 8126.0 59 3.332 30.3 1 R.LHFFMVGYAPLTAIGSQSFR.S YMR126C 1 1 4.4% 342 39187 7.3 U YMR126C SGDID:S000004733, Chr XIII from 521788-520760, reverse complement, Uncharacterized ORF, "Protein of unknown function, deletion causes sensitivity to thermal stress" * Jamie_FT_01_itms_14.07971.07971.3 3.2097 0.1445 98.5 1947.7444 1947.164 5970.7 182 4.158 35.7 1 K.FVKFCLY*FGEVSLTR.D Reverse_YDR055W 1 1 4.3% 444 45777 9.2 U PST1 SGDID:S000002462, Chr IV from 563524-564858, Verified ORF, "Cell wall protein that contains a putative GPI-attachment site; secreted by regenerating protoplasts; up-regulated by activation of the cell integrity pathway, as mediated by Rlm1p; upregulated by cell wall damage via disruption of FKS1" * Jamie_FT_01_itms_32.19139.19139.2 1.7287 0.0762 90.2 2135.2922 2137.3086 7593.8 206 3.184 30.6 1 K.IDGSTFKKEEAKSSS*KSVK.A YDR238C 1 1 4.2% 973 109019 5.6 U SEC26 SGDID:S000002646, Chr IV from 940810-937889, reverse complement, Verified ORF, "Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP)" * Jamie_FT_01_itms_41.24505.24505.3 3.1007 0.1871 98.5 4717.9443 4714.335 6301.6 67 4.376 16.2 1 R.NAFIGLAELDRENALHYLENNIADIENLDPLLQAVFVQFIR.Q Reverse_YBR216C 1 1 4.2% 674 77741 5.6 U YBP1 SGDID:S000000420, Chr II from 657595-655571, reverse complement, Verified ORF, "Protein required for oxidation of specific cysteine residues of the transcription factor Yap1p, resulting in the nuclear localization of Yap1p in response to stress" * Jamie_FT_03_itms_14.17329.17329.3 2.125 0.1888 92.0 3359.3943 3362.6372 8623.1 270 3.926 21.3 1 K.VLHQNT*ELYHIGSFLIVGNY*DLGVNKEK.A YOR386W 1 1 4.2% 565 66274 9.1 U PHR1 SGDID:S000005913, Chr XV from 1066835-1068532, Verified ORF, "DNA photolyase involved in photoreactivation, repairs pyrimidine dimers in the presence of visible light; induced by DNA damage; regulated by transcriptional repressor Rph1p" * Jamie_FT_03_itms_14.17282.17282.2 1.6625 0.116 93.8 2750.412 2752.106 10696.5 82 3.132 23.9 1 K.SKWCLPDVSEEAALSRLKDFLGTK.S Reverse_YFR005C 1 1 4.2% 448 52167 7.1 U SAD1 SGDID:S000001901, Chr VI from 155868-154522, reverse complement, Verified ORF, "Conserved zinc-finger domain protein involved in pre-mRNA splicing, required for assembly of U4 snRNA into the U4/U6 particle" * Jamie_FT_02_itms_17.13321.13321.2 1.5381 0.1426 93.8 2467.3123 2466.6238 10054.8 136 3.47 27.8 1 R.YKVHLIELES*S*FEVLTQNR.N Reverse_YNL041C 1 1 4.1% 839 96976 5.3 U COG6 SGDID:S000004986, Chr XIV from 551988-549469, reverse complement, Verified ORF, "Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments" * Jamie_FT_01_itms_30.17941.17941.3 3.6815 0.0831 94.9 4131.8643 4134.4175 11339.5 34 3.49 18.2 1 K.LQEAKLRYQDIEQLVVNNTPVENTENSLLKES*T*R.Q YDR194C 1 1 4.1% 664 76269 9.0 U MSS116 SGDID:S000002602, Chr IV from 847941-845947, reverse complement, Verified ORF, "DEAD-box protein required for efficient splicing of mitochondrial Group I and II introns; presumed RNA helicase due to DEAD-box motif" * Jamie_FT_01_itms_29.17135.17135.3 3.8462 0.1708 99.3 3076.7043 3075.4429 10026.4 32 3.807 23.1 1 K.VLDEADRLLEIGFRDDLETISGILNEK.N YOR073W 1 1 4.1% 590 66706 9.3 U SGO1 SGDID:S000005599, Chr XV from 464772-466544, Verified ORF, "Component of the spindle checkpoint, involved in sensing lack of tension on mitotic chromosomes; protects centromeric Rec8p at meiosis I; required for accurate chromosomal segregation at meiosis II and for mitotic chromosome stability" * Jamie_FT_01_itms_33.19227.19227.2 2.23 0.0103 90.0 2810.4922 2809.0955 7103.9 194 3.181 23.9 1 K.KVIDEVMPVSNMSSNSEISFT*RTR.R YPR083W 1 1 4.1% 579 64959 5.5 U MDM36 SGDID:S000006287, Chr XVI from 704852-706591, Verified ORF, "Protein required for normal mitochondrial morphology and inheritance" * Jamie_FT_02_itms_26.18438.18438.3 3.3259 0.125 96.2 2915.5144 2917.1345 7174.7 153 3.701 25.0 1 K.RKVEGKS*MLNDEEDLHLSKAT*LNK.C YKR048C 1 1 4.1% 417 47885 4.3 U NAP1 SGDID:S000001756, Chr XI from 526282-525029, reverse complement, Verified ORF, "Protein that interacts with mitotic cyclin Clb2p; required for the regulation of microtubule dynamics during mitosis; controls bud morphogenesis; involved in the transport of H2A and H2B histones to the nucleus" * Jamie_FT_01_itms_13.07250.07250.2 1.5145 0.1517 94.2 2135.8323 2134.1284 9708.7 312 3.649 34.4 1 K.IQNEDQDEELEEDLEER.L YGL075C 1 3 4.1% 387 44585 8.3 U MPS2 SGDID:S000003043, Chr VII from 368091-366928, reverse complement, Verified ORF, "Essential membrane protein localized at the nuclear envelope and spindle pole body (SPB), required for insertion of the newly duplicated SPB into the nuclear envelope; potentially phosphorylated by Cdc28p" * Jamie_FT_01_itms_23.13548.13548.2 2.711 0.3006 100.0 2091.9722 2092.269 11440.3 14 5.683 40.0 3 K.IVWFFEDQTDLETEYR.S Reverse_YBR065C 1 1 4.1% 364 40925 9.5 U ECM2 SGDID:S000000269, Chr II from 369676-368582, reverse complement, Verified ORF, "Pre-mRNA splicing factor, facilitates the cooperative formation of U2/U6 helix II in association with stem II in the spliceosome, function may be regulated by Slu7p" * Jamie_FT_04_itms_6.14268.14268.2 1.6058 0.2241 99.2 1854.6322 1853.898 10668.3 5 3.799 42.9 1 K.KS*TKKKDNKANDTS*K.T YJL088W 1 1 4.1% 338 37845 6.3 U ARG3 SGDID:S000003624, Chr X from 268715-269731, Verified ORF, "Ornithine carbamoyltransferase (carbamoylphosphate:L-ornithine carbamoyltransferase), catalyzes the sixth step in the biosynthesis of the arginine precursor ornithine" * Jamie_FT_01_itms_28.16566.16566.2 1.8643 0.098 94.3 1728.5322 1729.0812 6489.9 159 4.023 42.3 1 R.ILVQRAQHFKNVFK.A YAL047C 2 2 4.0% 622 72105 5.1 U SPC72 SGDID:S000000045, Chr I from 56858-54990, reverse complement, Verified ORF, "Component of the cytoplasmic Tub4p (gamma-tubulin) complex, binds spindle pole bodies and links them to microtubules; has roles in astral microtubule formation and stabilization" * Jamie_FT_01_itms_32.18893.18893.4 3.5623 0.3291 100.0 3034.2166 3032.4636 2614.3 1 5.04 33.3 1 K.TLDTQLEIVIEILHKEYDQFINSIR.L * Jamie_FT_01_itms_32.19056.19056.3 3.6357 0.2314 100.0 3036.1743 3032.4636 10917.4 27 4.268 24.0 1 K.TLDTQLEIVIEILHKEYDQFINSIR.L Reverse_YPL179W 1 1 4.0% 549 61421 9.3 U PPQ1 SGDID:S000006100, Chr XVI from 208156-209805, Verified ORF, "Putative protein serine/threonine phosphatase; null mutation enhances efficiency of translational suppressors" * Jamie_FT_02_itms_32.22373.22373.2 1.9159 0.1219 96.8 2260.912 2263.3396 10580.8 29 3.6 28.6 1 K.KSSKPNPIGSTPS*SPAGFS*GAK.V Reverse_YJR122W 1 1 4.0% 497 57055 7.9 U CAF17 SGDID:S000003883, Chr X from 649691-651184, Verified ORF, "Mitochondrial protein that interacts with Ccr4p in the two-hybrid system; 3'-untranslated region contains a putative mRNA localization element common to genes encoding mitochondrial proteins" * Jamie_FT_01_itms_28.16404.16404.2 2.3565 0.1311 99.3 2191.112 2193.5083 9612.1 359 3.485 28.9 1 R.LLAVGYLGENS*ILLGAPRQK.R YGR264C 2 3 3.9% 751 85678 6.6 U MES1 SGDID:S000003496, Chr VII from 1021859-1019604, reverse complement, Verified ORF, "Methionyl-tRNA synthetase, forms a complex with glutamyl-tRNA synthetase (Gus1p) and Arc1p, which increases the catalytic efficiency of both tRNA synthetases; also has a role in nuclear export of tRNAs" * Jamie_FT_02_itms_30.21247.21247.3 4.0894 0.2683 100.0 3051.8342 3051.5654 9875.6 1 5.248 28.6 2 K.SDAVVAVGLNIIYAVSSIITPYMPEIGEK.I * Jamie_FT_02_itms_30.21236.21236.2 2.0421 0.1062 97.1 3052.3523 3051.5654 9482.8 37 4.1 23.2 1 K.SDAVVAVGLNIIYAVSSIITPYMPEIGEK.I YDR502C 1 1 3.9% 384 42256 5.4 U SAM2 SGDID:S000002910, Chr IV from 1454454-1453300, reverse complement, Verified ORF, "S-adenosylmethionine synthetase, catalyzes transfer of the adenosyl group of ATP to the sulfur atom of methionine; one of two differentially regulated isozymes (Sam1p and Sam2p)" YLR180W 1 1 3.9% 382 41818 5.2 U SAM1 SGDID:S000004170, Chr XII from 515264-516412, Verified ORF, "S-adenosylmethionine synthetase, catalyzes transfer of the adenosyl group of ATP to the sulfur atom of methionine; one of two differentially regulated isozymes (Sam1p and Sam2p)" Jamie_FT_01_itms_12.06710.06710.2 2.3749 0.2166 100.0 1445.2122 1445.6182 7682.6 1 4.667 64.3 1 R.FVIGGPQGDAGLTGR.K YMR055C 1 1 3.9% 306 35028 8.6 U BUB2 SGDID:S000004659, Chr XIII from 387020-386100, reverse complement, Verified ORF, "Mitotic exit network regulator, forms GTPase-activating Bfa1p-Bub2p complex that binds Tem1p and spindle pole bodies, blocks cell cycle progression before anaphase in response to spindle and kinetochore damage" * Jamie_FT_01_itms_18.10393.10393.2 2.1072 0.1154 97.9 1466.5721 1466.5902 7958.2 100 3.41 45.5 1 R.QFPDFDADEIIR.L Reverse_YJR127C 1 1 3.8% 1380 155062 8.0 U RSF2 SGDID:S000003888, Chr X from 662974-658832, reverse complement, Verified ORF, "Zinc-finger protein involved in transcriptional control of both nuclear and mitochondrial genes, many of which specify products required for glycerol-based growth, respiration, and other functions" * Jamie_FT_04_itms_15.19234.19234.4 2.5372 0.1646 94.3 6175.7363 6181.102 8158.0 94 3.217 13.1 1 K.AFLYVPIILRMAPNANIT*S*MSNETPTLIGGNKLYLNEWYKLMTDIRQRSTMK.W YMR186W 1 5 3.8% 705 80900 4.8 U HSC82 SGDID:S000004798, Chr XIII from 632354-634471, Verified ORF, "Cytoplasmic chaperone of the Hsp90 family, redundant in function and nearly identical with Hsp82p, and together they are essential; expressed constitutively at 10-fold higher basal levels that HSP82 and induced 2-3 fold by heat shock" * Jamie_FT_01_itms_35.20533.20533.2 4.383 0.4352 100.0 2963.412 2961.2952 11823.4 1 7.31 40.4 5 K.DLTNLLFETALLTSGFSLEEPTSFASR.I YHR020W 1 1 3.8% 688 77386 6.4 U YHR020W SGDID:S000001062, Chr VIII from 143989-146055, Uncharacterized