Protein Overview: YNL247W

General Information:
Name(s) found:	YNL247W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6324082\|ref\|NP_014152.1\| Essential protein of unknown function; may interact with ribosomes, based on co-purification experiments; Ynl247wp [Saccharomyces cerevisiae] [NCBI NR] pir\|\|S63220 probable membrane protein YNL247w - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|1730840\|sp\|P53852.1\|SYC_YEAST RecName: Full=Cysteinyl-tRNA synthetase; AltName: Full=Cysteine--tRNA ligase; Short=CysRS [NCBI NR] gi\|1302287\|emb\|CAA96154.1\| unnamed protein product [Saccharomyces cerevisiae] [NCBI NR] gi\|1255971\|emb\|CAA65497.1\| unnamed protein product [Saccharomyces cerevisiae] [NCBI NR] Cysteinyl-tRNA synthetase OS=Saccharomyces cerevisiae GN=YNL247W PE=1 SV=1 [Swiss-Prot] Cysteinyl-tRNA synthetase [mips] Cysteinyl-tRNA synthetase; may interact with ribosomes, based on co-purification experiments [SGD] Chr XIV from 182874-185177 [yeastorf3.fasta] (P53852) Cysteinyl-tRNA synthetase (EC 6.1.1.16) (Cysteine--tRNA ligase) (CysRS) [4932.FASTACCERV.txt] YNL247W, Chr XIV from 182874-185177 [yeast_orfs2] YNL247W SGDID:S0005191, Chr XIV from 182874-185177, Uncharacterized ORF [yeastflysequest.fasta] Protein required for cell viability [Saccharomyces cerevisiae]gi\|1255971\|emb\|CAA65497.1\| unnamed protein product [Saccharomyces cerevisiae]gi\|1302287\|emb\|CAA96154.1\| unnamed protein product [Saccharomyces cerevisiae]gi\|2132775\|pir\|\|S63220 probable memb [nrdb_11152004_Chlamydomonas] YNL247W SGDID:S000005191, Chr XIV from 182876-185179, Uncharacterized ORF, "Protein required for cell viability" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] YNL247W SGDID:S000005191, Chr XIV from 182876-185179, Verified ORF, "Essential protein of unknown function; may interact with ribosomes, based on co-purification experiments" [SGD_S-cerevisiae_na_08-19-2008_con_reversed.fasta] YNL247W SGDID:S000005191, Chr XIV from 182876-185179, Verified ORF, "Cysteinyl-tRNA synthetase; may interact with ribosomes, based on co-purification experiments" [yeast-200209-contam.fasta] Cysteinyl-tRNA synthetase (Cysteine--tRNA ligase) (CysRS) [nr-271106-contam.fasta] Protein required for cell viability; Ynl247wp [Saccharomyces cerevisiae] [nr-271106-contam.fasta] unnamed protein product [Saccharomyces cerevisiae] [nr-271106-contam.fasta] [chemostatdb.fasta] Cysteine--tRNA ligase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=YNL247W PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] YNL247W SGDID:S000005191, Chr XIV from 182875-185178, Genome Release 64-1-1, Verified ORF, "Cysteinyl-tRNA synthetase; may interact with ribosomes, based on co-purification experiments" [yeast-030211-contam.fasta] YNL247W SGDID:S000005191, Chr XIV from 182875-185178, Genome Release 64-2-1, Verified ORF, "Cysteinyl-tRNA synthetase; may interact with ribosomes, based on co-purification experiments" [20141208_orf_trans.fasta] TR7548\|c0_g1_i1\|g.5267 ORF TR7548\|c0_g1_i1\|g.5267 TR7548\|c0_g1_i1\|m.5267 type:complete len:768 (+) TR7548\|c0_g1_i1:56-2359(+) [Y12_S_cerevisiae-Sep2015-contam.fasta] TR6593\|c0_g1_i1\|g.4788 ORF TR6593\|c0_g1_i1\|g.4788 TR6593\|c0_g1_i1\|m.4788 type:complete len:768 (+) TR6593\|c0_g1_i1:76-2379(+) [DBVPG6044_S_cerevisiae-Sep2015-contam.fasta] asmbl_5424\|g.4193 ORF asmbl_5424\|g.4193 asmbl_5424\|m.4193 type:complete len:768 (+) asmbl_5424:76-2379(+) [DBVPG6044_Trinity_v2_2_0_PASA_6May2016-contam.fasta] asmbl_5425\|g.3789 ORF asmbl_5425\|g.3789 asmbl_5425\|m.3789 type:complete len:768 (+) asmbl_5425:76-2379(+) [DBVPG6044_Trinity_v2_2_0_PASA_6May2016-contam.fasta] asmbl_5873\|g.4490 ORF asmbl_5873\|g.4490 asmbl_5873\|m.4490 type:complete len:768 (+) asmbl_5873:135-2438(+) [Y12_Trinity_v2_2_0_PASA_6May2016-contam.fasta] ID=asmbl_5422\|g.3539 ORF ID=asmbl_5422\|g.3539 ID=asmbl_5422\|m.3539 type:complete len:768 (+) ID=asmbl_5422:76-2379(+) [DBVPG6044_quiverGenomes_Trinity_v2_2_0_PASA_2June2016-contam.fasta] ID=asmbl_5423\|g.3541 ORF ID=asmbl_5423\|g.3541 ID=asmbl_5423\|m.3541 type:complete len:768 (+) ID=asmbl_5423:76-2379(+) [DBVPG6044_quiverGenomes_Trinity_v2_2_0_PASA_2June2016-contam.fasta] ID=asmbl_5812\|g.3586 ORF ID=asmbl_5812\|g.3586 ID=asmbl_5812\|m.3586 type:complete len:768 (+) ID=asmbl_5812:135-2438(+) [Y12_quiver_Trinity_v2_2_0_PASA_2June2016-contam.fasta]
Organism:	Saccharomyces cerevisiae
Length:	767 amino acids
Gene Ontology:
Cellular Component:	cytoplasm [IDA] ribosome [IDA]
Biological Process:	ribosome biogenesis [RCA] cysteine metabolic process [NAS] cysteinyl-tRNA aminoacylation [IDA]
Molecular Function:	cysteine-tRNA ligase activity [IDA]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MNIFIKALRR YTIMSTPKIV QPKWKVPTPQ AKETVLKLYN SLTRSKVEFI PQSGNRGVTW  60
   61 YSCGPTVYDA SHMGHARNYV SIDINRRIIQ DYFGYDVQFV QNVTDIDDKI ILRARQNYLF 120
  121 DNFVKENDTK FNATVVDKVK TALFQYINKN FTIQGSEIKT IEEFETWLSN ADTETLKLEN 180
  181 PKFPMHVTAV QNAIESITKG DSMDAEVAFE KVKDVTVPLL DKELGSTISN PEIFRQLPAY 240
  241 WEQKFNDDML SLNVLPPTVT TRVSEYVPEI IDFVQKIIDN GYAYATSDGS VYFDTLKFDK 300
  301 SPNHDYAKCQ PWNKGQLDLI NDGEGSLSNF ADNGKKSNND FALWKASKAG EPEWESPWGK 360
  361 GRPGWHIECS VMASDILGSN IDIHSGGIDL AFPHHDNELA QSEARFDNQQ WINYFLHTGH 420
  421 LHIEGQKMSK SLKNFITIQE ALKKFSPRQL RLAFASVQWN NQLDFKESLI HEVKSFENSM 480
  481 NNFFKTIRAL KNDAASAGHI SKKFSPLEKE LLADFVESES KVHSAFCDNL STPVALKTLS 540
  541 ELVTKSNTYI TTAGAALKIE PLIAICSYIT KILRIIGFPS RPDNLGWAAQ AGSNDGSLGS 600
  601 LEDTVMPYVK CLSTFRDDVR SLAIKKAEPK EFLQLTDKIR NEDLLNLNVA LDDRNGQSAL 660
  661 IKFLTNDEKL EIVKLNEEKH ANELAKKQKK LEQQKLREQK ENERKQKAQI KPQDMFKDVT 720
  721 LYSAWDEQGL PTKDKDGNDI TKSMTKKLKK QWEQQKKLHE EYFGEDK

