Protein Overview: APM4

General Information:
Name(s) found:	APM4 / YOL062C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|984178\|emb\|CAA62522.1\| clathrin-associate protein YAP54 [Saccharomyces cerevisiae] [NCBI NR] gi\|6324510\|ref\|NP_014579.1\| Mu2-like subunit of the clathrin associated protein complex (AP-2); involved in vesicle transport; Apm4p [Saccharomyces cerevisiae] [NCBI NR] gi\|2492680\|sp\|Q99186.1\|AP2M_YEAST RecName: Full=AP-2 complex subunit mu; AltName: Full=Mu2-adaptin; AltName: Full=Plasma membrane adaptor AP-2 50 kDa protein; AltName: Full=Adaptin medium chain APM4; AltName: Full=Clathrin assembly protein complex 2 mediu [NCBI NR] pir\|\|S61715 clathrin-associated protein complex medium chain APM1 - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|1419879\|emb\|CAA99071.1\| APM4 [Saccharomyces cerevisiae] [NCBI NR] (Q99186) Adaptin medium chain homolog APM4 [Swiss-Prot] AP-2 complex subunit mu OS=Saccharomyces cerevisiae GN=APM4 PE=1 SV=1 [Swiss-Prot] APM4 AP-2 complex subunit, mu2 subunit, 55 KD [mips] Mu2-like subunit of the clathrin associated protein complex (AP-2); involved in vesicle transport [SGD] Chr XV from 211994-210519, reverse complement [hmr_yeast5_DM3-1_pombe.fasta] Chr XV from 210519-211994, reverse complement [yeastorf3.fasta] APM4, Chr XV from 210519-211994, reverse complement [yeast_orfs2] APM4 SGDID:S0005423, Chr XV from 211994-210519, reverse complement, Verified ORF [yeastflysequest.fasta] Apm4p [Saccharomyces cerevisiae]gi\|984178\|emb\|CAA62522.1\| clathrin-associate protein YAP54 [Saccharomyces cerevisiae]gi\|1419879\|emb\|CAA99071.1\| APM4 [Saccharomyces cerevisiae]gi\|2131214\|pir\|\|S61715 clathrin-associated protein complex medium chain APM1 [nrdb_11152004_Chlamydomonas] APM4 SGDID:S000005423, Chr XV from 211994-210519, reverse complement, Verified ORF, "Mu2-like subunit of the clathrin associated protein complex (AP-2); involved in vesicle transport" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] APM4 SGDID:S000005423, Chr XV from 211995-210520, reverse complement, Verified ORF, "Mu2-like subunit of the clathrin associated protein complex (AP-2); involved in vesicle transport" [yeast-contam.fasta] Mu2-like subunit of the clathrin associated protein complex (AP-2); involved in vesicle transport; Apm4p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] clathrin-associate protein YAP54 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] AP-2 complex subunit mu (Clathrin coat assembly protein AP50) (Clathrin coat-associated protein AP50) (Plasma membrane adaptor AP-2 50 kDa protein) (Clathrin assembly protein complex 2 medium chain) (Adaptin medium chain APM4) [nr-271106-contam.fasta] APM4 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] [chemostatdb.fasta] AP-2 complex subunit mu OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=APM4 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] APM4 SGDID:S000005423, Chr XV from 211995-210520, Genome Release 64-1-1, reverse complement, Verified ORF, "Mu2-like subunit of the clathrin associated protein complex (AP-2); involved in vesicle transport" [yeast-030211-contam.fasta] APM4 SGDID:S000005423, Chr XV from 211995-210520, Genome Release 64-2-1, reverse complement, Verified ORF, "Cargo-binding mu subunit of AP-2; AP-2 is a heterotetrameric endocytic cargo-binding adaptor that facilitates uptake of membrane proteins during clathrin-mediated endocytosis; Apm4p is required for AP-2 function and localization, and binds cell wall stress receptor Mid2p; AP-2 is required for cell polarity responses to pheromone, nutritional status and cell wall damage in S. cerevisiae, and for hyphal growth in C. albicans; AP-2 complex is conserved in mammals" [20141208_orf_trans.fasta] YOL062C SGDID:S000005423, Chr XV from 211995-210520, Genome Release 64-1-1, reverse complement, Verified ORF, "Mu2-like subunit of the clathrin associated protein complex (AP-2); involved in vesicle transport" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	491 amino acids
Gene Ontology:
Cellular Component:	AP-2 adaptor complex [ISS ]
Biological Process:	vesicle-mediated transport [TAS] intracellular protein transport [ISS]
Molecular Function:	molecular_function [ND]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MISGVLVYSS RGELVLNKFF KNSLKRSISD IFRVQVINNL DVRSPVLTLG STTFHHIRSR  60
   61 HGDNLWLVTI TRSNANSAAI WEFLYKLDAV MNAYRLDREE ALKEEFMIVH EMLDIMLGGN 120
  121 GIPIDTELNS VIAQMSVKPV RNMGGLLDSP DGNDVLSSSS SPTSSAGELH FPKFLTKRSS 180
  181 SFLGQGDSTS DFYDNNKITW RPKGIIHKKD EVFLYVNERI NILVSRDGSI LKSYVDGTID 240
  241 ITTHLSGTPI CRFGLNDSLG MQSEDEKKWL AQQQRHSGSD FGNKNFIPKA AAGSVLLEDC 300
  301 KFHECVSLDK FNRNHIIEFV PPDGSMELMK YHVRDNINLP FKVTPIVTHS TRDNEIDYRI 360
  361 TLKSLFPGKL SAKDVVLHIP VPPSTVDCKI SVSNGHCKFV PEENAMIWRF NKYNGLTENT 420
  421 LSAVTVSTSD TTQLNLQQWT RPPISLEFEV MMFSNSGLVV RYFTISGKDS KHRAVKWIKY 480
  481 ISKAGSYEVR Y

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		APL1 APM4 APS2
View Details	(MIPS) Mewes HW, et al. (2004)		APL1 APL3 APM4 APS2
View Details	Krogan NJ, et al. (2006)		APM4 APS2
View Details	Gavin AC, et al. (2006)		APL1 APM4
View Details	Gavin AC, et al. (2002)		APL1 APL3 APL5 APL6 APM3 APM4 APS2 APS3 CKB1 ENO2
View Details	Ho Y, et al. (2002)		APM4 SEC53
View Details	Qiu et al. (2008)		APL1 APL3 APM4

Mass Spectrometry Data

No mass spectrometry results found for this protein.

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	HRT1	APM4	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	APM4	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..179]	PSI-BLAST	842.9794	Second domain of Mu2 adaptin subunit (ap50) of ap2 adaptor; Mu2 adaptin (clathrin coat assembly protein AP50)
2	View Details	[180..491]	PSI-BLAST	842.9794	Second domain of Mu2 adaptin subunit (ap50) of ap2 adaptor; Mu2 adaptin (clathrin coat assembly protein AP50)

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.88

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2