ORF, "Protein required for cell viability" * Jamie_FT_01_itms_40.23546.23546.2 2.6356 0.2851 95.5 2940.4521 2938.3875 8974.4 1 5.423 28.0 1 D.AEEEVLQILDFYAGVYEELLAVPVVK.G Reverse_YDL070W 1 1 3.8% 638 72513 6.1 U BDF2 SGDID:S000002228, Chr IV from 331025-332941, Verified ORF, "Protein involved in transcription initiation at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf1p" * Jamie_FT_02_itms_9.08420.08420.2 1.15 0.2848 97.8 2524.372 2522.758 4734.0 57 4.109 15.2 1 R.SNATASQPAALLASHAHRTDMNTR.S YOR204W 2 3 3.8% 604 65553 7.8 U DED1 SGDID:S000005730, Chr XV from 722911-724725, Verified ORF, "ATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase, required for translation initiation of all yeast mRNAs; mutations in human DEAD-box DBY are a frequent cause of male infertility" * Jamie_FT_01_itms_35.20750.20750.2 3.143 0.2584 100.0 2310.6921 2308.6746 7418.6 11 4.657 35.7 2 K.SALLDLLSASTDGLTLIFVETK.R * Jamie_FT_01_itms_32.18804.18804.2 2.4407 0.1432 99.5 2467.5923 2464.862 8601.7 56 3.797 25.0 1 K.SALLDLLSASTDGLTLIFVETKR.M Reverse_YPL086C 1 1 3.8% 557 63657 9.0 U ELP3 SGDID:S000006007, Chr XVI from 386443-384770, reverse complement, Verified ORF, "Histone acetyltransferase subunit of the Elongator complex, which is a component of the RNA polymerase II holoenzyme; activity is directed specifically towards histones H3 and H4; disruption confers resistance to K. lactis zymotoxin" * Jamie_FT_01_itms_32.18936.18936.2 2.2944 0.0616 95.8 2221.4521 2221.6787 9580.7 1 3.485 35.0 1 R.HPKCMVAVVAIGSATRVPKAK.L YIR019C 1 1 3.7% 1367 136110 4.3 U MUC1 SGDID:S000001458, Chr IX from 393672-389569, reverse complement, Verified ORF, "GPI-anchored cell surface glycoprotein required for diploid pseudohyphal formation and haploid invasive growth, transcriptionally regulated by the MAPK pathway (via Ste12p and Tec1p) and the cAMP pathway (via Flo8p)" * Jamie_FT_01_itms_26.15410.15410.4 3.847 0.0686 94.7 4996.8965 4995.1953 8299.5 210 2.832 14.6 1 K.TIVSSASAGENTAPSATTPVTTAIPTT*VITTESSVGTNS*AGETTTGYTTK.S Reverse_YPL226W 1 1 3.7% 1196 134330 5.9 U NEW1 SGDID:S000006147, Chr XVI from 121767-125357, Verified ORF, "ATP binding cassette family member; Asn/Gln-rich rich region supports [NU+] prion formation, susceptibility to [PSI+] prion induction and aggregation of a fragment of the human Machado-Joseph Disease protein" * Jamie_FT_01_itms_30.17652.17652.4 3.521 0.0824 93.9 4777.3364 4772.1875 10244.3 1 2.837 16.3 1 K.ILTSKGAGNPGLCAVRS*SLSLSCSVHSLSPKQAGPYSFTVDT*MK.A YER033C 1 1 3.7% 1076 119350 4.9 U ZRG8 SGDID:S000000835, Chr V from 221286-218056, reverse complement, Verified ORF, "Cytoplasmic protein of unknown function, transcription is induced under conditions of zinc deficiency" * Jamie_FT_04_itms_11.16891.16891.4 3.1032 0.1102 93.9 4507.8965 4502.5674 9381.8 280 3.5 16.7 1 R.HSNFSGNTNNSS*QRSSDDSLDFLPSTPSQMNY*DSIPPPAK.H YGL234W 1 3 3.7% 802 86068 5.3 U ADE5,7 SGDID:S000003203, Chr VII from 56482-58890, Verified ORF, "Bifunctional enzyme of the 'de novo' purine nucleotide biosynthetic pathway, contains aminoimidazole ribotide synthetase and glycinamide ribotide synthetase activities" * Jamie_FT_01_itms_33.19697.19697.3 6.5088 0.5904 100.0 3275.2144 3272.5957 8526.6 1 9.972 33.6 3 R.FGDPETQAVLSLLDDQTDLAQVFLAAAEHR.L Reverse_YLR429W 1 1 3.7% 651 72553 5.9 U CRN1 SGDID:S000004421, Chr XII from 990773-992728, Verified ORF, "Coronin, cortical actin cytoskeletal component that associates with the Arp2p/Arp3p complex to regulate its activity" * Jamie_FT_02_itms_12.10340.10340.3 2.9425 0.2563 99.6 2738.1543 2740.091 10558.8 122 5.025 23.9 1 K.EINFADWIGIQRDSLKSFGTTALR.D Reverse_YIL002C 1 1 3.6% 946 108430 6.7 U INP51 SGDID:S000001264, Chr IX from 353428-350588, reverse complement, Verified ORF, "Phosphatidylinositol 4,5-bisphosphate 5-phosphatase, synaptojanin-like protein with an N-terminal Sac1 domain, plays a role in phosphatidylinositol 4,5-bisphosphate homeostasis and in endocytosis; null mutation confers cold-tolerant growth" * Jamie_FT_04_itms_6.14113.14113.4 2.515 0.1521 92.7 4127.4966 4125.4434 8464.2 6 3.811 19.2 1 K.TGPDFKYT*PPFDIAMEHFYPFS*EGAIMQQNLQDK.E YDR362C 1 1 3.6% 672 74709 6.6 U TFC6 SGDID:S000002770, Chr IV from 1198687-1196669, reverse complement, Verified ORF, "One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; cooperates with Tfc3p in DNA binding; human homolog is TFIIIC-110" * Jamie_FT_01_itms_32.19033.19033.2 2.0315 0.0977 96.3 2629.8323 2626.9077 8594.5 18 4.077 26.1 1 K.KNSTKNMKS*SSPGSSLGQKGRPIR.L YBR189W 1 1 3.6% 195 22299 10.1 U RPS9B SGDID:S000000393, Chr II from 604503-604509,604923-605503, Verified ORF, "Protein component of the small (40S) ribosomal subunit; nearly identical to Rps9Bp and has similarity to E. coli S4 and rat S9 ribosomal proteins" YPL081W 1 1 3.6% 197 22443 10.0 U RPS9A SGDID:S000006002, Chr XVI from 404947-404953,405455-406041, Verified ORF, "Protein component of the small (40S) ribosomal subunit; nearly identical to Rps9Ap and has similarity to E. coli S4 and rat S9 ribosomal proteins" Jamie_FT_01_itms_10.05729.05729.2 1.9596 0.0119 92.6 909.6722 907.99884 4832.2 8 3.995 91.7 1 K.VEDFLER.R Reverse_YOR188W 1 1 3.5% 1137 129971 8.9 U MSB1 SGDID:S000005714, Chr XV from 685767-689180, Verified ORF, "Protein involved in positive regulation of both 1,3-beta-glucan synthesis and the Pkc1p-MAPK pathway, potential Cdc28p substrate; multicopy suppressor of temperature-sensitive mutations in CDC24 and CDC42, and of mutations in BEM4" * Jamie_FT_01_itms_25.14849.14849.4 3.1963 0.0946 92.9 4458.0166 4459.442 9370.6 78 2.696 15.8 1 R.DATYKSSDACSDNTNTSLKLSHVAS*SHSSQLDDDT*INSPK.Y YKL038W 1 1 3.5% 1170 128240 7.9 U RGT1 SGDID:S000001521, Chr XI from 365248-368760, Verified ORF, "Glucose-responsive transcription factor that regulates expression of several glucose transporter (HXT) genes in response to glucose; binds to promoters and acts both as a transcriptional activator and repressor" * Jamie_FT_02_itms_18.13950.13950.4 3.5586 0.1408 97.1 4148.4966 4143.401 9196.8 284 3.476 16.2 1 R.PPNPPANNPT*VQEGPSAMGSSPVAGNLSAAPPSEGNPDFYK.K Reverse_YER033C 1 1 3.5% 1076 119350 4.9 U ZRG8 SGDID:S000000835, Chr V from 221286-218056, reverse complement, Verified ORF, "Cytoplasmic protein of unknown function, transcription is induced under conditions of zinc deficiency" * Jamie_FT_02_itms_21.15421.15421.4 3.3554 0.0727 90.0 4164.5767 4165.4307 9281.3 77 2.528 17.6 1 K.VTPKTSGTTGT*LSAKIASTHETEDDSNNRKLSFSYNPK.G YER091C 2 3 3.5% 767 85860 6.5 U MET6 SGDID:S000000893, Chr V from 342163-339860, reverse complement, Verified ORF, "Cobalamin-independent methionine synthase, involved in amino acid biosynthesis; requires a minimum of two glutamates on the methyltetrahydrofolate substrate, similar to bacterial metE homologs" * Jamie_FT_01_itms_38.22631.22631.3 4.0404 0.1658 99.3 3058.4043 3058.5366 5877.5 3 4.901 29.8 1 K.ADKDSLDLEPLSLLEQLLPLYTEILSK.L * Jamie_FT_01_itms_38.22639.22639.2 3.7352 0.2929 100.0 3059.8323 3058.5366 6550.0 1 5.593 36.5 2 K.ADKDSLDLEPLSLLEQLLPLYTEILSK.L YKR024C 1 1 3.5% 742 83308 9.3 U DBP7 SGDID:S000001732, Chr XI from 487015-484787, reverse complement, Verified ORF, "Putative ATP-dependent RNA helicase of the DEAD-box family involved in ribosomal biogenesis" * Jamie_FT_01_itms_36.21501.21501.2 2.0785 0.029 90.3 3128.672 3130.3584 9724.2 20 3.131 24.0 1 K.DVNVNRNDKFIRKDEKS*SKNKDVGDK.E Reverse_YHL047C 1 1 3.5% 637 71554 8.4 U ARN2 SGDID:S000001039, Chr VIII from 10211-8298, reverse complement, Verified ORF, "Transporter, member of the ARN family of transporters that specifically recognize siderophore-iron chelates; responsible for uptake of iron bound to the siderophore triacetylfusarinine C" * Jamie_FT_03_itms_14.17297.17297.2 1.994 0.1489 98.2 2752.0322 2754.9702 9932.0 4 3.933 26.2 1 R.KAIWDNIPDDEKT*QVYERDPLK.Q YDR023W 1 1 3.5% 462 53310 6.1 U SES1 SGDID:S000002430, Chr IV from 489504-490892, Verified ORF, "Cytosolic seryl-tRNA synthetase, class II aminoacyl-tRNA synthetase that aminoacylates tRNA(Ser), displays tRNA-dependent amino acid recognition which enhances discrimination of the serine substrate, interacts with peroxin Pex21p" * Jamie_FT_04_itms_9.16117.16117.2 1.4132 0.1611 93.8 1745.3522 1743.7789 6702.3 347 3.899 30.0 1 K.PEDLEAVGPIAS*VT*GK.P YJL158C 1 1 3.5% 227 23242 4.7 U CIS3 SGDID:S000003694, Chr X from 122865-122182, reverse complement, Verified ORF, "Mannose-containing glycoprotein constituent of the cell wall; member of the PIR (proteins with internal repeats) family" YKL164C 1 1 2.3% 341 34622 6.5 U PIR1 SGDID:S000001647, Chr XI from 142824-141799, reverse complement, Verified ORF, "O-glycosylated protein required for cell wall stability; attached to the cell wall via beta-1,3-glucan; mediates mitochondrial translocation of Apn1p; expression regulated by the cell integrity pathway and by Swi5p during the cell cycle" YKL163W 1 1 2.5% 325 33004 5.7 U PIR3 SGDID:S000001646, Chr XI from 144406-145383, Verified ORF, "O-glycosylated covalently-bound cell wall protein required for cell wall stability; expression is cell cycle regulated, peaking in M/G1 and also subject to regulation by the cell integrity pathway" YJL160C 1 1 2.8% 287 30215 9.0 U YJL160C SGDID:S000003696, Chr X from 118820-117957, reverse complement, Uncharacterized ORF, "Hypothetical protein" YJL159W 1 1 2.1% 387 38619 5.4 U HSP150 SGDID:S000003695, Chr X from 120444-121607, Verified ORF, "O-mannosylated heat shock protein that is secreted and covalently attached to the cell wall via beta-1,3-glucan and disulfide bridges; required for cell wall stability; induced by heat shock, oxidative stress, and nitrogen limitation" Jamie_FT_01_itms_8.04463.04463.2 