Protein Overview: Roc2-PA, Roc2-PB

General Information:
Name(s) found:	Roc2-PB / FBpp0291421 [FlyBase] Roc2-PA / FBpp0087189 [FlyBase]
Description(s) found: SHOW ONLY BEST	gi\|24652785\|ref\|NP_610691.1\| Roc2 [Drosophila melanogaster] [NCBI NR] gi\|225193076\|gb\|ACN81331.1\| MIP07211p [Drosophila melanogaster] [NCBI NR] gi\|21627443\|gb\|AAF58633.2\| Roc2 [Drosophila melanogaster] [NCBI NR] gi\|21064639\|gb\|AAM29549.1\| RE61847p [Drosophila melanogaster] [NCBI NR] The gene Roc2 is referred to in FlyBase by the symbol Dmel\Roc2 (CG8998, FBgn0044020). It is a protein_coding_gene from Drosophila melanogaster. Its sequence location is 2R:7540261..7566857. It has the cytological map location 48B4-48C1. There is experimental evidence that it has the molecular function</span>: ubiquitin-protein ligase activity. There is experimental evidence that it is involved in the biological process</span>: protein ubiquitination. 24 alleles are reported. No phenotypic data is available. It has 2 annotated transcripts and 2 annotated polypeptides. [FlyBase] >CG8998-PA translation from_gene[CG8998 CG8998 FBgn0033641] seq_release:3 mol_weight=13101 cds_boundaries:(2R:6,717,618..6,743,078[-]) aa_length:113 transcript_info:[CG8998-RA seq_release:3] gene_info:[gene symbol:CG8998 FBgn0033641 gene_boundaries:(2R [hmr_yeast5_DM3-1_pombe.fasta] (Q9V607) CG8998 protein (RE61847p) [DrosMelano7227.FASTAC.txt] CG8998-PA [Drosophila melanogaster]gi\|21064639\|gb\|AAM29549.1\| RE61847p [Drosophila melanogaster]gi\|24652785\|ref\|NP_610691.1\| CG8998-PA [Drosophila melanogaster] [nrdb_11152004_Chlamydomonas] type=protein; loc=2R:complement(7540407..7540588,7565711..7565870); ID=FBpp0087189; name=Roc2-PA; parent=FBgn0044020,FBtr0088085; dbxref=FlyBase:FBpp0087189,FlyBase_Annotation_IDs:CG8998-PA,GB_protein:AAF58633.2,REFSEQ:NP_610691,GB_protein:AAF58633; MD5=2 [fly-100309-contam.fasta] Roc2 CG8998-PA [Drosophila melanogaster] [nr-271106-contam.fasta] RE61847p [Drosophila melanogaster] [nr-271106-contam.fasta] CG8998-PA [Drosophila melanogaster] [nr-271106-contam.fasta] type=protein; loc=2R:complement(7540407..7540588,7565711..7565870); ID=FBpp0087189; name=Roc2-PA; parent=FBgn0044020,FBtr0088085; dbxref=FlyBase:FBpp0087189,FlyBase_Annotation_IDs:CG8998-PA,GB_protein:AAF58633.2,REFSEQ:NP_610691,GB_protein:AAF58633; MD5=2a374b041a492d3ded47b63435147288; length=113; release=r5.16; species=Dmel; [fly-contam-His-TEV-Tub4complex.fasta] type=protein; loc=2R:complement(7540407..7540588,7565711..7565870); ID=FBpp0087189; name=Roc2-PA; parent=FBgn0044020,FBtr0088085; dbxref=FlyBase:FBpp0087189,FlyBase_Annotation_IDs:CG8998-PA,GB_protein:AAF58633.2,REFSEQ:NP_610691,GB_protein:AAF58633; MD5=2a374b041a492d3ded47b63435147288; length=113; release=r5.21; species=Dmel; [Other_drosophila_na_09-09-2009_reversed.fasta] type=protein; loc=2R:complement(7540407..7540588,7565711..7565870); ID=FBpp0291421; name=Roc2-PB; parent=FBgn0044020,FBtr0302211; dbxref=FlyBase:FBpp0291421,FlyBase_Annotation_IDs:CG8998-PB; MD5=2a374b041a492d3ded47b63435147288; length=113; release=r5.21; species=Dmel; [Other_drosophila_na_09-09-2009_reversed.fasta] MIP07211p OS=Drosophila melanogaster GN=Roc2 PE=1 SV=1 [UniProt_drosophila_07-01-2011_reversed.fasta] type=protein; loc=2R:complement(7540407..7540588,7565711..7565870); ID=FBpp0087189; name=Roc2-PA; parent=FBgn0044020,FBtr0088085; dbxref=FlyBase:FBpp0087189,FlyBase_Annotation_IDs:CG8998-PA,GB_protein:AAF58633.2,REFSEQ:NP_610691,GB_protein:AAF58633,FlyMine:FBpp0087189,modMine:FBpp0087189; MD5=2a374b041a492d3ded47b63435147288; length=113; release=r5.46; species=Dmel; [fly-062812-sfps-sandra-contam.fasta] type=protein; loc=2R:complement(7540407..7540588,7565711..7565870); ID=FBpp0291421; name=Roc2-PB; parent=FBgn0044020,FBtr0302211; dbxref=REFSEQ:NP_001163119,GB_protein:ACZ94394,FlyBase:FBpp0291421,FlyBase_Annotation_IDs:CG8998-PB; MD5=2a374b041a492d3ded47b63435147288; length=113; release=r5.46; species=Dmel; [fly-062812-sfps-sandra-contam.fasta] MIP07211p