Protein Overview: FOL1

General Information:
Name(s) found:	FOL1 / YNL256W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|73920209\|sp\|P53848.2\|FOL1_YEAST RecName: Full=Folic acid synthesis protein FOL1; Includes: RecName: Full=Dihydroneopterin aldolase; Short=DHNA; AltName: Full=FASA; AltName: Full=FASB; Includes: RecName: Full=2-amino-4-hydroxy-6-hydroxymethyldihydropter [NCBI NR] gi\|37362687\|ref\|NP_014143.2\| Multifunctional enzyme of the folic acid biosynthesis pathway, has dihydropteroate synthetase, dihydro-6-hydroxymethylpterin pyrophosphokinase, and dihydroneopterin aldolase activities; Fol1p [Saccharomyces cerevisiae] [NCBI NR] Folic acid synthesis protein FOL1 OS=Saccharomyces cerevisiae GN=FOL1 PE=1 SV=2 [Swiss-Prot] Folic acid synthesis protein FOL1 [Includes: Dihydroneopterin aldolase - Saccharomyces cerevisiae (Baker's yeast) [Swiss-Prot] FOL1 Dihydroneopterin aldolase, dihydro-6-hydroxymethylpterin pyrophosphokinase, dihydropteroate synthetase [mips] Multifunctional enzyme of the folic acid biosynthesis pathway, has dihydropteroate synthetase, dihydro-6-hydroxymethylpterin pyrophosphokinase, and dihydroneopterin aldolase activities [SGD] Chr XIV from 164622-167096 [yeastorf5.fasta] FOL1 SGDID:S0005200, Chr XIV from 164622-167096, Verified ORF [yeastflysequest.fasta] Fol1p [Saccharomyces cerevisiae] [nrdb_11152004_Chlamydomonas] FOL1 SGDID:S000005200, Chr XIV from 164624-167098, Verified ORF, "Multifunctional enzyme of the folic acid biosynthesis pathway, has dihydropteroate synthetase, dihydro-6-hydroxymethylpterin pyrophosphokinase, and dihydroneopterin aldolase activities" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] Multifunctional enzyme of the folic acid biosynthesis pathway, has dihydropteroate synthetase, dihydro-6-hydroxymethylpterin pyrophosphokinase, and dihydroneopterin aldolase activities; Fol1p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Folic acid synthesis protein FOL1 [Includes: Dihydroneopterin aldolase (DHNA) (FASA) (FASB); 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase (7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase) (HPPK) (6-hydroxymethyl-7,8-dihydropteri [nr-271106-contam.fasta] [chemostatdb.fasta] Folic acid synthesis protein FOL1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=FOL1 PE=1 SV=2 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] FOL1 SGDID:S000005200, Chr XIV from 164623-167097, Genome Release 64-1-1, Verified ORF, "Multifunctional enzyme of the folic acid biosynthesis pathway, has dihydropteroate synthetase, dihydro-6-hydroxymethylpterin pyrophosphokinase, and dihydroneopterin aldolase activities" [yeast-030211-contam.fasta] FOL1 SGDID:S000005200, Chr XIV from 164623-167097, Genome Release 64-2-1, Verified ORF, "Multifunctional enzyme of the folic acid biosynthesis pathway; has dihydropteroate synthetase, dihydro-6-hydroxymethylpterin pyrophosphokinase, and dihydroneopterin aldolase activities" [20141208_orf_trans.fasta] YNL256W SGDID:S000005200, Chr XIV from 164623-167097, Genome Release 64-1-1, Verified ORF, "Multifunctional enzyme of the folic acid biosynthesis pathway, has dihydropteroate synthetase, dihydro-6-hydroxymethylpterin pyrophosphokinase, and dihydroneopterin aldolase activities" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	824 amino acids
Gene Ontology:
Cellular Component:	cytoplasm [IDA] mitochondrion [IDA ] mitochondrial envelope [IDA]
Biological Process:	folic acid and derivative biosynthetic process [IMP ]
Molecular Function:	2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase activity [IDA][ISS] dihydroneopterin aldolase activity [IDA][ISS] dihydropteroate synthase activity [IDA][IMP]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSKLFSTVNS ARHSVPLGGM RDYVHIKKLE MNTVLGPDSW NQLMPQKCLL SLDMGTDFSK  60
   61 SAATDDLKYS LNYAVISRDL TNFVSKKKNW GSVSNLAKSV SQFVMDKYSG VECLNLEVQA 120
  121 DTTHIRSDHI SCIIQQERGN PESQEFDVVR ISELKMLTLI GVFTFERLKK QYVTLDIKLP 180
  181 WPKKAELPPP VQSIIDNVVK FVEESNFKTV EALVESVSAV IAHNEYFQKF PDSPLVVKVL 240
  241 KLNAITATEG VGVSCIREPR EIAMVNIPYL SSIHESSDIK FQLSSSQNTP IEGKNTWKRA 300
  301 FLAFGSNIGD RFKHIQMALQ LLSREKTVKL RNISSIFESE PMYFKDQTPF MNGCVEVETL 360
  361 LTPSELLKLC KKIEYEELQR VKHFDNGPRT IDLDIVMFLN SAGEDIIVNE PDLNIPHPRM 420
  421 LERTFVLEPL CELISPVHLH PVTAEPIVDH LKQLYDKQHD EDTLWKLVPL PYRSGVEPRF 480
  481 LKFKTATKLD EFTGETNRIT VSPTYIMAIF NATPDSFSDG GEHFADIESQ LNDIIKLCKD 540
  541 ALYLHESVII DVGGCSTRPN SIQASEEEEI RRSIPLIKAI RESTELPQDK VILSIDTYRS 600
  601 NVAKEAIKVG VDIINDISGG LFDSNMFAVI AENPEICYIL SHTRGDISTM NRLAHYENFA 660
  661 LGDSIQQEFV HNTDIQQLDD LKDKTVLIRN VGQEIGERYI KAIDNGVKRW QILIDPGLGF 720
  721 AKTWKQNLQI IRHIPILKNY SFTMNSNNSQ VYVNLRNMPV LLGPSRKKFI GHITKDVDAK 780
  781 QRDFATGAVV ASCIGFGSDM VRVHDVKNCS KSIKLADAIY KGLE