2.0736 0.2237 100.0 830.6322 829.9749 4352.5 52 5.452 71.4 1 R.IGSIVANR.Q Reverse_YDR478W 1 1 3.5% 198 22541 9.6 U SNM1 SGDID:S000002886, Chr IV from 1414565-1415161, Verified ORF, "Subunit of RNase MRP, which cleaves pre-rRNA; not shared between Rnase MRP and nuclear Rnase P, in contrast to all other Rnase MRP protein subunits; binds to the NME1 RNA subunit of Rnase MRP" * Jamie_FT_01_itms_12.07003.07003.2 2.1214 0.0247 95.7 875.1922 874.9695 4327.6 56 2.803 91.7 1 K.KVAY*SVK.G YGR061C 2 2 3.4% 1358 148904 5.3 U ADE6 SGDID:S000003293, Chr VII from 615969-611893, reverse complement, Verified ORF, "Formylglycinamidine-ribonucleotide (FGAM)-synthetase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway" * Jamie_FT_01_itms_36.21261.21261.2 2.8899 0.2764 100.0 1795.4521 1795.104 10260.8 1 5.802 46.9 1 R.SDGGLLITLLEMAFASR.C * Jamie_FT_01_itms_27.16158.16158.4 3.2171 0.1082 94.6 3568.8564 3572.8071 6787.3 129 2.842 21.4 1 R.SQFS*KFFNERQDTFAFGACNGCQFLSRLK.D YOR206W 1 1 3.4% 710 81601 8.5 U NOC2 SGDID:S000005732, Chr XV from 727512-729644, Verified ORF, "Protein that forms a nucleolar complex with Mak21p that binds to 90S and 66S pre-ribosomes, as well as a nuclear complex with Noc3p that binds to 66S pre-ribosomes; both complexes mediate intranuclear transport of ribosomal precursors" * Jamie_FT_02_itms_21.15657.15657.2 2.1566 0.3529 100.0 2803.112 2804.3958 9338.6 1 6.113 32.6 1 P.VIISLRRYIKTSTNVKLNKRLSTV.V Reverse_YDR295C 1 1 3.4% 674 77059 8.7 U HDA2 SGDID:S000002703, Chr IV from 1054641-1052617, reverse complement, Verified ORF, "Subunit of a possibly tetrameric trichostatin A-sensitive class II histone deacetylase complex that contains an Hda1p homodimer and an Hda2p-Hda3p heterodimer; required for the activity of the complex; has similarity to Hda3p; Ploidy-related" * Jamie_FT_04_itms_19.21604.21604.2 1.3334 0.204 96.0 2745.0723 2745.9717 3971.2 53 3.87 18.2 1 R.FRTGNAAAKEVQVNNKNKLDY*QR.N YLR088W 1 1 3.4% 614 69221 6.3 U GAA1 SGDID:S000004078, Chr XII from 316108-317952, Verified ORF, "Subunit of the GPI (glycosylphosphatidylinositol):protein transamidase complex, removes the GPI-anchoring signal and attaches GPI to proteins in the ER" * Jamie_FT_01_itms_19.10935.10935.4 3.415 0.0625 92.0 2482.0166 2483.9216 8441.6 2 3.332 33.3 1 R.WPVWSKNIIVVFSENPRAALR.S YLR029C 1 1 3.4% 204 24422 11.4 U RPL15A SGDID:S000004019, Chr XII from 202591-201977, reverse complement, Verified ORF, "Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl15Bp and has similarity to rat L15 ribosomal protein; binds to 5.8 S rRNA" YMR121C 1 1 3.4% 204 24422 11.4 U RPL15B SGDID:S000004728, Chr XIII from 510347-509733, reverse complement, Verified ORF, "Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl15Ap and has similarity to rat L15 ribosomal protein; binds to 5.8 S rRNA" Jamie_FT_01_itms_11.06271.06271.2 1.6576 0.1931 99.5 883.03217 883.03784 6348.9 139 3.74 66.7 1 K.QGFVIYR.V Reverse_YLL034C 1 1 3.3% 837 93069 5.4 U RIX7 SGDID:S000003957, Chr XII from 73145-70632, reverse complement, Verified ORF, "Putative ATPase of the AAA family, required for export of pre-ribosomal large subunits from the nucleus; distributed between the nucleolus, nucleoplasm, and nuclear periphery depending on growth conditions" * Jamie_FT_04_itms_4.12232.12232.3 2.7095 0.2327 98.4 3331.5842 3335.775 10332.0 58 4.205 22.2 1 K.EET*NPLEIFLSKDLRGPRLMAPDIMDPR.N YOL152W 1 1 3.3% 629 71997 9.1 U FRE7 SGDID:S000005512, Chr XV from 40747-42636, Verified ORF, "Putative ferric reductase with similarity to Fre2p; expression induced by low copper levels" * Jamie_FT_01_itms_38.22404.22404.2 2.8992 0.1564 100.0 2308.632 2307.8276 10169.4 4 4.551 37.5 1 R.SGVPPILFLNLLWLSSLPIAR.R YBR139W 1 1 3.3% 508 57639 5.3 U YBR139W SGDID:S000000343, Chr II from 515658-517184, Uncharacterized ORF, "Putative serine type Carboxypeptidase; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; YBR139W is not an essential gene" * Jamie_FT_01_itms_37.21768.21768.2 1.719 0.1415 96.3 2129.632 2129.4656 9617.0 17 3.698 37.5 1 K.DAYIFLELFFEAFPHLR.S YCR053W 1 1 3.3% 514 57474 5.6 U THR4 SGDID:S000000649, Chr III from 216692-218236, Verified ORF, "Threonine synthase, conserved protein that catalyzes formation of threonine from 0-phosphohomoserine; expression is regulated by the GCN4-mediated general amino acid control pathway" * Jamie_FT_01_itms_37.22089.22089.2 3.3608 0.383 100.0 2060.7922 2060.3599 7410.3 1 6.812 50.0 1 K.DVALQFVGNLFEYFLQR.T Reverse_YNR069C 1 1 3.3% 489 54831 5.8 U BSC5 SGDID:S000005352, Chr XIV from 762592-761123, reverse complement, Verified ORF, "Protein of unknown function, ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression" * Jamie_FT_02_itms_11.09425.09425.2 1.7088 0.1563 97.3 1849.4922 1847.8926 6101.0 189 3.917 33.3 1 K.LT*GNETNGAVANWEYK.G YBR218C 2 5 3.2% 1180 130167 6.5 U PYC2 SGDID:S000000422, Chr II from 662244-658702, reverse complement, Verified ORF, "Pyruvate carboxylase isoform, cytoplasmic enzyme that converts pyruvate to oxaloacetate; highly similar to isoform Pyc1p but differentially regulated; mutations in the human homolog are associated with lactic acidosis" * Jamie_FT_02_itms_23.16956.16956.4 3.728 0.1384 98.2 4031.4565 4033.6091 12893.1 4 3.688 19.4 1 R.IQVEHTITEEITGIDIVSAQIQIAAGATLTQLGLLQDK.I * Jamie_FT_03_itms_16.18538.18538.3 6.7574 0.4012 100.0 4036.2844 4033.6091 9131.5 1 7.707 25.7 4 R.IQVEHTITEEITGIDIVSAQIQIAAGATLTQLGLLQDK.I YBR059C 1 1 3.2% 1108 123989 7.7 U AKL1 SGDID:S000000263, Chr II from 360185-356859, reverse complement, Verified ORF, "Ser-Thr protein kinase, member (with Ark1p and Prk1p) of the Ark kinase family; involved in endocytosis and actin cytoskeleton organization" * Jamie_FT_01_itms_40.23528.23528.4 2.7 0.1858 96.7 4068.8564 4074.3076 9789.1 70 3.673 19.0 1 K.LSDSEENLLNDMFLSSFEISS*KLPMNAS*DGHAAVSR.I Reverse_YDR186C 2 2 3.2% 877 98331 5.4 U YDR186C SGDID:S000002594, Chr IV from 835487-832854, reverse complement, Uncharacterized ORF, "Hypothetical protein" * Jamie_FT_01_itms_12.07107.07107.2 2.1466 0.0449 96.7 878.65216 877.0311 4712.3 3 2.656 85.7 1 K.FNLALGNK.E * Jamie_FT_01_itms_29.17208.17208.2 2.1559 0.0868 96.2 2226.132 2227.309 11085.2 167 3.229 28.9 1 K.DNDEATSTNSFSQAISNRLR.H YGL125W 1 1 3.2% 600 68560 5.8 U MET13 SGDID:S000003093, Chr VII from 272526-274328, Verified ORF, "Isozyme of methylenetetrahydrofolate reductase, catalyzes the reduction of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate in the methionine biosynthesis pathway" * Jamie_FT_01_itms_34.19911.19911.2 2.5788 0.1147 99.5 2597.412 2595.8457 9922.5 235 3.9 30.6 1 K.EEFY*HILNEWKLNMNKYDK.P Reverse_YOR377W 1 1 3.2% 525 61036 7.0 U ATF1 SGDID:S000005904, Chr XV from 1046222-1047799, Verified ORF, "Alcohol acetyltransferase with potential roles in lipid and sterol metabolism; responsible for the major part of volatile acetate ester production during fermentation" * Jamie_FT_05_itms_8.18033.18033.2 1.3774 0.2631 99.0 1954.2522 1956.123 6252.5 43 4.592 25.0 1 K.YISCLEELSEQSGVVNK.T Reverse_YAR002W 1 1 3.2% 539 59039 9.3 U NUP60 SGDID:S000000063, Chr I from 152259-153878, Verified ORF, "Subunit of the nuclear pore complex (NPC), functions to anchor Nup2p to the NPC in a dynamic process that is controlled by the nucleoplasmic concentration of Gsp1p-GTP; potential Cdc28p substrate" * Jamie_FT_01_itms_26.15560.15560.2 1.7774 0.0974 93.6 2078.0522 2077.2246 9245.0 24 3.187 34.4 1 K.RY*PASPVTASARRLS*KR.H YHR070W 1 1 3.2% 499 56514 8.8 U TRM5 SGDID:S000001112, Chr VIII from 234883-236382, Verified ORF, "tRNA(m(1)G37)methyltransferase, methylates a tRNA base adjacent to the anticodon that has a role in prevention of frameshifting; highly conserved across Archaea, Bacteria, and Eukarya" * Jamie_FT_04_itms_7.14907.14907.2 1.7167 0.1335 95.7 1898.9521 1899.0648 8479.8 237 4.268 36.7 1 K.KDVIVLANDLNPES*YK.Y YHL012W 1 1 3.2% 493 56000 5.3 U YHL012W SGDID:S000001004, Chr VIII from 78932-80413, Uncharacterized ORF, "Hypothetical protein" * Jamie_FT_01_itms_23.13517.13517.2 1.8261 0.0748 92.1 1731.0322 1731.0891 9552.9 181 3.651 36.7 1 K.LAILKLT*GKANPIIGK.E Reverse_YBR079C 1 1 3.1% 964 110344 6.3 U RPG1 SGDID:S000000283, Chr II from 398271-395377, reverse complement, Verified ORF, "Subunit of the core complex of translation initiation factor 3(eIF3), essential for translation; part of a subcomplex (Prt1p-Rpg1p-Nip1p) that stimulates binding of mRNA and tRNA(i)Met to ribosomes" * Jamie_FT_01_itms_25.14760.14760.3 3.2794 0.2163 99.3 3587.3643 3587.981 5986.5 16 3.685 23.3 1 K.RTAEALEQARKQREANAIEEKRRAIAEEYR.R Reverse_YFL054C 1 1 3.1% 646 70507 8.4 U YFL054C SGDID:S000001840, Chr VI from 22787-20847, reverse complement, Uncharacterized ORF, "Hypothetical protein" * Jamie_FT_01_itms_39.23019.23019.2 1.9545 0.0832 94.1 2228.9722 2227.278 6405.4 114 3.837 31.6 1 K.LTPPRTSESSSSSRGS*EYSM.