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Krogan NJ, et al. (2006)		GSP1 SRM1 YNL247W

Mass Spectrometry Data

No mass spectrometry results found for this protein.

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	MLC1	YNL247W	2	Unpublished Fields Lab Data
View Screen	DCN1	YNL247W	2	Unpublished Fields Lab Data
View Screen	CDC13	YNL247W	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	YNL247W	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..153]	PSI-BLAST	2.045757	No description for 1lleA_ was found.
2	View Details	[154..419]	PSI-BLAST	588.54902	Cysteinyl-tRNA synthetase (CysRS)
3	View Details	[420..474]	PSI-BLAST	588.54902	Cysteinyl-tRNA synthetase (CysRS)
4	View Details	[475..664]	PSI-BLAST	588.54902	Cysteinyl-tRNA synthetase (CysRS)
5	View Details	[665..767]	deduced	N/A	No confident structure predictions are available.

Functions predicted (by domain):

Gene Ontology predictions

No functions predicted.

Term	Confidence	Notes
ligase activity, forming aminoacyl-tRNA and related compounds	7.16276509815667	bayes_pls_golite062009
ligase activity, forming carbon-oxygen bonds	7.16276509815667	bayes_pls_golite062009
aminoacyl-tRNA ligase activity	7.13957775963187	bayes_pls_golite062009
ligase activity	6.11211476973434	bayes_pls_golite062009
cysteine-tRNA ligase activity	5.23703961447809	bayes_pls_golite062009
isoleucine-tRNA ligase activity	3.76808520367507	bayes_pls_golite062009
glutamate-tRNA ligase activity	2.63681738794687	bayes_pls_golite062009
catalytic activity	2.37674226834122	bayes_pls_golite062009
methionine-tRNA ligase activity	2.01261843164558	bayes_pls_golite062009
tyrosine-tRNA ligase activity	1.88984817882585	bayes_pls_golite062009
tryptophan-tRNA ligase activity	1.14187887833901	bayes_pls_golite062009
arginine-tRNA ligase activity	1.13743634264647	bayes_pls_golite062009
nucleotidyltransferase activity	0.89131347796645	bayes_pls_golite062009
transferase activity, transferring phosphorus-containing groups	0.625793556381714	bayes_pls_golite062009
nucleic acid binding	0.369886886936128	bayes_pls_golite062009
RNA binding	0.306683553109855	bayes_pls_golite062009
glutamine-tRNA ligase activity	0.118292082006187	bayes_pls_golite062009
binding	0.090743795600025	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2