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		FOL1 SSE1
View Details	Krogan NJ, et al. (2006)		FOL1 URA3 YMR074C

Mass Spectrometry Data

No mass spectrometry results found for this protein.

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	NAF1	FOL1	1	Hazbun TR, et al. (2003)

Microscopy / Localization Data

New Feature: Upload Your Own Microscopy Data

	PROTEIN(S)	PUBLICATION
View Data	FOL1	Huh WK, et al. (2003)

Protein Structure Data

[What does the above image mean?]
[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..140]	PSI-BLAST	33.39794	Crystal structure of 7,8-dihydroneopterin aldolase in complex with guanine
2	View Details	[141..295]	PSI-BLAST	62.69897	No description for 2cg8A was found.
3	View Details	[296..824]	PSI-BLAST	171.0	X-ray structure of the bifunctional 6-hydroxymethyl-7,8- dihydroxypterin pyrophosphokinase dihydropteroate synthase from Sacchromyces cerevisiae

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
GTP cyclohydrolase I activity	4.1430878480831	bayes_pls_golite062009
cyclohydrolase activity	3.66553359909123	bayes_pls_golite062009
GTP cyclohydrolase activity	3.44912994722205	bayes_pls_golite062009
6-pyruvoyltetrahydropterin synthase activity	2.77658228107642	bayes_pls_golite062009
dihydroneopterin aldolase activity	2.51107306055654	bayes_pls_golite062009
catalytic activity	2.20788162511962	bayes_pls_golite062009
hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amidines	1.73101699306211	bayes_pls_golite062009
hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds	0.974891318710762	bayes_pls_golite062009
binding	0.641976427105342	bayes_pls_golite062009
oxidoreductase activity	0.0783540825826956	bayes_pls_golite062009
protein binding	0.045916344848404	bayes_pls_golite062009
urate oxidase activity	0.0294957696532921	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.98

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2