- YIL121W 1 1 3.1% 542 59618 9.1 U QDR2 SGDID:S000001383, Chr IX from 132241-133869, Verified ORF, "Multidrug transporter required for resistance to quinidine, barban, cisplatin, and bleomycin; member of the major facilitator superfamily of transporters conferring multiple drug resistance (MFS-MDR)" * Jamie_FT_02_itms_22.16323.16323.2 1.8265 0.0779 92.7 2043.4521 2041.2904 11595.2 12 2.953 34.4 1 R.CILS*AVFIAALS*KMVEK.M YDR130C 1 1 3.1% 291 33186 9.8 U FIN1 SGDID:S000002537, Chr IV from 716617-715742, reverse complement, Verified ORF, "Spindle pole body-related intermediate filament protein, forms cell cycle-specific filaments between spindle pole bodies in mother and daughter cells, able to self-assemble, expression induced during S/G2, localization cell-cycle dependent" * Jamie_FT_01_itms_11.06360.06360.2 1.8952 0.0863 96.3 956.2322 955.9572 4095.0 2 3.122 81.2 1 K.IGGDGSLT*R.I YLR441C 1 2 3.1% 255 28743 10.0 U RPS1A SGDID:S000004433, Chr XII from 1018904-1018137, reverse complement, Verified ORF, "Ribosomal protein 10 (rp10) of the small (40S) subunit; nearly identical to Rps1Bp and has similarity to rat S3a ribosomal protein" YML063W 1 2 3.1% 255 28812 10.0 U RPS1B SGDID:S000004528, Chr XIII from 146482-147249, Verified ORF, "Ribosomal protein 10 (rp10) of the small (40S) subunit; nearly identical to Rps1Ap and has similarity to rat S3a ribosomal protein" Jamie_FT_01_itms_15.08725.08725.2 1.8617 0.1948 99.6 939.47217 939.1454 4667.8 1 4.42 85.7 2 R.IFAIAFTR.K Reverse_YGL197W 1 1 3.0% 1487 167074 7.7 U MDS3 SGDID:S000003165, Chr VII from 124703-129166, Verified ORF, "Protein with an N-terminal kelch-like domain, putative negative regulator of early meiotic gene expression; required, with Pmd1p, for growth under alkaline conditions" * Jamie_FT_01_itms_28.16463.16463.4 3.8078 0.0651 94.1 4594.2964 4597.7983 8095.6 31 3.111 15.2 1 R.SGQINMS*NPFGISGVSNHSDRPGGAFVT*PVNTSSTELSGAGTATK.E YJR030C 1 1 3.0% 745 84290 7.2 U YJR030C SGDID:S000003791, Chr X from 486110-483873, reverse complement, Uncharacterized ORF, "Hypothetical protein" * Jamie_FT_01_itms_18.10727.10727.4 2.9939 0.1341 95.9 2616.1365 2616.6743 8416.3 9 3.253 27.8 1 R.S*GSKISQQVY*VRSNNPDYTSPK.R YHL019C 1 1 3.0% 605 69990 6.9 U APM2 SGDID:S000001011, Chr VIII from 69545-67728, reverse complement, Verified ORF, "Protein of unknown function, homologous to the medium chain of mammalian clathrin-associated protein complex; involved in vesicular transport" * Jamie_FT_04_itms_14.18822.18822.2 1.6943 0.2895 100.0 2082.6921 2082.5012 9817.2 15 4.785 35.3 1 K.KKRKKKKGT*KGKSVGKSK.L Reverse_YER156C 1 1 3.0% 338 38174 7.5 U YER156C SGDID:S000000958, Chr V from 484336-483320, reverse complement, Uncharacterized ORF, "Hypothetical protein" * Jamie_FT_01_itms_17.09722.09722.2 1.83 0.0451 90.6 1109.7122 1108.3292 4928.1 1 4.233 66.7 1 R.LGRLPEPLGR.R YKL210W 1 1 2.9% 1024 114266 5.1 U UBA1 SGDID:S000001693, Chr XI from 39164-42238, Verified ORF, "Ubiquitin activating enzyme, involved in ubiquitin-mediated protein degradation and essential for viability" * Jamie_FT_01_itms_37.21954.21954.2 2.6605 0.1029 99.5 3432.0923 3431.6501 11618.9 2 3.682 24.1 1 K.VGPETEEIFNDSFWESLDFVTNALDNVDAR.T YDR475C 1 1 2.9% 876 98691 9.6 U YDR475C SGDID:S000002883, Chr IV from 1410084-1407454, reverse complement, Uncharacterized ORF, "Protein of unknown function; previously annotated as two separate ORFs, YDR474C and YDR475C, which were merged as a result of corrections to the systematic reference sequence" * Jamie_FT_04_itms_33.31608.31608.4 3.1559 0.1115 94.4 2722.6165 2728.0098 11429.5 58 3.669 25.0 1 R.SMLDVLDNPSSSRSKSKSRSSSKSR.I YHR047C 1 1 2.9% 856 97663 5.3 U AAP1' SGDID:S000001089, Chr VIII from 201303-198733, reverse complement, Verified ORF, "Arginine/alanine aminopeptidase, overproduction stimulates glycogen accumulation" * Jamie_FT_01_itms_39.23191.23191.2 2.0736 0.0361 91.1 2921.632 2922.3477 10506.0 6 2.951 27.1 1 K.STWVFEPEDILNALDKFTLDLVLNK.L Reverse_YMR075W 1 1 2.9% 684 78837 8.8 U YMR075W SGDID:S000004680, Chr XIII from 413981-416035, Uncharacterized ORF, "Nuclear protein of unknown function" * Jamie_FT_01_itms_26.15236.15236.2 2.35 0.2992 92.9 2361.5322 2358.6282 10282.3 256 4.591 28.9 1 N.WGITSRINSEETMNETLFTK.L YBL060W 1 1 2.9% 687 78768 6.4 U YBL060W SGDID:S000000156, Chr II from 107934-109997, Uncharacterized ORF, "Hypothetical protein" * Jamie_FT_01_itms_40.23817.23817.2 1.6664 0.0849 90.1 2416.872 2417.6836 8087.4 3 3.064 34.2 1 K.SFFGWLKPSKTTTLIEHT*SR.R YOR137C 1 1 2.9% 622 71846 7.7 U SIA1 SGDID:S000005663, Chr XV from 583681-581813, reverse complement, Verified ORF, "Protein of unassigned function involved in activation of the Pma1p plasma membrane H+-ATPase by glucose" * Jamie_FT_04_itms_27.26656.26656.2 2.0414 0.448 100.0 2145.0723 2146.1882 5197.1 1 5.806 35.3 1 D.T*DNIAVFGEEWVY*KGSGI.W YKL081W 1 2 2.9% 412 46520 7.8 U TEF4 SGDID:S000001564, Chr XI from 282535-282739,283066-284099, Verified ORF, "Translation elongation factor EF-1 gamma" * Jamie_FT_01_itms_18.10667.10667.2 2.4502 0.0891 98.8 1408.6122 1406.578 9118.2 17 4.177 59.1 2 R.NYASEALISYFK.L YPL160W 2 2 2.8% 1090 124141 5.8 U CDC60 SGDID:S000006081, Chr XVI from 246989-250261, Verified ORF, "Cytosolic leucyl tRNA synthetase, ligates leucine to the appropriate tRNA" * Jamie_FT_01_itms_37.22074.22074.3 3.5119 0.2259 100.0 3610.2244 3609.1125 5775.6 1 4.445 23.3 1 R.LPWDEKYLVESLSDSTIYQSFYTIAHLLFK.D * Jamie_FT_01_itms_35.20870.20870.3 4.6702 0.3233 100.0 2840.2444 2840.245 5939.8 1 5.592 31.5 1 K.YLVESLSDSTIYQSFYTIAHLLFK.D YAL035W 1 2 2.8% 1002 112268 5.8 U FUN12 SGDID:S000000033, Chr I from 76428-79436, Verified ORF, "GTPase, required for general translation initiation by promoting Met-tRNAiMet binding to ribosomes and ribosomal subunit joining; homolog of bacterial IF2" * Jamie_FT_01_itms_38.22501.22501.2 3.9154 0.3618 100.0 3045.5923 3043.5708 4636.6 1 5.617 38.9 2 K.YVSIVPTSAVTGEGVPDLLWLLLELTQK.R YAR019C 1 1 2.8% 974 110285 8.0 U CDC15 SGDID:S000000072, Chr I from 175133-172209, reverse complement, Verified ORF, "Protein kinase of the Mitotic Exit Network that is localized to the spindle pole bodies at late anaphase; promotes mitotic exit by directly switching on the kinase activity of Dbf2p" * Jamie_FT_01_itms_23.13399.13399.4 2.8295 0.129 93.8 3244.1765 3246.6445 7459.5 64 3.752 23.1 1 K.LIFSKSLLKLYDYTGQPDPIKQT*EPNR.R YGR162W 2 2 2.8% 952 107101 6.0 U TIF4631 SGDID:S000003394, Chr VII from 824064-826922, Verified ORF, "Translation initiation factor eIF4G, subunit of the mRNA cap-binding protein complex (eIF4F) that also contains eIF4E (Cdc33p); associates with the poly(A)-binding protein Pab1p, also interacts with eIF4A (Tif1p); homologous to Tif4632p" * Jamie_FT_01_itms_43.25339.25339.3 4.9964 0.3995 100.0 2847.2644 2848.2231 8045.8 1 7.646 33.7 1 R.TGQATLEGSQLLDSLFGILDNIIQTAK.I * Jamie_FT_01_itms_43.25439.25439.2 2.97 0.3327 100.0 2847.892 2848.2231 10573.4 1 6.605 32.7 1 R.TGQATLEGSQLLDSLFGILDNIIQTAK.I Reverse_YBR179C 1 1 2.8% 855 97808 7.0 U FZO1 SGDID:S000000383, Chr II from 589109-586542, reverse complement, Verified ORF, "Mitochondrial integral membrane protein involved in mitochondrial fusion and maintenance of the mitochondrial genome; contains N-terminal GTPase domain" * Jamie_FT_02_itms_16.12337.12337.3 2.6684 0.1267 90.5 2951.1543 2949.2463 10108.9 58 3.396 27.2 1 K.QWSLYSLFGKWT*PAFLDTISLS*VK.L Reverse_YGL014W 1 1 2.8% 888 97798 6.6 U PUF4 SGDID:S000002982, Chr VII from 466146-468812, Verified ORF, "Member of the PUF protein family, which is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding nucleolar ribosomal RNA-processing factors" * Jamie_FT_02_itms_39.26165.26165.2 1.2625 0.184 93.2 2861.8123 2860.8755 8879.8 3 3.772 20.8 1 K.ESVSNTPPIFSTS*SPT*NDFSISQTR.T YNL258C 1 1 2.8% 754 88072 5.0 U DSL1 SGDID:S000005202, Chr XIV from 160374-158110, reverse complement, Verified ORF, "Endoplasmic reticulum (ER)-localized peripheral membrane protein required for Golgi-to-ER retrograde traffic; component of the ER target site that interacts with coatomer, the major component of the COPI vesicle protein coat" * Jamie_FT_01_itms_34.20149.20149.2 2.1319 0.0408 92.6 2592.5723 2591.914 8148.9 11 3.071 30.0 1 K.EYMNLS*RIVSMIKNSIFIS*GK.E YLR214W 1 1 2.8% 686 78854 9.2 U FRE1 SGDID:S000004204, Chr XII from 568569-570629, Verified ORF, "Ferric reductase and cupric reductase, reduces siderophore-bound iron and oxidized copper prior to uptake by transporters; expression induced by low copper and iron levels" * Jamie_FT_01_itms_35.20948.20948.2 2.8344 0.2933 100.0 2166.2322 2166.6758 8679.2 17 4.897 33.3 1 R.RADLMAIALFPVVYLFGIR.N Reverse_YDL040C 1 1 2.7% 854 98905 8.8 U NAT1 SGDID:S000002198, Chr IV from 381435-378871, reverse complement, Verified ORF, "Subunit of the N-terminal acetyltransferase NatA (Nat1p, Ard1p, Nat5p); N-terminally acetylates many proteins, which influences multiple processes such as the cell cycle, heat-shock resistance, mating, sporulation, and telomeric silencing" * Jamie_FT_04_itms_35.32985.32985.4 2.904 0.1462 96.1 2637.8167 2633.529 10443.0 79 3.683 25.8 1 K.QS*SSNQGNNQIEDSNEDLSDSKR.K YIL107C 1 1 2.7% 827 93417 8.7 U PFK26 SGDID:S000001369, Chr IX from 165758-163275, reverse complement, Verified ORF, "6-phosphofructo-2-kinase, inhibited by phosphoenolpyruvate and sn-glycerol 3-phosphate, has negligible fructose-2,6-bisphosphatase activity, transcriptional regulation involves protein kinase A" * Jamie_FT_03_itms_20.20479.20479.2 2.0529 0.0486 92.6 2519.172 2517.628 7909.8 31 3.522 28.6 1 R.YSVIPTAPPSARS*S*FASDFLSR.K Reverse_YFL042C 1 1 2.7% 674 76347 5.0 U YFL042C SGDID:S000001852, Chr VI from 47744-45720, reverse complement, Uncharacterized ORF, "Due to a sequence change (deletion of G at 46151), YFL043C is now part of YFL042C." * Jamie_FT_01_itms_17.09843.09843.4 2.6938 0.1356 94.5 2011.7765 2007.1649 6720.2 258 3.743 31.4 1 K.EVQQLPS*GIEAITANSIR.R Reverse_YMR284W 1 1 2.7% 602 70648 6.4 U YKU70 SGDID:S000004897, Chr XIII from 838186-839994, Verified ORF, "Subunit of the telomeric Ku complex (Yku70p-Yku80p), involved in telomere length maintenance, structure and telomere position effect; relocates to sites of double-strand cleavage to promote nonhomologous end joining during DSB repair" * Jamie_FT_01_itms_30.17791.17791.2 1.7574 0.0717 90.0 1867.0521 1864.8773 5915.9 216 3.294 33.3 1 K.VSSPS*LTYLSPHS*NSK.L Reverse_YOR372C 1 1 2.7% 554 60523 9.5 U NDD1 SGDID:S000005899, Chr XV from 