OS=Drosophila melanogaster GN=Roc2 PE=4 SV=1 [UniProt_D_Melanogaster_07132012_07-13-2012_reversed.fasta] type=protein; loc=2R:complement(join(11678206..11678365,11652902..11653083)); ID=FBpp0087189; name=Roc2-PA; parent=FBgn0044020,FBtr0088085; dbxref=FlyBase:FBpp0087189,FlyBase_Annotation_IDs:CG8998-PA,GB_protein:AAF58633.2,REFSEQ:NP_610691,GB_protein:AAF58633,FlyMine:FBpp0087189,modMine:FBpp0087189; MD5=2a374b041a492d3ded47b63435147288; length=113; release=r6.02; species=Dmel; [fly-2014-05-contam.fasta] type=protein; loc=2R:complement(join(11678206..11678365,11652902..11653083)); ID=FBpp0291421; name=Roc2-PB; parent=FBgn0044020,FBtr0302211; dbxref=REFSEQ:NP_001163119,GB_protein:ACZ94394,FlyBase:FBpp0291421,FlyBase_Annotation_IDs:CG8998-PB; MD5=2a374b041a492d3ded47b63435147288; length=113; release=r6.02; species=Dmel; [fly-2014-05-contam.fasta] type=protein; loc=2R:complement(join(11678206..11678365,11652902..11653083)); ID=FBpp0087189; name=Roc2-PA; parent=FBgn0044020,FBtr0088085; dbxref=FlyBase:FBpp0087189,FlyBase_Annotation_IDs:CG8998-PA,GB_protein:AAF58633.2,REFSEQ:NP_610691,GB_protein:AAF58633,FlyMine:FBpp0087189,modMine:FBpp0087189; MD5=2a374b041a492d3ded47b63435147288; length=113; release=r6.07; species=Dmel; [fly-08202015-contam.fasta] type=protein; loc=2R:complement(join(11678206..11678365,11652902..11653083)); ID=FBpp0291421; name=Roc2-PB; parent=FBgn0044020,FBtr0302211; dbxref=REFSEQ:NP_001163119,GB_protein:ACZ94394,FlyBase:FBpp0291421,FlyBase_Annotation_IDs:CG8998-PB; MD5=2a374b041a492d3ded47b63435147288; length=113; release=r6.07; species=Dmel; [fly-08202015-contam.fasta] FBpp0087189 type=protein; loc=2R:complement(join(11678206..11678365,11652902..11653083)); ID=FBpp0087189; name=Roc2-PA; parent=FBgn0044020,FBtr0088085; dbxref=FlyBase:FBpp0087189,FlyBase_Annotation_IDs:CG8998-PA,GB_protein:AAF58633.2,REFSEQ:NP_610691,GB_protein:AAF58633,FlyMine:FBpp0087189,modMine:FBpp0087189; MD5=2a374b041a492d3ded47b63435147288; length=113; release=r6.09; species=Dmel; [dmel-all-translation-r6.09.fasta] FBpp0291421 type=protein; loc=2R:complement(join(11678206..11678365,11652902..11653083)); ID=FBpp0291421; name=Roc2-PB; parent=FBgn0044020,FBtr0302211; dbxref=REFSEQ:NP_001163119,GB_protein:ACZ94394,FlyBase:FBpp0291421,FlyBase_Annotation_IDs:CG8998-PB; MD5=2a374b041a492d3ded47b63435147288; length=113; release=r6.09; species=Dmel; [dmel-all-translation-r6.09.fasta] [fly-122215-dmel-6-09-contam.fasta] type=protein; loc=2R:complement(join(11678206..11678365,11652902..11653083)); ID=FBpp0087189; name=Roc2-PA; parent=FBgn0044020,FBtr0088085; dbxref=FlyBase:FBpp0087189,FlyBase_Annotation_IDs:CG8998-PA,GB_protein:AAF58633.2,REFSEQ:NP_610691,GB_protein:AAF58633,UniProt/TrEMBL:Q7JWH5,FlyMine:FBpp0087189,modMine:FBpp0087189; MD5=2a374b041a492d3ded47b63435147288; length=113; release=r6.14; species=Dmel; [fly-dmel-jan2017-sandracontam.fasta] type=protein; loc=2R:complement(join(11678206..11678365,11652902..11653083)); ID=FBpp0291421; name=Roc2-PB; parent=FBgn0044020,FBtr0302211; dbxref=REFSEQ:NP_001163119,GB_protein:ACZ94394,FlyBase:FBpp0291421,FlyBase_Annotation_IDs:CG8998-PB,UniProt/TrEMBL:Q7JWH5; MD5=2a374b041a492d3ded47b63435147288; length=113; release=r6.14; species=Dmel; [fly-dmel-jan2017-sandracontam.fasta]
Organism:	Drosophila melanogaster
Length:	113 amino acids
Gene Ontology:
Cellular Component:	SCF ubiquitin ligase complex [NAS]
Biological Process:	protein ubiquitination [IDA]
Molecular Function:	ubiquitin-protein ligase activity [IDA][ISS] zinc ion binding [IEA] protein binding [IEA]

