Protein Overview: PPR1

General Information:
Name(s) found:	PPR1 / YLR014C [SGD]
Description(s) found: SHOW ONLY BEST	pir\|\|RGBYP1 regulatory protein PPR1 - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|6323042\|ref\|NP_013114.1\| Zinc finger transcription factor containing a Zn(2)-Cys(6) binuclear cluster domain, positively regulates transcription of genes involved in uracil biosynthesis; activity may be modulated by interaction with Tup1p; Ppr1p [Sacch [NCBI NR] gi\|4215\|emb\|CAA25876.1\| unnamed protein product [Saccharomyces cerevisiae] [NCBI NR] gi\|1360312\|emb\|CAA97536.1\| PPR1 [Saccharomyces cerevisiae] [NCBI NR] gi\|130784\|sp\|P07272.1\|PPR1_YEAST RecName: Full=Pyrimidine pathway regulatory protein 1 [NCBI NR] Pyrimidine pathway regulatory protein 1 OS=Saccharomyces cerevisiae GN=PPR1 PE=1 SV=1 [Swiss-Prot] PPR1 Transcription factor regulating pyrimidine pathway [mips] Zinc finger transcription factor containing a Zn(2)-Cys(6) binuclear cluster domain, positively regulates transcription of genes involved in uracil biosynthesis; activity may be modulated by interaction with Tup1p [SGD] PPR1, Chr XII from 172267-174981, reverse complement [yeast_orfs2] Chr XII from 172267-174981, reverse complement [yeastorf3.fasta] Chr XII from 174981-172267, reverse complement [yeastorf5.fasta] (P07272) Pyrimidine pathway regulatory protein 1 [4932.FASTACCERV.txt] PPR1 SGDID:S0004004, Chr XII from 174981-172267, reverse complement, Verified ORF [yeastflysequest.fasta] Ppr1p [Saccharomyces cerevisiae]gi\|4215\|emb\|CAA25876.1\| unnamed protein product [Saccharomyces cerevisiae]gi\|1360312\|emb\|CAA97536.1\| PPR1 [Saccharomyces cerevisiae]gi\|73248\|pir\|\|RGBYP1 regulatory protein PPR1 - yeast (Saccharomyces cerevisiae)gi\|13078 [nrdb_11152004_Chlamydomonas] PPR1 SGDID:S000004004, Chr XII from 174981-172267, reverse complement, Verified ORF, "Zinc finger transcription factor containing a Zn(2)-Cys(6) binuclear cluster domain, positively regulates transcription of genes involved in uracil biosynthesis; activit [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] Zinc finger transcription factor containing a Zn(2)-Cys(6) binuclear cluster domain, positively regulates transcription of genes involved in uracil biosynthesis; activity may be modulated by interaction with Tup1p; Ppr1p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Pyrimidine pathway regulatory protein 1 [nr-271106-contam.fasta] PPR1 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] unnamed protein product [Saccharomyces cerevisiae] [nr-271106-contam.fasta] PPR1 SGDID:S000004004, Chr XII from 174981-172267, reverse complement, Verified ORF, "Zinc finger transcription factor containing a Zn(2)-Cys(6) binuclear cluster domain, positively regulates transcription of genes involved in uracil biosynthesis; activity may be modulated by interaction with Tup1p" [yeast-bovrabprots.fasta] [chemostatdb.fasta] Pyrimidine pathway regulatory protein 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=PPR1 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] PPR1 SGDID:S000004004, Chr XII from 174982-172268, Genome Release 64-1-1, reverse complement, Verified ORF, "Zinc finger transcription factor containing a Zn(2)-Cys(6) binuclear cluster domain, positively regulates transcription of genes involved in uracil biosynthesis; activity may be modulated by interaction with Tup1p" [yeast-030211-contam.fasta] PPR1 SGDID:S000004004, Chr XII from 174982-172268, Genome Release 64-2-1, reverse complement, Verified ORF, "Zinc finger transcription factor; contains a Zn(2)-Cys(6) binuclear cluster domain, positively regulates transcription of URA1, URA3, URA4, and URA10, which are involved in de novo pyrimidine biosynthesis, in response to pyrimidine starvation; activity may be modulated by interaction with Tup1p" [20141208_orf_trans.fasta] YLR014C SGDID:S000004004, Chr XII from 174982-172268, Genome Release 64-1-1, reverse complement, Verified ORF, "Zinc finger transcription factor containing a Zn(2)-Cys(6) binuclear cluster domain, positively regulates transcription of genes involved in uracil biosynthesis; activity may be modulated by interaction with Tup1p" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	904 amino acids
Gene Ontology:
Cellular Component:	nucleus [TAS]
Biological Process:	regulation of transcription, DNA-dependent [TAS] uracil biosynthetic process [TAS]
Molecular Function:	transcription factor activity [TAS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MKQKKFNSKK SNRTDLSKRG DSPNIGISKS RTACKRCRLK KIKCDQEFPS CKRCAKLEVP  60
   61 CVSLDPATGK DVPRSYVFFL EDRLAVMMRV LKEYGVDPTK IRGNIPATSD DEPFDLKKYS 120
  121 SVSSLGEEGI LPHNGLLADY LVQKGNSMAS SAITSKSMAS PQTINVQRKE FLVNSKKQDG 180
  181 SALLPETGSP MTSDARAEEL RRCNKEISAL GTMRESSFNS FLGDSSGISF AKLVFTATNF 240
  241 RQDSGDDVLD EDIKQREQKY NGYAEAENNP HFDPLELPPR HAAEVMISRF FVDTNSQLPL 300
  301 LHRELFLKKY FEPIYGPWNP NIALASDQTG INSAFEIPIT SAFSAHTEPK RENVTEKIDV 360
  361 CSSVDVPWYD TWETSQKVNM RPIVELPTKF HIPYFFLNII FAIGHATQVL KSDITTVATY 420
  421 KRRATKYIAS LFSSSDRLEA LAGTLLMVIY SIMRPNVPGV WYTMGSVLRL TVDLGLHSEK 480
  481 INKNYDAFTR EIRRRLFWCV YSLDRQICSY FGRPFGIPEE SITTRYPSLL DDSFITLTNR 540
  541 EIDDYSDLPS PNPSSKVIAL AMYKIRRIQA SIVRILYAPG AELPRRFMDL ESWRIETYNE 600
  601 LERWFQVDVP KNFEMMNCKF NSIWFDLNYH YSKSILYGLS PKFPTLNDTA FKIVLDSTKG 660
  661 TIDVFYNLCV NKKIGYTWVA VHNMFMTGMT YLYVNFYSKN NINDCQEKVS EYTEKVLIVL 720
  721 KNLIGFCESA KTCYTSYKIL SSVVIKLKFM QINDAKGIFS DSNPLTSQAN RMSSYDKKTN 780
  781 VLGFDDGTFD NKVFNRTNFE EKAPFDIPLD EFFTELEKHS NVSQFNTLDV SEGNQVINES 840
  841 ASTNTSSALN CQSYTNNQDI MDILFQVTSG SVWDEFFVRS GNGNEGESSY DISKGKNSES 900
  901 GGIF