1036467-1034803, reverse complement, Verified ORF, "Transcriptional activator essential for nuclear division; localized to the nucleus; essential component of the mechanism that activates the expression of a set of late-S-phase-specific genes" * Jamie_FT_03_itms_41.34013.34013.3 2.4252 0.1291 91.8 1628.2144 1631.6536 7203.0 309 3.788 39.3 1 R.AQSKS*GSDSLLSQSR.K Reverse_YIL061C 1 1 2.7% 300 34447 10.0 U SNP1 SGDID:S000001323, Chr IX from 245556-244654, reverse complement, Verified ORF, "Component of U1 snRNP required for mRNA splicing via spliceosome; may interact with poly(A) polymerase to regulate polyadenylation; homolog of human U1 70K protein" * Jamie_FT_01_itms_12.06908.06908.2 1.9142 0.0362 93.0 1039.5322 1039.2657 3498.4 60 2.876 78.6 1 R.PKFYKVTR.G YGR094W 1 1 2.6% 1104 125770 7.0 U VAS1 SGDID:S000003326, Chr VII from 672190-675504, Verified ORF, "Mitochondrial and cytoplasmic valyl-tRNA synthetase" * Jamie_FT_01_itms_37.22110.22110.2 2.7024 0.3919 100.0 3432.672 3432.7668 8560.8 2 6.493 23.2 1 K.FTLEQDEDVLDTWFSSGLWPFSTLGWPEK.T Reverse_YBL037W 1 1 2.6% 1025 115011 8.6 U APL3 SGDID:S000000133, Chr II from 147212-150289, Verified ORF, "Alpha-adaptin, large subunit of the clathrin associated protein complex (AP-2); involved in vesicle mediated transport" * Jamie_FT_02_itms_18.13716.13716.2 1.638 0.1193 93.7 3193.7722 3191.4045 8637.2 129 3.271 19.2 1 K.LNITNTS*GGCKFHIALIPS*DEVDFPKR.I YLR347C 1 1 2.6% 861 94776 4.6 U KAP95 SGDID:S000004339, Chr XII from 826412-823827, reverse complement, Verified ORF, "Karyopherin beta, forms a dimeric complex with Srp1p (Kap60p) that mediates nuclear import of cargo proteins via a nuclear localization signal (NLS), interacts with nucleoporins to guide transport across the nuclear pore complex" * Jamie_FT_01_itms_39.23060.23060.2 3.0607 0.3493 100.0 2306.4722 2304.5586 11161.3 16 5.79 33.3 1 K.DSAFIEDDVFYAISALAASLGK.G Reverse_YJL141C 1 1 2.6% 807 91245 8.4 U YAK1 SGDID:S000003677, Chr X from 150308-147885, reverse complement, Verified ORF, "Serine-threonine protein kinase that is part of a glucose-sensing system involved in growth control in response to glucose availability; translocates from the cytoplasm to the nucleus and phosphorylates Pop2p in response to a glucose signal" * Jamie_FT_03_itms_18.19428.19428.2 1.7942 0.0794 92.1 2383.612 2381.7832 8158.4 4 3.518 30.0 1 R.LQKFPPQLPPSTSPPYASLRR.Y YJL130C 3 4 2.5% 2214 245123 5.9 U URA2 SGDID:S000003666, Chr X from 172285-165641, reverse complement, Verified ORF, "Bifunctional carbamoylphosphate synthetase (CPSase)-aspartate transcarbamylase (ATCase), catalyzes the first two enzymatic steps in the de novo biosynthesis of pyrimidines; both activities are subject to feedback inhibition by UTP" * Jamie_FT_01_itms_19.10985.10985.2 1.9619 0.1302 97.8 1256.3722 1256.5284 5317.3 231 4.248 50.0 1 K.VVGVNLIELATK.A * Jamie_FT_02_itms_23.16614.16614.2 2.0216 0.1069 96.8 2169.9521 2170.5725 9197.7 182 3.672 28.9 1 R.SIHITGVSNKEDLALIMTVK.A * Jamie_FT_01_itms_33.19272.19272.2 4.1946 0.4455 100.0 2495.112 2494.9316 11351.5 1 7.838 45.5 2 K.DSLPLLLAAVEEGKLTIDDIVLR.L YNR047W 1 1 2.5% 893 100546 8.0 U YNR047W SGDID:S000005330, Chr XIV from 708525-711206, Uncharacterized ORF, "Putative protein kinase that, when overexpressed, interferes with pheromone-induced growth arrest; localizes to the cytoplasm; potential Cdc28p substrate" * Jamie_FT_02_itms_27.19082.19082.2 1.8024 0.1528 97.6 2218.6921 2219.1106 10492.0 14 4.226 28.6 1 K.NASAHNS*SQSSLEGDSASSSSK.L Reverse_YDR138W 1 1 2.5% 752 87829 5.3 U HPR1 SGDID:S000002545, Chr IV from 730573-732831, Verified ORF, "Subunit of THO/TREX, related complexes that couple transcription elongation with mitotic recombination and elongation with mRNA metabolism and export, subunit of an RNA Pol II complex; regulates lifespan; similar to Top1p" * Jamie_FT_02_itms_35.24163.24163.2 1.4202 0.151 93.1 2380.912 2383.7996 11915.0 217 3.66 25.0 1 R.ADQLKKRHEQNIKYLLKEK.F Reverse_YER077C 1 1 2.5% 688 79548 9.4 U YER077C SGDID:S000000879, Chr V from 316596-314530, reverse complement, Uncharacterized ORF, "Hypothetical protein" * Jamie_FT_01_itms_26.15089.15089.2 1.8148 0.1318 96.5 2070.7722 2073.3628 7593.4 192 3.653 31.2 1 R.EKVQTKQLNTLSHFSRR.L YBR138C 1 1 2.5% 524 60459 7.9 U YBR138C SGDID:S000000342, Chr II from 515330-513756, reverse complement, Uncharacterized ORF, "Cytoplasmic protein of unknown function, potentially phosphorylated by Cdc28p; YBR138C is not an essential gene" * Jamie_FT_01_itms_22.12684.12684.2 1.7453 0.1598 97.7 1582.3322 1580.6512 8252.7 11 3.735 50.0 1 R.NRY*VSYGPSLDTK.N YJL138C 1 3 2.5% 395 44697 5.1 U TIF2 SGDID:S000003674, Chr X from 154609-153422, reverse complement, Verified ORF, "Translation initiation factor eIF4A, identical to Tif1p; DEA(D/H)-box RNA helicase that couples ATPase activity to RNA binding and unwinding; forms a dumbbell structure of two compact domains connected by a linker; interacts with eIF4G" YKR059W 1 3 2.5% 395 44697 5.1 U TIF1 SGDID:S000001767, Chr XI from 554629-555816, Verified ORF, "Translation initiation factor eIF4A, identical to Tif2p; DEA(D/H)-box RNA helicase that couples ATPase activity to RNA binding and unwinding; forms a dumbbell structure of two compact domains connected by a linker; interacts with eIF4G" Jamie_FT_01_itms_15.08672.08672.2 2.363 0.0374 96.8 1115.7522 1115.3585 8441.5 38 3.536 66.7 3 R.ILISTDLLAR.G Reverse_YER164W 1 1 2.4% 1468 168241 6.5 U CHD1 SGDID:S000000966, Chr V from 505387-509793, Verified ORF, "Nucleosome remodeling factor that functions in regulation of transcription elongation; contains a chromo domain, a helicase domain and a DNA-binding domain; component of both the SAGA and SILK complexes" * Jamie_FT_01_itms_28.16509.16509.4 3.648 0.0548 90.6 4193.7363 4193.627 7107.2 381 2.538 18.1 1 K.NQRNSFRTPIKVTSQKESQSKPKS*KSKS*KTRSKPK.S YMR205C 1 2 2.4% 959 104618 6.7 U PFK2 SGDID:S000004818, Chr XIII from 674765-671886, reverse complement, Verified ORF, "Beta subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes" * Jamie_FT_02_itms_17.13499.13499.3 3.6429 0.2568 100.0 2670.4744 2670.9812 6550.8 3 4.968 29.5 2 K.YEFDGLIIVGGFEAFESLHQLER.A Reverse_YLR188W 1 1 2.4% 695 75950 9.8 U MDL1 SGDID:S000004178, Chr XII from 528302-530389, Verified ORF, "Half-type ATP-binding cassette (ABC) transporter of the inner mitochondrial membrane, mediates export of peptides generated upon proteolysis of mitochondrial proteins, plays a role in the regulation of cellular resistance to oxidative stress" * Jamie_FT_01_itms_20.11883.11883.2 1.7104 0.1701 97.5 1866.3322 1866.0842 12918.1 26 4.03 37.5 1 R.FS*GTEVVSGHKGLVIVR.T YOR330C 1 1 2.3% 1254 143502 8.8 U MIP1 SGDID:S000005857, Chr XV from 943380-939616, reverse complement, Verified ORF, "Catalytic subunit of the mitochondrial DNA polymerase" * Jamie_FT_02_itms_24.17561.17561.3 3.1618 0.1909 98.4 3302.5144 3304.7917 11382.3 168 4.225 19.6 1 K.ADQRIRLPVNMPDYPTLHKIANDSAIPEK.Q YER110C 1 2 2.3% 1113 122601 4.6 U KAP123 SGDID:S000000912, Chr V from 382099-378758, reverse complement, Verified ORF, "Karyopherin beta, mediates nuclear import of ribosomal proteins prior to assembly into ribosomes and import of histones H3 and H4; localizes to the nuclear pore, nucleus, and cytoplasm; exhibits genetic interactions with RAI1" * Jamie_FT_02_itms_28.19963.19963.3 5.3005 0.4763 100.0 2874.3843 2875.3342 9424.6 1 8.778 32.0 2 K.NIVIYNYATVALDGLLEFIAYDAIAK.Y YLL032C 1 1 2.3% 825 94597 7.2 U YLL032C SGDID:S000003955, Chr XII from 76746-74269, reverse complement, Uncharacterized ORF, "Hypothetical protein" * Jamie_FT_02_itms_23.16704.16704.2 1.8461 0.1467 97.6 2150.5923 2152.2021 11504.4 8 3.954 33.3 1 K.S*RTTIDNTSQSGASPQRHK.M YML002W 1 1 2.3% 737 84602 6.7 U YML002W SGDID:S000004461, Chr XIII from 264541-266754, Uncharacterized ORF, "Hypothetical protein" * Jamie_FT_01_itms_36.21497.21497.2 1.7628 0.1142 94.3 2176.5322 2179.409 8691.0 2 3.478 37.5 1 K.IPENLFTDEASILY*WMR.I YHR149C 1 1 2.3% 734 81849 5.0 U SKG6 SGDID:S000001192, Chr VIII from 396662-394458, reverse complement, Uncharacterized ORF, "Protein of unknown function; found in the bud tip and bud neck, potential Cdc28p substrate; Skg6p interacts with Zds1p and Zds2p" * Jamie_FT_01_itms_11.06053.06053.4 2.9305 0.1324 96.3 1845.0565 1850.6287 4412.1 36 3.704 33.3 1 R.DDSDSSSS*SASS*TKNSK.S YJR053W 1 1 2.3% 574 66087 5.9 U BFA1 SGDID:S000003814, Chr X from 533941-535665, Verified ORF, "Component of the GTPase-activating Bfa1p-Bub2p complex involved in multiple cell cycle checkpoint pathways that control exit from mitosis" * Jamie_FT_01_itms_14.08295.08295.2 2.3035 0.1189 99.0 1516.1522 1515.768 8752.3 14 3.854 58.3 1 K.VIGNMILDEQNLR.W Reverse_YKL014C 1 1 2.2% 1764 203286 7.3 U URB1 SGDID:S000001497, Chr XI from 416556-411262, reverse complement, Verified ORF, "Nucleolar protein required for the normal accumulation of 25S and 5.8S rRNAs, associated with the 27SA2 pre-ribosomal particle; proposed to be involved in the biogenesis of the 60S ribosomal subunit" * Jamie_FT_01_itms_35.20917.20917.3 3.0657 0.1268 94.7 4478.724 4481.997 7406.0 54 3.74 16.4 1 K.NVPLRSINMLNKSPLASIYQFFSSSKMSSILSNSS*NT*KK.I YAL021C 1 1 2.2% 837 94670 6.9 U CCR4 SGDID:S000000019, Chr I from 113360-110847, reverse complement, Verified ORF, "Component of the CCR4-NOT transcriptional complex, which is involved in regulation of gene expression; component of the major cytoplasmic deadenylase, which is involved in mRNA poly(A) tail shortening" * Jamie_FT_03_itms_13.16754.16754.2 1.585 0.1134 92.4 2139.0522 2139.5027 7921.5 40 3.723 32.4 1 K.ILTEKSVTGLIFYLRDNR.P Reverse_YGR250C 1 1 2.2% 781 89513 5.4 U YGR250C SGDID:S000003482, Chr