Protein Overview: PUS6

General Information:
Name(s) found:	PUS6 / YGR169C [SGD]
Description(s) found: Found 27 descriptions. SHOW ALL	tRNA:pseudouridine synthase, catalyzes the conversion of uridine to pseudouridine at position 31 in cytoplasmic and mitochondrial tRNAs; mutation of Asp168 to Ala abolishes enzyme activity; not essential for viability [SGD]
Organism:	Saccharomyces cerevisiae
Length:	404 amino acids
Gene Ontology:
Cellular Component:	cytoplasm [IDA] mitochondrion [IDA]
Biological Process:	pseudouridine synthesis [IDA] tRNA modification [IDA]
Molecular Function:	pseudouridylate synthase activity [IDA]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSTIKVIEVY TQNGLRKVRP YYNRRSAFVK GRWLGKLLID VLVSEFKLRP RAYYLDQIRK  60
   61 GTYRLIRDGV PLVPDHLMTT IIKNHDVLET TTHKHEPPVK QWCSQEVEAE DLPGRIAGFN 120
  121 IVFEDESILV IDKPSGIPVH PTGQFYQNTI TELLKLHGVD ALPCYRLDKI TSGLLILAKN 180
  181 SQSAGEIQKS IRSRDMIKIY LARVKGRFPH SELILDNENA AETTFEDTSK VTVEMTPIYS 240
  241 IDPKRQFPVG LSTSKDAITK FYPIRYFSHA DETVVACKPI TGRTHQIRIH LARLGHPIVN 300
  301 DSVYCSHITK YPERLKFITQ FPRWENQQDL DAEELKVRFQ KFVDETKNNC RTMETFCPEC 360
  361 HTVDLKDPVL SDLELWLHAW KYEEINGKFK FKTDLPKWAQ LDNS

Protein Complex Data

No complex found for this protein.

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	PUS6	GIP1	2	Unpublished Fields Lab Data
View Screen	PUS6	ENT2	2	Unpublished Fields Lab Data

Microscopy / Localization Data

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No microscopy data found in the PDR for this protein.

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..112]	PSI-BLAST	27.0	Ribosomal small subunit pseudouridine 516 synthase RsuA; Pseudouridine synthase RsuA N-terminal domain
2	View Details	[113..404]	PSI-BLAST	27.0	Ribosomal small subunit pseudouridine 516 synthase RsuA; Pseudouridine synthase RsuA N-terminal domain

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
pseudouridine synthase activity	5.19957135839682	bayes_pls_golite062009
intramolecular transferase activity	3.78688422405974	bayes_pls_golite062009
isomerase activity	2.91720316078381	bayes_pls_golite062009
catalytic activity	2.02180970970004	bayes_pls_golite062009
transcription regulator activity	1.21173188039059	bayes_pls_golite062009
DNA binding	0.950713703876672	bayes_pls_golite062009
nucleic acid binding	0.909704702595901	bayes_pls_golite062009
binding	0.730744558777463	bayes_pls_golite062009
RNA binding	0.706879359688019	bayes_pls_golite062009
transferase activity	0.237728328252954	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2