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Krogan NJ, et al. (2006)		BBC1 PPR1 SKT5 UBP7

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	MLP2	#24 Asynchronous Prep3-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#03 Alpha Factor Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	CLB2	PPR1	2	Unpublished Fields Lab Data
View Screen	MSN5	PPR1	1	Unpublished Fields Lab Data
View Screen	ALG13	PPR1	1	Hazbun TR, et al. (2003)
View Screen	TAM41	PPR1	1	Hazbun TR, et al. (2003)
View Screen	PSF2	PPR1	1	Hazbun TR, et al. (2003)
View Screen	GPI14	PPR1	1	Hazbun TR, et al. (2003)
View Screen	MIA40	PPR1	1	Hazbun TR, et al. (2003)
View Screen	LOS1	PPR1	1	Unpublished Fields Lab Data
View Screen	NSE5	PPR1	1	Hazbun TR, et al. (2003)
View Screen	TEM1	PPR1	1	Unpublished Fields Lab Data
View Screen	UBC7	PPR1	1	Unpublished Fields Lab Data
View Screen	YIF1	PPR1	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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No microscopy data found in the PDR for this protein.

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..133]	PSI-BLAST	533.751666	PPR1
2	View Details	[134..272]	deduced	N/A	No confident structure predictions are available.
3	View Details	[273..618]	MSA	5.104986	View MSA. No confident structure predictions are available.
4	View Details	[619..904]	deduced	N/A	No confident structure predictions are available.
5	View Details	[537..797]	MSA	3.165997	View MSA. No confident structure predictions are available.
6	View Details	[798..904]	deduced	N/A	No confident structure predictions are available.

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
transcription regulator activity	4.56444258780502	bayes_pls_golite062009
DNA binding	4.26146437716972	bayes_pls_golite062009
nucleic acid binding	4.17369933547911	bayes_pls_golite062009
transcription factor activity	3.049435548132	bayes_pls_golite062009
binding	2.91929847152301	bayes_pls_golite062009
RNA polymerase II transcription factor activity	2.03201524223606	bayes_pls_golite062009
sequence-specific DNA binding	1.87955444697212	bayes_pls_golite062009
specific RNA polymerase II transcription factor activity	1.86200506906385	bayes_pls_golite062009
transcription activator activity	0.915626626047549	bayes_pls_golite062009

Term	Confidence	Notes
transcription regulator activity	4.34861813464065	bayes_pls_golite062009
DNA binding	4.03985756894684	bayes_pls_golite062009
nucleic acid binding	3.97978190843512	bayes_pls_golite062009
transcription factor activity	2.87348461329489	bayes_pls_golite062009
binding	2.82809978070212	bayes_pls_golite062009
RNA polymerase II transcription factor activity	1.80191339934741	bayes_pls_golite062009
sequence-specific DNA binding	1.67344049333457	bayes_pls_golite062009
specific RNA polymerase II transcription factor activity	1.50774349911815	bayes_pls_golite062009
transcription activator activity	0.822069987038721	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.92

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2