VII from 993526-991181, reverse complement, Uncharacterized ORF, "Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm" * Jamie_FT_01_itms_12.06992.06992.4 3.5618 0.0344 91.0 1962.6965 1962.8058 6574.4 42 3.228 35.4 1 K.NSEVSGEENFSVSS*S*SR.T YBL009W 1 1 2.2% 676 76372 9.4 U YBL009W SGDID:S000000105, Chr II from 207197-209227, Uncharacterized ORF, "haspin" * Jamie_FT_01_itms_26.15571.15571.2 2.0878 0.0391 91.6 1904.5322 1904.0494 7310.8 71 2.962 39.3 1 K.FWKNDNLLSRSLSS*R.S Reverse_YMR097C 1 1 2.2% 367 42144 9.5 U MTG1 SGDID:S000004703, Chr XIII from 460526-459423, reverse complement, Verified ORF, "Peripheral GTPase of the mitochondrial inner membrane, essential for respiratory competence, likely functions in assembly of the large ribosomal subunit, has homologs in plants and animals" * Jamie_FT_01_itms_13.07479.07479.2 2.2125 0.0166 95.5 959.2922 959.1337 5375.8 22 3.612 78.6 1 K.LLNRVDTK.N YDL171C 1 1 2.1% 2145 238100 6.6 U GLT1 SGDID:S000002330, Chr IV from 155641-149204, reverse complement, Verified ORF, "NAD(+)-dependent glutamate synthase (GOGAT), synthesizes glutamate from glutamine and alpha-ketoglutarate; with Gln1p, forms the secondary pathway for glutamate biosynthesis from ammonia; expression regulated by nitrogen source" * Jamie_FT_02_itms_27.19290.19290.4 3.4356 0.1368 97.1 4892.2563 4891.158 10086.4 2 3.757 17.0 1 K.FKSDENVIVGNTCFYGATSGT*AFISGSAGERFGVRNS*GATIVVER.I YGR204W 1 2 2.1% 946 102205 6.8 U ADE3 SGDID:S000003436, Chr VII from 905939-908779, Verified ORF, "Cytoplasmic trifunctional enzyme C1-tetrahydrofolate synthase, involved in single carbon metabolism and required for biosynthesis of purines, thymidylate, methionine, and histidine" * Jamie_FT_02_itms_21.15830.15830.2 3.6608 0.2924 100.0 2079.9922 2080.4878 10584.0 6 5.688 36.8 2 R.VTGFDITVASELMAILALSK.D YDR138W 1 1 2.1% 752 87829 5.3 U HPR1 SGDID:S000002545, Chr IV from 730573-732831, Verified ORF, "Subunit of THO/TREX, related complexes that couple transcription elongation with mitotic recombination and elongation with mRNA metabolism and export, subunit of an RNA Pol II complex; regulates lifespan; similar to Top1p" * Jamie_FT_01_itms_23.13749.13749.2 1.8352 0.1047 94.3 2056.632 2056.1997 9479.5 266 3.653 33.3 1 K.ERKKRALEEEAS*FPER.E Reverse_YBR302C 1 1 2.1% 379 45165 8.5 U COS2 SGDID:S000000506, Chr II from 811473-810334, reverse complement, Verified ORF, "Protein of unknown function, member of a family of conserved, often subtelomerically-encoded proteins" Reverse_YNL336W 1 1 2.1% 381 45288 8.5 U COS1 SGDID:S000005280, Chr XIV from 8330-9475, Verified ORF, "Protein of unknown function, member of a family of conserved, often subtelomerically-encoded proteins" Reverse_YML132W 1 1 2.1% 379 45165 8.5 U COS3 SGDID:S000004601, Chr XIII from 7244-8383, Verified ORF, "Protein involved in salt resistance; interacts with sodium:hydrogen antiporter Nha1p; member of a family of conserved, often subtelomerically-encoded proteins" Reverse_YJR161C 1 1 2.1% 383 45709 8.4 U COS5 SGDID:S000003922, Chr X from 743914-742763, reverse complement, Verified ORF, "Protein of unknown function, member of a family of conserved, often subtelomerically-encoded proteins" Reverse_YHL048W 1 1 2.1% 381 45880 8.3 U COS8 SGDID:S000001040, Chr VIII from 6400-7545, Verified ORF, "Nuclear membrane protein, member of a family of conserved, often subtelomerically-encoded proteins; regulation suggests a potential role in the unfolded protein response" Reverse_YFL062W 1 1 2.1% 379 45312 8.8 U COS4 SGDID:S000001832, Chr VI from 6426-7565, Verified ORF, "Protein of unknown function, member of a family of conserved, often subtelomerically-encoded proteins" Reverse_YDL248W 1 1 2.1% 383 45680 8.6 U COS7 SGDID:S000002407, Chr IV from 1802-2953, Verified ORF, "Protein of unknown function, member of a family of conserved, often subtelomerically-encoded proteins" Jamie_FT_01_itms_12.06905.06905.2 1.9257 0.0164 90.8 894.6322 895.0434 5926.6 10 2.694 78.6 1 K.FSLVDVSK.E YFL007W 1 1 2.0% 2143 245993 5.9 U BLM10 SGDID:S000001887, Chr VI from 123474-129905, Verified ORF, "Protein involved in nuclear assembly and or regulation of proteasomal core particles; required for normal resistance to bleomycin, may be involved in protection against oxidative damage" * Jamie_FT_02_itms_18.13573.13573.3 2.4671 0.1486 90.6 5181.534 5185.6074 8404.3 137 4.077 14.0 1 R.RFDKQHLS*FQWHVPSSDEITLSISILESLSEY*CINNVEELMK.A YLR067C 1 1 2.0% 965 112646 9.3 U PET309 SGDID:S000004057, Chr XII from 270711-267814, reverse complement, Verified ORF, "Specific translational activator for the COX1 mRNA, also influences stability of intron-containing COX1 primary transcripts; located in the mitochondrial inner membrane" * Jamie_FT_01_itms_31.18339.18339.2 1.7715 0.0795 91.5 2400.6921 2399.638 8528.8 28 3.007 33.3 1 K.KY*DITPSTAT*HTIMLKVYR.G Reverse_YPL150W 1 1 2.0% 901 100646 9.4 U YPL150W SGDID:S000006071, Chr XVI from 268187-270892, Uncharacterized ORF, "Hypothetical protein" * Jamie_FT_04_itms_3.11674.11674.2 1.6222 0.1341 94.2 2250.1921 2251.3125 8096.9 17 3.778 38.2 1 K.T*MCERT*FGFDTLKANGNK.D Reverse_YNR047W 1 1 2.0% 893 100546 8.0 U YNR047W SGDID:S000005330, Chr XIV from 708525-711206, Uncharacterized ORF, "Putative protein kinase that, when overexpressed, interferes with pheromone-induced growth arrest; localizes to the cytoplasm; potential Cdc28p substrate" * Jamie_FT_01_itms_22.12663.12663.3 2.8839 0.2026 98.7 2021.1843 2023.0348 8798.4 249 3.974 30.9 1 K.S*SGRSPSGNLTDKLS*LDK.P YOR144C 1 1 2.0% 791 91262 7.0 U ELG1 SGDID:S000005670, Chr XV from 605092-602717, reverse complement, Verified ORF, "Protein required for S phase progression and telomere homeostasis, forms an alternative replication factor C complex important for DNA replication and genome integrity; mutants are sensitive to DNA damage" * Jamie_FT_02_itms_14.11558.11558.2 1.7885 0.1062 94.3 2130.9521 2133.2388 12135.8 1 3.261 36.7 1 K.NWIETSFHT*LEKPT*LR.N YER041W 1 1 2.0% 759 87390 7.2 U YEN1 SGDID:S000000843, Chr V from 232460-234739, Verified ORF, "Protein of unknown function, has similarity to endonuclease Rth1p; potentially phosphorylated by Cdc28p" * Jamie_FT_01_itms_29.16913.16913.2 1.7682 0.0826 91.7 1816.6921 1818.8949 6713.8 7 2.983 39.3 1 R.SYT*TTGKAVINFIS*R.L Reverse_YNL118C 1 1 1.9% 970 108667 5.9 U DCP2 SGDID:S000005062, Chr XIV from 405566-402654, reverse complement, Verified ORF, "Protein required for the decapping of mRNAs, functions to allow the production of active Dcp1p, contains a pyrophosphatase MutT motif and several alpha-helical leucine-rich motifs" * Jamie_FT_01_itms_27.16064.16064.2 1.7748 0.1067 93.9 2023.7322 2021.9628 6098.0 5 3.77 35.3 1 K.GPGWEVSNS*EASANLNT*K.S Reverse_YGR288W 1 1 1.9% 473 54325 6.6 U MAL13 SGDID:S000003520, Chr VII from 1070299-1071720, Verified ORF, "MAL-activator protein, part of complex locus MAL1; nonfunctional in genomic reference strain S288C" * Jamie_FT_01_itms_15.08929.08929.2 1.8715 0.0748 94.7 995.0522 994.0953 5604.6 1 3.934 75.0 1 R.ANSIQAFSR.H YJL109C 1 1 1.8% 1769 200080 6.5 U UTP10 SGDID:S000003645, Chr X from 217226-211917, reverse complement, Verified ORF, "Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA" * Jamie_FT_01_itms_39.23028.23028.2 2.5322 0.1955 100.0 3381.2722 3380.9067 5642.0 1 4.316 25.0 1 K.VLGNVLQILPVDEFVNAVLPLLSTSTNEDIR.Y YER164W 1 1 1.8% 1468 168240 6.5 U CHD1 SGDID:S000000966, Chr V from 505387-509793, Verified ORF, "Nucleosome remodeling factor that functions in regulation of transcription elongation; contains a chromo domain, a helicase domain and a DNA-binding domain; component of both the SAGA and SILK complexes" * Jamie_FT_02_itms_20.14929.14929.2 1.9023 0.0732 92.8 2886.5923 2888.6792 11390.7 212 4.025 22.0 1 K.NSVNGDGT*AANSDSDDDSTSRSS*RRR.A Reverse_YDR038C 1 1 1.8% 1091 120296 5.5 U ENA5 SGDID:S000002445, Chr IV from 530693-527418, reverse complement, Verified ORF, "Protein with similarity to P-type ATPase sodium pumps" Reverse_YDR040C 1 1 1.8% 1091 120357 5.6 U ENA1 SGDID:S000002447, Chr IV from 538463-535188, reverse complement, Verified ORF, "P-type ATPase sodium pump, involved in Na+ and Li+ efflux to allow salt tolerance" Reverse_YDR039C 1 1 1.8% 1091 120317 5.5 U ENA2 SGDID:S000002446, Chr IV from 534578-531303, reverse complement, Verified ORF, "P-type ATPase sodium pump, involved in Na+ efflux to allow salt tolerance; likely not involved in Li+ efflux" Jamie_FT_01_itms_23.13649.13649.2 1.8617 0.0646 91.3 2250.7522 2252.4175 5935.9 3 3.585 34.2 1 K.VGDKGYWSSCCSIISEFAGK.G Reverse_YNL278W 1 1 1.8% 1060 118280 8.9 U CAF120 SGDID:S000005222, Chr XIV from 113271-116453, Verified ORF, "Part of the evolutionarily-conserved CCR4-NOT transcriptional regulatory complex involved in controlling mRNA initiation, elongation, and degradation" * Jamie_FT_02_itms_17.12971.12971.2 1.8853 0.0676 92.2 2174.152 2172.527 13643.9 57 3.091 30.6 1 K.KEFKVS*GVVKIITSSDILK.P Reverse_YLR305C 1 1 1.7% 1900 214606 7.5 U STT4 SGDID:S000004296, Chr XII from 743865-738163, reverse complement, Verified ORF, "Phosphatidylinositol-4-kinase that functions in the Pkc1p protein kinase pathway; required for normal vacuole morphology, cell wall integrity, and actin cytoskeleton organization" * Jamie_FT_02_itms_19.14226.14226.4 3.4465 0.0761 92.5 3979.8164 3978.417 9302.2 122 3.04 17.7 1 K.ASKHLRSLT*TARWSSSS*PVLITTKSHKLKFEYR.P Reverse_YLR223C 1 1 1.7% 1085 122491 4.7 U IFH1 SGDID:S000004213, Chr XII from 585492-582235, reverse complement, Verified ORF, "Essential protein with a highly acidic N-terminal domain; IFH1 exhibits genetic interactions with FHL1, overexpression interferes with silencing at