Sequence:
Sequence: [PDR BLAST] [ProtParam]	1 11 21 31 41 51 \| \| \| \| \| \| 1 MADDPENSVD RPTDDGDAGK PEKMFTLKKW NAVAMWSWDV ECDICAICRV QVMDSCLRCQ 60 61 ADNKRDVMGR QDCVVVWGEC NHSFHHCCMS LWVKQNNRCP LCQQEWSIQR MGK

Protein Complex Data

No complex found for this protein.

Mass Spectrometry Data

No mass spectrometry results found for this protein.

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

No yeast two-hybrid interactions found for this protein.

Microscopy / Localization Data

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No microscopy data found in the PDR for this protein.

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..113]	PSI-BLAST	19.522879	Crystal Structure of The Cand1-Cul1-Roc1 Complex

Functions predicted (by domain):

#

Gene Ontology predictions

1

Term	Confidence	Notes
ubiquitin-protein ligase activity	10.2497392096259	bayes_pls_golite062009
small conjugating protein ligase activity	10.0460587929138	bayes_pls_golite062009
acid-amino acid ligase activity	8.99384576623969	bayes_pls_golite062009
ligase activity, forming carbon-nitrogen bonds	7.6988425139962	bayes_pls_golite062009
NEDD8 ligase activity	5.61847630780351	bayes_pls_golite062009
ligase activity	3.92677358871643	bayes_pls_golite062009
binding	2.78710909602478	bayes_pls_golite062009
protein binding	1.5975811408548	bayes_pls_golite062009
small conjugating protein-specific protease activity	1.2126425713931	bayes_pls_golite062009
ubiquitin-specific protease activity	1.03469202541773	bayes_pls_golite062009
transcription regulator activity	0.943706743211193	bayes_pls_golite062009
transcription factor binding	0.90065892745057	bayes_pls_golite062009
nucleic acid binding	0.708499177773075	bayes_pls_golite062009
hydrolase activity	0.52386813728704	bayes_pls_golite062009
cysteine-type peptidase activity	0.50197530717959	bayes_pls_golite062009
transcription repressor activity	0.393708074680332	bayes_pls_golite062009
DNA binding	0.365874679056682	bayes_pls_golite062009
ubiquitin thiolesterase activity	0.265769062717221	bayes_pls_golite062009
catalytic activity	0.14112008370434	bayes_pls_golite062009

Reference: Drew K, et al. (2011) The proteome folding project: Proteome-scale prediction of structure and function. Genome Res. 2011 Sep 16

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

YRC Informatics Platform - Version 3.0
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