telomeres and HM loci; potential Cdc28p substrate" * Jamie_FT_04_itms_11.16964.16964.2 1.5344 0.1307 92.9 2152.912 2150.363 8900.6 83 3.939 29.4 1 K.SLKEDNLMLPLS*TS*LSLK.P YNR011C 1 1 1.7% 876 99813 8.4 U PRP2 SGDID:S000005294, Chr XIV from 646952-644322, reverse complement, Verified ORF, "RNA-dependent ATPase in the DEAH-box family, required for activation of the spliceosome before the first transesterification step in RNA splicing" * Jamie_FT_01_itms_25.14988.14988.2 1.9531 0.0883 94.3 1932.0922 1933.0663 8613.7 8 3.461 42.9 1 K.LSKYS*CIMIDEAHER.T YDR266C 1 1 1.7% 639 72756 9.0 U YDR266C SGDID:S000002674, Chr IV from 1002017-1000098, reverse complement, Uncharacterized ORF, "Hypothetical protein" * Jamie_FT_01_itms_22.12761.12761.2 1.6351 0.1364 95.9 1227.7922 1229.2462 8220.1 59 3.38 55.0 1 R.ELNTTS*GGNKK.K YGL195W 2 2 1.6% 2672 296697 5.3 U GCN1 SGDID:S000003163, Chr VII from 131531-139549, Verified ORF, "Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn20p; proposed to stimulate Gcn2p activation by an uncharged tRNA" * Jamie_FT_01_itms_48.28597.28597.3 5.8331 0.3318 100.0 2687.6943 2687.1584 8839.8 1 6.661 35.2 1 K.SLVTSILLDILNLEPCLLENFIR.F * Jamie_FT_05_itms_8.17660.17660.2 1.8059 0.0738 91.4 2225.5723 2226.597 8618.5 21 3.119 33.3 1 K.SSKEVVRS*VSLQSMIILLR.K YGR184C 2 2 1.6% 1950 224836 5.5 U UBR1 SGDID:S000003416, Chr VII from 865758-859906, reverse complement, Verified ORF, "Ubiquitin-protein ligase (E3) that interacts with Rad6p/Ubc2p to ubiquitinate substrates of the N-end rule pathway; binds to the Rpn2p, Rpt1p, and Rpt6p proteins of the 19S particle of the 26S proteasome" * Jamie_FT_04_itms_8.15177.15177.2 1.6722 0.0879 90.7 1761.4521 1759.8705 7023.8 399 3.695 42.3 1 R.IISS*FLGNRSLT*YK.L * Jamie_FT_01_itms_36.21290.21290.2 2.2574 0.0901 97.6 1984.7522 1983.4347 8881.6 9 4.407 37.5 1 R.VAFMNPLQTMLSFLIEK.V Reverse_YAL029C 1 1 1.6% 1471 169343 7.6 U MYO4 SGDID:S000000027, Chr I from 92271-87856, reverse complement, Verified ORF, "One of two type V myosins; required for mother-specific HO expression, for the bud tip localization of ASH1 and IST2 mRNA; facilitates growth and orientation of ER tubules along with She3p" * Jamie_FT_01_itms_32.18672.18672.2 1.6974 0.1286 94.8 3064.6921 3065.5425 10747.0 210 2.986 21.7 1 K.QLVIY*RRRALQMRMASQVLIS*SRK.L Reverse_YDL195W 1 1 1.6% 1273 138703 5.7 U SEC31 SGDID:S000002354, Chr IV from 107209-111030, Verified ORF, "Essential phosphoprotein component (p150) of the COPII coat of secretory pathway vesicles, in complex with Sec13p; required for ER-derived transport vesicle formation" * Jamie_FT_01_itms_30.17833.17833.2 1.9998 0.0492 91.4 2343.5122 2343.4636 7236.6 17 3.356 31.6 1 K.FDKT*KLAESLTTNSELGS*IK.P YPR033C 1 1 1.6% 546 59953 7.7 U HTS1 SGDID:S000006237, Chr XVI from 639016-637376, reverse complement, Verified ORF, "Cytoplasmic and mitochondrial histidine tRNA synthetase; encoded by a single nuclear gene that specifies two messages; efficient mitochondrial localization requires both a presequence and an amino-terminal sequence " * Jamie_FT_01_itms_8.04718.04718.2 1.5765 0.1395 96.0 1044.5922 1044.9615 8431.4 264 3.869 56.2 1 K.GQSEET*ADK.I YMR093W 1 1 1.6% 513 57686 9.3 U UTP15 SGDID:S000004699, Chr XIII from 454014-455555, Verified ORF, "Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA" * Jamie_FT_01_itms_13.07405.07405.2 1.8277 0.0562 93.8 1059.9922 1059.1674 5569.2 162 3.368 71.4 1 R.QVIKT*FSR.F YEL041W 1 1 1.6% 495 55874 5.8 U YEL041W SGDID:S000000767, Chr V from 75944-77431, Uncharacterized ORF, "Hypothetical protein" * Jamie_FT_01_itms_14.08118.08118.2 2.0174 0.0589 96.3 1043.4521 1044.2389 4783.3 1 3.375 92.9 1 R.EVVEWILR.N Reverse_YFL016C 1 1 1.6% 511 55561 9.2 U MDJ1 SGDID:S000001878, Chr VI from 106230-104695, reverse complement, Verified ORF, "Protein involved in folding of mitochondrially synthesized proteins in the mitochondrial matrix; localizes to the mitochondrial inner membrane; member of the DnaJ family of molecular chaperones" * Jamie_FT_01_itms_10.05592.05592.2 2.2414 0.0367 97.1 1043.9521 1043.2137 7191.9 248 3.769 71.4 1 R.YLINNIHR.S YNR016C 2 3 1.5% 2233 250351 6.3 U ACC1 SGDID:S000005299, Chr XIV from 661376-654675, reverse complement, Verified ORF, "Acetyl-CoA carboxylase, biotin containing enzyme that catalyzes the carboxylation of acetyl-CoA to form malonyl-CoA; required for de novo biosynthesis of long-chain fatty acids" * Jamie_FT_02_itms_25.18101.18101.3 4.515 0.4106 100.0 3836.0344 3836.3794 12086.7 1 7.195 22.0 1 K.DLIDSNYVVFDVLLQFLTHQDPVVTAAAAQVYIR.R * Jamie_FT_02_itms_25.18132.18132.4 4.9625 0.1026 99.3 3836.3364 3836.3794 10212.9 10 4.197 20.2 2 K.DLIDSNYVVFDVLLQFLTHQDPVVTAAAAQVYIR.R YNL172W 1 1 1.5% 1748 196143 6.0 U APC1 SGDID:S000005116, Chr XIV from 310638-315884, Verified ORF, "Largest subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition" * Jamie_FT_01_itms_33.19481.19481.2 2.2292 0.0364 93.3 2987.3323 2990.3267 10095.4 90 2.872 24.0 1 R.MSDSIFAQLVSLLNVNDEMVADEEYR.L YOR153W 1 1 1.5% 1511 170437 7.8 U PDR5 SGDID:S000005679, Chr XV from 619840-624375, Verified ORF, "Short-lived membrane ABC (ATP-binding cassette) transporter, actively exports various drugs, expression regulated by Pdr1p; also involved in steroid transport, cation resistance, and cellular detoxification during exponential growth" * Jamie_FT_01_itms_39.23281.23281.2 2.2805 0.1316 99.0 2320.892 2318.7617 8534.3 60 3.724 31.0 1 R.VSPLTYFIQALLAVGVANVDVK.C YGL092W 1 1 1.5% 1317 145661 5.7 U NUP145 SGDID:S000003060, Chr VII from 337909-341862, Verified ORF, "Essential nucleoporin, catalyzes its own cleavage in vivo to generate a C-terminal fragment that assembles into the Nup84p subcomplex of the nuclear pore complex, and an N-terminal fragment of unknown function that is homologous to Nup100p" * Jamie_FT_01_itms_35.20426.20426.2 2.4074 0.0497 96.1 2382.4521 2380.7031 10005.7 177 3.36 28.9 1 R.NSSNEIEQIFLYLLLNDVVR.A YIL156W 1 1 1.5% 1071 123134 7.9 U UBP7 SGDID:S000001418, Chr IX from 48091-51306, Verified ORF, "Ubiquitin-specific protease that cleaves ubiquitin-protein fusions" * Jamie_FT_04_itms_5.13325.13325.2 1.4981 0.119 90.8 2024.4922 2027.3481 7269.6 281 3.125 30.0 1 K.MYENEMNMT*NVVLMVK.K YKL182W 1 2 1.4% 2051 228689 5.9 U FAS1 SGDID:S000001665, Chr XI from 100676-106831, Verified ORF, "Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities" * Jamie_FT_01_itms_33.19652.19652.2 3.4037 0.246 100.0 3070.152 3067.5493 5551.1 18 5.472 25.0 2 K.GYPIQFLTIGAGVPSLEVASEYIETLGLK.Y Reverse_YDR464W 1 1 1.4% 1435 161596 8.9 U SPP41 SGDID:S000002872, Chr IV from 1388862-1393169, Verified ORF, "Protein involved in negative regulation of expression of spliceosome components PRP4 and PRP3" * Jamie_FT_05_itms_3.13425.13425.2 1.1976 0.1833 92.0 2212.6921 2210.32 5160.0 108 4.44 21.1 1 K.KKKKST*SESAPS*KNATSAAK.S YLL003W 1 1 1.4% 946 112978 9.8 U SFI1 SGDID:S000003926, Chr XII from 143200-146040, Verified ORF, "Centrin (Cdc31p)-binding protein required for spindle pole body (SPB) duplication, localizes to the half-bridge of the SPB, required for progression through G(2)-M transition, has similarity to Xenopus laevis XCAP-C" * Jamie_FT_01_itms_19.11294.11294.2 2.341 0.146 99.5 1492.7922 1490.7393 8272.5 1 3.924 66.7 1 K.FIALSLAEYTYAK.N Reverse_YLR115W 1 1 1.4% 859 96254 7.6 U CFT2 SGDID:S000004105, Chr XII from 377986-380565, Verified ORF, "Subunit of the mRNA cleavage and polyadenlylation factor (CPF); required for pre-mRNA cleavage, polyadenylation and poly(A) site recognition, 43% similarity with the mammalian CPSF-100 protein." * Jamie_FT_01_itms_18.10449.10449.2 2.1498 0.3347 94.7 1303.6721 1303.5419 6417.9 63 5.285 50.0 1 T.LILTTKESNGVK.I Reverse_YER001W 1 1 1.4% 762 88529 7.2 U MNN1 SGDID:S000000803, Chr V from 153519-155807, Verified ORF, "Alpha-1,3-mannosyltransferase, integral membrane glycoprotein of the Golgi complex, required for addition of alpha1,3-mannose linkages to N-linked and O-linked oligosaccharides, one of five S. cerevisiae proteins of the MNN1 family" * Jamie_FT_01_itms_15.08766.08766.2 1.8031 0.0624 91.7 1365.0922 1364.587 8588.9 66 3.418 55.0 1 R.HNYSYPKLLTK.H YLR266C 1 1 1.4% 701 81274 6.8 U PDR8 SGDID:S000004256, Chr XII from 677726-675621, reverse complement, Verified ORF, "Transcription factor; targets include ATP-binding cassette (ABC) transporters, major facilitator superfamily transporters, and other genes involved in the pleiotropic drug resistance (PDR) phenomenon" * Jamie_FT_01_itms_15.08840.08840.2 1.7474 0.0638 91.5 1316.6122 1314.5022 8424.7 16 3.072 61.1 1 R.EIHKREMNEK.L Reverse_YHR045W 1 1 1.4% 560 62692 7.0 U YHR045W SGDID:S000001087, Chr VIII from 195544-197226, Uncharacterized ORF, "Hypothetical protein" * Jamie_FT_01_itms_12.07008.07008.2 1.6214 0.0704 90.7 1061.2722 1061.0966 5796.9 50 3.095 71.4 1 K.ELS*HPEIR.A Reverse_YOR165W 1 1 1.3% 776 89432 5.3 U SEY1 SGDID:S000005691, Chr XV from 644566-646896, Verified ORF, "Protein of unknown function, contains two predicted GTP-binding motifs GXXXXGKS and DXXG near the N-terminus, homolog of the Arabidopsis gene RHD3 (Root Hair Defective)" * Jamie_FT_01_itms_12.06680.06680.2 1.8119 0.1257 97.4 1185.3722 1184.3348 4068.6 2 3.552 72.2 1 K.EEDILQIAPR.D Reverse_YFL033C 1 1 1.2% 1770 196530 6.5 U RIM15 SGDID:S000001861, Chr VI from 74425-69113, reverse complement, Verified ORF, "Glucose-repressible protein kinase involved in signal transduction during cell proliferation in response to nutrients, specifically the establishment of stationary phase; originally identified as a regulator of IME2" * Jamie_FT_02_itms_25.18001.18001.2 2.0059 0.1322 97.7 2244.912 2243.3528 7651.6 161 3.16 27.5 1 R.S*VLEDGAGVSVVTCGLNELDK.T YDR093W 1 1 1.2% 1612 182617 6.2 U DNF2 SGDID:S000002500, Chr IV from 631277-636115, Verified ORF, "Non-essential P-type ATPase that is a potential aminophospholipid translocase, localizes to the plasma membrane and late exocytic or early endocytic membranes, likely involved in protein transport; potential Cdc28p substrate" * Jamie_FT_02_itms_22.16001.16001.2 2.0029 0.1556 98.9 2332.4321 2330.6133 8187.0 11 4.039 36.1 1 R.QS*LKCSKIIKSSRDITRTK.F YML072C 1 1 1.2% 1545 171075 7.1 U TCB3 SGDID:S000004537, Chr XIII from 129367-124730, reverse complement, Verified ORF, "Contains three calcium and lipid binding domains; localized to the bud; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; mRNA is targeted to the bud via the mRNA transport system involving She2p; C-terminal portion of Tcb1p, Tcb2p and Tcb3p interact" * Jamie_FT_01_itms_35.20670.20670.2 1.7224 0.0912 92.4 2228.2522 2226.241 9448.1 16 3.399 30.6 1 K.SQKANNDGVAVFDEECS*FK.A Reverse_YJL042W 1 1 1.2% 1398 155207 7.3 U MHP1 SGDID:S000003578, Chr X from 361165-365361, Verified ORF, "Microtubule-associated protein involved in assembly and stabilization of microtubules; overproduction results in cell cycle arrest at G2 phase; similar to Drosophila protein MAP and to mammalian MAP4 proteins" * Jamie_FT_01_itms_30.17556.17556.2 2.1037 0.1317 97.9 2038.6122 2038.1013 7682.4 59 3.718 34.4 1 R.RIAAGRDVHT*PS*PSREK.R YER114C 1 1 1.2% 1040 115688 8.8 U BOI2 SGDID:S000000916, Chr V from 393708-390586, reverse complement, Verified ORF, "Protein implicated in polar growth, functionally redundant with Boi1p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain" * Jamie_FT_01_itms_11.06452.06452.2 1.4891 0.193 97.6 1588.9722 1589.5759 7803.4 4 3.779 50.0 1 K.RSS*S*ASRTSSFKK.S Reverse_YMR266W 1 1 1.2% 953 107672 8.6 U YMR266W SGDID:S000004879, Chr XIII from 798517-801378, Uncharacterized ORF, "Membrane protein of unknown function; overexpression suppresses NaCl sensitivity of sro7 mutant" * Jamie_FT_01_itms_15.08797.08797.2 1.9973 0.0236 90.2 1328.2522 1328.6064 4753.4 29 3.297 55.0 1 K.AVKNIKHHPRK.K Reverse_YGL205W 1 1 1.2% 748 84042 8.5 U POX1 SGDID:S000003173, Chr VII from 108162-110408, Verified ORF, "Fatty-acyl coenzyme A oxidase, involved in the fatty acid beta-oxidation pathway; localized to the peroxisomal matrix" * Jamie_FT_01_itms_12.06866.06866.2 1.7755 0.0495 90.9 918.21216 917.9945 6810.3 248 2.743 68.8 1 K.VTVSGDPSR.V Reverse_YOL081W 1 1 1.1% 3079 351669 7.1 U IRA2 SGDID:S000005441, Chr XV from 171069-180308, Verified ORF, "GTPase-activating protein that negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, has similarity to Ira1p and human neurofibromin" * Jamie_FT_02_itms_37.24972.24972.4 2.7199 0.1815 96.4 4324.1367 4323.8975 10295.3 177 4.096 17.2 1 K.SHTYTHWKKY*IMRMSDLLIANPIT*AFRLINNRTR.R YPL242C 1 1 1.1% 1495 172830 8.9 U IQG1 SGDID:S000006163, Chr XVI from 95109-90622, reverse complement, Verified ORF, "Essential protein required for determination of budding pattern, promotes localization of axial markers Bud4p and Cdc12p and functionally interacts with Sec3p, localizes to the contractile ring during anaphase, member of the IQGAP family" * Jamie_FT_01_itms_12.06848.06848.2 1.5471 0.1767 96.8 1886.2922 1886.881 4040.7 221 4.18 28.1 1 K.S*LGT*NINTAPASPEEPK.E YBL079W 1 1 1.1% 1502 169474 6.1 U NUP170 SGDID:S000000175, Chr II from 75256-79764, Verified ORF, "Abundant subunit of the nuclear pore complex (NPC), required for proper localization of specific nucleoporins within the NPC, involved in nuclear envelope permeability and in chromosome segregation, has similarity to Nup157p" * Jamie_FT_01_itms_26.15242.15242.2 1.7309 0.0888 92.3 1701.5122 1699.6396 8537.9 2 3.049 50.0 1 K.KENSS*VTGTTATAGS*K.T YGL131C 1 1 1.1% 1403 163203 8.6 U SNT2 SGDID:S000003099, Chr VII from 265864-261653, reverse complement, Verified ORF, "DNA binding protein with similarity to the S. pombe Snt2 protein" * Jamie_FT_01_itms_19.10890.10890.2 3.0767 0.2698 100.0 1967.9722 1967.2291 7624.2 23 5.088 43.3 1 A.HNKLLEWELVQDSELI.I Reverse_YPR164W 1 1 1.1% 1407 161237 5.9 U MMS1 SGDID:S000006368, Chr XVI from 870699-874922, Verified ORF, "Protein likely involved in protection against replication-dependent DNA damage; mutants are sensitive to methyl methanesulfonate (MMS); implicated in regulation of Ty1 transposition" * Jamie_FT_02_itms_40.27272.27272.2 1.9729 0.0538 91.5 1816.8322 1818.9353 7646.4 206 3.297 35.7 1 R.S*NDLKYSDIIQNSIK.N YDR507C 1 1 1.1% 1142 129858 9.2 U GIN4 SGDID:S000002915, Chr IV from 1465776-1462348, reverse complement, Verified ORF, "Protein kinase involved in bud growth and assembly of the septin ring, proposed to have kinase-dependent and kinase-independent activities; undergoes autophosphorylation; similar to Kcc4p and Hsl1p" * Jamie_FT_01_itms_12.06761.06761.2 2.1304 0.0492 94.2 1228.0721 1229.4802 6052.9 211 3.244 59.1 1 -.MAINGNSIPAIK.D YAL019W 1 1 1.1% 1131 128507 5.4 U FUN30 SGDID:S000000017, Chr I from 114920-118315, Verified ORF, "Protein whose overexpression affects chromosome stability, potential Cdc28p substrate; homolog of Snf2p" * Jamie_FT_01_itms_12.06873.06873.3 2.8428 0.2336 100.0 1485.5944 1489.5015 6093.6 138 4.677 38.6 1 K.SNS*FNLQSARER.L YNL243W 1 1 1.1% 968 108996 5.5 U SLA2 SGDID:S000005187, Chr XIV from 188052-190958, Verified ORF, "Transmembrane actin-binding protein involved in membrane cytoskeleton assembly and cell polarization; adaptor protein that links actin to clathrin and endocytosis; present in the actin cortical patch of the emerging bud tip; dimer in vivo" * Jamie_FT_01_itms_20.11701.11701.2 2.3256 0.0385 96.0 1260.6522 1259.4886 8960.5 57 3.598 60.0 1 R.IDSDLQKALKK.A Reverse_YLL007C 1 2 1.1% 665 76622 5.9 U YLL007C SGDID:S000003930, Chr XII from 136298-134301, reverse complement, Uncharacterized ORF, "Hypothetical protein" * Jamie_FT_01_itms_16.09149.09149.2 1.8024 0.0712 95.2 833.5122 833.02136 2545.2 96 3.695 75.0 2 K.LAVNFIR.I Reverse_YML059C 1 1 1.0% 1679 187132 8.1 U NTE1 SGDID:S000004524, Chr XIII from 158258-153219, reverse complement, Verified ORF, "Serine esterase that deacylates exogenous lysophospholipids, homolog of human neuropathy target esterase (NTE); mammalian NTE1 deacylates phosphatidylcholine to glycerophosphocholine" * Jamie_FT_01_itms_53.31440.31440.2 1.207 0.173 90.5 1944.8722 1946.0813 7069.4 1 3.713 34.4 1 K.PELDASGSSKDVT*KFKR.I Reverse_YDR127W 1 1 1.0% 1588 174754 6.3 U ARO1 SGDID:S000002534, Chr IV from 704479-709245, Verified ORF, "Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids" * Jamie_FT_01_itms_18.10268.10268.2 2.9136 0.0142 96.9 1784.7322 1784.9236 10405.5 98 3.446 46.7 1 K.AGNVVNLFLSANS*ELR.T YGL094C 1 1 1.0% 1115 127039 6.7 U PAN2 SGDID:S000003062, Chr VII from 334468-331121, reverse complement, Verified ORF, "Essential subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex, which acts to control poly(A) tail length and regulate the stoichiometry and activity of postreplication repair complexes" * Jamie_FT_01_itms_11.06253.06253.2 1.7275 0.0969 93.8 1347.7322 1348.3317 6402.2 1 3.486 65.0 1 R.Y*RGHIGGNS*VK.D Reverse_YGR204W 1 1 1.0% 946 102205 6.8 U ADE3 SGDID:S000003436, Chr VII from 905939-908779, Verified ORF, "Cytoplasmic trifunctional enzyme C1-tetrahydrofolate synthase, involved in single carbon metabolism and required for biosynthesis of purines, thymidylate, methionine, and histidine" * Jamie_FT_01_itms_12.06961.06961.2 2.0104 0.027 92.6 1045.3121 1045.2272 7248.1 64 3.001 75.0 1 K.EALVRSTIR.D YOR328W 1 1 0.8% 1564 176460 7.9 U PDR10 SGDID:S000005855, Chr XV from 931798-936492, Verified ORF, "ABC (ATP-binding cassette) membrane pump involved in the pleiotropic drug resistance network, regulated by Pdr1p and Pdr3p, similar to Pdr5p" * Jamie_FT_01_itms_15.08949.08949.2 1.9755 0.086 95.6 1501.3922 1502.5754 7186.6 281 3.314 50.0 1 R.TLT*SQSSLLSQEK.R Reverse_YGL140C 1 1 0.7% 1219 137476 8.1 U YGL140C SGDID:S000003108, Chr VII from 245017-241358, reverse complement, Uncharacterized ORF, "Hypothetical protein" * Jamie_FT_01_itms_10.05469.05469.2 1.6349 0.1142 94.3 978.77216 981.13983 5258.3 450 3.698 62.5 1 R.TLGKYATAR.A YHR119W 1 1 0.7% 1080 123912 9.0 U SET1 SGDID:S000001161, Chr VIII from 346046-349288, Verified ORF, "Histone methyltransferase, subunit of the COMPASS (Set1C) complex which methylates Rad6p ubiquitinated histone H3 on lysine 4; required in transcriptional silencing near telomeres and at the silent mating type loci; contains a SET domain" * Jamie_FT_01_itms_7.04043.04043.2 1.229 0.166 92.4 1058.6122 1056.9811 6143.5 108 3.592 50.0 1 R.Y*NHNDGTR.R Reverse_YDR150W 1 1 0.6% 2748 313032 5.4 U NUM1 SGDID:S000002557, Chr IV from 755623-763869, Verified ORF, "Protein required for nuclear migration, localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex" * Jamie_FT_01_itms_23.13321.13321.2 1.7803 0.084 92.4 2010.0721 2009.3281 9083.0 298 3.058 31.2 1 K.QLLNKYSDNSLLIMNNK.E Reverse_YKL129C 1 1 0.6% 1271 142507 9.4 U MYO3 SGDID:S000001612, Chr XI from 200163-196348, reverse complement, Verified ORF, "One of two type I myosins; localizes to actin cortical patches; deletion of MYO3 has little effect on growth, but myo3 myo5 double deletion causes severe defects in growth and actin cytoskeleton organization" * Jamie_FT_01_itms_12.07160.07160.2 2.008 0.0557 95.9 893.33215 894.06165 4314.0 138 3.614 78.6 1 K.VYKNGGKK.E Proteins Peptide IDs Spectra Unfiltered 11789 233739 289814 Filtered 400 688 1386 Forward matches 277 562 1259 Decoy matches 123 126 127 Forward FP rate 44.4 22.42 10.09 Classification Nonredundant Proteins Redundant Proteins Unclassified 0 0